Rmu_ssc0000183.1_g000044

Inter-alpha-trypsin inhibitor heavy

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000183.1
OG0010352
Physical Location & Seq
Forward (+)
215293 .. 224863
9571 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3099 bp
atggccgaggagttctccaaagccgtcgacgacggtctccggctctcgaaaagactatacttcgggaaggatcgagccgtggcgccgccgaggccgccgcctgccatggacaagtcggcgtcgtcggcgggttgcgcctacctcccgacgtctccgatggtgtacgccgtgatatcggacccgaggatcgtcgacaacccggacatcccgagctaccagccgcacgtgcacggcaggtgcgacccgccggcgctcattccgcttccgatgaacagggttgagctggaggtcgattgcttcatggacaccgcctttattcgggtcaccgggtcgtggagggtccattgcgtcaagagctccaggagctgtggctgccggattgccatccctatgggcgaacagggttcaattctaggtgttgaggtcgacatttctggtaaatcattctatactcagttaagtcaattgaaagatgacacggatatgagcaaggttccccaaggagaaggaggcttcctcaatgttaaacctcatatattcacattgacaactccgccggttgatgggggagtcaatctcacaattaaaatgagttggtctcagaagttattgtatcgaaatggagagttctctttagacataccgttcacatttccggagtttgtcattcctcccggaaagaagtacctgaaaaaggaaaagattgaattgaatgtgaactctggtctgggaactgaaattttattcaagggaactagtcatcttttgaaggaagtaaagagccaagaaggaaaattggggtttaaatacgagggaaatgttgtcaactggtctagtaccgacttccacttctcatatgcagtctcttcaagccaaatacatggcactgttatcacacagtcaccttccaaggatgatgttgatcaaagagagattttctctgcctatcttcttcctggaaaccagcgaagtggaaagggtttcagaaggaatgtagtcgttgttgttgatataagtggaagtatgcagggaaagccacttgaagatactcagaatgcactatctgaagccctctctaaacttgattcagaagattcattctgcattatagcttttaatggacagacttatgtttcatctacatcattgaaatcggctaccaaggaggcagttgaatcggccattgagtggattggcatcaactttattgctggtggtgatacaaatattatgcggccccttaacatggcaatagagatgctatccaactctaggggttcacttcctatcatatttctggttactgatggggcagttgaagatgagagacaaatatgcgatggaataaaaaagcgtcttgcaagtgaggatgcgatatcccctcgtatatacacttttggaataggttcattctgcaaccattatttcctgcggatgctatcaacaattggaaggggccaatatgatgctgcctatgatatagatttggttcagcctcgaatacagaacttatttgcgagagcttcatctgtgattcttacaaatataacccttgagacattggacgatcttgatgatgttgaggtgctcccttgccatattccagacctctcatttgagagtccattaagtgtatctggcagattccaaggcaagttacctgagaccttcaaggttaaaggattctcagctgatatgtcgaatattgtgatcgatttgaaagtacaagttgcaaaggacatacctcttcacagggtatatgcaaaaggagagattgagttactgactgctcaggcatggctgtcagagaacaagcagcttgaggataaggtagctaagttgagtgtacataccggtgctttgagcgagtatacacgcatggtcatatttgaaaaagaagtagctttgcaacagatggcctcaaagaaaagccagaacaaggaggatactgaaaccttgaaaatgatattgccacatagtttgtgcgtcggctttggaaacgtggccgcaacttcggacaacattcctccaggagccgaagaaccaaagcttccggaagcagttgaaattttcgccaaagccgcttcaggctgttgtggtctcattagtcaggtcgtcggattcgggattccgatgaacggtcgccagatttcggtcaccggtcaccggaatctgcagccggccactggtcacccgagtccgatattagagcttgcattattcatgaatggctattacgcaggaggtgagagaagagtagtagaattacttgaaaatcaatctggaaagatcctaaacaaagcattggtagaaggggaagatgagaattgcaggaaaccacactggtctcttcgcaacttcttcaggcgaccaactattattgggaaaaatttgctcactatagtgaaattcggtcttgtacaatatatgattctgaagacagtttgtgcattcctagcactcgtgttggaaatctttggcgtctatggtgatggggaattcaagtttcactacgggtatccatacatcacgatagtactgaatttcagccagatgtgggcattgtattgccttgtgcaattctataatgtgactcatgaaagacttcaaccaataaggccgcttgcaaagttcatcagctttaaggctattgtgtttgcgacttggtggcaaggtatggcaattgcagctgttgcacatgtgtttgtcttttcggcagaaccttaccattacattccatcttcttctgggtatgagaaaatcaccaccactgaaacaaccagtgaggaagcagtgaagttggaggaaggtgacagtagtacacaagctgtggttgaaacaaaagaaactcaggtcgaggctcccggaacaagtgtgactgagagtgttcaggacatagtgtttgaaggtggtcatcatgttgtcaaggatgttgtgttgaccataaatcaagcaatagggcctgtggagaagggtgtgacaaagacacaagaagccttccaccaaagatcggatgagcatgacgataagtcgaaggtttcagttgaggagagtattgaagtgactctttccgatgaagaaacacatttggaccgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_3

