Rroxscaffold_5G00371430

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016427
Physical Location & Seq
Forward (+)
52989050 .. 53000226
11177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371430.1

Sequence Viewer

Length: 3870 bp
ATGGAAAAGAGAGAAACCATAGGAGATTTCGCTAGAGCCCCGTCACCGGTAGCGAGGATTCCGGCCAACTTTCACCGGATCCCGATCAACGACCGTCGTATTCCAGTCACCTATCGCCGGATTCCGGTCACGGACTTTTTGAAAACCTCGCCGGAGGTTTTCAAAGAGGTCGTCGGAGATTTCATAGACTGCTTGAAATTGACAGCAGCTTCATATACCAAAACTAAAGCTGGAACAAATGTCCCCAAGAAGCCAAACATCCTCGGAACCAACAACTTAATTGAGAGAGAGGTCATCAAAGAAATAGTACAACGTGTTTATAGTTATTTGAATCTCCAATCGAATACCGCTTTCTCCACTGCTGATCTCAAAGAGTTTGTTGGTATGCATCGTGTAGAGGTAATTGAAGCGGACCTACTCATTGACCAGTGGGATGAAGTTCGTGTTATTGGCATTTGCGGGATGCCGGGAATAGGTAAAACGACCATTGCACGAGTTATTTTTGAAAAGATTCGCACAAAATTTGAAGCAAGCAGTTTCGTTGATAATGTTGGTGATGTATATGAAAAGAAAAGCTTACTGCATATACAAAAACAACTTTATAAGGAGTTGTTGCACAGCGAGGTAGACAGCGGGAACGTCAATGAAGTAAGGGGTAGATTGCATAACAGAAGGGTGCTTCTCATTCTTGATAGCGTTGATGACTTGATTCAAATAAAGCACCTAGCTGGAAAGGGTGGTGAAGAACTAAAGGATTGGTTTGGTAAAGGTAGTAGAATCCTCATAACCACTAGAGATGAAAAGCTGTTGACGGATTATGGGGTAGATAATATACACAAGATTGAGACACTTACTGAAGATGAGGCTCTCCTGCTTTTCCGTCTGAATGCCTTCAAGAAAGACACTCCTCAGGATGATAAAGTTGGGTTGTGTTACAAATTTATAGATTACGCGGACGGACTTCCTTTGGCTCTTGAAGTTTTCGGTTCCTTTTTATCGAACAGATCCGTAAAAGAATGGGAACTTGAATATGCACAACTGAAAGCTAATAATTATTGTGGTGAAGAAAAACTTTTCGGTATTCTTAAAGCAAGCATTAATGGATTACGTAGTGGAGACAAGCAAAACTTCCTGGACACTGCATGTTTCTTCAGAGGATACGATGTAAATCGTGTAGAGAAGATATTTGAAAGGAGTGGCAACGTAAACATCCTTCTTGAGAGATCTCTTGTAACTATTGTAGGAGGAAAATTGTGGATGCACGATTTATTACAAGAAACTGGCCGGAGAATGGTTCGCGAGGAATCCGAAGAGTTGGGGAAACGCAGCAGGCTGTGGCTTCATAGAGAGGCCCAAACTGTACTCGAGTCTAATAAGATCACCTTAAATATGCTGGTGACAATGGACCTTAGTGATTGCAAAGACTTGACCAAGACCCCTGACTTCAAAGCAGTCCGTAAGCTTGAGAAGCTAATCCTCAAAGGTTGCAAAAGATTGTCTGAAGTTGACTCATCAATTTTGGGTCTCCAATGGCTCCATTTGTTGAATCTGGAAGGTTGTGAATCCTTGGTTAATCTTCCCCGACGTATCAACCTAAGATCTCTCATAACTCTCATTCTTTCGGGATGTTCCAAATTTCAGAAGTTTCCAGAAATTGGAAAGGGGATGGAAAAATTATCGGAACTTCGTTTACGTGGGACGGCTATAGATGCGTTACCGGCATCAATCAAGAATTTGACTGGCCTTTCTCTGCTCGATCTAGCAGACTGCAAGGAGCTTTCGAGTCTTCCGAATGACATATGCACCTTGATGTCACTTCGATGTATGACCCTTGCAGGCTGCTCAAATCTTCACGAGTTGCCTGAAAATTTTGGGAGCTTAGAAGGTTTGGAGCTATTCAGTATAAGATATTCTGGTATAACACGAGCACCTTCCTCCATTCAATGTCTGAAAAACCTCAAACAACTGGAAATCTATGGATGTGGTAGTGGTTCGTCCTCCAATTCCATTGGTTTTCAGTTGCCCAATTCGTTGTTGGGATTATGCTCTTTGGAGATTTTAGATATGAGAAAATGCAATCTTTCTGAAGGAGCAATCCCCGATGATATTGGCCGTTTGTCCTCACTGAAGCAATTATATTTAAGCAGAAACAATTTTGTGACGGTACCCAGCAGCATCTCTGAGCTTCCTCAGCTTGAAACCCTTCTATTGGATAATTGCAGTAACCTGAAGTCATTGCCGGAACTTCCTCCAAACATACGAACTGTATTTGCAAGGAATTGCCCTATCTTGCAGGGACCATATGCAAATAAATTGGTGAAATACCCAAACTTCAGAGGGTTCCGATATCAGAACAGAATCGTAAACCTCTACACGATCAACAGTCTTATGAGGTGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACACACACACACACTAACCATGTTTGTTTCTAACTCGATCTTCTGCAATTTAATTCAGGATAAGATTCGACGCGGTGGTGGTTTTGGATATGTTAGTTGTCCTCACACTGAAATTCCATCATGGTTCCGTCGTCAGAGTAAGGGAGAGTCAATAAGAATCCAGCTACCTCCAGATCTCGATGGTGAAAAGCAATGGATTGGATTTGCCCTCTGTTTTCTGACTTTTCTCTATGAATCGGAGGTGCATGAACAAATAAATCCAGACGAATTAACATACAGTTGCAATTACCGTTGTTATATCTACACAAATGAAGATGATGTTCATGAAATTCCCCTACAGTGTAGACTGATGGACAACAATAAAGGTGGCCCAAATCGACATTGGCACTACGCACCGAGACATGTGTTTGCAAACAGGTTGATGAGACGCTCCATCCGAGTTGAAATTAGAACCGACAGAGCAGCGGTGAAGGTGACAGCGTGTGGGGCAAGACTGCTGTACAATGACGACGTACAAGAATTTGTCCATAATATGATTGCATGGGAGTCCGATCTTAAAACTCCTCCTGACGTACTTGTACTAAGAAGGCTGACAGTTCCTTCTGATGAACCAGAAGAAGAATTCGGTTGCGCTGCAGAAACAAGATTCTTGATTGAAATTGAGCCACGAGGTTCGGGGAATCATTCAAGTTCGTCTCAGAATGTAAGTGAAGTTAGTGAACCATTGATCAGGAAAAAATTAGAGTCACTGCTTGAACAATTATTCGAGTCGGGGGGCAACGCAAGACGCTTAGATTGCGGATTTGTTTTCTGTCTCCGAGCGATTTTGCCGTGGTTTAATAACCAAAGCATTGGGAGCATAGTCGATCCAGAAACCACCGTAAGTGCTAGCGAAGTAGCCGTGACAATTCCTGAAAATCTGTATAATGATAAGGAATGGGTAGGATTTGCTCTATGTGTGTCCTTCACTCTGCCTCCAGATGCTTTGCTTCCAAAAAATTCAGTGTTCAAGTCAAGCTTGTCCACCGCTGACGGAGTTGGGTATCGCCTGGGATTAGTATCAGCCGGTGAAGACAGTTTTATGGGGTCACATCGACTGCTTGTTATTCACATCCCGCGAGTACGTTTTACAGAACAGTTGAATCAACTCCGAGCCATTTATGCTTCTTTTGGCTCCAATTGTTTTGGTGTGGAGGTTGAAATGTGTGGGATGCGAGTAGTATATGAAAGAGATTTGATAGGGTTAATCCAAACAATTAGCCACTGCACCGCAATTAGTAGTCCGCCAGGTTACTACGGACTTGCTCGTGAGGAACAATTTATGACACAGAAGGGTCAGCCTTTCAGATTGTCGATTATGGACAAGAGTTTGCTTGAAGCAGCTGAATCCAGCTCGAGGTTAAAATCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1289

Amino Acids

145.55

Weight (kDa)

7.12

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 144 - 301 8.4e-19 NB-ARC domain
NACHT PF05729 148 - 294 9e-10 NACHT domain
WHD_ROQ1 PF23282 373 - 436 1.8e-12 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 532 - 619 3.2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 561 - 693 9e-15 Leucine-rich repeat region
