Rh1DG166600

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0016427
Physical Location & Seq
Forward (+)
34215934 .. 34239477
23544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG166600.1

Sequence Viewer

Length: 8088 bp
ATGGCCGAGACGATGTCGTCGCCGTCGATCTGTGGGAGAAGCAAGTCGGCGGCGACAATGATCTGTGGGAGACAAGAAGTATTAAACACAACAAGACACAAACATAGTATTTCAGCTACCCAACAAGTAATAAACACAAGAAGACAAAAATTCGATAAAGAAACCCTAAATCCCACCAACCCAGATTCAATACATGCATATATAAACCAAAGTGCATTTCTTTCCCTGTTTAAAACCTCATATTTGATTGATATAATTTATAAAGGTTGTGGTTTTGAATATATTACTCCTCACACTGAAATTCCATCATGGTTCGGTCACCCGGGTAGAGAGTCGTCTGTAACACTCCAGCTACCTCTAGATTATTTCGATGGCAAACGTGAGTTGATGGGACTTGCTCTATGTTTTGTTTATGAAGCGCAGGAGCATGAAAATATGGAATCAACAGCTTCTAGCATCTCGGCTCAGGAAGTAAAATATAGTTGCAATTACAGTTGTCGCTTGTCCACATATGGAGGTCCTCATGATGACTGTCCCCTCCTCGTGTGTAAACTCATGGATCATGATTATGTTGGATCTTTTCGACATTGGGTCTATATAGCAGGAGGACTGTTTGCAGAAAGGTTAATCAAACGCTCTGTTCGAGCTGAAGTTATAATCAACAGACCAGGGGTGAAAGTGAAAGCCTGTCGGGCAAGTCTAATATACTCTGGAGGTGTGACAGAATTTGTTTGGAATTTGAATCTCCGTGATGAAATTCATGTGAGAAATCTGTCGAGTGGGGATCAAAAGGAAAAATCTGACGATCAGTTTAATAAACAAGAACAAGAAACTGTTTGTGATCATCTGCTGGAACTAAAACATGTACCTGTCCAATATCAGGCCCAAATGAACCGCATGATCTCATTGTATGATACTAAACGAAGTAACAAGCGATCCCTTGACCATGATGCCGGATCATCTAGTAGAGCCAGCAGCAGCACTGAAGAACCTCAACAAAGATTAGAAATATCAAAATCCAACGATCAGTCCAACAAACAAGAGCAAGAGACTAATTATGGTTGTGCTACAGACAAAAGATTCTTGATTGAAATTGAACCACACACTTCAAGAGAGAGTTCCACTTCAACCACGATCTTTGATGATCAATTGGGAACAAACGTAGAGTCATTCCTTGAGGAACTATTTGAGCTAGGAGGCAAAGCACGTCGCAACGATTTAGGTTTCATTTTCCCTCTCCGAGCAATTTTGCCGTGGTTTAATCATCAATGCATTGGGAACGTATTCATTCCAGAAAAAGAAAGAATTGGGAGTTCAGTAAGCCTACCTCTTCCTCTAGAATTGTATAACAATAAGGAATGGGTAGGATTTGCTGTATATGTGGCCTTCACTCTGCCTCCAGGTGTTTCACTTGAAAAATCATGTTGCTTCAAGTCTGTATTGTATACTAGTGATGGACATTTTTATTGCCTAAAATTATCGGCGGCTTGTGAAGACAATTTTAGGGGAGCACATCGACTCCTTGTCATCCACATACCACGAGGGCGTTTTAGAAAACAGTTGAATGAGTGCGTGGGAATTCGTGCTGCTTTTGTCTCCTCAACTCCAACTGTGGAGGCTGAGATGTGTGGGATCCGTGTAGTATACGAGCAAGATTTGAAAGGGTTAATCCAAACAATTACCCAATGCACCATAAGAAATCCGCCTGCAGTTTACTATGGACTTGCTCATTCATTCGTGCCAGATGGAAGAGATTCTTTCATGTTAGCTATTATGGAGGATAATTTGATTGAAGCAGCTAAATCCAGCAAAAGGAAGCTTTCAACCTTGCAAGAGCCTTTAACAGATTCAAAGTTCCTCAGTGGTCAAAAAATTTACTTAGAGATAGAGGCTGGAACTCCATACATGCCACACAAGTTCAAAGATTCCATAGTTGACTGGAAAAAAAACCTTGAGCTTTTGCTTGAATGTTTCTTTACCCTTTCTGATCTATCTATTAATCTCACTCTTCACAGAAGCACAACCTCTTTCTCAAGGGATTTTAATCCTTGTTCTGGTATTTTCCTTCAAAGAGAAATTCCAGTGTGGTTTACACCACAATATCCCTCCGAGAGTATGGTGTTGGAACTTCCTCCAAATTTGGAAGATGATTTGAATTTCAATGGACTTGTTGTATGTGCTGCCTTTTCTGTCCATGCCTCTGAAGCTGACGTCCTCAAATATCTGAAATCAGGACTCCATCCCCAACTTACGTGCCATTTCCAAAATTTGAGTTCTGAATCTTCTGATCAATCTTATGGTCTTACAGCTCCCATGATTGATACCAGTCCTGAGAACAAATTCATGTGGTTATATCGCAGAGGATTCATTTGGCAGGAATACCTACCCCGTGACTTATTTAAGGATCTGCTGCATAAAGATTCTTCAGTCAAGGTTTCATTTAGGAGTGATTCTTCCCAAGTCTTGGAGGTACAGAAATGTAGCTTCCGCATCTTATACAAGCAAGATGTGGAAAAATTCAAGCAAATGTTAATAGAGTGCTTTGCCTCCTTCTTTGACAATTACGACATCATTCGAGCATATTTAGTACAGGATTCTGGAGATTACAGCCACTTAAATCATCATGCAGTTACGCCTTTTTGTCAGGGTGAGACTAGTGGAAAAGCCAATGATCAGCTGGAACTAGGACATGTACCTGTCCAATATCAGCCCCAAATGGACCGCATGATCTCTTTGTATGATACTGAAGGAAGTAACATGCAATCCCTTGACCATGATGCAGGATCATCTAGTAGAACTGGCAGCAGTACTGAAGAACCTCAAATATTAGAAAGACCAGAATCCAATGATCGGTCCGATGAACAAGTGCACAAAACTAATTCTGACCTTACACATGTAGCAGAATGTGGTTATGCTTCAGACAGTAGATTCTTGATTGAAATTGAACCACACACTTCAAGAGATAATTCAAGTTCAACCAAGATTGTTGGTGATCAATTGAGAACAAATGTAGAGTCATTCCTTGAAGAACTATTTGAGCTGGGAGGCAAAGCACGTCGCTATGATTTAGGTTTCATTTTCTCTCTTCGAGCAATTTTACCGTGGTTTAAACATCAAGGCATTGGGGACGTGATAGGTGTACCTCTTCCTCTAGAATTGTATAACAACAAGGAATGCATAGGATTTGCTCTATATGTGATCTTCAGTCTGCCCCCAGGTGCTTGGCTTAAAGAAGGTTCTTGTTTTAAAATTGAGTTTACTGAAGAGCCAAGGTATTGCCTAAACTTAGCGTCCGCTGGTGAAGACAATTTCAGGGGTTCACATCGACTCCTGGTTATTCACATACCACAAAGACATTTTGGAGAACAGTTGAACCAGTGGAGTACCATTCGTGCTTCTTTTGAATCCCTTATGCCAGGTGTGGAGGTTGAGATGTGTGGCATGCGTGTAGTATACCAGGAAGATTTGAAAGGGTTAATCCAAACAATTACCAACTGCACAATATGCAGTCCCCCTGCTTACTATGGACTTGCCCAACCATTTGTGCCGGGAAGGTGGTCTTATGTGTTCACACACATGAGGAATAGTTTGGTTGAAGCAGCTAAATCCAGCAAGAGGAAGCTTTCAACCTTGCAAGAGCCTTTAACAGATTTAAAACCCATCCAAGGAATTCAGTTACTGATACTTGCTGCAACTCCATTTACTTCACTCAAGAAAACAGAAACAGTAATCAAGTGGAAAAGAAACCTTAAGCTTTTGCTTCAACGTTTCTTAACCCTTTCTGGTCTATCAATAAGTCTCATTCTTAGAGGAGGCAGAATTTCTTTCTCAGAGGGTCGTAATCTTCATACTGGTATTTTCTTTCAAAAGGAAATTCCAGACTGGTTTACACCACATCACTCTGCCAGTATGGTGCCCGAACTTCCTCCAGATTTGGAAAATGATATGAATTTGATGGGTTTTGTTGTATGTGCTGCCTTTTCAGTCCACGCCTCTAAAGCTGATGTCCTCAAATATCTGGATTCGGGACACCATCCCGAACTTATGTGCCATTTCCAATCAAGTTCTGACTATTCTGGTGTAGTAGTTCGTAACATGGTTGGCATCAATCCGAAGAACAAATTCATGTGGTTTTATCGTAGTGGATTCATTTGGCTGCATTACGTGCCCCGTCACAATGTTGAGCATCTCTTCAATAAACTATCTGAACTAAAGGTTTCATTTGAGAGTGATTCTTCCCAAGTATTGGCAGTACAGAAATGTAGCTTCCGTTTCTTATACAAGCAAGATGTAGAAGGATTTAAGCAAACAATAACAGAGTGCTTTACCTCCTTCTTTGACAACGAGGATATCATTAAGCCATATATAGAAGAAGAGGACTCTGGACATAATAAGTGGCCAAATCATGGAGATGAGATGGGACCATCTAGTACAACTGGTGGCAACATTGTCATTGAGCAAACTCAGAATGAGAGATTAGATGTACCCATAACTGATGGGAAGGGGAAAGGCCCTTCTGATGTCTCCTCAGTTGAAGCAGATGAGAGAGTTTATTATGGAACTACACCTGTTTGTCAGGGTGAAACTAGTGGACAAGCCAGTCATCAACTGGAACTACTTACACATGTAGTAGAAGAAGAACATGGTTCTAGTACTTCTCCTCAACAGCTAATCCCATTCTTATCTGAAATTGAACCTCCCGCTTCAACAGGTTCAAGTTCAACCAAATTTGAAGGTGATCAGTTCAGAACTAGTGGAAAATGCAGTGAACAGCTGGAACTAGTTACACATGTAGTAGAAGAAGAACACGGTTCTAGTTGTCCTCCGGAGCTAATGATCCCATTCTTATTTGAAATTGAACCTTCCGCCTCAACAGATTCAAGTCCAACCAAATTTGAAGGTGATCAGTTCAGAACAAATGTGGAGACTTTGCTCGCAGTAATGTTTCGAGATAAGATCAAAGGATATGACTTTGAACATGGTTTTATTTTATCTCCGAGAGTTGGAGCAATTTTGCCATGGTTTAATCACCAAACCATTGGGAGCGAAGCACTAGTACTTCATCTTCCTCCGGATTTATACAACGATAAGAAATGGGCAGGGTTAGCTCTATATGTTGCTTTCACTGTGCCCCCAGGTTATACGGAAAACATTCTATGTCGCTGTTCTTTGTCTACCTCTGCAGGACATGCAGAAGCGATCACAATAGCATGCTCCCAAGGCAATTTTTCCGGATCACGACGACTCTGTATCGTTCATAAACCGCGTGCACGTTTTCCAGAACAGTGGAATACGTGTAGTTACATTTCTGCCTTCTTTGAAGTCCTTACTCCAGTTGTGGATGTTGAGATGTGCGGGGTCCATCTAGTATACGAGCAAGATCTGATAGGGATAATCCAAACAATTACCCACTGCACTATCAGGAGTCCGCCTGTTTACTATGGGATTGGATTTGCTAACACACCACGTCAGTCGCAAATGGGTCAGCCTTTTGCGGATATAAATATGGACACAAATTTGAATGAACTCGCTTCAAGGACGCCATCTACCGCTCAAGAGCCTGTAAAGACCCTTGTTGGTCAAGTAATTCATTTACGCATACATGGTGACACCCCATTCAAGAAAAAAGATCCAGTAATGGAGTGGAAAAAAAACCTTGAGTTTTTGCTTCAAGGTTTCTTTAGGTTTCCTCTATCTATAACCCTCACTCTTGGAGGAGACGTCATCTCAAGCCTAGGTGATTCTAATCCTTACTGTCTCTGCAGCTCAAGTATTATCCCTCAAAAGGACATTCCGAAGTGGTTCTGTGGGGTGAAATATCCCACAAATAAGTTGAAGACCACACTCCCTCCGAATTTGGAAAATGATGAGAACTGGTTGGGATTTGTTGTCTGTGCTGCGTTTTCAGTCCATGCGTCCCAAGCTGACGTCCTCAGTTATCTGGATTCAGAAAACCATCCGAAACTTACATGCCTTTTGCAGTGGGGTTCTGAAAGAGCTTTGAATCTCAAAGCTGGCAAGAAGACGAGCATTGTTCTCAAGAATGAATTCATGTGGTTATATCTTAGACGATTCATTTGGCTGCGATACATACCCCATCAGAAATTTAAGGATCGGTTACATATATCTGATCACCTAGAGGCTTCATTTTGGACTGATTCAATTTCTTCCAACGTCTTGAGGATACAGGAATGTAGCCTGCGTGTTCTATACAAGAAAGACTTGGAAGAATTTAAGCAAACAATAATAGAGTGCTTTACCTCCTTCTTTGACAACGAGGATGTCATTCGGCCGTATATAGAAGAGGATTCCAGACATAACAAGTGGCCAAGGCACGCAGATGACAGAGATTCTTATGGCACTACACCCGTTTGTCAGGGTGAACGAGGTTCTGCATCCAGTACTTCTCTGGAGCTAATGCCATTTTTCATTGAAACTGAACCTCCAGCTTCAACGGATTCAAGTTCAACCGAAGTTACACGGCAACTGAGAATGATAGAGTCATCGCTTGAACAACTATTTCAGGGGGGCAGCGCACGCCACTATAATTATGGCTTTATTTTATCTCCTAGAGTCAGAGCAGTTTTGCCGTGGTTTAGCCATCAAAGCATTGGGAACGAAGTTAAAATTAATGCCCCTCCGGATTTGTATAAAGATAATAAATGGGTAGGCTTTGCTCTATATGTTGCCTTTACTCAGTCTCCAGGTTCACATAGTTGCTCTATGTGTAGTTTGTCTGTCTTCCCAGGACATTCAGTACATTCCATAAACACATGCCTCGATGAAGACCAATTTTCCGGATCACGAAGACTTTGTTTCTTTCATATACCACGTGCGCGCTTCCCTGAGCAGTGTGATCAGTGTCCTGTCATTTTTGCTAGGTTTGATTTCTTTAGCCCAGGTTCGGAGGTTGAAATGTGTGGGATCCGTGTAGTATATGATGAAGATATAAAAGGGCTAGTCCAAACAATTACTCAGAGCACTATCAGGAGTCCTCCGCCTTTTTACTTCTCCCAAGATGAAAAAACAGAAATCTTTTCCCCGGGGAGGCTTTTGCTGCCCATACATATGGCCTTGAGTTTGGTTGCTTACTCCAAAGCCTGCATCCTGCCCACACGAAGCGCACGAAGTCCACTGGTTCCATCCATGAAATTTGGTAAAGTAAACTTGGTCATACATGGTGACACTCGATTCAGGAAAGATACAACCGAGTGGAAAAGAAACTTCGAGATTTTGCTTCAATCTTTCTTCAGGCTTGATCTGTCCGTAAAACTCATTCTTGGAGAAGGCACCATCGCTTTCTATCAGGATTGCTTCAACCATGCTTGTCTTTTCCCTCAAAAGAAGATTCCAGATTGGTTCAAAGAAAAATCTCTCACATCTACGTATGTGTACCCACTTCCCGATGATTTGGAAAGTGACAACAATTGGATGGGACTTGTTGTATGTGCTGCCTTTTCAGTACACGCTCTTCCAACTGACCTTGGCAATTATAAGGACTTAGAACTGAGTTCTGATGTTAAGTGCAATTTTCGGTGGAGTTCTAATCCTTATCGATTCATTTCTATCAATGAGGGTGTGTTCGGCGTCAGGCCAAACAACAAATTCATGTGGTTATCTCGTAGAGGATTCATTTGGCTGCAATTCATATCTGGTGAAATTTTTAAGTTTGCCATGGATAAACATCGTCGTCAACTAGAAGCTTCATTTGAGAGTGGTTCCCCAGCTTTGTCGGTACAGGAATGTAGCTACCGAATCTTATACAAGCAAGACTTGCAAGAATATAAGCAAACAATTATACAGTGCTTCGCCTCTGTCTTTGACAACTTGGATCACAGTCTACCATATAAGGAAGACGATTCTCGACATAACGAGGAGCCAATTCATGAAGATGATCAGATGGACTCTTTAGGAACTACAAGCTTTTCTGAGGACACCCCCCATGGAAGAGAAACAACAGTGGTTCTTCCTGAGAGTGAAACCAGTGGAAATTCCTTTAATCGTTTCAACAGCATACTGTTTGAGCCCATTTTGTACGACAACAAAACGGCAGTAATTTTCGCTGATTTTCGAGGATGGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

