Rmu_ssc0000177.1_g000009

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000177.1
OG0016427
Physical Location & Seq
Forward (+)
62562 .. 81262
18701 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 10875 bp
atggcttccatggtgatccaaacctcctcaactactcctcctcctcctcctcctcctccacctttgaaccatccaataaaatatgatgtttttctcagttttagaggtgcggacacccgcacaaagtttacagaccatttatacaaggaattacatgttcgggaaggcatccccacctttagggattctgaagagctcgagaagggagaagacatttcacaactctttgaggcaattgaaaaatcgaaaattgcagttgtcattctttcaagtaactatgcttcttcgacatggtgtttgagagaagttgccaagattgccgaatgcagaaataagaccggactcatcgctttgccggtattctatgagatcgaaccaacccaggtacgaagccagactggaagtttcaagaaatactttgatgaatatgaagaggaatacaaggacagcatagagaaggtcgatatttggaggaaagcgttgactgaaataggtggtctctccggatgggatttaaaagacaggttggaaacctctgttattgaagagatagtaaaatgtgtcgagacaaaatggcagctcaaatttaatcctgcattctcaattgaggattccaaggattttgttgggatggatcgtgtgaggaaaatcgaattggacttggatatagagacattggataaaatcggtgtcattggaatttgcgggatgccaggagtcggtaagacgacgattgcagaagttgtgtttcgaagaaattggcaccattttgaagctaacagtttccttgctaatgttggagagataagtaagacgaaaggattattgcatatacagaagcaactttatcgcgacttgttggatggcaaagttataactatgaatgatgtccctgtggaaaggagaaaattgcgtcacaaaaaggtgcttatcattcttgatggtgtgaccgagttacaatctgtttttgagctagcaggaacagatgttaaagaattaatcaattggtttggtcgaggcagtagaatcattataaccacaataaatgaaaagttgttgaaggattatggggcaaaaacacacaaggttgagacactttctgaaaatgagcctcgtgtgctcttcttccggaaagccttcagcaaagaccatcctctgcatgattgtactgagttggagttcaagttcaagatagaccatactatgcgtcagtatgctgagttggcttacaaatttgtggatttaactgatggtcttcctttggctattgtagttttgggaagcttgttattgaacagatctttggatgaatgggaagctgaacttagcacactagaagctaataatgattctaatgatgagaaaacttttgggattcttaagctatgtattgatggattacgtgatggaggaaaggaaattttcttggacattgcatgcttctttaaaggagatgacaaaaatcgtgtggaaaggatatttgaaagctgcggactcaatccacgcattgaattgaacattctcatcgagaaatctctagtgacaattgtgggaggaaaattgtggatgcatgatttactacaaaaagctggccagaaaattgttattcgaaaatccgaattaccagagaatcgcagcagattgtgggatcatgaagaagccgtttcggtacttaaacagaataagggaacaagcgctgtcaaaggtattttcttaagctcacctcaactctgcaaagtgcgattgaaggccgactcattttcaaaaatggactttcttagattgttgaaaataaagaatgtgaaattttctggatgccatgaatacgatctttcaaataaattaagcttcttggaatggcatgactttccttcaacatttctgccatctgagtttgaaccgaagaatcttgttgagctgcacttgcctcgaagccaagtaaagcaattatgggaggataatataacgcctcctttggaaaagttggttgtaatggaccttagtgattgcaaagagttgaccactaccccagacttccagtcagtgccaaatcttgagagcctagtcctcaaaggttgtacaagattgtctgaggtagacctacccaatggagatcagctccaaaagctcatttcattgaatctggaaggttgcgagtctcttagtagtcttcccaacggtatctgcagcttgagatctctcacaactttcattctttcgggatgttccagacttgcaaatcttccggagaagattggggaaaacatgaaacaattatccggacttgatttggaggggacagctataaaagggctaccaacatcaattaagcacttgactggtcttattcagctcaatctaaaagactgcaaaaaccttttaagtctgccagccgtcatttgcagtttgacaaaacttcaagttctcaacctatctggctgctcaaatcttgctcagttaccagaaaacttgggaagtttagtaggtttgaaggaacttgatgcaagcagaagttgtataagacaagtaccttcctccattctacttctgaaggaccttgaaagtttatctttttgtggatgtggaggtatgccgccatcggcatgtcccatgggtttgcagttgcctgcttcattctcgggattagattctttgacaaaattggatctaagctattgcaaactgtctggtggagcaatccctgctgatcttgactgcttatcttcacttaatcatctaaatttaagtgggaacaatttactgagtgtaccggaaagcatcactaaactttctaaccttgaagtccttctattgaatgattgtagcgagcttcgatcattaccacatcttccaccaagtatacgacgtgtttttgcacggaattgtcctctgctcagaacttattcaaataaattaactgtgtggagttcaactgctacaggattttgtttcataaattcccaaagtagtgaagaacgtaaacttcagaggattccgatatcagaacaacgtcttctgcggcctcatttgcaaaagttctatgaggatataatttataaaggttgtggttttgaatatattactcctcacactgaaattccatcatggttcggtcgccggggtacagggtcgtctataacactccagctacctctagattatttcgatggcaaacgtgagttgatgggacttgctctatgttttgtttatgaagcgcaggagcatgagaatatggaatcaacagcttcttacatctcggctcaggaagtaaaatatagttgcaattacagttgtcgtttgtccacatatggaggtcctcatgatgactgtcagctcctcgtgtgtaaactcatggatcatgattatgttggatcttttcgacattgggtctatatagcaggagggctgtttgcagaaaggttgatcaaacgctctgttcgagctgaagttaaaatcaacagaccaggggtgaaagtgaaagcctgtggggcaagtctaatatactctggaggtgtgacagaatttgtttggaatttgaatctccgtgatgaaattcatatgagaaatctgtcgagtggggatcaaaaggaaaattctgacgatcagtttaataaacaagaacaagaaactattggtgatcatcagctggaactaaaacatgtacctgtccaatatcaggcccaaatcaaccacatgatctcattgtatgataccaagcgatcccttgatcataatgccggctcatctagtagaaccagcagcagcactgaagaacctcatcaaagattagaaataccaaaatccaatgatcagtccaacaaacaagatcaagagactaattatggttgtgctacagacaaaaaattcttgattgaaattgaaccacacaattcaagagatagttcaacttcaaccacgatctttgatgatcaattgagaacaaatgtagagtcattccttgaggaactatttgagctaggtggcaaagcacgtcgcaatgatttaggtttcattttccctctcagagcaattttgccgtggtttaatcatcaatgcattgggaacgtattcattccagaaaaagaaagaattgggagttcagtaagcctacctcttcctctagaattgtataacaataaggaatgggtaggatttgctgtatatgtggccttcactctgcctccaggtgtttcacttgaaaaatcatgttgcttcaagtctgtattgtatactagtgatggaaatttttattgcctaaaattatcggcggcttgtgaagacaattttaggggagcacatcgactccttatcatccacataccacgagggcgttttaaaaaacagttgaatgagtgcgtggcaattcgtgctgcttttgtctcctcaactccaagtatggaggctgagatgtgtgggatccgtgtagtatacgagcaagatttgaaagggttaatccaaacaattacccaatgcaccacaagagatccgcctgcagtttactatggactagctcattcattcgtgccagatggaagagattctttcatgttagctattatggaggataatttgattgaagcagctaaatccagcaaaaggaagctttcaaccttgcaagagcctttaacagattcaaagttcctcagtggtcaacaaatttacttagagatagaggctggaactccatacatgccacacaagttcaaaaattccatagttgactggaaaaaaaaccttgagcttttgcttgaatgtttctttaccctttctgatctatctattaatctcactcttcacagaagcacaacctctttctcaagggattttgatccttgttctggtattttccttcaaagagaaattccagagtggttcacaccacaatatccctccgagagtatggtgttggaacttcctccaaatttggaaaatgacttgaatttcaatggacttgttgtatgtgctgccttttctgtccatgcctctgaagctgacgtcctcaaatatctgaaatcaggactccatccccaacttacgtgccatttccaaaatttgagttctgaatcttctgatcaatcttatggtcttacagctcccatgattgataccagtcccgagaacaaattcatgtggttatatcgcagaggattcatttggcaggaatacctaccccgtgacttatttaaggatctgctgcataaagattcttcagtcaaggtttcatttaggagtgattcttcccaagtcttggaggtacagaaatgtagcttccgcatcttatacaagcaagatgtggaaaaattcaagcaaatgttaatagagtgctttgcctccttctttgacaattacgacatcattcgagcatatttagtacaggattctggagattacagccacttaaatcatcattcagtcacacctttttgtcagggtgagactagtggaaaagccaatgatcagctggaactaggacatgtacctgtccaatatcagccccaaatggaccgcatgatctctttgtatgatactaaagcaagtaacatgcaatcccttgaccatgatgcaggatcatctagtagaactggcagcagtactgaagaacctcttcaaatattagaaagaccagaatccaatgatctgtccgatgaacaagtgcacgaaactaattctgaccttacacatgtagcagaatgtggttatgcttcagacactaaattcttgattgaaattgaaccacacacttcaagagataattcaagttcaaccaagattgttggtgatcaattgagaacaaatgtagagtcattccttgaagaactatttgagctgggaggcaaagcacgtcgctatgatttaggtttcattttctctcttcgagcaattttaccgtggtttaaacatcaaggcattggggacatgataggtgtacctcttcctctagaattgtataacaacaaggaatgcataggatttgctctatatgtgatcttcactctgcccccaggtgcttggcttaaagaaggttcttgttttaaaattgagtttactgaagagccaaggtattgcctaaacttagcgtccgctggtgaagacaatttcaggggttcacatcgactcctggttattcacataccacaaagacattttggagaacagttgaaccagtggagtaccattcgtgcttcttttgaatcccttatgccaggtgtggaggttgagatgtgtggcatgcgtgtagtataccaggaaaatttgaaagggttaatccaaacaattaccaactgcacaatatgcagtccccctgcttactatggacttgcccaaccatttgtgccaggaaggtggtcttatgtgttcacacacatgaggaatagtttggttgaagcagctaaatccagcaagaggaagctttcaaccttgcaagagcctttaacagatttaaaatccatccaaggaattcagttactgatacttgctgcaactccatttacttcactcaagaaaacagaaacagtaatcaagtggaaaagaaaccttaagcttttgcttcaacgtttcttaaccctttctggtctatcaataagtctcattcttagaggaggcagaatttctttctcagaggatcataatcttcatagtggtattttctttcaaaaggaaattccagactggtttacaccacatcactctgccagtatggtgcccgaacttcctccagatttggaaaatgatatgaatttgatgggttttgttgtatgtgctgccttttcagtccacgcctctaaagctgatgtcctcaaatatctggattcgggacaccatcccgaacttatgtgccatttccaatcaagttctgactattctggtgtagtagttcgtaacatggttggcatcagtccgaagaacaaattcatgtggttttatcgtagtggattcatttggctgcattacgtgccccgtcacaatgttgagcatctcttcaataaactatctgaactaaaggtttcatttgagagtgattcttcccaagtattggcagtacagaaatgtagcttccgtttcttatacaagcaagatgtagaaggatttaagcaaacaataatagagtgctttacctccttctttgacaacgaggatatcattaagccatatatagaagaagaggactctggacataatgagtggccaaatcatggagatgagatgggaccatctagtacaactggtggcaacattgtcattgagaaaactcacaatgagagattagatgtacccataactgatgggaaggggaaaggcccttctgatgtctcctcagttgaagcagatgagagagattatcatggaactatacctgtttgtcagggtgaaactagtggacaagccagtcatcaactggaaccagttacacatgtagtagaagaagaacatggttctagtatttctcctcaacagctaatcccattcttatctgaaattgaacctcccgcctcaactgcaggttcaagttcaaccaaatttgaaggtgatcagttcagaactagtggaaaatgcagtgatcagctggaactagttacacatgtagtagaagaagaacacggttctagttgtcctccggagctaatcccattcttatttgaaattgaaccttccgcctcaacagattcaagttcaaccaaatttgaaggtgatcagttcagaacaaatgtggaggctttgctcacagtaatgtttcgacataagatcaaaggatatgactttgaacatggttttattttacctccgagagttggagcaattttgccatggtttaatcaccaaaccattgggagcgaagcactagtacttcatcttcctccggatttatacaacgataagaaatgggcagggttagctctatatgttgctttcactgtgcccccaggttatacggaaaacattctatgtcgctgttctttgtctacctctgcaggacatgcagaagcgatcacaatagcatgctcccaaggcaatttttccggatcacgacgactctgtttcattcataaaccgcgtgcacgttttccagaacagtggaatacgtgtagttacatttctgccttctttgaagtccttactccagttgtggatgttgagatgtgtggggtccatctactatacgagcaagatctgatagggataatccaaacaattacccactgcactatcaggagtccgcctgtttactatgggattggatttgctaacacaccacgtcagtcgcaaatgggtcagccttttgcggatataaatatggacacaaatttgaatgaactcgctgcaaggactccatctaccgctcaagagcctgtaaagacccttgttggtcaagtaattcatttacgcatacatggtgacaccccattcaagaaaaaagattcagtgatggagtggaaaaaaaaccttcagtttttgcttcaatgtttctttaggtttcctctatctataaccctcactcttggaggagacgtcatctctagcctaggtgattctgatccttactgtctctgcagctcaagtattatccctcaaaaggacattccgaagtggttctgtggggtgaaatatcacacaaataagttgaagaccacactccctccgaatttggaaaatgatgagaactggttgggatttgttgtctgtgctgcgttttcagtccatgcctcccaagatgacgtcctcagttatctggattcagaaaactatccgaaacttacatgccttttgcagtggggttctgaaagagctttgaatctcaaagctggcaagaagtcgagcattgttctcaggaatgaattcatgtggttatatcttagacgattcatttggctgcgatacataccccatcagaaatttaaggatcggttacatatatctgatcacctagaggcttcatttaggactgattcaatttcttccaaagtcttgaggatacaggaatgcagcctgcgtgttctatacaagaaagacgtggaagaatttaagcaaacaataatagagtgctttacctgcttctttgacaacgaggatgtcattcggccgtatatagaagagggttccagacataacaagtggccaagtcatgcagatgacagagattcttatggcactacacctgtttgtcagggtgaacaaggttctgcatccagtacttctccggagctaatgccatttttcattgaaactgaacctccagcttcaacggattcaagttcaaccaaagtcacacggcaactgagaatgatagagtcatcgcttgaacaactatttcaggggggcagcgcacgccactataattatggctttattttatctcctagagtcagagcagttttgccgtggtttagtcatcaaagcattgggaacgaagttaaaattaatgcccctccggatttgtataaagataataaatgggtaggctttgctctatatgttgcctttactcagtctccaggttcacatagttgctctatgtgtagtttgtctgtcttcccaggacattcagtacattccataaacacatgcctcgatgaagaccaattttccggatcacgaagactttgtttctttcatataccacgtgcgcgcttccctgagcagtgtgatcagtgtcctgtcatttttgctcggtttgatttccttagcccaggttcggaggttgaaatgtgtgggatccgtgtagtgtatgatgaagatataaaagggctagtccaaacaattactcagagcactatcaggagtcctccgcctttttacttctcccaagatgaaaaaacagaaatcttttccccggggaggcttttgctgcccgtacatatggccttgagtttggttgcttactccaaagcctgcatcctgcccacacgaagcgcacgaagtccactggttccatccatgaaatttggtaaagtaaacttggtcatacatggtgacactcaattcaggaaagatacaaccgagtggaaaagaaacttcgagattttgcttcaatctttcttcaggcttgatctgtccgtaaaactcgttctcggagaagacaccatcgctttctatcaggattgcttcaaccatgcttgtcttttccctcaaaagaagattccagattggttcaaagaaaaatctctcacatctacgtatgtgtacccacttcccgatgatttggaaagtgacaacaattggatgggacttgttatatgtgctgccttttcagtacacccacttccaactgaccttggcaattataaggactcagaactgagttctgatgttaagtgcaattttcggtggagttctaatccttatcgattcatttctatcaatgagggtgtgttcggcgtcaggccaaacaacaaattcatgtggttatctcgtagaggattcatttggctgcaattcatatctggtgaaatttttaagtttgccatggataaacatcgtcgtcaactagaagcttcatttgagagtggttccccagctttgtcggtacaggaatgtagctaccgaatcttatacaagcaagacttgcaagaatataagcaaacaattatacagtgcttcgcctctgtctttgacaacttggatcacagtctaccatataaggaagacgattctcgacataacgaggagccaattcatgaagatgatcagatggactctttaggaactacaagcttttctgaggacaccccccatggaagagaaacaacagtggttcttcctgaaagtgaaaccagtggaaattcctttaatcgtttcaacagcatactgtttgagcccattttgtacgacaacaaaacggcagtaattttcgctgattttcgaggatgggaagaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

3624

Amino Acids

410.46

Weight (kDa)

5.77

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 192 1.7e-45 TIR domain
TIR_2 PF13676 30 - 143 1.5e-12 TIR domain
NB-ARC PF00931 227 - 383 2.8e-12 NB-ARC domain
WHD_ROQ1 PF23282 472 - 537 4.6e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 702 - 812 8.6e-10 Leucine-rich repeat region
LRR_13 PF23286 731 - 817 1.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 785 - 865 2.4e-06 Leucine-rich repeat region
LRR_13 PF23286 818 - 974 1.4e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1029 - 1168 3.4e-08 C-JID domain
C-JID PF20160 1618 - 1766 8e-07 C-JID domain
C-JID PF20160 2218 - 2361 9.5e-07 C-JID domain
C-JID PF20160 2856 - 3005 9e-07 C-JID domain
C-JID PF20160 3374 - 3521 3.2e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 875, 1034, 3057, 10281
AasI GACNNNNNNGTC 1 cut(s) 7386
AatII GACGTC 3 cut(s) 4998, 8511, 8718
Acc36I ACCTGC 2 cut(s) 7490, 9056
AccB1I GGYRCC 2 cut(s) 762, 6700
AccB7I CCANNNNNTGG 3 cut(s) 5248, 7033, 7193
AccBSI CCGCTC 1 cut(s) 8339
AccI GTMKAC 7 cut(s) 2097, 2860, 4229, 4428, 6240, 7961, 10627