LRR_13 PF23286 617 - 749 4.5e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 852 - 991 1.3e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
Acc65I GGTACC 1 cut(s) 2164
AccB1I GGYRCC 1 cut(s) 2164
AccB7I CCANNNNNTGG 1 cut(s) 1655
AccI GTMKAC 2 cut(s) 627, 2800
AccII CGCG 4 cut(s) 953, 1299, 2529, 3574
AclWI GGATC 4 cut(s) 73, 86, 999, 3317
AcoI YGGCCR 3 cut(s) 63, 1282, 2110
AcuI CTGAAG 7 cut(s) 876, 1135, 1521, 2106, 2147, 2249, 2317
AfaI GTAC 8 cut(s) 309, 1362, 2166, 2957, 2970, 3030, 3036, 3579
AfiI CCNNNNNNNGG 8 cut(s) 46, 117, 124, 473, 1291, 1655, 2209, 3852
AflIII ACRYGT 2 cut(s) 313, 2857
AgeI ACCGGT 1 cut(s) 46
AjnI CCWGG 3 cut(s) 1131, 3504, 3742
Alw21I GWGCWC 1 cut(s) 1930
Alw26I GTCTC 8 cut(s) 839, 1110, 1529, 2402, 2848, 2875, 3156, 3275
AlwI GGATC 4 cut(s) 73, 86, 999, 3317
Ama87I CYCGRG 2 cut(s) 1364, 3850
AoxI GGCC 6 cut(s) 63, 1282, 1350, 1741, 2110, 2824
ApeKI GCWGC 7 cut(s) 206, 1326, 1839, 2172, 2918, 3089, 3836
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AseI ATTAAT 1 cut(s) 1098
AsiGI ACCGGT 1 cut(s) 46
Asp700I GAANNNNTTC 3 cut(s) 510, 890, 2202
Asp718I GGTACC 1 cut(s) 2164
AspLEI GCGC 1 cut(s) 3089
AspS9I GGNCC 5 cut(s) 412, 1351, 1405, 2297, 2825
AsuC2I CCSGG 1 cut(s) 468
AsuNHI GCTAGC 1 cut(s) 3344
AvaI CYCGRG 2 cut(s) 1364, 3850
AvaII GGWCC 3 cut(s) 412, 1405, 2297
AxyI CCTNAGG 1 cut(s) 909
BamHI GGATCC 1 cut(s) 78
BanI GGYRCC 1 cut(s) 2164
BanII GRGCYC 1 cut(s) 40
BarI GAAGNNNNNNTAC 4 cut(s) 399, 431, 1197, 1229
BauI CACGAG 5 cut(s) 492, 1853, 1923, 3123, 3762
BbsI GAAGAC 2 cut(s) 1778, 3534
Bbv12I GWGCWC 1 cut(s) 1930
BbvCI CCTCAGC 1 cut(s) 2190
BbvI GCAGC 7 cut(s) 218, 1338, 1826, 2184, 2930, 3076, 3848
BccI CCATC 5 cut(s) 1660, 2581, 2630, 2800, 2897
BceAI ACGGC 4 cut(s) 1716, 2097, 3271, 3341
BciT130I CCWGG 3 cut(s) 1133, 3506, 3744
BciVI GTATCC 1 cut(s) 1151
BclI TGATCA 1 cut(s) 3183
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 8 cut(s) 839, 1110, 1529, 2402, 2848, 2875, 3156, 3275
BfaI CTAG 5 cut(s) 33, 725, 792, 1760, 3345
BfmI CTRYAG 3 cut(s) 1704, 2792, 3090
BfuI GTATCC 1 cut(s) 1151
BglII AGATCT 3 cut(s) 1223, 1598, 2629