2695

Amino Acids

306.04

Weight (kDa)

5.78

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 97 - 159 1.2e-07 C-JID domain
C-JID PF20160 690 - 838 4.9e-06 C-JID domain
C-JID PF20160 1035 - 1156 6.9e-06 C-JID domain
C-JID PF20160 1289 - 1432 6.8e-07 C-JID domain
C-JID PF20160 1927 - 2076 4.4e-06 C-JID domain
C-JID PF20160 2445 - 2592 2.4e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 261, 656, 7494
AasI GACNNNNNNGTC 2 cut(s) 16, 4599
AatII GACGTC 3 cut(s) 2214, 5724, 5931
AccB1I GGYRCC 2 cut(s) 3913, 7289
AccB7I CCANNNNNTGG 3 cut(s) 2464, 4246, 4406
AccBSI CCGCTC 1 cut(s) 5552
AccI GTMKAC 6 cut(s) 1445, 1644, 3453, 5174, 5370, 7840
AccII CGCG 2 cut(s) 5266, 6838
AccIII TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
AclI AACGTT 1 cut(s) 3766
AcoI YGGCCR 4 cut(s) 3, 4397, 6290, 6326
AcvI CACGTG 1 cut(s) 6833
AcyI GRCGYC 5 cut(s) 2211, 5540, 5721, 5928, 7587
AflII CTTAAG 1 cut(s) 3749
AflIII ACRYGT 6 cut(s) 862, 2689, 2893, 4624, 4789, 5294
AhdI GACNNNNNGTC 2 cut(s) 590, 1523
AhlI ACTAGT 6 cut(s) 1448, 2654, 4586, 4751, 4780, 5053
AjiI CACGTC 4 cut(s) 1208, 3056, 3130, 5468
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
Alw21I GWGCWC 4 cut(s) 1513, 2871, 5272, 6983
Alw44I GTGCAC 2 cut(s) 2867, 5268
AlwNI CAGNNNCTG 1 cut(s) 3679
Ama87I CYCGRG 2 cut(s) 322, 7042
Aor13HI TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
AoxI GGCC 9 cut(s) 3, 882, 1383, 4397, 4510, 6290, 6326, 7071, 7592
ApaLI GTGCAC 2 cut(s) 2867, 5268
ArsI GACNNNNNNTTYG 6 cut(s) 1151, 1183, 2999, 3031, 6060, 6092
AseI ATTAAT 2 cut(s) 1998, 6630
Asp700I GAANNNNTTC 7 cut(s) 1283, 1753, 2339, 4121, 5151, 5801, 6047
AspA2I CCTAGG 1 cut(s) 5734
AspLEI GCGC 5 cut(s) 421, 6536, 6838, 6840, 7124
AspS9I GGNCC 7 cut(s) 518, 883, 2719, 2853, 4421, 4511, 5359
AsuC2I CCSGG 5 cut(s) 323, 324, 3549, 7043, 7044
AvaI CYCGRG 2 cut(s) 322, 7042
AvaII GGWCC 5 cut(s) 518, 2719, 2853, 4421, 5359
AvrII CCTAGG 1 cut(s) 5734
BaeGI GKGCMC 5 cut(s) 2871, 3918, 4170, 5133, 5272
BalI TGGCCA 2 cut(s) 4399, 6328
BamHI GGATCC 2 cut(s) 1632, 6924
BanI GGYRCC 2 cut(s) 3913, 7289
BanII GRGCYC 1 cut(s) 8028
BarI GAAGNNNNNNTAC 6 cut(s) 5040, 5049, 5072, 5081, 7455, 7487
BauI CACGAG 2 cut(s) 542, 1539
BbrPI CACGTG 1 cut(s) 6833
BbsI GAAGAC 9 cut(s) 148, 1500, 3309, 5843, 6029, 6733, 6792, 6814, 7860
Bbv12I GWGCWC 4 cut(s) 1513, 2871, 5272, 6983
BceAI ACGGC 6 cut(s) 7, 1237, 6277, 6497, 6574, 8064
BciVI GTATCC 1 cut(s) 6177
BcnI CCSGG 5 cut(s) 323, 324, 3549, 7043, 7044
BcuI ACTAGT 6 cut(s) 1448, 2654, 4586, 4751, 4780, 5053
BfmI CTRYAG 4 cut(s) 1068, 1707, 5181, 5761
BfrI CTTAAG 1 cut(s) 3749
BfuI GTATCC 1 cut(s) 6177
BglI GCCNNNNNGGC 1 cut(s) 692
BglII AGATCT 1 cut(s) 5380
BlnI CCTAGG 1 cut(s) 5734
BmcAI AGTACT 4 cut(s) 2809, 4654, 5058, 6403
