Rw1G013770

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016427
Physical Location & Seq
Forward (+)
30725264 .. 30737700
12437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G013770.1

Sequence Viewer

Length: 9315 bp
ATGCATGATGTTTCTACCTTAACTTTTTTTTTTTTTCCCAGGTTGGAAACCTCTGTTATTGAAGAGATAGTAAAATGTGTCGAGACAAAATGGAAGCTCAAATTTAATCCTGCATTCTCAATTGAGGATTCCAAGGATTTTGTTGGGATGGATCGTGTGAGGAAAATCGAATTGGACTTGGATATAGAGACATTGGATAAAATCGGTGTCATTGGAATTTGCGGGATGCCAGGAGTCGGTAAGACGACGATTGCAGAAGTTGTGTTTCGAAGAAATTGGCACCATTTTGAAGCCAACAGTTTCCTAGCTAATGTTGGAGAGATAAGTAAGACGAAAGGATTATTGCATATACAGAAGCAACTTTATCGCGACTTGTTGGATGGCAAAGTTATAACTATGAATGATGTCCCTGTGGAAAGGAGAAAATTGCGTCACAAAAAGGTGCTTATCATTCTTGATGGTGTGACCGAGTTACAATCTGTTTTTGAGCTAGCAGGAACAGATGTTAAAGAATTAATCAATTGGTTTGGTCGAGGCAGTAGAATCATTATAACCACAATAAATGAAAAGTTGTTGAAGGATTATGGGGCAAAAACACACAAGGTTGAGACACTTTCTGAAAATGAGCCTCGTGTGCTCTTCTTCCGGAAAGCCTTCAGCAAAGACCATCCTCTGCATGATTGTACTGAGTTGGAGTTCAAGTTCAAGATAGACCATACTATGCGTCAGTATGCTGAGTTGGCTTACAAATTTGTGGATTTAACTGATGGTCTTCCTTTGGCTATTGTAGTTTTGGGAAGCTTCACACTAGAAGCTAATAATGATTCTAATGATGAGAAAACTTTTGGGATTCTTAAGCTATGTATTGATGGATTACGTGATGGAGGAAAGGAAATTTTCTTGGACATTGCATGCTTCTTTAAAGGAGATGACAAAAATCGTGTGGAAAGGATATTTGACAGCTGCGGACTCAATCCACGCATTGAATTGAACATTCTCATCGAGAAATCTCTAGTGACAATTGTGGGAGGAAAATTGTGGATGCATGATTTACTACAAAAAGCTGGCCAGAAAATTGTTATTCGAAAATCCGAATTACCAGAGAATCGCAGCAGATTGTGGGATCATGAAGAAGCCGTTTCGGTACTTAAACAGAATAAGGGAACAAGCGCTGTCAAAGGTATTTTCTTAAGCTCACCTCAACTCTGCAAAGTGCGATTGAAGGCCGACTCATTTTCAAAAATGGACTTTCTTAGATTGTTGAAAATAAAGAATGTGAAATTTTCTGGATGCCATGAATACGATCTTTCAAATAAATTAAGCTTCTTGGAATGGCATGACTTTCCTTCAACATTTCTGCCATCTGAGTTTGAACCGAAGAATCTTGTTGAGCTGCACTTGCCTCGAAGCCAAGTAAAGCAATTATGGGAGGATAATATAACGCCTCCTTTGGAAAAGTTGGTTGTAATGGACCTTAGTGATTGCAAAGAGTTGACCACTACCCCAGACTTCCAGTCAGTGCCAAATCTTGAGAGCCTAGTCCTCAAAGGTTGTACAAGATTGTCTGAGGTAGACCTACCCAATGGAGATCAGCTCCAAAAGCTCATTTCATTGAATCTGGAAGGTTGCGAGTCTCTTAGTAGTCTTCCCAACGGTATCTGCAGCTTGAGATCTCTCACAACTTTCATTCTTTCGGGATGTTCCAGACTTGCAAATCTTCCGGAGAAGATTGGGGAAAACATGAAACAATTATCCGGACTTGATTTGGAGGGGACAGCTATAAAAGGGCTACCAACATCAATTAAGCACTTGACTGGTCTTATTCGGCTCAATCTAAAAGACTGCAAGAACCTTTTAAGTCTGCCAGCTGTCATTTGTAGTTTGACAAAACTTCAAGTTCTCAACCTGTCTGGCTGCTCAAATCTTGCTGAGTTGCCAGAAAACTTGGGAAGTTTAGTAGGTTTGAAGGAACTTGATGCAAGCAGAAGTTATATAAGACGAGTACCTTCCTCCATTCTACTTCTGAAGGACCTTGAAAGTTTATCTTTTTGTGGATGTGGAGGTATGCCGCCATCGGCATGTCCCATGGGTTTGCAGTTGCCTGCTTCATTCTCGGGATTAGATTCTTTGACAAAATTGGATCTAAGCTATTGCAAACTGTCTGGTGGAGCAATCCCTGCTGATCTTGACTGCTTATCTTCACTTAATCATCTAAATTTAAGTGGGAACAATTTACTGAGTGTACCGGAAAGCATCACTAAACTTTCTAACCTTGAAGTCCTTCTATTGAATGATTGTAGCGAGCTTCGATCATTACCACATCTTCCACCAAGTATACGACGTGTTTTTGCACGGAATTGTCCTCTGCTCAGAACTTATTCAAATAAATTAACTGTGTGGAGTTCAACTGCTACAGGATTTTGTTTCATAAATTCCCAAAGTAGTGAAGAACGTAAACTTCAGAGGATTCCGATATCAGAACAACGTCTTCTGCGGCCTCATTTGCAAAAGTTCTATGAGGATATAATTTATAAAGGTTGTGGTTTTGAATATATTACTCCTCACACTGAAATTCCATCATGGTTCGATCGCCGGGGTACAGGGTCGTCTATAACACTTCAGCTACCTCTAGATTATTTCGATGGCAAACGTGAGTTGATGGGACTTGCTCTATGTTTTGTTTATGAAGCGCAGGAGCATGAGAATATGGAATCAACAGCTTCTTACATCTCGGCTCAGGAAGTAAAATATAGTTGCAATTACAGTTGTCGTTTGTCCACATATGGAGGTCCTCATGATGACTGTCAGCTCCTCGTGTGTAAACTCATGGATCATGATTATGTTGGATCTTTTCGACATTGGGTCTATATAGCAGGAGGGCTGTTTGCAGAAAGGTTGATCAAACGCTCTGTTCGAGCTGAAGTTAAAATCAACAGACCAGGGGTGAAAGTGAAAGCCTGTGGGGCAAGTCTAATATACTCTGGAGGTGTGACAGAATTTGTTTGGAATTTGAATCTCCGTGATGAAATTCATATGAGAAATCTGTCGAGTGGGGATCAAAAGGAAAATTCTGACGATCAGTTTAATAAACAAGAACAAGAAACTATTGGTGATCATCAGCTGGAACTAAAACATGTACCTGTCCAATATCAGGCCCAAATGAACCACATGATCTCATTGTATGATACCAAGCGATCCCTTGATCATGATGCCGGATCATCTAGTAGAACCAGCAGCAGCACTGAAGAACCTCATCAAAGATTAGAAATACCAAAATCCAATGATCCGTCCAACAAACAAGATCAAGAGACTAATTATGGTTGTGCTACAGACAAAAAATTCTTGATTGAAATTGAACCACACAATTCAAGAGATAGTTCAACTTCAACCACGATCTTTGATGATCAATTGAGAACAAATGTAGAGTCATTCCTTGAGGAACTATTTGAGCTAGGTGGCAAAGCACGTCGCAATGATTCAGGTTTCATTTTCCCTCTCAGAGCAATTTTGCCGTGGTTTAATCATCAATGCATTGGGAACGTATTCATTCCAGAAAAAGAAAGAATTGGGAGTTCAGTAAGCCTACCTCTTCCTCTAGAATTGTATAACAATAAGGAATGGGTAGGATTTGCTGTATATGTGGCCTTCACTCTGCCTCCAGGTGTTTCACTTGAAAAATCATGTTGCTTCAAATCTGTATTGTATACTAGTGATGGAAATTTTTATTGCCTAAAATTATCGGCGGCTTGTGAAGACAATTTTAGGGGAGCACATCGACTCCTTGTCATCCACATACCACGAGGGCATTTTAAAAAACAGTTGAATGAGTGCGTGGCAATTCGTGCTGCTTTTGTCTCCTCAACTCCAAGTATGGAGGCTGAGATGTGTGGGATCCGTGTAGTATACGAGCAAGATTTGAAAGGGTTAATCCAAACAATTACCCAATGCACCACAAGAGATCCGCCTGCAGTTTACTATGGACTAGCTCATTCATTCGTGCCAGATGGAAGAGATTCTTTCATGTTAGCTATTATGGAGGATAATTTGATTGAAGCAGCTAAATCCAGCAAAAGGAAGCTTTCAACCTTGCAAGAGCCTTTAACAGATTCAAAGTTCCTCAGTGGTCAACAAATTTACTTAGAGATAGAGGCTGGAACTCCATACATGCCACACAAGTTCAAAAATTCCATAGTTGACTGGAAAAAAAACCTTGAGCTTTTGCTTGAATGTTTCTTTACCCTTTCTGATCTATCTATTAATCTCACTCTTCACAGAAGCACAACCTCTTTCTCAAGGGATTTTGATCCTTGTTCTGGTATTTTCCTTCAAAGAGAAATTCCAGAGTGGTTCACACCACAATATCCCTCCGAGAGTATGGTGTTGGAACTTCCTCCAAATTTGGAAAATGATTTGAATTTCAATGGACTTGTTGTATGTGCTGCCTTTTCTGTCCATGCCTCTGAAGCTGACGTCCTCAAATATCTGAAATCAGGACTCCATCCCCAACTTACGTGCCATTTCCAAAATTTGAGTTCTGAATCTTCTGATCAATCTTATGGTCTTACAGCTCCCATGATTGATACCAGTCCCGAGAACAAATTCATGTGGTTATATCGCAGAGGATTCATTTGGCAGGAATACCTACCCCGTGACTTATTTAAGGATCTGCTGCATAAAGATTCTTCAGTCAAGGTTTCATTTAGGAGTGATTCTTCCCAAGTCTTGGAGGGTGAGACTAGTGGAAAAGCCAATGATCAGCTGGAACTAGGACATGTACCTGTCCAATATCAGCCCCAAATGGACCGCATGATCTCTTTGTATGATACTAAAGCAAGTAACATGCAATCCCTTGACCATGATGCAGGATCATCTAGTAGAACTGGCAGCAGTACTGAAGAACCTCTTCAAATATTAGAAAGACCAGAATCCAATGATCGGTCCGATGAACAAGTGCACGAAACTAATTCTGACCTTACACATGTAGCAGAATGTGGTTATGCTTCAGACACTAAATTCTTGATTGAAATTGAACCACACACTTCAAGAGATAATTCAAGTTCAACCAAGATTGTTGGTGATCAATTGAGAACAAATCTGGGAGGCAAAGCACGTCGCTATGATTTAGGTTTCATTTTCTCTCTTCGAGCAATTTTACCGTGGTTTAAACATCAAGGCATTGGGGACGTGATAGGTGTACCTCTTCCTCTAGAATTGTATAACAACAAGGAATGCATAGGATTTGCTCTATATGTGATCTTCACTCTGCCCCCAGGTGCTTGGCTTAAAGAAGGTTCTTGTTTTAAAATTGAGTTTACTGAAGAGCCAAGGTATTGCCTAAACTTAGCGTCCGCTGGTGAAGACAATTTCAGGGGTTCACATCGACTCCTGGTTATTCACATACCACAAAGACATTTTGGAGAACAGTTGAACCAGTGGAGTACCATTCGTGCTTCTTTTGAATCCCTTTTGCCAGGTGTGGAGGTTGAGATGTGTGGCATGCGTGTAGTATACCAGGAAAATTTGAAAGGGTTAATCCAAACAATTACCAACTGCACAATATGCAGTCCCCCTGCTTACTATGGACTTGCCCAACCATTTGTGCCGGGAAGGTGGTCTTATGTGTTCACACACATGAGGAATAGTTTGGTTGAAGCAGCTAAATCCAGCAAGAGGAAGCTTTCAACCTTGCAAGAGCCTTTAACAGATTTAAAATCCATCCAAGGAATTCAGTTACTGATACTTGCTGCAACTCCATTTACTTCACTCAAGAAAACAGAAACAGTAATCAAGTGGAAAAGAGACCTTAAGCTTTTGCTTCAACGTTTCTTAACCCTTTCTGGTCTATCAATAAGTCTCATTCTTAGAGGAGGCAGAATTTCTTTCTCAGAGGATCATAATCTTCATAGTGGTATTTTCTTTCAAAAGGAAATTCCAGACTGGTTTACACCACATCACTCTGCCAGTATGGTGCCCGAACTTCCTCCAGATTTGGAAAATGATATGAATTTGATGGGTTTTGTTGTATGTGCTGCCTTTTCAGTCCACGCCTCTAAAGCTGATGTCCTCAAATATCTGGATTCGGGACACCATCCCGAACTTATGTGCCATTTCCAATCAAGTTCTGACTATTCTGGTGTAGTAGTTCGTAAGATGGTTGGCATCAGTCCGAAGAACAAATTCATGTGGTTTTATCGTAGTGGATTCATTTGGCTGCATTACGTGCCCCGTCACAATGTTGAGCATCTCTTCAATACACTATCTGAACTAAAGGTTTCATTTGAGAGTGATTCTTCCCAAGTATTGGCAGTACAGAAATGTAGCTTCCGTTTCTTATACAAGCAAGATGTAGAAGGATTTAAGCAAACAATAATAGAGTGCTTTACCTCCTTCTTTGACAACGAGGATATCATTAAGCCATATATAGAAGAAGAGGACTCTGGACATAATAAGTGGCCAAATCATGGAGATGAGATGGGACCATCTAGTACAACTGGTGGCAACATTGTCATTGAGAAAACTCACAATGAGAGATTAGATGTACCCATAACTGATGGGAAGGGGAAAGGCCCTTCTGATGTCTCCTCAGTTGAAGCAGATGAGAGAGATTATCATGGAACTACACCTGTTTGTCAGGGTGAAACTAGTGGACAAGCCAGTCATCAACTGGAACCAGTTACACATGTAGTAGAAGAAGAACATGGTTCTAGTATTTCTCCTCAACAGCTAATCCCATTCTTATCTGAAATTGAACCTCCCGCCTCAACTGCAGGTTCAAGTTCAACCAAATTTGAAGGTGATCAGTTCAGAACTAGTGGAAAATGCAGTGATCAGCTGGAACTAGTTACACATGTAGTAGAAGAAGAACACGGTTCTAGTTGTCCTCCGGAGCTAATCCCATTCTTATTTGAAATTGAACCTTCCGCCTCAACAGATTCAAGTTCAACCAAATTTGAAGGTGATCAGTTCAGAACAAAAGTGGAGGCTTTGCTCACAGTAATGTTTCGACATAAGGATAAGAAATGGGCAGGGTTAGCTCTATATGTTGCCTTCACTGTGCCCCCAGGTTATACGGAAAACATTCTATGTCGCTGTTCTTTGTCTACCTCTGCAGGACATGCAGTAGCGATCACAATAGCATGCTCCCAAGGCAATTTTTCAGGATCACGACGACTCTGTTTCATTCATAAACCGCGTGCACGTTTTCCAGAACAGTGGAATACGTGTAGCTATATTTCTGCCTTCTTTGAAGTCCTTACTCCAGTTGTGGATGTTGAGATGTGTGGGGTCCATCTACTATACGAGCAAGATCTGATAGGGATAATCCAAACAATTACCCACTGCACTATCAGGAGTCCGCCTGTTTACTATGGGATTGGATTTGCTAACACACCACGTCAGTCGCAAATGGGTCAGCCTTTTGCGGATATAAATATGGACACAAATTTGAATGAACTCGCTGCAAGGACTCCATCTACCGCTCAAGAGCCTGTAAAGACCCTTGTTGGTCAAGTAATTCATTTACGCATACATGGTGACACCCCATTCAAGAAAAAAGATTCAGTGATGGAGTGGAAAAAAAACCTTCAGTTTTTGCTTCAATGTTTCTTTAGGTTTCCTCTATCTATAACCCTCACTCTTGGAGGAGACGTCATCTCTAGCCTAGGTGATTCTGATCCTTACTGTCTCTGCAGCTCAAGTATTATCCCTCAAAAGGACATTCCGAAGTGGTTCTGTGGGGTGAAATATCCCACAAATAAGTTGAAGACCACACTCCCTCCGAATTTGGAAAATGATGAGAACTGGTTGGGATTTGTTGTCTGTGCTGCGTTTTCAGTCCATGCGTCCCAAGATGACGTCCTCAGTTATCTGGATTCAGAAAACTATCCGAAACTTACATGCCTTTTGCAGTGGGGTTCTGAAAGAGCTTTAAATCTCAAAGCTGGCAAGAAGTCGAGCATTGTTCTCAAGAATGAATTCATGTGGTTATATCTTAGACGATTCATTTGGCTGCGATACATACCCCATCAGAAATTTAAGGATCGGTTACATATATCTGATCACCTAGAGGCTTCATTTAGGACTGATTCAATTTCTTCCAAAGTCTTGAGGATACAGGAATGCAGCCTGCGTGTTCTATACAAAAAAGACGTGGAAGAATTTAAGCAAACAATAATAGAATGCTTTACCTGCTTCTTTGACAACGAGGATGTCATTCGGCCGTATATAGGAGAGGATTCCAGACATAACAAGTGGCCAAGTCATGCAGATGACAGAGATTCTTATGGCACTACACCTGTTTGTCAGGGTGAACGAGGTTCTGCATCCAGTACTTCTCCGGAGCTAATGCCATTTTTCATTGAAACTGAACCTCCAGCTTCAACGGATTCAAGTTCAACCAAAGTTACACGGCAACTGAGAATGATAGAGTCATCGCTTGAACAACTATTTCAGGTTCGGAGGTTGAAATGTGTGGGATCCGGGCTTGATCTGTCCGTAAAACTCGTTCTCGGAGAAGACACCATCGCTTTCTATCAGGATTGCTTCAACCATGCTTGTCTTTTCCCTCAAAAGAAGATTCCAGATTGGTTCAAAGAAAAATCTCTCACATCTACGTATGTGTACCCACTTCCCGATGATTTGGAAAGTGACAACAATTGGATGGGACTTGTTATATGTGCTGCCTTTTCAGTACACCCACTTCCAACTGACCTTGGCAATTATAAGGACTCAGAACTGAGTTCTGATGTTAAGTGCAATTTTCGGTGGAGTTCTAATCCTTATCGATTCATTTCTATCAATGAGGGTGTGTTCGGCGTCAGGCCAAACAACAAATTCATGTGGTTATCTCGTAGAGGATTCATTTGGCTGCAATTCATATCTGGTGAAATTTTTAAGTTTGCCATGGATAAACATCGTCGTCAACTAGAAGCTTCATTTGAGAGTGGTTCCCCAGCTTTGTCGGTACAGGAATGTAGCTACCGAATCTTATACAAGCAAGACTTGCAAGAATATAAGCAAACAATTATACAGTGCTTCGCCTCTGTCTTTGACAACTTGGATCACAGTCTACCATATAAGGAAGACGATTCTCGACATAACGAGGAGCCAATTCATGAAGATGATCAGATGGACTCTTTAGGAACTACAAGCTTTTCTGAGGACACCCCCCATGGAAGAGAAACAACAGTGGTTCTTCCTGAAAGTGAAACCAGTGGAAATTCCTTTAATCGTTTCAACAGCATACTGTTTGAGCCCATTTTGTACGACAACAAAACGGCAGTAATTTTCGCAGATTTTCGAGGATGGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

3104

Amino Acids

350.94

Weight (kDa)

5.83

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 66 - 222 2.3e-12 NB-ARC domain
WHD_ROQ1 PF23282 297 - 362 4.4e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 527 - 637 2.8e-10 Leucine-rich repeat region
LRR_13 PF23286 556 - 644 4.9e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 625 - 690 2.1e-06 Leucine-rich repeat region
LRR_13 PF23286 643 - 799 2.9e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 854 - 993 1.6e-08 C-JID domain
C-JID PF20160 1974 - 2117 1.5e-06 C-JID domain
C-JID PF20160 2569 - 2718 1.5e-06 C-JID domain
C-JID PF20160 2854 - 3001 2.6e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 392, 551, 2532, 8721
AasI GACNNNNNNGTC 1 cut(s) 6654
AatII GACGTC 3 cut(s) 4473, 7650, 7857
Acc36I ACCTGC 2 cut(s) 6758, 8195
AccB1I GGYRCC 2 cut(s) 279, 5968
AccB7I CCANNNNNTGG 3 cut(s) 4723, 6301, 6461
AccBSI CCGCTC 1 cut(s) 7478
AccI GTMKAC 7 cut(s) 1572, 2335, 3704, 3903, 5508, 7100, 9067
AccII CGCG 2 cut(s) 369, 7192
AccIII TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
AclI AACGTT 1 cut(s) 5821
AcoI YGGCCR 4 cut(s) 1066, 6452, 8216, 8252
AcyI GRCGYC 4 cut(s) 4470, 7647, 7854, 8814
AfeI AGCGCT 1 cut(s) 1171
AflII CTTAAG 3 cut(s) 854, 1189, 5804
AflIII ACRYGT 7 cut(s) 2341, 3133, 4771, 4978, 6679, 6847, 7220
AhdI GACNNNNNGTC 3 cut(s) 1513, 2861, 3782
AhlI ACTAGT 5 cut(s) 3707, 4736, 6641, 6809, 6838
AjiI CACGTC 6 cut(s) 2342, 3467, 5111, 5185, 7394, 8149
AjnI CCWGG 9 cut(s) 38, 229, 2938, 3658, 5269, 5385, 5470, 5511, 7060
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AloI GAACNNNNNNTCC 2 cut(s) 686, 718
Alw21I GWGCWC 4 cut(s) 639, 3772, 4956, 7198
Alw44I GTGCAC 2 cut(s) 4952, 7194
AlwNI CAGNNNCTG 1 cut(s) 5734
Ama87I CYCGRG 2 cut(s) 2113, 4589
Aor13HI TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
Aor51HI AGCGCT 1 cut(s) 1171
ApaLI GTGCAC 2 cut(s) 4952, 7194
ArsI GACNNNNNNTTYG 4 cut(s) 3410, 3442, 7986, 8018
AseI ATTAAT 2 cut(s) 515, 4257
AspA2I CCTAGG 1 cut(s) 7660
AspLEI GCGC 2 cut(s) 1172, 2692
AspS9I GGNCC 9 cut(s) 1471, 2029, 2789, 3154, 4801, 4938, 6476, 6566, 7285
AsuC2I CCSGG 3 cut(s) 2594, 5604, 8479
AsuII TTCGAA 2 cut(s) 268, 1084
AsuNHI GCTAGC 1 cut(s) 490
AvaI CYCGRG 2 cut(s) 2113, 4589
AvaII GGWCC 7 cut(s) 1471, 2029, 2789, 4801, 4938, 6476, 7285
AvrII CCTAGG 1 cut(s) 7660