BisI GCNGC 7 cut(s) 207, 1327, 1840, 2173, 2919, 3090, 3837
BlsI GCNGC 7 cut(s) 208, 1328, 1841, 2174, 2920, 3091, 3838
Bme1390I CCNGG 4 cut(s) 468, 1133, 3506, 3744
Bme18I GGWCC 3 cut(s) 412, 1405, 2297
BmeT110I CYCGRG 2 cut(s) 1364, 3850
BmgT120I GGNCC 5 cut(s) 412, 1351, 1405, 2297, 2825
BmiI GGNNCC 9 cut(s) 80, 268, 988, 1535, 2166, 2298, 2342, 2582, 3631
BmrFI CCNGG 4 cut(s) 468, 1133, 3506, 3744
BmsI GCATC 8 cut(s) 397, 453, 1248, 1699, 1730, 2184, 3427, 3657
BmtI GCTAGC 1 cut(s) 3348
BpiI GAAGAC 2 cut(s) 1778, 3534
BpmI CTGGAG 2 cut(s) 2610, 3417
Bpu10I CCTNAGC 1 cut(s) 2190
BpuEI CTTGAG 2 cut(s) 1238, 1484
BpuMI CCSGG 1 cut(s) 468
BsaAI YACGTR 2 cut(s) 1109, 1694
BsaBI GATNNNNATC 3 cut(s) 83, 1167, 3514
BsaI GGTCTC 1 cut(s) 1529
BsaJI CCNNGG 4 cut(s) 262, 1566, 3287, 3505
BsaWI WCCGGW 3 cut(s) 46, 75, 124
BsaXI ACNNNNNCTCC 2 cut(s) 3415, 3445
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 117, 124, 473, 1291, 1655, 2209, 3852
Bse118I RCCGGY 3 cut(s) 46, 1717, 3521
Bse1I ACTGG 5 cut(s) 104, 427, 1285, 1744, 1971
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 3 cut(s) 486, 2234, 2654
Bse8I GATNNNNATC 3 cut(s) 83, 1167, 3514
BseBI CCWGG 3 cut(s) 1133, 3506, 3744
BseDI CCNNGG 4 cut(s) 262, 1566, 3287, 3505
BseJI GATNNNNATC 3 cut(s) 83, 1167, 3514
BseLI CCNNNNNNNGG 8 cut(s) 46, 117, 124, 473, 1291, 1655, 2209, 3852
BseMI GCAATG 3 cut(s) 486, 2234, 2654
BseMII CTCAG 4 cut(s) 923, 2172, 2204, 3167
BseNI ACTGG 5 cut(s) 104, 427, 1285, 1744, 1971
BseRI GAGGAG 2 cut(s) 897, 3009
BseXI GCAGC 7 cut(s) 218, 1338, 1826, 2184, 2930, 3076, 3848
BseYI CCCAGC 1 cut(s) 2168
BsgI GTGCAG 1 cut(s) 3706
Bsh1236I CGCG 4 cut(s) 953, 1299, 2529, 3574
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 6 cut(s) 65, 1284, 1352, 1743, 2112, 2826
BshNI GGYRCC 1 cut(s) 2164
BshTI ACCGGT 1 cut(s) 46
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 1930
BsiHKCI CYCGRG 2 cut(s) 1364, 3850
BslFI GGGAC 3 cut(s) 227, 1711, 2310
BslI CCNNNNNNNGG 8 cut(s) 46, 117, 124, 473, 1291, 1655, 2209, 3852
BsmAI GTCTC 8 cut(s) 839, 1110, 1529, 2402, 2848, 2875, 3156, 3275
BsmBI CGTCTC 2 cut(s) 2875, 3156
BsmFI GGGAC 3 cut(s) 227, 1711, 2310
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 6 cut(s) 65, 1284, 1352, 1743, 2112, 2826