Bme18I GGWCC 5 cut(s) 518, 2719, 2853, 4421, 5359
BmeRI GACNNNNNGTC 2 cut(s) 590, 1523
BmeT110I CYCGRG 2 cut(s) 322, 7042
BmgBI CACGTC 4 cut(s) 1208, 3056, 3130, 5468
BmgT120I GGNCC 7 cut(s) 518, 883, 2719, 2853, 4421, 4511, 5359
BmiI GGNNCC 9 cut(s) 1634, 3915, 4422, 5360, 6926, 7140, 7291, 7720, 7878
BmsI GCATC 8 cut(s) 465, 940, 2499, 2767, 4113, 4195, 6404, 7113
BpiI GAAGAC 9 cut(s) 148, 1500, 3309, 5843, 6029, 6733, 6792, 6814, 7860
BpmI CTGGAG 9 cut(s) 332, 732, 1383, 2619, 3912, 5316, 6429, 6431, 6687
Bpu10I CCTNAGC 2 cut(s) 465, 6846
BpuMI CCSGG 5 cut(s) 323, 324, 3549, 7043, 7044
Bsa29I ATCGAT 1 cut(s) 7555
BsaAI YACGTR 5 cut(s) 2253, 4165, 5295, 6833, 7386
BsaBI GATNNNNATC 3 cut(s) 2043, 5745, 7683
BsaHI GRCGYC 5 cut(s) 2211, 5540, 5721, 5928, 7587
BsaWI WCCGGW 5 cut(s) 4825, 5071, 5231, 6640, 6797
Bse8I GATNNNNATC 3 cut(s) 2043, 5745, 7683
BseAI TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
BseCI ATCGAT 1 cut(s) 7555
BseJI GATNNNNATC 3 cut(s) 2043, 5745, 7683
BsePI GCGCGC 1 cut(s) 6836
BseRI GAGGAG 8 cut(s) 279, 530, 1588, 3825, 4516, 4650, 5730, 7889
BseSI GKGCMC 5 cut(s) 2871, 3918, 4170, 5133, 5272
BseX3I CGGCCG 1 cut(s) 6290
BseYI CCCAGC 2 cut(s) 3040, 7723
BsgI GTGCAG 2 cut(s) 3481, 5398
Bsh1236I CGCG 2 cut(s) 5266, 6838
Bsh1285I CGRYCG 1 cut(s) 6293
BshFI GGCC 9 cut(s) 5, 884, 1385, 4399, 4512, 6292, 6328, 7073, 7594
BshNI GGYRCC 2 cut(s) 3913, 7289
BshVI ATCGAT 1 cut(s) 7555
BsiEI CGRYCG 1 cut(s) 6293
BsiHKAI GWGCWC 4 cut(s) 1513, 2871, 5272, 6983
BsiHKCI CYCGRG 2 cut(s) 322, 7042
BsiSI CCGG 9 cut(s) 323, 954, 3548, 4826, 5072, 5232, 6641, 6798, 7043
BslFI GGGAC 8 cut(s) 405, 519, 3140, 3495, 4041, 4434, 5902, 7449
BsmBI CGTCTC 2 cut(s) 2, 5712
BsmFI GGGAC 8 cut(s) 405, 519, 3140, 3495, 4041, 4434, 5902, 7449
BsmI GAATGC 1 cut(s) 3179
BsnI GGCC 9 cut(s) 5, 884, 1385, 4399, 4512, 6292, 6328, 7073, 7594
BsoBI CYCGRG 2 cut(s) 322, 7042
Bsp1286I GDGCHC 8 cut(s) 1513, 2871, 3918, 4170, 5133, 5272, 6983, 8028
Bsp13I TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
Bsp19I CCATGG 3 cut(s) 5018, 7674, 7942
BspANI GGCC 9 cut(s) 5, 884, 1385, 4399, 4512, 6292, 6328, 7073, 7594
BspDI ATCGAT 1 cut(s) 7555
BspEI TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
BspFNI CGCG 2 cut(s) 5266, 6838
BspHI TCATGA 3 cut(s) 523, 562, 7885
BspLI GGNNCC 9 cut(s) 1634, 3915, 4422, 5360, 6926, 7140, 7291, 7720, 7878
BspMAI CTGCAG 3 cut(s) 1711, 5185, 5765
BspQI GCTCTTC 2 cut(s) 3258, 7476
BspT107I GGYRCC 2 cut(s) 3913, 7289
BspTI CTTAAG 1 cut(s) 3749
BsrBI CCGCTC 1 cut(s) 5552
BssHII GCGCGC 1 cut(s) 6836
BssNAI GTATAC 4 cut(s) 1446, 1645, 3454, 5371
BssNI GRCGYC 5 cut(s) 2211, 5540, 5721, 5928, 7587
BssSI CACGAG 2 cut(s) 542, 1539
BssT1I CCWWGG 9 cut(s) 3269, 3664, 5018, 5218, 5734, 6329, 7483, 7674, 7942
