Rmu_ssc0000098.1_g000001

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000098.1
OG0012256
Physical Location & Seq
Reverse (-)
180 .. 6572
6393 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
atgcttaggcattttgacaaggtcaaaccttcaagcacccccatgatcgtccgtagtcttgatcctgaaaaggatcttcttcgtctgaaggatgatgacgaagatgtgctagaggcagaagtgccttacttgaatatctttcagtacttgagatgtacgattgatatgagcttattctatccctacagagagatgatggattcggacccatcacacaccagaaacgccgccaacactggcttgcgttctctatccccatcccaaaacgatataagtgttttggaaggttttgttgatgctgggtacctctcagacccacacaaaggtcgctcccaaactggtcaagtgttcaccatgggaaaagaccttgatatcttggaggtctatagaacagaccgtagtcgttatatcttcgaaccatacataaattattgctcttcacgaagtggaagcccagcaacaacagtcgaagcctacaaagcaaattggcagcctcatctattatcggccaccatggacttgatgcacccggccacaaatggcaccacacctagcatcgtctcggccttttctgggatacccatttccagatccttggcattcatccctgaactacagctacaaacaaaaactctgatctgggcatttatcatcataaaggtgcttgcttgccttccagcttttacttttgagagaagcatggtgggggctttatctgtgatgggttcatccgtggtggactcacacacatgtccatgcttgtgcttggatgctctgcctacaactgccatgaatctcaagagtggaggagaacagaggaactcaatggccaagaagcatttagtgaaaccaggagggtctttggagttgagcagttcattcattgattcaggccatgacaggtggctctccatgaaagctcttccgggtatgaggaggaaacgaaagaatcgaaacttcgtgattgttaatgagcttgcaggccaatatgaggacagctttgaggatgttaagacgcaattactcaactattttacatacaaggctgtgaggactgtcatgaaccagctttatgagatgaacccaacacaatataggtggttgtatgactttgttgtgtcaaacaagcctggagatggtaagcggttcctccgcacacttgggaaggagaggcatgaacttgctgagagagtaatggtcacgcggcttcacctctatggtaaatgggtaaagaaatgtaaccatgctgaaatatatcaagaaatctctgatgagaacttggagttgatgcgtgaacggctttttgaaactgtgatatggccctcagatgactcaaacacagagaagattggctaa

Protein Analysis

451

Amino Acids

51.44

Weight (kDa)