Bso31I GGTCTC 1 cut(s) 1529
BsoBI CYCGRG 2 cut(s) 1364, 3850
Bsp1286I GDGCHC 2 cut(s) 40, 1930
Bsp1407I TGTACA 1 cut(s) 2955
Bsp68I TCGCGA 1 cut(s) 1299
BspANI GGCC 6 cut(s) 65, 1284, 1352, 1743, 2112, 2826
BspCNI CTCAG 4 cut(s) 922, 2173, 2203, 3166
BspFNI CGCG 4 cut(s) 953, 1299, 2529, 3574
BspHI TCATGA 1 cut(s) 2779
BspLI GGNNCC 9 cut(s) 80, 268, 988, 1535, 2166, 2298, 2342, 2582, 3631
BspMAI CTGCAG 1 cut(s) 3094
BspOI GCTAGC 1 cut(s) 3348
BspPI GGATC 4 cut(s) 73, 86, 999, 3317
BspT107I GGYRCC 1 cut(s) 2164
BspTNI GGTCTC 1 cut(s) 1529
BsrDI GCAATG 3 cut(s) 486, 2234, 2654
BsrFI RCCGGY 3 cut(s) 46, 1717, 3521
BsrGI TGTACA 1 cut(s) 2955
BsrI ACTGG 5 cut(s) 104, 427, 1285, 1744, 1971
BssAI RCCGGY 3 cut(s) 46, 1717, 3521
BssECI CCNNGG 4 cut(s) 262, 1566, 3287, 3505
BssSI CACGAG 5 cut(s) 492, 1853, 1923, 3123, 3762
BssT1I CCWWGG 1 cut(s) 1566
Bst2BI CACGAG 5 cut(s) 492, 1853, 1923, 3123, 3762
Bst2UI CCWGG 3 cut(s) 1133, 3506, 3744
Bst6I CTCTTC 1 cut(s) 1305
BstAUI TGTACA 1 cut(s) 2955
BstBAI YACGTR 2 cut(s) 1109, 1694
BstC8I GCNNGC 5 cut(s) 532, 1093, 1331, 1837, 3346
BstDEI CTNAG 9 cut(s) 909, 1409, 1595, 1879, 2181, 2190, 3038, 3153, 3247
BstDSI CCRYGG 1 cut(s) 3287
BstFNI CGCG 4 cut(s) 953, 1299, 2529, 3574
BstHHI GCGC 1 cut(s) 3089
BstMAI GTCTC 8 cut(s) 839, 1110, 1529, 2402, 2848, 2875, 3156, 3275
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 8 cut(s) 1468, 1709, 1718, 2191, 2942, 3252, 3312, 3617
BstNI CCWGG 3 cut(s) 1133, 3506, 3744
BstNSI RCATGY 2 cut(s) 1146, 2861
BstSCI CCNGG 4 cut(s) 466, 1131, 3504, 3742
BstSFI CTRYAG 3 cut(s) 1704, 2792, 3090
BstSNI TACGTA 1 cut(s) 1109
BstUI CGCG 4 cut(s) 953, 1299, 2529, 3574
BstV1I GCAGC 7 cut(s) 218, 1338, 1826, 2184, 2930, 3076, 3848
BstV2I GAAGAC 2 cut(s) 1778, 3534
BstX2I RGATCY 5 cut(s) 78, 1004, 1223, 1598, 2629
BstXI CCANNNNNNTGG 1 cut(s) 3308
BstYI RGATCY 5 cut(s) 78, 1004, 1223, 1598, 2629
Bsu36I CCTNAGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 1151
BsuRI GGCC 6 cut(s) 65, 1284, 1352, 1743, 2112, 2826
BtgI CCRYGG 1 cut(s) 3287
BtsI GCAGTG 4 cut(s) 357, 1137, 3203, 3718
BtsIMutI CAGTG 9 cut(s) 357, 434, 1137, 2123, 2562, 2801, 3203, 3465, 3718
BtuMI TCGCGA 1 cut(s) 1299
Cac8I GCNNGC 5 cut(s) 532, 1093, 1331, 1837, 3346