Bst1107I GTATAC 4 cut(s) 1446, 1645, 3454, 5371
Bst2BI CACGAG 2 cut(s) 542, 1539
BstACI GRCGYC 5 cut(s) 2211, 5540, 5721, 5928, 7587
BstAFI CTTAAG 1 cut(s) 3749
BstAPI GCANNNNNTGC 4 cut(s) 3504, 4165, 5189, 7774
BstBAI YACGTR 5 cut(s) 2253, 4165, 5295, 6833, 7386
BstDSI CCRYGG 6 cut(s) 1253, 3101, 5018, 6590, 7674, 7942
BstEII GGTNACC 1 cut(s) 317
BstFNI CGCG 2 cut(s) 5266, 6838
BstHHI GCGC 5 cut(s) 421, 6536, 6838, 6840, 7124
BstMCI CGRYCG 1 cut(s) 6293
BstPI GGTNACC 1 cut(s) 317
BstSFI CTRYAG 4 cut(s) 1068, 1707, 5181, 5761
BstSLI GKGCMC 5 cut(s) 2871, 3918, 4170, 5133, 5272
BstSNI TACGTA 1 cut(s) 7386
BstUI CGCG 2 cut(s) 5266, 6838
BstV2I GAAGAC 9 cut(s) 148, 1500, 3309, 5843, 6029, 6733, 6792, 6814, 7860
BstX2I RGATCY 6 cut(s) 575, 1632, 2404, 5380, 5629, 6924
BstXI CCANNNNNNTGG 4 cut(s) 3222, 5039, 5286, 7069
BstYI RGATCY 6 cut(s) 575, 1632, 2404, 5380, 5629, 6924
BstZ17I GTATAC 4 cut(s) 1446, 1645, 3454, 5371
BstZI CGGCCG 1 cut(s) 6290
Bsu15I ATCGAT 1 cut(s) 7555
BsuI GTATCC 1 cut(s) 6177
BsuRI GGCC 9 cut(s) 5, 884, 1385, 4399, 4512, 6292, 6328, 7073, 7594
BsuTUI ATCGAT 1 cut(s) 7555
BtgI CCRYGG 6 cut(s) 1253, 3101, 5018, 6590, 7674, 7942
BtgZI GCGATG 2 cut(s) 6489, 7279
BtrI CACGTC 4 cut(s) 1208, 3056, 3130, 5468
BtsI GCAGTG 4 cut(s) 4771, 5410, 5987, 6857
CaiI CAGNNNCTG 1 cut(s) 3679
CciI TCATGA 3 cut(s) 523, 562, 7885
CfoI GCGC 5 cut(s) 421, 6536, 6838, 6840, 7124
Cfr13I GGNCC 7 cut(s) 518, 883, 2719, 2853, 4421, 4511, 5359
Cfr9I CCCGGG 2 cut(s) 322, 7042
ClaI ATCGAT 1 cut(s) 7555
CpoI CGGWCCG 1 cut(s) 2853
CseI GACGC 4 cut(s) 3279, 5548, 5904, 7576
CspCI CAANNNNNGTGG 2 cut(s) 1120, 1155
CspI CGGWCCG 1 cut(s) 2853
DraI TTTAAA 4 cut(s) 232, 3109, 3247, 3654
DrdI GACNNNNNNGTC 2 cut(s) 16, 4599
DriI GACNNNNNGTC 2 cut(s) 590, 1523
DseDI GACNNNNNNGTC 2 cut(s) 16, 4599
EaeI YGGCCR 4 cut(s) 3, 4397, 6290, 6326
EagI CGGCCG 1 cut(s) 6290
Eam1105I GACNNNNNGTC 2 cut(s) 590, 1523
EciI GGCGGA 4 cut(s) 1692, 4855, 5418, 6987
EclXI CGGCCG 1 cut(s) 6290
Eco105I TACGTA 1 cut(s) 7386
Eco130I CCWWGG 9 cut(s) 3269, 3664, 5018, 5218, 5734, 6329, 7483, 7674, 7942
Eco24I GRGCYC 1 cut(s) 8028
Eco32I GATATC 1 cut(s) 4351
Eco47I GGWCC 5 cut(s) 518, 2719, 2853, 4421, 5359
Eco52I CGGCCG 1 cut(s) 6290
Eco72I CACGTG 1 cut(s) 6833
Eco88I CYCGRG 2 cut(s) 322, 7042
Eco91I GGTNACC 1 cut(s) 317
EcoO109I RGGNCCY 2 cut(s) 518, 4511
EcoO65I GGTNACC 1 cut(s) 317
EcoRI GAATTC 3 cut(s) 1578, 3669, 6047
EcoRV GATATC 1 cut(s) 4351
EcoT14I CCWWGG 9 cut(s) 3269, 3664, 5018, 5218, 5734, 6329, 7483, 7674, 7942
EcoT22I ATGCAT 3 cut(s) 199, 1274, 3179
EcoT38I GRGCYC 1 cut(s) 8028
ErhI CCWWGG 9 cut(s) 3269, 3664, 5018, 5218, 5734, 6329, 7483, 7674, 7942
Esp3I CGTCTC 2 cut(s) 2, 5712
FalI AAGNNNNNCTT 8 cut(s) 1741, 1773, 3544, 3576, 6206, 6238, 7757, 7789