AccII CGCG 3 cut(s) 852, 8053, 9625
AclI AACGTT 1 cut(s) 6553
AcoI YGGCCR 4 cut(s) 1591, 7184, 9077, 9113
AcvI CACGTG 1 cut(s) 9620
AcyI GRCGYC 4 cut(s) 4995, 8508, 8715, 10374
AfeI AGCGCT 1 cut(s) 1696
AflII CTTAAG 3 cut(s) 1379, 1714, 6536
AflIII ACRYGT 8 cut(s) 154, 2866, 3658, 5473, 5680, 7411, 7579, 8081
AhdI GACNNNNNGTC 2 cut(s) 2038, 3386
AhlI ACTAGT 6 cut(s) 4232, 5438, 7373, 7541, 7570, 7840
AjiI CACGTC 5 cut(s) 2867, 3992, 5843, 8255, 9010
AjuI GAANNNNNNNTTGG 4 cut(s) 638, 670, 1286, 1318
AloI GAACNNNNNNTCC 2 cut(s) 1169, 1201
Alw21I GWGCWC 6 cut(s) 198, 1122, 4297, 5658, 8059, 9770
Alw44I GTGCAC 2 cut(s) 5654, 8055
AlwNI CAGNNNCTG 1 cut(s) 6466
Ama87I CYCGRG 4 cut(s) 197, 2638, 5114, 9829
Aor51HI AGCGCT 1 cut(s) 1696
ApaLI GTGCAC 2 cut(s) 5654, 8055
ArsI GACNNNNNNTTYG 8 cut(s) 280, 312, 3935, 3967, 5786, 5818, 8847, 8879
AseI ATTAAT 3 cut(s) 998, 4782, 9417
AspA2I CCTAGG 1 cut(s) 8521
AspLEI GCGC 6 cut(s) 1697, 3217, 9323, 9625, 9627, 9911
AspS9I GGNCC 8 cut(s) 1996, 2554, 3314, 3679, 5503, 7208, 7298, 8146
AsuC2I CCSGG 3 cut(s) 3119, 9830, 9831
AsuII TTCGAA 2 cut(s) 751, 1609
AsuNHI GCTAGC 1 cut(s) 973
AvaI CYCGRG 4 cut(s) 197, 2638, 5114, 9829
AvaII GGWCC 6 cut(s) 1996, 2554, 3314, 5503, 7208, 8146
AvrII CCTAGG 1 cut(s) 8521
BaeGI GKGCMC 5 cut(s) 5658, 6705, 6957, 7920, 8059
BalI TGGCCA 3 cut(s) 1593, 7186, 9115
BamHI GGATCC 2 cut(s) 4416, 9711
BanI GGYRCC 2 cut(s) 762, 6700
BanII GRGCYC 2 cut(s) 198, 10815
BarI GAAGNNNNNNTAC 8 cut(s) 265, 297, 7827, 7836, 7859, 7868, 10242, 10274
BauI CACGAG 3 cut(s) 1113, 3338, 4323
BbrPI CACGTG 1 cut(s) 9620
Bbv12I GWGCWC 6 cut(s) 198, 1122, 4297, 5658, 8059, 9770
BceAI ACGGC 7 cut(s) 1646, 2378, 4021, 9064, 9284, 9361, 10851
BcgI CGANNNNNNTGC 4 cut(s) 830, 864, 1910, 1944
BciVI GTATCC 1 cut(s) 8964
BcnI CCSGG 3 cut(s) 3119, 9830, 9831
BcuI ACTAGT 6 cut(s) 4232, 5438, 7373, 7541, 7570, 7840
BfmI CTRYAG 7 cut(s) 2185, 2937, 3852, 4491, 7497, 7968, 8548
BfoI RGCGCY 1 cut(s) 1698
BfrI CTTAAG 3 cut(s) 1379, 1714, 6536
BfuAI ACCTGC 2 cut(s) 7490, 9056
BfuI GTATCC 1 cut(s) 8964
BglI GCCNNNNNGGC 1 cut(s) 3488
BglII AGATCT 3 cut(s) 1298, 2195, 8167
BlnI CCTAGG 1 cut(s) 8521
BmcAI AGTACT 3 cut(s) 5593, 7845, 9190
Bme18I GGWCC 6 cut(s) 1996, 2554, 3314, 5503, 7208, 8146
BmeRI GACNNNNNGTC 2 cut(s) 2038, 3386
BmeT110I CYCGRG 4 cut(s) 197, 2638, 5114, 9829
BmgBI CACGTC 5 cut(s) 2867, 3992, 5843, 8255, 9010
BmgT120I GGNCC 8 cut(s) 1996, 2554, 3314, 3679, 5503, 7208, 7298, 8146
BmtI GCTAGC 1 cut(s) 977
BplI GAGNNNNNCTC 2 cut(s) 2103, 2135
BpmI CTGGAG 8 cut(s) 3128, 3528, 4167, 5403, 6699, 8103, 9216, 9474
Bpu10I CCTNAGC 3 cut(s) 3261, 9633, 9680
Bpu14I TTCGAA 2 cut(s) 751, 1609
BpuMI CCSGG 3 cut(s) 3119, 9830, 9831
Bsa29I ATCGAT 1 cut(s) 10342
BsaAI YACGTR 6 cut(s) 1403, 5037, 6952, 8082, 9620, 10173
BsaBI GATNNNNATC 4 cut(s) 4827, 6627, 8532, 10470
BsaHI GRCGYC 4 cut(s) 4995, 8508, 8715, 10374
BsaI GGTCTC 1 cut(s) 503
Bse118I RCCGGY 2 cut(s) 355, 3737
Bse3DI GCAATG 2 cut(s) 1431, 4003
Bse8I GATNNNNATC 4 cut(s) 4827, 6627, 8532, 10470
BseCI ATCGAT 1 cut(s) 10342