CciI TCATGA 1 cut(s) 2779
CfoI GCGC 1 cut(s) 3089
Cfr10I RCCGGY 3 cut(s) 46, 1717, 3521
Cfr13I GGNCC 5 cut(s) 412, 1351, 1405, 2297, 2825
CseI GACGC 3 cut(s) 2535, 2892, 3252
Csp6I GTAC 8 cut(s) 308, 1361, 2165, 2956, 2969, 3029, 3035, 3578
CspAI ACCGGT 1 cut(s) 46
CspCI CAANNNNNGTGG 2 cut(s) 2803, 2838
CviAII CATG 7 cut(s) 1143, 2476, 2577, 2702, 2780, 2858, 2997
CviQI GTAC 8 cut(s) 308, 1361, 2165, 2956, 2969, 3029, 3035, 3578
DdeI CTNAG 9 cut(s) 909, 1409, 1595, 1879, 2181, 2190, 3038, 3153, 3247
EaeI YGGCCR 3 cut(s) 63, 1282, 2110
Eam1104I CTCTTC 1 cut(s) 1305
EarI CTCTTC 1 cut(s) 1305
EciI GGCGGA 1 cut(s) 3729
Eco105I TACGTA 1 cut(s) 1109
Eco130I CCWWGG 1 cut(s) 1566
Eco24I GRGCYC 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 1529
Eco32I GATATC 1 cut(s) 2348
Eco47I GGWCC 3 cut(s) 412, 1405, 2297
Eco57I CTGAAG 7 cut(s) 876, 1135, 1521, 2106, 2147, 2249, 2317
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 2 cut(s) 1364, 3850
EcoRI GAATTC 1 cut(s) 3077
EcoRII CCWGG 3 cut(s) 1131, 3504, 3742
EcoRV GATATC 1 cut(s) 2348
EcoT14I CCWWGG 1 cut(s) 1566
EcoT22I ATGCAT 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 1566
Esp3I CGTCTC 2 cut(s) 2875, 3156
FaeI CATG 7 cut(s) 1146, 2479, 2580, 2705, 2783, 2861, 3000
FaqI GGGAC 3 cut(s) 227, 1711, 2310
FatI CATG 7 cut(s) 1142, 2475, 2576, 2701, 2779, 2857, 2996
FauI CCCGC 3 cut(s) 452, 626, 3579
FauNDI CATATG 2 cut(s) 1799, 2302
FbaI TGATCA 1 cut(s) 3183
FblI GTMKAC 2 cut(s) 627, 2800
Fnu4HI GCNGC 7 cut(s) 207, 1327, 1840, 2173, 2919, 3090, 3837
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 7 cut(s) 207, 1327, 1840, 2173, 2919, 3090, 3837
FspBI CTAG 5 cut(s) 33, 725, 792, 1760, 3345
GlaI GCGC 1 cut(s) 3088
GluI GCNGC 7 cut(s) 207, 1327, 1840, 2173, 2919, 3090, 3837
GsaI CCCAGC 1 cut(s) 2172
GsuI CTGGAG 2 cut(s) 2610, 3417
HaeIII GGCC 6 cut(s) 65, 1284, 1352, 1743, 2112, 2826
HgaI GACGC 3 cut(s) 2535, 2892, 3252
HhaI GCGC 1 cut(s) 3089
Hin1II CATG 7 cut(s) 1146, 2479, 2580, 2705, 2783, 2861, 3000
Hin6I GCGC 1 cut(s) 3087
HinP1I GCGC 1 cut(s) 3087
HincII GTYRAC 2 cut(s) 810, 1507
HindII GTYRAC 2 cut(s) 810, 1507
HindIII AAGCTT 3 cut(s) 574, 1460, 3472
Hpy166II GTNNAC 9 cut(s) 628, 810, 1207, 1507, 1691, 2365, 2801, 3176, 3480