BaeGI GKGCMC 5 cut(s) 4956, 5973, 6225, 7059, 7198
BalI TGGCCA 3 cut(s) 1068, 6454, 8254
BamHI GGATCC 2 cut(s) 3891, 8474
BanI GGYRCC 2 cut(s) 279, 5968
BanII GRGCYC 1 cut(s) 9255
BarI GAAGNNNNNNTAC 2 cut(s) 8682, 8714
BauI CACGAG 3 cut(s) 630, 2813, 3798
BbsI GAAGAC 8 cut(s) 764, 1637, 2480, 3759, 5364, 7769, 8520, 9087
Bbv12I GWGCWC 4 cut(s) 639, 3772, 4956, 7198
BceAI ACGGC 5 cut(s) 1121, 3496, 8203, 8423, 9291
BcgI CGANNNNNNTGC 4 cut(s) 347, 381, 1385, 1419
BciT130I CCWGG 9 cut(s) 40, 231, 2940, 3660, 5271, 5387, 5472, 5513, 7062
BciVI GTATCC 1 cut(s) 8103
BcnI CCSGG 3 cut(s) 2594, 5604, 8479
BcuI ACTAGT 5 cut(s) 3707, 4736, 6641, 6809, 6838
BfmI CTRYAG 7 cut(s) 1660, 2412, 3327, 3966, 6765, 7107, 7687
BfoI RGCGCY 1 cut(s) 1173
BfrI CTTAAG 3 cut(s) 854, 1189, 5804
BfuAI ACCTGC 2 cut(s) 6758, 8195
BfuI GTATCC 1 cut(s) 8103
BglI GCCNNNNNGGC 1 cut(s) 2963
BglII AGATCT 2 cut(s) 1670, 7306
BlnI CCTAGG 1 cut(s) 7660
BmcAI AGTACT 2 cut(s) 4891, 8329
Bme18I GGWCC 7 cut(s) 1471, 2029, 2789, 4801, 4938, 6476, 7285
BmeRI GACNNNNNGTC 3 cut(s) 1513, 2861, 3782
BmeT110I CYCGRG 2 cut(s) 2113, 4589
BmgBI CACGTC 6 cut(s) 2342, 3467, 5111, 5185, 7394, 8149
BmgT120I GGNCC 9 cut(s) 1471, 2029, 2789, 3154, 4801, 4938, 6476, 6566, 7285
BmiI GGNNCC 9 cut(s) 281, 3893, 5970, 6477, 6669, 7286, 8476, 8947, 9105
BmtI GCTAGC 1 cut(s) 494
BpiI GAAGAC 8 cut(s) 764, 1637, 2480, 3759, 5364, 7769, 8520, 9087
BplI GAGNNNNNCTC 2 cut(s) 1578, 1610
BpmI CTGGAG 5 cut(s) 3003, 3642, 5967, 7242, 8355
Bpu10I CCTNAGC 1 cut(s) 2736
Bpu14I TTCGAA 2 cut(s) 268, 1084
BpuMI CCSGG 3 cut(s) 2594, 5604, 8479
Bsa29I ATCGAT 1 cut(s) 8782
BsaAI YACGTR 5 cut(s) 878, 4512, 6220, 7221, 8613
BsaBI GATNNNNATC 4 cut(s) 4302, 5895, 7671, 8910
BsaHI GRCGYC 4 cut(s) 4470, 7647, 7854, 8814
BsaI GGTCTC 1 cut(s) 5793
BsaWI WCCGGW 6 cut(s) 645, 1720, 1754, 2245, 6883, 8335
Bse3DI GCAATG 2 cut(s) 906, 3478
Bse8I GATNNNNATC 4 cut(s) 4302, 5895, 7671, 8910
BseAI TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
BseBI CCWGG 9 cut(s) 40, 231, 2940, 3660, 5271, 5387, 5472, 5513, 7062
BseCI ATCGAT 1 cut(s) 8782
BseJI GATNNNNATC 4 cut(s) 4302, 5895, 7671, 8910
BseMI GCAATG 2 cut(s) 906, 3478
BseRI GAGGAG 8 cut(s) 2550, 2801, 3847, 5880, 6571, 6705, 7656, 9116
BseSI GKGCMC 5 cut(s) 4956, 5973, 6225, 7059, 7198
BseX3I CGGCCG 1 cut(s) 8216
BseYI CCCAGC 1 cut(s) 8950
BsgI GTGCAG 3 cut(s) 1379, 5536, 7324
Bsh1236I CGCG 2 cut(s) 369, 7192
Bsh1285I CGRYCG 2 cut(s) 2590, 8219
BshNI GGYRCC 2 cut(s) 279, 5968
BshVI ATCGAT 1 cut(s) 8782
BsiEI CGRYCG 2 cut(s) 2590, 8219
BsiHKAI GWGCWC 4 cut(s) 639, 3772, 4956, 7198
BsiHKCI CYCGRG 2 cut(s) 2113, 4589
BsmBI CGTCTC 1 cut(s) 7638
BsmI GAATGC 4 cut(s) 113, 5234, 8123, 8183
Bso31I GGTCTC 1 cut(s) 5793
BsoBI CYCGRG 2 cut(s) 2113, 4589
Bsp119I TTCGAA 2 cut(s) 268, 1084
Bsp1286I GDGCHC 8 cut(s) 639, 3772, 4956, 5973, 6225, 7059, 7198, 9255
Bsp13I TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
Bsp1407I TGTACA 1 cut(s) 1553