6.84

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 338 - 435 3e-18 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19855 AT5G19855
fragaria_vesca FvH4_3g28850
malus_domestica MD03G1175000.v1.1 MD11G1193100.v1.1
prunus_persica Prupe.4G249500_v2.0.a1 Prupe.4G249500_v2.0.a1
pyrus_communis pycom03g13250 pycom11g16860
rosa_chinensis RchiOBHm_Chr5g0052051
rosa_laevigata RLG00000034846
rosa_multiflora Rmu_ssc0000098.1_g000001
rosa_roxburghii Rroxscaffold_1G00027850
rosa_rugosa Rorug05G0275200
rosa_samantha Rh5AG347200 Rh5BG358100 Rh5CG383600 Rh5DG373600
rosa_wichuraiana Rw5G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 303
AccB1I GGYRCC 2 cut(s) 303, 542
AccII CGCG 1 cut(s) 1204
AciI CCGC 4 cut(s) 228, 1144, 1153, 1204
AclWI GGATC 3 cut(s) 56, 81, 585
AcoI YGGCCR 3 cut(s) 507, 531, 828
AcuI CTGAAG 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 3 cut(s) 146, 157, 305
AfiI CCNNNNNNNGG 4 cut(s) 323, 573, 991, 1136
AflIII ACRYGT 1 cut(s) 749
AgsI TTSAA 3 cut(s) 33, 133, 1307
AjnI CCWGG 2 cut(s) 850, 1129
AluBI AGCT 7 cut(s) 171, 619, 680, 920, 976, 999, 1069
AluI AGCT 7 cut(s) 171, 619, 680, 920, 976, 999, 1069
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 3 cut(s) 56, 81, 585
AoxI GGCC 7 cut(s) 507, 531, 564, 828, 892, 982, 1319
ApeKI GCWGC 1 cut(s) 490
Asp718I GGTACC 1 cut(s) 303
AspS9I GGNCC 2 cut(s) 205, 1320
AsuC2I CCSGG 2 cut(s) 530, 927
AsuHPI GGTGA 2 cut(s) 343, 1202
AsuII TTCGAA 1 cut(s) 414
AvaII GGWCC 1 cut(s) 205
BalI TGGCCA 1 cut(s) 830
BanI GGYRCC 2 cut(s) 303, 542
BbvI GCAGC 1 cut(s) 502
BccI CCATC 5 cut(s) 190, 217, 265, 715, 1130
BceAI ACGGC 1 cut(s) 1313
BciT130I CCWGG 2 cut(s) 852, 1131
BciVI GTATCC 1 cut(s) 570
BcnI CCSGG 2 cut(s) 530, 927
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 2 cut(s) 110, 552
BfmI CTRYAG 3 cut(s) 184, 385, 614
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 3 cut(s) 228, 491, 1205
BlsI GCNGC 3 cut(s) 229, 492, 1206
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 4 cut(s) 530, 852, 927, 1131
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 205, 1320
BmiI GGNNCC 4 cut(s) 207, 305, 544, 1148
BmrFI CCNGG 4 cut(s) 530, 852, 927, 1131
BmsI GCATC 5 cut(s) 286, 513, 564, 760, 1278
BoxI GACNNNNGTC 1 cut(s) 399
BpmI CTGGAG 1 cut(s) 1152
Bpu10I CCTNAGC 1 cut(s) 5
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 169, 782
BpuMI CCSGG 2 cut(s) 530, 927
BsaJI CCNNGG 4 cut(s) 354, 513, 594, 732
Bsc4I CCNNNNNNNGG 4 cut(s) 323, 573, 991, 1136
Bse1I ACTGG 2 cut(s) 241, 343
BseBI CCWGG 2 cut(s) 852, 1131
BseDI CCNNGG 4 cut(s) 354, 513, 594, 732
BseGI GGATG 6 cut(s) 97, 257, 603, 728, 775, 1012
BseLI CCNNNNNNNGG 4 cut(s) 323, 573, 991, 1136
BseMII CTCAG 3 cut(s) 324, 1176, 1338
BseNI ACTGG 2 cut(s) 241, 343
BseRI GAGGAG 2 cut(s) 822, 949
BseXI GCAGC 1 cut(s) 502
BseYI CCCAGC 2 cut(s) 299, 454
Bsh1236I CGCG 1 cut(s) 1204
BshFI GGCC 7 cut(s) 509, 533, 566, 830, 894, 984, 1321
BshNI GGYRCC 2 cut(s) 303, 542
BsiSI CCGG 2 cut(s) 530, 926
BslI CCNNNNNNNGG 4 cut(s) 323, 573, 991, 1136
BsmAI GTCTC 1 cut(s) 565
BsmBI CGTCTC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 599
BsnI GGCC 7 cut(s) 509, 533, 566, 830, 894, 984, 1321
Bsp119I TTCGAA 1 cut(s) 414
Bsp143I GATC 5 cut(s) 45, 61, 73, 590, 636
Bsp19I CCATGG 2 cut(s) 354, 513