Hpy8I GTNNAC 9 cut(s) 628, 810, 1207, 1507, 1691, 2365, 2801, 3176, 3480
Hpy99I CGWCG 6 cut(s) 99, 176, 1587, 2529, 2589, 2969
HpyCH4IV ACGT 9 cut(s) 313, 639, 1108, 1203, 1585, 1693, 2967, 3027, 3580
HpyF10VI GCNNNNNNNGC 8 cut(s) 1468, 1709, 1718, 2191, 2942, 3252, 3312, 3617
HpyF3I CTNAG 9 cut(s) 909, 1409, 1595, 1879, 2181, 2190, 3038, 3153, 3247
HpySE526I ACGT 9 cut(s) 313, 639, 1108, 1203, 1585, 1693, 2967, 3027, 3580
Hsp92II CATG 7 cut(s) 1146, 2479, 2580, 2705, 2783, 2861, 3000
HspAI GCGC 1 cut(s) 3087
KpnI GGTACC 1 cut(s) 2168
Ksp22I TGATCA 1 cut(s) 3183
LmnI GCTCC 8 cut(s) 1539, 1774, 1875, 1891, 2090, 2891, 3312, 3635
Lsp1109I GCAGC 7 cut(s) 218, 1338, 1826, 2184, 2930, 3076, 3848
LweI GCATC 8 cut(s) 397, 453, 1248, 1699, 1730, 2184, 3427, 3657
MaeI CTAG 5 cut(s) 33, 725, 792, 1760, 3345
MaeII ACGT 9 cut(s) 313, 639, 1108, 1203, 1585, 1693, 2967, 3027, 3580
MfeI CAATTG 1 cut(s) 3634
MflI RGATCY 5 cut(s) 78, 1004, 1223, 1598, 2629
MhlI GDGCHC 2 cut(s) 40, 1930
MlyI GAGTC 7 cut(s) 1376, 1502, 1792, 2612, 3011, 3209, 3233
MmeI TCCRAC 1 cut(s) 154
Mph1103I ATGCAT 1 cut(s) 390
MroXI GAANNNNTTC 3 cut(s) 510, 890, 2202
MslI CAYNNNNRTG 2 cut(s) 1808, 1819
MspA1I CMGCKG 4 cut(s) 633, 2921, 3485, 3839
MspR9I CCNGG 4 cut(s) 468, 1133, 3506, 3744
MunI CAATTG 1 cut(s) 3634
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 3 cut(s) 1133, 3506, 3744
MvnI CGCG 4 cut(s) 953, 1299, 2529, 3574
MwoI GCNNNNNNNGC 8 cut(s) 1468, 1709, 1718, 2191, 2942, 3252, 3312, 3617
NciI CCSGG 1 cut(s) 468
NdeI CATATG 2 cut(s) 1799, 2302
NheI GCTAGC 1 cut(s) 3344
NlaIII CATG 7 cut(s) 1146, 2479, 2580, 2705, 2783, 2861, 3000
NlaIV GGNNCC 9 cut(s) 80, 268, 988, 1535, 2166, 2298, 2342, 2582, 3631
NruI TCGCGA 1 cut(s) 1299
NsiI ATGCAT 1 cut(s) 390
NspI RCATGY 2 cut(s) 1146, 2861
PaeR7I CTCGAG 2 cut(s) 1364, 3850
PagI TCATGA 1 cut(s) 2779
PciI ACATGT 1 cut(s) 2857
PcsI WCGNNNNNNNCGW 2 cut(s) 1787, 2890
PctI GAATGC 1 cut(s) 892
PdmI GAANNNNTTC 3 cut(s) 510, 890, 2202
PflMI CCANNNNNTGG 1 cut(s) 1655
PfoI TCCNGGA 1 cut(s) 1131
PinAI ACCGGT 1 cut(s) 46
PkrI GCNGC 7 cut(s) 208, 1328, 1841, 2174, 2920, 3091, 3838
PleI GAGTC 7 cut(s) 1375, 1502, 1791, 2611, 3010, 3208, 3232