Protein Analysis

1032

Amino Acids

114.05

Weight (kDa)

5.57

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA PF00092 331 - 485 2.3e-12 von Willebrand factor type A domain
VWA_3 PF13768 331 - 489 1.7e-10 von Willebrand factor type A domain
VWA_2 PF13519 332 - 437 2.8e-11 von Willebrand factor type A domain
Solute_trans_a PF03619 754 - 909 8.7e-44 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 225
AatII GACGTC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 1188
AccI GTMKAC 4 cut(s) 27, 192, 426, 1871
AccIII TCCGGA 2 cut(s) 655, 2053
AclWI GGATC 3 cut(s) 78, 194, 2284
AcoI YGGCCR 4 cut(s) 3, 1179, 2004, 2181
AcsI RAATTY 6 cut(s) 738, 2067, 2389, 2408, 2498, 2542
AcuI CTGAAG 4 cut(s) 1086, 2070, 2347, 2456
AcvI CACGTG 1 cut(s) 226
AcyI GRCGYC 4 cut(s) 83, 119, 149, 2481
AfaI GTAC 8 cut(s) 164, 686, 838, 1725, 1848, 2421, 2538, 2821
AflIII ACRYGT 1 cut(s) 2698
AgeI ACCGGT 2 cut(s) 1853, 2159
AhdI GACNNNNNGTC 1 cut(s) 3028
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 3 cut(s) 359, 955, 2029
AleI CACNNNNGTG 1 cut(s) 2402
Alw21I GWGCWC 3 cut(s) 231, 359, 1590
Alw26I GTCTC 9 cut(s) 41, 156, 603, 868, 1321, 1550, 1658, 2105, 2352
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 3 cut(s) 78, 194, 2284
AlwNI CAGNNNCTG 1 cut(s) 366
Ama87I CYCGRG 3 cut(s) 181, 208, 2194
Aor13HI TCCGGA 2 cut(s) 655, 2053
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 5 cut(s) 372, 1469, 1816, 2176, 2687
ApoI RAATTY 6 cut(s) 738, 2067, 2389, 2408, 2498, 2542
ArsI GACNNNNNNTTYG 4 cut(s) 1598, 1630, 2885, 2917
AsiGI ACCGGT 2 cut(s) 1853, 2159
Asp700I GAANNNNTTC 2 cut(s) 1405, 2604
AspLEI GCGC 3 cut(s) 85, 137, 253
AspS9I GGNCC 6 cut(s) 178, 340, 1236, 1456, 2960, 3091
AsuC2I CCSGG 4 cut(s) 200, 328, 675, 2865
AvaI CYCGRG 3 cut(s) 181, 208, 2194
AvaII GGWCC 3 cut(s) 178, 340, 3091
BaeGI GKGCMC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 359
BauI CACGAG 1 cut(s) 2462
BbrPI CACGTG 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 2444
Bbv12I GWGCWC 3 cut(s) 231, 359, 1590
BbvI GCAGC 5 cut(s) 359, 1456, 1828, 2188, 2699
BccI CCATC 8 cut(s) 151, 392, 557, 1301, 1334, 1909, 2486, 2746
BceAI ACGGC 4 cut(s) 8, 62, 152, 247
BcgI CGANNNNNNTGC 2 cut(s) 1158, 1192
BciT130I CCWGG 3 cut(s) 361, 957, 2031
BciVI GTATCC 2 cut(s) 1939, 2529
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 4 cut(s) 200, 328, 675, 2865
BcoDI GTCTC 9 cut(s) 41, 156, 603, 868, 1321, 1550, 1658, 2105, 2352
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 5 cut(s) 413, 756, 834, 1272, 2456
BfmI CTRYAG 2 cut(s) 2174, 2400
BfoI RGCGCY 2 cut(s) 86, 254
BfuAI ACCTGC 1 cut(s) 225
BfuI GTATCC 2 cut(s) 1939, 2529
BglI GCCNNNNNGGC 1 cut(s) 91
BmcAI AGTACT 1 cut(s) 2538
Bme1390I CCNGG 7 cut(s) 200, 328, 361, 675, 957, 2031, 2865
Bme18I GGWCC 3 cut(s) 178, 340, 3091
BmeRI GACNNNNNGTC 1 cut(s) 3028
BmeT110I CYCGRG 3 cut(s) 181, 208, 2194
BmgT120I GGNCC 6 cut(s) 178, 340, 1236, 1456, 2960, 3091
BmiI GGNNCC 8 cut(s) 84, 180, 341, 495, 1238, 1457, 2035, 2862
BmrFI CCNGG 7 cut(s) 200, 328, 361, 675, 957, 2031, 2865
BmsI GCATC 5 cut(s) 1206, 1248, 1360, 1425, 1456
BoxI GACNNNNGTC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 2444
BplI GAGNNNNNCTC 9 cut(s) 31, 501, 533, 561, 593, 583, 615, 923, 955
BpmI CTGGAG 3 cut(s) 305, 343, 2013
Bpu10I CCTNAGC 1 cut(s) 1791
BpuEI CTTGAG 2 cut(s) 1574, 1841
BpuMI CCSGG 4 cut(s) 200, 328, 675, 2865
Bsa29I ATCGAT 1 cut(s) 1713
BsaAI YACGTR 1 cut(s) 226
BsaBI GATNNNNATC 3 cut(s) 383, 921, 1196
BsaHI GRCGYC 4 cut(s) 83, 119, 149, 2481
BsaI GGTCTC 5 cut(s) 41, 603, 1658, 2105, 2352
BsaJI CCNNGG 9 cut(s) 6, 78, 89, 105, 182, 499, 909, 1161, 1648
BsaWI WCCGGW 5 cut(s) 655, 1853, 2053, 2159, 2166
Bse118I RCCGGY 5 cut(s) 247, 556, 1853, 2159, 2179
Bse1I ACTGG 4 cut(s) 833, 2191, 2348, 2781
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 3 cut(s) 383, 921, 1196
BseAI TCCGGA 2 cut(s) 655, 2053
BseBI CCWGG 3 cut(s) 361, 957, 2031
BseCI ATCGAT 1 cut(s) 1713
BseDI CCNNGG 9 cut(s) 6, 78, 89, 105, 182, 499, 909, 1161, 1648
BseGI GGATG 7 cut(s) 204, 384, 919, 1375, 1440, 2935, 3019
BseJI GATNNNNATC 3 cut(s) 383, 921, 1196
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 8 cut(s) 467, 614, 1064, 1653, 1701, 1805, 2864, 2871
BseNI ACTGG 4 cut(s) 833, 2191, 2348, 2781
BseRI GAGGAG 2 cut(s) 23, 3062
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 5 cut(s) 359, 1456, 1828, 2188, 2699
Bsh1285I CGRYCG 1 cut(s) 2143
BshNI GGYRCC 1 cut(s) 82
BshTI ACCGGT 2 cut(s) 1853, 2159
BshVI ATCGAT 1 cut(s) 1713
BsiEI CGRYCG 1 cut(s) 2143
BsiHKAI GWGCWC 3 cut(s) 231, 359, 1590
BsiHKCI CYCGRG 3 cut(s) 181, 208, 2194
BsmAI GTCTC 9 cut(s) 41, 156, 603, 868, 1321, 1550, 1658, 2105, 2352
BsmBI CGTCTC 1 cut(s) 156
BsmI GAATGC 2 cut(s) 1060, 2450
Bso31I GGTCTC 5 cut(s) 41, 603, 1658, 2105, 2352
BsoBI CYCGRG 3 cut(s) 181, 208, 2194
Bsp1286I GDGCHC 3 cut(s) 231, 359, 1590
Bsp13I TCCGGA 2 cut(s) 655, 2053
Bsp1407I TGTACA 2 cut(s) 1846, 2419
Bsp143I GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