BseJI GATNNNNATC 4 cut(s) 4827, 6627, 8532, 10470
BseMI GCAATG 2 cut(s) 1431, 4003
BsePI GCGCGC 1 cut(s) 9623
BseSI GKGCMC 5 cut(s) 5658, 6705, 6957, 7920, 8059
BseX3I CGGCCG 1 cut(s) 9077
BseYI CCCAGC 2 cut(s) 5827, 10510
BsgI GTGCAG 3 cut(s) 1904, 6268, 8185
Bsh1236I CGCG 3 cut(s) 852, 8053, 9625
Bsh1285I CGRYCG 2 cut(s) 3115, 9080
BshNI GGYRCC 2 cut(s) 762, 6700
BshVI ATCGAT 1 cut(s) 10342
BsiEI CGRYCG 2 cut(s) 3115, 9080
BsiHKAI GWGCWC 6 cut(s) 198, 1122, 4297, 5658, 8059, 9770
BsiHKCI CYCGRG 4 cut(s) 197, 2638, 5114, 9829
BsmBI CGTCTC 1 cut(s) 8499
BsmI GAATGC 4 cut(s) 329, 596, 5966, 8984
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 4 cut(s) 197, 2638, 5114, 9829
Bsp119I TTCGAA 2 cut(s) 751, 1609
Bsp1407I TGTACA 1 cut(s) 2078
Bsp19I CCATGG 5 cut(s) 9, 2610, 7805, 10461, 10729
Bsp68I TCGCGA 1 cut(s) 852
BspDI ATCGAT 1 cut(s) 10342
BspFNI CGCG 3 cut(s) 852, 8053, 9625
BspHI TCATGA 4 cut(s) 1651, 3319, 3358, 10672
BspMAI CTGCAG 5 cut(s) 2189, 4495, 7501, 7972, 8552
BspMI ACCTGC 2 cut(s) 7490, 9056
BspOI GCTAGC 1 cut(s) 977
BspQI GCTCTTC 3 cut(s) 186, 1127, 6045
BspT104I TTCGAA 2 cut(s) 751, 1609
BspT107I GGYRCC 2 cut(s) 762, 6700
BspTI CTTAAG 3 cut(s) 1379, 1714, 6536
BspTNI GGTCTC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 8339
BsrDI GCAATG 2 cut(s) 1431, 4003
BsrFI RCCGGY 2 cut(s) 355, 3737
BsrGI TGTACA 1 cut(s) 2078
BssAI RCCGGY 2 cut(s) 355, 3737
BssHII GCGCGC 1 cut(s) 9623
BssNAI GTATAC 4 cut(s) 2861, 4230, 4429, 6241
BssNI GRCGYC 4 cut(s) 4995, 8508, 8715, 10374
BssSI CACGAG 3 cut(s) 1113, 3338, 4323
Bst1107I GTATAC 4 cut(s) 2861, 4230, 4429, 6241
Bst2BI CACGAG 3 cut(s) 1113, 3338, 4323
BstACI GRCGYC 4 cut(s) 4995, 8508, 8715, 10374
BstAFI CTTAAG 3 cut(s) 1379, 1714, 6536
BstAPI GCANNNNNTGC 6 cut(s) 2702, 4367, 6291, 6952, 7976, 10561
BstAUI TGTACA 1 cut(s) 2078
BstBAI YACGTR 6 cut(s) 1403, 5037, 6952, 8082, 9620, 10173
BstBI TTCGAA 2 cut(s) 751, 1609
BstDSI CCRYGG 8 cut(s) 9, 2610, 4037, 5888, 7805, 9377, 10461, 10729
BstFNI CGCG 3 cut(s) 852, 8053, 9625
BstH2I RGCGCY 1 cut(s) 1698
BstHHI GCGC 6 cut(s) 1697, 3217, 9323, 9625, 9627, 9911
BstMCI CGRYCG 2 cut(s) 3115, 9080
BstSFI CTRYAG 7 cut(s) 2185, 2937, 3852, 4491, 7497, 7968, 8548
BstSLI GKGCMC 5 cut(s) 5658, 6705, 6957, 7920, 8059
BstSNI TACGTA 1 cut(s) 10173
BstUI CGCG 3 cut(s) 852, 8053, 9625
BstX2I RGATCY 9 cut(s) 1298, 2195, 2665, 3371, 4416, 4483, 5188, 8167, 9711
BstXI CCANNNNNNTGG 4 cut(s) 6009, 6342, 7826, 8073
BstYI RGATCY 9 cut(s) 1298, 2195, 2665, 3371, 4416, 4483, 5188, 8167, 9711
BstZ17I GTATAC 4 cut(s) 2861, 4230, 4429, 6241
BstZI CGGCCG 1 cut(s) 9077
Bsu15I ATCGAT 1 cut(s) 10342
BsuI GTATCC 1 cut(s) 8964
BsuTUI ATCGAT 1 cut(s) 10342
BtgI CCRYGG 8 cut(s) 9, 2610, 4037, 5888, 7805, 9377, 10461, 10729
BtgZI GCGATG 3 cut(s) 331, 9276, 10066
BtrI CACGTC 5 cut(s) 2867, 3992, 5843, 8255, 9010
BtsI GCAGTG 4 cut(s) 7561, 8197, 8774, 9644
BtuMI TCGCGA 1 cut(s) 852
BveI ACCTGC 2 cut(s) 7490, 9056
CaiI CAGNNNCTG 1 cut(s) 6466
CciI TCATGA 4 cut(s) 1651, 3319, 3358, 10672
CfoI GCGC 6 cut(s) 1697, 3217, 9323, 9625, 9627, 9911