FaqI GGGAC 8 cut(s) 405, 519, 3140, 3495, 4041, 4434, 5902, 7449
FauI CCCGC 2 cut(s) 4708, 5348
FauNDI CATATG 2 cut(s) 511, 7068
FblI GTMKAC 6 cut(s) 1445, 1644, 3453, 5174, 5370, 7840
FriOI GRGCYC 1 cut(s) 8028
GlaI GCGC 5 cut(s) 420, 6535, 6837, 6839, 7123
GsaI CCCAGC 2 cut(s) 3044, 7727
GsuI CTGGAG 9 cut(s) 332, 732, 1383, 2619, 3912, 5316, 6429, 6431, 6687
HaeIII GGCC 9 cut(s) 5, 884, 1385, 4399, 4512, 6292, 6328, 7073, 7594
HapII CCGG 9 cut(s) 323, 954, 3548, 4826, 5072, 5232, 6641, 6798, 7043
HgaI GACGC 4 cut(s) 3279, 5548, 5904, 7576
HhaI GCGC 5 cut(s) 421, 6536, 6838, 6840, 7124
Hin1I GRCGYC 5 cut(s) 2211, 5540, 5721, 5928, 7587
Hin6I GCGC 5 cut(s) 419, 6534, 6836, 6838, 7122
HinP1I GCGC 5 cut(s) 419, 6534, 6836, 6838, 7122
HincII GTYRAC 2 cut(s) 1936, 7694
HindII GTYRAC 2 cut(s) 1936, 7694
HindIII AAGCTT 5 cut(s) 1817, 3620, 3752, 7701, 7921
HpaII CCGG 9 cut(s) 323, 954, 3548, 4826, 5072, 5232, 6641, 6798, 7043
Hpy99I CGWCG 6 cut(s) 22, 28, 1212, 3060, 5244, 7692
Hsp92I GRCGYC 5 cut(s) 2211, 5540, 5721, 5928, 7587
HspAI GCGC 5 cut(s) 419, 6534, 6836, 6838, 7122
Kpn2I TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
LguI GCTCTTC 2 cut(s) 3258, 7476
LmnI GCTCC 9 cut(s) 424, 1508, 2314, 4828, 5006, 5043, 5219, 6412, 7876
LweI GCATC 8 cut(s) 465, 940, 2499, 2767, 4113, 4195, 6404, 7113
MbiI CCGCTC 1 cut(s) 5552
MfeI CAATTG 3 cut(s) 1148, 2996, 7426
MflI RGATCY 6 cut(s) 575, 1632, 2404, 5380, 5629, 6924
MhlI GDGCHC 8 cut(s) 1513, 2871, 3918, 4170, 5133, 5272, 6983, 8028
MlsI TGGCCA 2 cut(s) 4399, 6328
MluNI TGGCCA 2 cut(s) 4399, 6328
MmeI TCCRAC 9 cut(s) 553, 1044, 1056, 1631, 2103, 4910, 4984, 6195, 7499
Mox20I TGGCCA 2 cut(s) 4399, 6328
Mph1103I ATGCAT 3 cut(s) 199, 1274, 3179
MroI TCCGGA 5 cut(s) 4825, 5071, 5231, 6640, 6797
MroXI GAANNNNTTC 7 cut(s) 1283, 1753, 2339, 4121, 5151, 5801, 6047
MscI TGGCCA 2 cut(s) 4399, 6328
MslI CAYNNNNRTG 6 cut(s) 567, 3575, 4410, 6339, 7067, 7890
Msp20I TGGCCA 2 cut(s) 4399, 6328
MspA1I CMGCKG 3 cut(s) 2677, 3296, 4774
MspCI CTTAAG 1 cut(s) 3749
MspI CCGG 9 cut(s) 323, 954, 3548, 4826, 5072, 5232, 6641, 6798, 7043
MssI GTTTAAAC 1 cut(s) 3109
MunI CAATTG 3 cut(s) 1148, 2996, 7426
Mva1269I GAATGC 1 cut(s) 3179
MvnI CGCG 2 cut(s) 5266, 6838
NciI CCSGG 5 cut(s) 323, 324, 3549, 7043, 7044
NcoI CCATGG 3 cut(s) 5018, 7674, 7942
NdeI CATATG 2 cut(s) 511, 7068
NlaIV GGNNCC 9 cut(s) 1634, 3915, 4422, 5360, 6926, 7140, 7291, 7720, 7878
NmeAIII GCCGAG 2 cut(s) 31, 440
NmuCI GTSAC 7 cut(s) 317, 718, 2390, 4172, 5606, 7181, 7418
NsiI ATGCAT 3 cut(s) 199, 1274, 3179
PaeI GCATGC 2 cut(s) 3445, 5214
PagI TCATGA 3 cut(s) 523, 562, 7885
PauI GCGCGC 1 cut(s) 6836
PciI ACATGT 5 cut(s) 862, 2689, 2893, 4624, 4789
PciSI GCTCTTC 2 cut(s) 3258, 7476
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 640, 7123