Bsp19I CCATGG 3 cut(s) 2085, 8901, 9169
Bsp68I TCGCGA 1 cut(s) 369
BspDI ATCGAT 1 cut(s) 8782
BspEI TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
BspFNI CGCG 2 cut(s) 369, 7192
BspHI TCATGA 5 cut(s) 1126, 2794, 2833, 3205, 9112
BspLI GGNNCC 9 cut(s) 281, 3893, 5970, 6477, 6669, 7286, 8476, 8947, 9105
BspMAI CTGCAG 5 cut(s) 1664, 3970, 6769, 7111, 7691
BspMI ACCTGC 2 cut(s) 6758, 8195
BspOI GCTAGC 1 cut(s) 494
BspQI GCTCTTC 2 cut(s) 644, 5313
BspT104I TTCGAA 2 cut(s) 268, 1084
BspT107I GGYRCC 2 cut(s) 279, 5968
BspTI CTTAAG 3 cut(s) 854, 1189, 5804
BspTNI GGTCTC 1 cut(s) 5793
BsrBI CCGCTC 1 cut(s) 7478
BsrDI GCAATG 2 cut(s) 906, 3478
BsrGI TGTACA 1 cut(s) 1553
BssNAI GTATAC 4 cut(s) 2336, 3705, 3904, 5509
BssNI GRCGYC 4 cut(s) 4470, 7647, 7854, 8814
BssSI CACGAG 3 cut(s) 630, 2813, 3798
BssT1I CCWWGG 9 cut(s) 132, 2085, 5324, 5719, 7144, 7660, 8710, 8901, 9169
Bst1107I GTATAC 4 cut(s) 2336, 3705, 3904, 5509
Bst2BI CACGAG 3 cut(s) 630, 2813, 3798
Bst2UI CCWGG 9 cut(s) 40, 231, 2940, 3660, 5271, 5387, 5472, 5513, 7062
BstACI GRCGYC 4 cut(s) 4470, 7647, 7854, 8814
BstAFI CTTAAG 3 cut(s) 854, 1189, 5804
BstAPI GCANNNNNTGC 6 cut(s) 2177, 3842, 5559, 6220, 7115, 9001
BstAUI TGTACA 1 cut(s) 1553
BstBAI YACGTR 5 cut(s) 878, 4512, 6220, 7221, 8613
BstBI TTCGAA 2 cut(s) 268, 1084
BstDSI CCRYGG 5 cut(s) 2085, 3512, 5156, 8901, 9169
BstFNI CGCG 2 cut(s) 369, 7192
BstH2I RGCGCY 1 cut(s) 1173
BstHHI GCGC 2 cut(s) 1172, 2692
BstMCI CGRYCG 2 cut(s) 2590, 8219
BstNI CCWGG 9 cut(s) 40, 231, 2940, 3660, 5271, 5387, 5472, 5513, 7062
BstSFI CTRYAG 7 cut(s) 1660, 2412, 3327, 3966, 6765, 7107, 7687
BstSLI GKGCMC 5 cut(s) 4956, 5973, 6225, 7059, 7198
BstSNI TACGTA 1 cut(s) 8613
BstUI CGCG 2 cut(s) 369, 7192
BstV2I GAAGAC 8 cut(s) 764, 1637, 2480, 3759, 5364, 7769, 8520, 9087
BstX2I RGATCY 8 cut(s) 1670, 2140, 2846, 3891, 3958, 4663, 7306, 8474
BstXI CCANNNNNNTGG 2 cut(s) 5277, 7212
BstYI RGATCY 8 cut(s) 1670, 2140, 2846, 3891, 3958, 4663, 7306, 8474
BstZ17I GTATAC 4 cut(s) 2336, 3705, 3904, 5509
BstZI CGGCCG 1 cut(s) 8216
Bsu15I ATCGAT 1 cut(s) 8782
BsuI GTATCC 1 cut(s) 8103
BsuTUI ATCGAT 1 cut(s) 8782
BtgI CCRYGG 5 cut(s) 2085, 3512, 5156, 8901, 9169
BtgZI GCGATG 2 cut(s) 8415, 8506
BtrI CACGTC 6 cut(s) 2342, 3467, 5111, 5185, 7394, 8149
BtsI GCAGTG 3 cut(s) 6829, 7336, 7913
BtuMI TCGCGA 1 cut(s) 369
BveI ACCTGC 2 cut(s) 6758, 8195
CaiI CAGNNNCTG 1 cut(s) 5734
CciI TCATGA 5 cut(s) 1126, 2794, 2833, 3205, 9112
CfoI GCGC 2 cut(s) 1172, 2692
Cfr13I GGNCC 9 cut(s) 1471, 2029, 2789, 3154, 4801, 4938, 6476, 6566, 7285
ClaI ATCGAT 1 cut(s) 8782
CpoI CGGWCCG 1 cut(s) 4938
CseI GACGC 5 cut(s) 419, 713, 5334, 7830, 8803
CspCI CAANNNNNGTGG 2 cut(s) 3379, 3414
CspI CGGWCCG 1 cut(s) 4938
DraI TTTAAA 6 cut(s) 922, 3811, 5164, 5302, 5709, 7929
DrdI GACNNNNNNGTC 1 cut(s) 6654
DriI GACNNNNNGTC 3 cut(s) 1513, 2861, 3782
DseDI GACNNNNNNGTC 1 cut(s) 6654
EaeI YGGCCR 4 cut(s) 1066, 6452, 8216, 8252
EagI CGGCCG 1 cut(s) 8216
Eam1105I GACNNNNNGTC 3 cut(s) 1513, 2861, 3782