PpsI GAGTC 7 cut(s) 1375, 1502, 1791, 2611, 3010, 3208, 3232
Ppu21I YACGTR 2 cut(s) 1109, 1694
PscI ACATGT 1 cut(s) 2857
PshBI ATTAAT 1 cut(s) 1098
PsiI TTATAA 1 cut(s) 603
Psp6I CCWGG 3 cut(s) 1131, 3504, 3742
PspFI CCCAGC 1 cut(s) 2168
PspGI CCWGG 3 cut(s) 1131, 3504, 3742
PspN4I GGNNCC 9 cut(s) 80, 268, 988, 1535, 2166, 2298, 2342, 2582, 3631
PspPI GGNCC 5 cut(s) 412, 1351, 1405, 2297, 2825
PspXI VCTCGAGB 2 cut(s) 1364, 3850
PsrI GAACNNNNNNTAC 2 cut(s) 1674, 1706
PstI CTGCAG 1 cut(s) 3094
PsuI RGATCY 5 cut(s) 78, 1004, 1223, 1598, 2629
PvuII CAGCTG 1 cut(s) 3839
RruI TCGCGA 1 cut(s) 1299
RsaI GTAC 8 cut(s) 309, 1362, 2166, 2957, 2970, 3030, 3036, 3579
RsaNI GTAC 8 cut(s) 308, 1361, 2165, 2956, 2969, 3029, 3035, 3578
RseI CAYNNNNRTG 2 cut(s) 1808, 1819
SatI GCNGC 7 cut(s) 207, 1327, 1840, 2173, 2919, 3090, 3837
Sau96I GGNCC 5 cut(s) 412, 1351, 1405, 2297, 2825
SchI GAGTC 7 cut(s) 1376, 1502, 1792, 2612, 3011, 3209, 3233
ScrFI CCNGG 4 cut(s) 468, 1133, 3506, 3744
SduI GDGCHC 2 cut(s) 40, 1930
SfaNI GCATC 8 cut(s) 397, 453, 1248, 1699, 1730, 2184, 3427, 3657
SfcI CTRYAG 3 cut(s) 1704, 2792, 3090
Sfr274I CTCGAG 2 cut(s) 1364, 3850
SinI GGWCC 3 cut(s) 412, 1405, 2297
SlaI CTCGAG 2 cut(s) 1364, 3850
SmiMI CAYNNNNRTG 2 cut(s) 1808, 1819
SmlI CTYRAG 4 cut(s) 1217, 1364, 1463, 3850
SmoI CTYRAG 4 cut(s) 1217, 1364, 1463, 3850
SnaBI TACGTA 1 cut(s) 1109
SspMI CTAG 5 cut(s) 33, 725, 792, 1760, 3345
StyD4I CCNGG 4 cut(s) 466, 1131, 3504, 3742
StyI CCWWGG 1 cut(s) 1566
TaiI ACGT 9 cut(s) 316, 642, 1111, 1206, 1588, 1696, 2970, 3030, 3583
TatI WGTACW 4 cut(s) 307, 1360, 2955, 3034
TscAI CASTG 9 cut(s) 364, 434, 1144, 2130, 2569, 2801, 3210, 3465, 3725
TseI GCWGC 7 cut(s) 206, 1326, 1839, 2172, 2918, 3089, 3836
TspGWI ACGGA 9 cut(s) 146, 827, 869, 972, 997, 1445, 2573, 3504, 3768
TspRI CASTG 9 cut(s) 364, 434, 1144, 2130, 2569, 2801, 3210, 3465, 3725
Van91I CCANNNNNTGG 1 cut(s) 1655
VpaK11BI GGWCC 3 cut(s) 412, 1405, 2297
VspI ATTAAT 1 cut(s) 1098
XceI RCATGY 2 cut(s) 1146, 2861
XcmI CCANNNNNNNNNTGG 2 cut(s) 2032, 2835
XhoI CTCGAG 2 cut(s) 1364, 3850
XmiI GTMKAC 2 cut(s) 627, 2800
XmnI GAANNNNTTC 3 cut(s) 510, 890, 2202
XspI CTAG 5 cut(s) 33, 725, 792, 1760, 3345
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.