BspACI CCGC 4 cut(s) 228, 1144, 1153, 1204
BspANI GGCC 7 cut(s) 509, 533, 566, 830, 894, 984, 1321
BspCNI CTCAG 3 cut(s) 323, 1177, 1337
BspFNI CGCG 1 cut(s) 1204
BspHI TCATGA 1 cut(s) 1059
BspLI GGNNCC 4 cut(s) 207, 305, 544, 1148
BspPI GGATC 3 cut(s) 56, 81, 585
BspQI GCTCTTC 2 cut(s) 442, 927
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 2 cut(s) 303, 542
BsrI ACTGG 2 cut(s) 241, 343
BssECI CCNNGG 4 cut(s) 354, 513, 594, 732
BssMI GATC 5 cut(s) 45, 61, 73, 590, 636
BssT1I CCWWGG 3 cut(s) 354, 513, 594
Bst2UI CCWGG 2 cut(s) 852, 1131
Bst4CI ACNGT 4 cut(s) 398, 466, 1057, 1312
Bst6I CTCTTC 2 cut(s) 442, 927
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 5 cut(s) 242, 666, 670, 978, 982
BstDEI CTNAG 4 cut(s) 5, 310, 1185, 1324
BstDSI CCRYGG 3 cut(s) 354, 513, 732
BstF5I GGATG 6 cut(s) 97, 257, 603, 728, 775, 1012
BstFNI CGCG 1 cut(s) 1204
BstKTI GATC 5 cut(s) 48, 64, 76, 593, 639
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 5 cut(s) 45, 61, 73, 590, 636
BstMWI GCNNNNNNNGC 2 cut(s) 479, 1297
BstNI CCWGG 2 cut(s) 852, 1131
BstNSI RCATGY 1 cut(s) 753
BstPAI GACNNNNGTC 1 cut(s) 399
BstSCI CCNGG 4 cut(s) 528, 850, 925, 1129
BstSFI CTRYAG 3 cut(s) 184, 385, 614
BstUI CGCG 1 cut(s) 1204
BstV1I GCAGC 1 cut(s) 502
BstX2I RGATCY 2 cut(s) 73, 590
BstXI CCANNNNNNTGG 1 cut(s) 595
BstYI RGATCY 2 cut(s) 73, 590
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 7 cut(s) 509, 533, 566, 830, 894, 984, 1321
BtgI CCRYGG 3 cut(s) 354, 513, 732
BtsCI GGATG 6 cut(s) 97, 257, 603, 728, 775, 1012
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 5 cut(s) 242, 666, 670, 978, 982
CciI TCATGA 1 cut(s) 1059
Cfr13I GGNCC 2 cut(s) 205, 1320
CseI GACGC 1 cut(s) 1024
Csp6I GTAC 3 cut(s) 145, 156, 304
CspCI CAANNNNNGTGG 2 cut(s) 1079, 1114
CviQI GTAC 3 cut(s) 145, 156, 304
DdeI CTNAG 4 cut(s) 5, 310, 1185, 1324
DpnI GATC 5 cut(s) 47, 63, 75, 592, 638
DpnII GATC 5 cut(s) 45, 61, 73, 590, 636
DraIII CACNNNGTG 1 cut(s) 446
EaeI YGGCCR 3 cut(s) 507, 531, 828
Eam1104I CTCTTC 2 cut(s) 442, 927
EarI CTCTTC 2 cut(s) 442, 927
Eco130I CCWWGG 3 cut(s) 354, 513, 594
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 850, 1129
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 354, 513, 594
ErhI CCWWGG 3 cut(s) 354, 513, 594
Esp3I CGTCTC 1 cut(s) 565
Fnu4HI GCNGC 3 cut(s) 228, 491, 1205
FokI GGATG 6 cut(s) 104, 244, 590, 715, 782, 1019
Fsp4HI GCNGC 3 cut(s) 228, 491, 1205
FspBI CTAG 2 cut(s) 110, 552
GluI GCNGC 3 cut(s) 228, 491, 1205
GsaI CCCAGC 2 cut(s) 303, 458
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 7 cut(s) 509, 533, 566, 830, 894, 984, 1321
HapII CCGG 2 cut(s) 530, 926
HgaI GACGC 1 cut(s) 1024
HinfI GANTC 6 cut(s) 200, 740, 793, 887, 949, 1331
HpaII CCGG 2 cut(s) 530, 926
HphI GGTGA 2 cut(s) 343, 1202
Hpy166II GTNNAC 3 cut(s) 351, 739, 1295
Hpy188I TCNGA 6 cut(s) 87, 205, 313, 636, 1270, 1327
Hpy188III TCNNGA 8 cut(s) 59, 65, 441, 588, 799, 961, 1060, 1259
Hpy8I GTNNAC 3 cut(s) 351, 739, 1295
HpyAV CCTTC 5 cut(s) 39, 82, 278, 683, 1159
HpyCH4III ACNGT 4 cut(s) 398, 466, 1057, 1312
HpyCH4V TGCA 2 cut(s) 526, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 1297
HpyF3I CTNAG 4 cut(s) 5, 310, 1185, 1324
KpnI GGTACC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 45, 61, 73, 590, 636