EciI GGCGGA 3 cut(s) 3951, 6910, 7344
EclXI CGGCCG 1 cut(s) 8216
Eco105I TACGTA 1 cut(s) 8613
Eco130I CCWWGG 9 cut(s) 132, 2085, 5324, 5719, 7144, 7660, 8710, 8901, 9169
Eco24I GRGCYC 1 cut(s) 9255
Eco31I GGTCTC 1 cut(s) 5793
Eco32I GATATC 2 cut(s) 2475, 6406
Eco47I GGWCC 7 cut(s) 1471, 2029, 2789, 4801, 4938, 6476, 7285
Eco47III AGCGCT 1 cut(s) 1171
Eco52I CGGCCG 1 cut(s) 8216
Eco88I CYCGRG 2 cut(s) 2113, 4589
EcoO109I RGGNCCY 3 cut(s) 2029, 2789, 6566
EcoRI GAATTC 2 cut(s) 5724, 7973
EcoRII CCWGG 9 cut(s) 38, 229, 2938, 3658, 5269, 5385, 5470, 5511, 7060
EcoRV GATATC 2 cut(s) 2475, 6406
EcoT14I CCWWGG 9 cut(s) 132, 2085, 5324, 5719, 7144, 7660, 8710, 8901, 9169
EcoT22I ATGCAT 4 cut(s) 6, 1047, 3533, 5234
EcoT38I GRGCYC 1 cut(s) 9255
ErhI CCWWGG 9 cut(s) 132, 2085, 5324, 5719, 7144, 7660, 8710, 8901, 9169
Esp3I CGTCTC 1 cut(s) 7638
FauI CCCGC 2 cut(s) 215, 6763
FauNDI CATATG 2 cut(s) 2782, 3033
FblI GTMKAC 7 cut(s) 1572, 2335, 3704, 3903, 5508, 7100, 9067
FriOI GRGCYC 1 cut(s) 9255
GlaI GCGC 2 cut(s) 1171, 2691
GsaI CCCAGC 1 cut(s) 8954
GsuI CTGGAG 5 cut(s) 3003, 3642, 5967, 7242, 8355
HaeII RGCGCY 1 cut(s) 1173
HgaI GACGC 5 cut(s) 419, 713, 5334, 7830, 8803
HhaI GCGC 2 cut(s) 1172, 2692
Hin1I GRCGYC 4 cut(s) 4470, 7647, 7854, 8814
Hin6I GCGC 2 cut(s) 1170, 2690
HinP1I GCGC 2 cut(s) 1170, 2690
HincII GTYRAC 4 cut(s) 1494, 4127, 4195, 8921
HindII GTYRAC 4 cut(s) 1494, 4127, 4195, 8921
HindIII AAGCTT 7 cut(s) 799, 1321, 4076, 5675, 5807, 8928, 9148
Hpy99I CGWCG 6 cut(s) 250, 2343, 3471, 5115, 7170, 8919
Hsp92I GRCGYC 4 cut(s) 4470, 7647, 7854, 8814
HspAI GCGC 2 cut(s) 1170, 2690
Kpn2I TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
LguI GCTCTTC 2 cut(s) 644, 5313
MbiI CCGCTC 1 cut(s) 7478
MfeI CAATTG 6 cut(s) 120, 520, 1020, 3407, 5081, 8653
MflI RGATCY 8 cut(s) 1670, 2140, 2846, 3891, 3958, 4663, 7306, 8474
MhlI GDGCHC 8 cut(s) 639, 3772, 4956, 5973, 6225, 7059, 7198, 9255
MlsI TGGCCA 3 cut(s) 1068, 6454, 8254
MluNI TGGCCA 3 cut(s) 1068, 6454, 8254
MmeI TCCRAC 8 cut(s) 24, 295, 357, 672, 2824, 3315, 4362, 8726
Mox20I TGGCCA 3 cut(s) 1068, 6454, 8254
Mph1103I ATGCAT 4 cut(s) 6, 1047, 3533, 5234
MroI TCCGGA 5 cut(s) 645, 1720, 1754, 6883, 8335
MscI TGGCCA 3 cut(s) 1068, 6454, 8254
MslI CAYNNNNRTG 7 cut(s) 2077, 2838, 5630, 6465, 6995, 8265, 9117
Msp20I TGGCCA 3 cut(s) 1068, 6454, 8254
MspA1I CMGCKG 6 cut(s) 963, 1868, 3121, 4759, 5351, 6832
MspCI CTTAAG 3 cut(s) 854, 1189, 5804
MssI GTTTAAAC 1 cut(s) 5164
MunI CAATTG 6 cut(s) 120, 520, 1020, 3407, 5081, 8653
Mva1269I GAATGC 4 cut(s) 113, 5234, 8123, 8183
MvaI CCWGG 9 cut(s) 40, 231, 2940, 3660, 5271, 5387, 5472, 5513, 7062
MvnI CGCG 2 cut(s) 369, 7192
NciI CCSGG 3 cut(s) 2594, 5604, 8479
NcoI CCATGG 3 cut(s) 2085, 8901, 9169
NdeI CATATG 2 cut(s) 2782, 3033
NheI GCTAGC 1 cut(s) 490
NlaIV GGNNCC 9 cut(s) 281, 3893, 5970, 6477, 6669, 7286, 8476, 8947, 9105
NmeAIII GCCGAG 1 cut(s) 2711
NmuCI GTSAC 8 cut(s) 431, 463, 1015, 2989, 4649, 6227, 7532, 8645
NruI TCGCGA 1 cut(s) 369