PctI GAATGC 1 cut(s) 3179
PdmI GAANNNNTTC 7 cut(s) 1283, 1753, 2339, 4121, 5151, 5801, 6047
PflFI GACNNNGTC 1 cut(s) 13
PflMI CCANNNNNTGG 3 cut(s) 2464, 4246, 4406
PmaCI CACGTG 1 cut(s) 6833
PmeI GTTTAAAC 1 cut(s) 3109
PmlI CACGTG 1 cut(s) 6833
Ppu21I YACGTR 5 cut(s) 2253, 4165, 5295, 6833, 7386
PpuMI RGGWCCY 1 cut(s) 518
PscI ACATGT 5 cut(s) 862, 2689, 2893, 4624, 4789
PshBI ATTAAT 2 cut(s) 1998, 6630
PsiI TTATAA 3 cut(s) 261, 656, 7494
Psp1406I AACGTT 1 cut(s) 3766
Psp5II RGGWCCY 1 cut(s) 518
PspCI CACGTG 1 cut(s) 6833
PspEI GGTNACC 1 cut(s) 317
PspFI CCCAGC 2 cut(s) 3040, 7723
PspN4I GGNNCC 9 cut(s) 1634, 3915, 4422, 5360, 6926, 7140, 7291, 7720, 7878
PspPI GGNCC 7 cut(s) 518, 883, 2719, 2853, 4421, 4511, 5359
PspPPI RGGWCCY 1 cut(s) 518
PstI CTGCAG 3 cut(s) 1711, 5185, 5765
PstNI CAGNNNCTG 1 cut(s) 3679
PsuI RGATCY 6 cut(s) 575, 1632, 2404, 5380, 5629, 6924
PsyI GACNNNGTC 1 cut(s) 13
PteI GCGCGC 1 cut(s) 6836
PvuII CAGCTG 2 cut(s) 2677, 4774
RseI CAYNNNNRTG 6 cut(s) 567, 3575, 4410, 6339, 7067, 7890
Rsr2I CGGWCCG 1 cut(s) 2853
RsrII CGGWCCG 1 cut(s) 2853
SapI GCTCTTC 2 cut(s) 3258, 7476
Sau96I GGNCC 7 cut(s) 518, 883, 2719, 2853, 4421, 4511, 5359
ScaI AGTACT 4 cut(s) 2809, 4654, 5058, 6403
SduI GDGCHC 8 cut(s) 1513, 2871, 3918, 4170, 5133, 5272, 6983, 8028
SfaNI GCATC 8 cut(s) 465, 940, 2499, 2767, 4113, 4195, 6404, 7113
SfcI CTRYAG 4 cut(s) 1068, 1707, 5181, 5761
SinI GGWCC 5 cut(s) 518, 2719, 2853, 4421, 5359
SmaI CCCGGG 2 cut(s) 324, 7044
SmiMI CAYNNNNRTG 6 cut(s) 567, 3575, 4410, 6339, 7067, 7890
SnaBI TACGTA 1 cut(s) 7386
SpeI ACTAGT 6 cut(s) 1448, 2654, 4586, 4751, 4780, 5053
SphI GCATGC 2 cut(s) 3445, 5214
SspI AATATT 1 cut(s) 2826
StyI CCWWGG 9 cut(s) 3269, 3664, 5018, 5218, 5734, 6329, 7483, 7674, 7942
TaqII GACCGA 2 cut(s) 305, 2841
TatI WGTACW 9 cut(s) 2587, 2807, 4252, 4430, 4652, 5056, 6401, 6757, 7462
TauI GCSGC 2 cut(s) 53, 1487
TseFI GTSAC 7 cut(s) 317, 718, 2390, 4172, 5606, 7181, 7418
Tsp45I GTSAC 7 cut(s) 317, 718, 2390, 4172, 5606, 7181, 7418
TspGWI ACGGA 7 cut(s) 737, 1625, 4259, 5159, 6470, 6917, 7255
TspMI CCCGGG 2 cut(s) 322, 7042
Tth111I GACNNNGTC 1 cut(s) 13
Van91I CCANNNNNTGG 3 cut(s) 2464, 4246, 4406
Vha464I CTTAAG 1 cut(s) 3749
VneI GTGCAC 2 cut(s) 2867, 5268
VpaK11BI GGWCC 5 cut(s) 518, 2719, 2853, 4421, 5359
VspI ATTAAT 2 cut(s) 1998, 6630
XbaI TCTAGA 3 cut(s) 358, 1336, 3151
XcmI CCANNNNNNNNNTGG 6 cut(s) 2674, 4606, 5640, 6406, 6548, 6608
XmaI CCCGGG 2 cut(s) 322, 7042
XmaJI CCTAGG 1 cut(s) 5734
XmiI GTMKAC 6 cut(s) 1445, 1644, 3453, 5174, 5370, 7840
XmnI GAANNNNTTC 7 cut(s) 1283, 1753, 2339, 4121, 5151, 5801, 6047
ZraI GACGTC 3 cut(s) 2212, 5722, 5929
ZrmI AGTACT 4 cut(s) 2809, 4654, 5058, 6403
Zsp2I ATGCAT 3 cut(s) 199, 1274, 3179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.