Cfr10I RCCGGY 2 cut(s) 355, 3737
Cfr13I GGNCC 8 cut(s) 1996, 2554, 3314, 3679, 5503, 7208, 7298, 8146
Cfr9I CCCGGG 1 cut(s) 9829
ClaI ATCGAT 1 cut(s) 10342
CseI GACGC 4 cut(s) 902, 1196, 6066, 10363
CspCI CAANNNNNGTGG 2 cut(s) 3904, 3939
DraI TTTAAA 6 cut(s) 516, 1447, 4336, 5896, 6034, 6441
DrdI GACNNNNNNGTC 1 cut(s) 7386
DriI GACNNNNNGTC 2 cut(s) 2038, 3386
DseDI GACNNNNNNGTC 1 cut(s) 7386
EaeI YGGCCR 4 cut(s) 1591, 7184, 9077, 9113
EagI CGGCCG 1 cut(s) 9077
Eam1105I GACNNNNNGTC 2 cut(s) 2038, 3386
EciI GGCGGA 4 cut(s) 4476, 7642, 8205, 9774
Ecl136II GAGCTC 1 cut(s) 196
EclXI CGGCCG 1 cut(s) 9077
Eco105I TACGTA 1 cut(s) 10173
Eco24I GRGCYC 2 cut(s) 198, 10815
Eco31I GGTCTC 1 cut(s) 503
Eco32I GATATC 2 cut(s) 3000, 7138
Eco47I GGWCC 6 cut(s) 1996, 2554, 3314, 5503, 7208, 8146
Eco47III AGCGCT 1 cut(s) 1696
Eco52I CGGCCG 1 cut(s) 9077
Eco53kI GAGCTC 1 cut(s) 196
Eco72I CACGTG 1 cut(s) 9620
Eco88I CYCGRG 4 cut(s) 197, 2638, 5114, 9829
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 3 cut(s) 2554, 3314, 7298
EcoRI GAATTC 2 cut(s) 6456, 8834
EcoRV GATATC 2 cut(s) 3000, 7138
EcoT22I ATGCAT 3 cut(s) 1572, 4058, 5966
EcoT38I GRGCYC 2 cut(s) 198, 10815
Esp3I CGTCTC 1 cut(s) 8499
FauI CCCGC 3 cut(s) 125, 698, 7495
FauNDI CATATG 3 cut(s) 3307, 3558, 9855
FblI GTMKAC 7 cut(s) 2097, 2860, 4229, 4428, 6240, 7961, 10627
FriOI GRGCYC 2 cut(s) 198, 10815
GlaI GCGC 6 cut(s) 1696, 3216, 9322, 9624, 9626, 9910
GsaI CCCAGC 2 cut(s) 5831, 10514
GsuI CTGGAG 8 cut(s) 3128, 3528, 4167, 5403, 6699, 8103, 9216, 9474
HaeII RGCGCY 1 cut(s) 1698
HgaI GACGC 4 cut(s) 902, 1196, 6066, 10363
HhaI GCGC 6 cut(s) 1697, 3217, 9323, 9625, 9627, 9911
Hin1I GRCGYC 4 cut(s) 4995, 8508, 8715, 10374
Hin6I GCGC 6 cut(s) 1695, 3215, 9321, 9623, 9625, 9909
HinP1I GCGC 6 cut(s) 1695, 3215, 9321, 9623, 9625, 9909
HincII GTYRAC 5 cut(s) 483, 2019, 4652, 4720, 10481
HindII GTYRAC 5 cut(s) 483, 2019, 4652, 4720, 10481
HindIII AAGCTT 7 cut(s) 1282, 1846, 4601, 6407, 6539, 10488, 10708
Hpy99I CGWCG 6 cut(s) 733, 2868, 3996, 5847, 8031, 10479
Hsp92I GRCGYC 4 cut(s) 4995, 8508, 8715, 10374
HspAI GCGC 6 cut(s) 1695, 3215, 9321, 9623, 9625, 9909
KroI GCCGGC 1 cut(s) 3737
KroNI GCCGGC 1 cut(s) 3739
LguI GCTCTTC 3 cut(s) 186, 1127, 6045
MbiI CCGCTC 1 cut(s) 8339
MfeI CAATTG 7 cut(s) 234, 603, 1003, 1545, 3932, 5783, 10213
MflI RGATCY 9 cut(s) 1298, 2195, 2665, 3371, 4416, 4483, 5188, 8167, 9711
MlsI TGGCCA 3 cut(s) 1593, 7186, 9115
MluNI TGGCCA 3 cut(s) 1593, 7186, 9115
MmeI TCCRAC 9 cut(s) 507, 778, 840, 1155, 3349, 3840, 4887, 7771, 10286
Mox20I TGGCCA 3 cut(s) 1593, 7186, 9115
Mph1103I ATGCAT 3 cut(s) 1572, 4058, 5966
MroNI GCCGGC 1 cut(s) 3737
MscI TGGCCA 3 cut(s) 1593, 7186, 9115
MslI CAYNNNNRTG 7 cut(s) 2602, 3363, 6362, 7197, 7727, 9126, 10677
Msp20I TGGCCA 3 cut(s) 1593, 7186, 9115
MspA1I CMGCKG 4 cut(s) 3646, 5461, 6083, 7564
MspCI CTTAAG 3 cut(s) 1379, 1714, 6536
MssI GTTTAAAC 1 cut(s) 5896
MunI CAATTG 7 cut(s) 234, 603, 1003, 1545, 3932, 5783, 10213
Mva1269I GAATGC 4 cut(s) 329, 596, 5966, 8984
MvnI CGCG 3 cut(s) 852, 8053, 9625