NsiI ATGCAT 4 cut(s) 6, 1047, 3533, 5234
NspV TTCGAA 2 cut(s) 268, 1084
PaeI GCATGC 3 cut(s) 915, 5500, 7140
PagI TCATGA 5 cut(s) 1126, 2794, 2833, 3205, 9112
PciI ACATGT 5 cut(s) 3133, 4771, 4978, 6679, 6847
PciSI GCTCTTC 2 cut(s) 644, 5313
PcsI WCGNNNNNNNCGW 1 cut(s) 2911
PctI GAATGC 4 cut(s) 113, 5234, 8123, 8183
PflMI CCANNNNNTGG 3 cut(s) 4723, 6301, 6461
Ple19I CGATCG 1 cut(s) 2590
PmeI GTTTAAAC 1 cut(s) 5164
Ppu21I YACGTR 5 cut(s) 878, 4512, 6220, 7221, 8613
PpuMI RGGWCCY 2 cut(s) 2029, 2789
PscI ACATGT 5 cut(s) 3133, 4771, 4978, 6679, 6847
PshBI ATTAAT 2 cut(s) 515, 4257
PsiI TTATAA 4 cut(s) 392, 551, 2532, 8721
Psp1406I AACGTT 1 cut(s) 5821
Psp5II RGGWCCY 2 cut(s) 2029, 2789
Psp6I CCWGG 9 cut(s) 38, 229, 2938, 3658, 5269, 5385, 5470, 5511, 7060
PspFI CCCAGC 1 cut(s) 8950
PspGI CCWGG 9 cut(s) 38, 229, 2938, 3658, 5269, 5385, 5470, 5511, 7060
PspN4I GGNNCC 9 cut(s) 281, 3893, 5970, 6477, 6669, 7286, 8476, 8947, 9105
PspPI GGNCC 9 cut(s) 1471, 2029, 2789, 3154, 4801, 4938, 6476, 6566, 7285
PspPPI RGGWCCY 2 cut(s) 2029, 2789
PsrI GAACNNNNNNTAC 2 cut(s) 8487, 8519
PstI CTGCAG 5 cut(s) 1664, 3970, 6769, 7111, 7691
PstNI CAGNNNCTG 1 cut(s) 5734
PsuI RGATCY 8 cut(s) 1670, 2140, 2846, 3891, 3958, 4663, 7306, 8474
PvuI CGATCG 1 cut(s) 2590
PvuII CAGCTG 5 cut(s) 963, 1868, 3121, 4759, 6832
RruI TCGCGA 1 cut(s) 369
RseI CAYNNNNRTG 7 cut(s) 2077, 2838, 5630, 6465, 6995, 8265, 9117
Rsr2I CGGWCCG 1 cut(s) 4938
RsrII CGGWCCG 1 cut(s) 4938
SapI GCTCTTC 2 cut(s) 644, 5313
Sau96I GGNCC 9 cut(s) 1471, 2029, 2789, 3154, 4801, 4938, 6476, 6566, 7285
ScaI AGTACT 2 cut(s) 4891, 8329
SduI GDGCHC 8 cut(s) 639, 3772, 4956, 5973, 6225, 7059, 7198, 9255
SfcI CTRYAG 7 cut(s) 1660, 2412, 3327, 3966, 6765, 7107, 7687
SfuI TTCGAA 2 cut(s) 268, 1084
SinI GGWCC 7 cut(s) 1471, 2029, 2789, 4801, 4938, 6476, 7285
SmiMI CAYNNNNRTG 7 cut(s) 2077, 2838, 5630, 6465, 6995, 8265, 9117
SnaBI TACGTA 1 cut(s) 8613
SpeI ACTAGT 5 cut(s) 3707, 4736, 6641, 6809, 6838
SphI GCATGC 3 cut(s) 915, 5500, 7140
SspI AATATT 1 cut(s) 4911
StyI CCWWGG 9 cut(s) 132, 2085, 5324, 5719, 7144, 7660, 8710, 8901, 9169
TaqII GACCGA 2 cut(s) 482, 4926
TatI WGTACW 7 cut(s) 683, 1553, 4889, 6307, 6485, 8327, 8689
TauI GCSGC 3 cut(s) 2071, 2497, 3746
TseFI GTSAC 8 cut(s) 431, 463, 1015, 2989, 4649, 6227, 7532, 8645
Tsp45I GTSAC 8 cut(s) 431, 463, 1015, 2989, 4649, 6227, 7532, 8645
TspGWI ACGGA 8 cut(s) 2368, 3008, 3276, 3884, 6314, 7085, 8396, 8482
Van91I CCANNNNNTGG 3 cut(s) 4723, 6301, 6461
Vha464I CTTAAG 3 cut(s) 854, 1189, 5804
VneI GTGCAC 2 cut(s) 4952, 7194
VpaK11BI GGWCC 7 cut(s) 1471, 2029, 2789, 4801, 4938, 6476, 7285
VspI ATTAAT 2 cut(s) 515, 4257
XbaI TCTAGA 3 cut(s) 2629, 3595, 5206
XcmI CCANNNNNNNNNTGG 2 cut(s) 4756, 6661
XmaJI CCTAGG 1 cut(s) 7660
XmiI GTMKAC 7 cut(s) 1572, 2335, 3704, 3903, 5508, 7100, 9067
ZraI GACGTC 3 cut(s) 4471, 7648, 7855
ZrmI AGTACT 2 cut(s) 4891, 8329
Zsp2I ATGCAT 4 cut(s) 6, 1047, 3533, 5234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.