LguI GCTCTTC 2 cut(s) 442, 927
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 5 cut(s) 286, 513, 564, 760, 1278
MaeI CTAG 2 cut(s) 110, 552
MaeIII GTNAC 2 cut(s) 1198, 1238
MalI GATC 5 cut(s) 47, 63, 75, 592, 638
MboI GATC 5 cut(s) 45, 61, 73, 590, 636
MboII GAAGA 6 cut(s) 68, 71, 113, 403, 429, 914
MflI RGATCY 2 cut(s) 73, 590
MlsI TGGCCA 1 cut(s) 830
MluCI AATT 3 cut(s) 427, 484, 1019
MluNI TGGCCA 1 cut(s) 830
MlyI GAGTC 2 cut(s) 734, 1325
Mox20I TGGCCA 1 cut(s) 830
MscI TGGCCA 1 cut(s) 830
MseI TTAA 2 cut(s) 969, 1011
MslI CAYNNNNRTG 6 cut(s) 41, 659, 748, 754, 760, 1215
Msp20I TGGCCA 1 cut(s) 830
MspI CCGG 2 cut(s) 530, 926
MspR9I CCNGG 4 cut(s) 530, 852, 927, 1131
Mva1269I GAATGC 1 cut(s) 599
MvaI CCWGG 2 cut(s) 852, 1131
MvnI CGCG 1 cut(s) 1204
MwoI GCNNNNNNNGC 2 cut(s) 479, 1297
NciI CCSGG 2 cut(s) 530, 927
NcoI CCATGG 2 cut(s) 354, 513
NdeII GATC 5 cut(s) 45, 61, 73, 590, 636
NlaIV GGNNCC 4 cut(s) 207, 305, 544, 1148
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 1 cut(s) 1198
NspI RCATGY 1 cut(s) 753
NspV TTCGAA 1 cut(s) 414
PagI TCATGA 1 cut(s) 1059
PciI ACATGT 1 cut(s) 749
PciSI GCTCTTC 2 cut(s) 442, 927
PcsI WCGNNNNNNNCGW 1 cut(s) 949
PctI GAATGC 1 cut(s) 599
PfeI GAWTC 4 cut(s) 200, 793, 887, 949
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 3 cut(s) 229, 492, 1206
PleI GAGTC 2 cut(s) 734, 1325
PpsI GAGTC 2 cut(s) 734, 1325
PscI ACATGT 1 cut(s) 749
PshAI GACNNNNGTC 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 850, 1129
PspFI CCCAGC 2 cut(s) 299, 454
PspGI CCWGG 2 cut(s) 850, 1129
PspN4I GGNNCC 4 cut(s) 207, 305, 544, 1148
PspPI GGNCC 2 cut(s) 205, 1320
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PsuI RGATCY 2 cut(s) 73, 590
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 3 cut(s) 146, 157, 305
RsaNI GTAC 3 cut(s) 145, 156, 304
RseI CAYNNNNRTG 6 cut(s) 41, 659, 748, 754, 760, 1215
SapI GCTCTTC 2 cut(s) 442, 927
SaqAI TTAA 2 cut(s) 969, 1011
SatI GCNGC 3 cut(s) 228, 491, 1205
Sau3AI GATC 5 cut(s) 45, 61, 73, 590, 636
Sau96I GGNCC 2 cut(s) 205, 1320
ScaI AGTACT 1 cut(s) 146
SchI GAGTC 2 cut(s) 734, 1325
ScrFI CCNGG 4 cut(s) 530, 852, 927, 1131
SfaNI GCATC 5 cut(s) 286, 513, 564, 760, 1278
SfcI CTRYAG 3 cut(s) 184, 385, 614
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 6 cut(s) 41, 659, 748, 754, 760, 1215
SmlI CTYRAG 2 cut(s) 148, 797
SmoI CTYRAG 2 cut(s) 148, 797
Sse9I AATT 3 cut(s) 427, 484, 1019
SsiI CCGC 4 cut(s) 228, 1144, 1153, 1204
SspMI CTAG 2 cut(s) 110, 552
StyD4I CCNGG 4 cut(s) 528, 850, 925, 1129
StyI CCWWGG 3 cut(s) 354, 513, 594
TaaI ACNGT 4 cut(s) 398, 466, 1057, 1312
TaqI TCGA 3 cut(s) 414, 468, 952
TasI AATT 3 cut(s) 427, 484, 1019
TatI WGTACW 1 cut(s) 144
TauI GCSGC 2 cut(s) 230, 1207
TfiI GAWTC 4 cut(s) 200, 793, 887, 949
Tru1I TTAA 2 cut(s) 969, 1011
Tru9I TTAA 2 cut(s) 969, 1011
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 1 cut(s) 1198
TseI GCWGC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 1198
TspDTI ATGAA 9 cut(s) 592, 717, 806, 867, 871, 929, 1076, 1094, 1191
TspGWI ACGGA 2 cut(s) 41, 721
TspRI CASTG 1 cut(s) 241
Tth111I GACNNNGTC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 205
XceI RCATGY 1 cut(s) 753
XspI CTAG 2 cut(s) 110, 552
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.