NaeI GCCGGC 1 cut(s) 3739
NciI CCSGG 3 cut(s) 3119, 9830, 9831
NcoI CCATGG 5 cut(s) 9, 2610, 7805, 10461, 10729
NdeI CATATG 3 cut(s) 3307, 3558, 9855
NgoMIV GCCGGC 1 cut(s) 3737
NheI GCTAGC 1 cut(s) 973
NmeAIII GCCGAG 1 cut(s) 3236
NruI TCGCGA 1 cut(s) 852
NsiI ATGCAT 3 cut(s) 1572, 4058, 5966
NspV TTCGAA 2 cut(s) 751, 1609
PaeI GCATGC 3 cut(s) 1440, 6232, 8001
PaeR7I CTCGAG 1 cut(s) 197
PagI TCATGA 4 cut(s) 1651, 3319, 3358, 10672
PauI GCGCGC 1 cut(s) 9623
PciI ACATGT 6 cut(s) 154, 3658, 5473, 5680, 7411, 7579
PciSI GCTCTTC 3 cut(s) 186, 1127, 6045
PcsI WCGNNNNNNNCGW 2 cut(s) 3436, 9910
PctI GAATGC 4 cut(s) 329, 596, 5966, 8984
PdiI GCCGGC 1 cut(s) 3739
PflMI CCANNNNNTGG 3 cut(s) 5248, 7033, 7193
PmaCI CACGTG 1 cut(s) 9620
PmeI GTTTAAAC 1 cut(s) 5896
PmlI CACGTG 1 cut(s) 9620
Ppu21I YACGTR 6 cut(s) 1403, 5037, 6952, 8082, 9620, 10173
PpuMI RGGWCCY 2 cut(s) 2554, 3314
PscI ACATGT 6 cut(s) 154, 3658, 5473, 5680, 7411, 7579
PshBI ATTAAT 3 cut(s) 998, 4782, 9417
PsiI TTATAA 4 cut(s) 875, 1034, 3057, 10281
Psp124BI GAGCTC 1 cut(s) 198
Psp1406I AACGTT 1 cut(s) 6553
Psp5II RGGWCCY 2 cut(s) 2554, 3314
PspCI CACGTG 1 cut(s) 9620
PspFI CCCAGC 2 cut(s) 5827, 10510
PspPI GGNCC 8 cut(s) 1996, 2554, 3314, 3679, 5503, 7208, 7298, 8146
PspPPI RGGWCCY 2 cut(s) 2554, 3314
PsrI GAACNNNNNNTAC 2 cut(s) 10047, 10079
PstI CTGCAG 5 cut(s) 2189, 4495, 7501, 7972, 8552
PstNI CAGNNNCTG 1 cut(s) 6466
PsuI RGATCY 9 cut(s) 1298, 2195, 2665, 3371, 4416, 4483, 5188, 8167, 9711
PteI GCGCGC 1 cut(s) 9623
PvuII CAGCTG 3 cut(s) 3646, 5461, 7564
RruI TCGCGA 1 cut(s) 852
RseI CAYNNNNRTG 7 cut(s) 2602, 3363, 6362, 7197, 7727, 9126, 10677
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 3 cut(s) 186, 1127, 6045
Sau96I GGNCC 8 cut(s) 1996, 2554, 3314, 3679, 5503, 7208, 7298, 8146
ScaI AGTACT 3 cut(s) 5593, 7845, 9190
SfcI CTRYAG 7 cut(s) 2185, 2937, 3852, 4491, 7497, 7968, 8548
Sfr274I CTCGAG 1 cut(s) 197
SfuI TTCGAA 2 cut(s) 751, 1609
SinI GGWCC 6 cut(s) 1996, 2554, 3314, 5503, 7208, 8146
SlaI CTCGAG 1 cut(s) 197
SmaI CCCGGG 1 cut(s) 9831
SmiMI CAYNNNNRTG 7 cut(s) 2602, 3363, 6362, 7197, 7727, 9126, 10677
SnaBI TACGTA 1 cut(s) 10173
SpeI ACTAGT 6 cut(s) 4232, 5438, 7373, 7541, 7570, 7840
SphI GCATGC 3 cut(s) 1440, 6232, 8001
SspI AATATT 1 cut(s) 5613
SstI GAGCTC 1 cut(s) 198
TaqII GACCGA 2 cut(s) 965, 3101
TauI GCSGC 3 cut(s) 2596, 3022, 4271
TspGWI ACGGA 8 cut(s) 2893, 3533, 4409, 7046, 7946, 9257, 9704, 10042
TspMI CCCGGG 1 cut(s) 9829
Van91I CCANNNNNTGG 3 cut(s) 5248, 7033, 7193
Vha464I CTTAAG 3 cut(s) 1379, 1714, 6536
VneI GTGCAC 2 cut(s) 5654, 8055
VpaK11BI GGWCC 6 cut(s) 1996, 2554, 3314, 5503, 7208, 8146
VspI ATTAAT 3 cut(s) 998, 4782, 9417
XbaI TCTAGA 3 cut(s) 3154, 4120, 5938
XcmI CCANNNNNNNNNTGG 3 cut(s) 5458, 7393, 9335
XhoI CTCGAG 1 cut(s) 197
XmaI CCCGGG 1 cut(s) 9829
XmaJI CCTAGG 1 cut(s) 8521
XmiI GTMKAC 7 cut(s) 2097, 2860, 4229, 4428, 6240, 7961, 10627
ZraI GACGTC 3 cut(s) 4996, 8509, 8716
ZrmI AGTACT 3 cut(s) 5593, 7845, 9190
Zsp2I ATGCAT 3 cut(s) 1572, 4058, 5966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.