Bsp19I CCATGG 1 cut(s) 105
BspCNI CTCAG 8 cut(s) 466, 613, 1063, 1654, 1700, 1804, 2863, 2872
BspDI ATCGAT 1 cut(s) 1713
BspEI TCCGGA 2 cut(s) 655, 2053
BspHI TCATGA 2 cut(s) 2223, 2596
BspLI GGNNCC 8 cut(s) 84, 180, 341, 495, 1238, 1457, 2035, 2862
BspMAI CTGCAG 1 cut(s) 2178
BspMI ACCTGC 1 cut(s) 225
BspPI GGATC 3 cut(s) 78, 194, 2284
BspT107I GGYRCC 1 cut(s) 82
BspTNI GGTCTC 5 cut(s) 41, 603, 1658, 2105, 2352
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 5 cut(s) 247, 556, 1853, 2159, 2179
BsrGI TGTACA 2 cut(s) 1846, 2419
BsrI ACTGG 4 cut(s) 833, 2191, 2348, 2781
BssAI RCCGGY 5 cut(s) 247, 556, 1853, 2159, 2179
BssECI CCNNGG 9 cut(s) 6, 78, 89, 105, 182, 499, 909, 1161, 1648
BssMI GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
BssNAI GTATAC 1 cut(s) 1872
BssNI GRCGYC 4 cut(s) 83, 119, 149, 2481
BssSI CACGAG 1 cut(s) 2462
BssT1I CCWWGG 5 cut(s) 105, 499, 909, 1161, 1648
Bst1107I GTATAC 1 cut(s) 1872
Bst2BI CACGAG 1 cut(s) 2462
Bst2UI CCWGG 3 cut(s) 361, 957, 2031
Bst4CI ACNGT 8 cut(s) 35, 645, 889, 900, 2141, 2443, 2816, 3095
Bst6I CTCTTC 4 cut(s) 871, 1752, 2248, 2355
BstACI GRCGYC 4 cut(s) 83, 119, 149, 2481
BstAPI GCANNNNNTGC 1 cut(s) 2693
BstAUI TGTACA 2 cut(s) 1846, 2419
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 5 cut(s) 102, 249, 2181, 2214, 2625
BstDEI CTNAG 9 cut(s) 453, 600, 1050, 1662, 1687, 1791, 1836, 2850, 2880
BstDSI CCRYGG 2 cut(s) 78, 105
BstEII GGTNACC 4 cut(s) 322, 2155, 2162, 2189
BstENI CCTNNNNNAGG 1 cut(s) 692
BstF5I GGATG 7 cut(s) 204, 384, 919, 1375, 1440, 2935, 3019
BstH2I RGCGCY 2 cut(s) 86, 254
BstHHI GCGC 3 cut(s) 85, 137, 253
BstKTI GATC 7 cut(s) 73, 189, 925, 1570, 1713, 2292, 3011
BstMAI GTCTC 9 cut(s) 41, 156, 603, 868, 1321, 1550, 1658, 2105, 2352
BstMBI GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
BstMCI CGRYCG 1 cut(s) 2143
BstNI CCWGG 3 cut(s) 361, 957, 2031
BstNSI RCATGY 1 cut(s) 2702
BstPAI GACNNNNGTC 1 cut(s) 33
BstPI GGTNACC 4 cut(s) 322, 2155, 2162, 2189
BstSCI CCNGG 7 cut(s) 198, 326, 359, 673, 955, 2029, 2863
BstSFI CTRYAG 2 cut(s) 2174, 2400
BstSLI GKGCMC 1 cut(s) 231
BstV1I GCAGC 5 cut(s) 359, 1456, 1828, 2188, 2699
BstV2I GAAGAC 1 cut(s) 2444
BstX2I RGATCY 1 cut(s) 2289
BstXI CCANNNNNNTGG 3 cut(s) 391, 881, 971
BstYI RGATCY 1 cut(s) 2289
BstZ17I GTATAC 1 cut(s) 1872
Bsu15I ATCGAT 1 cut(s) 1713
BsuI GTATCC 2 cut(s) 1939, 2529
BsuTUI ATCGAT 1 cut(s) 1713
BtgI CCRYGG 2 cut(s) 78, 105
BtgZI GCGATG 1 cut(s) 1353
BtsCI GGATG 7 cut(s) 204, 384, 919, 1375, 1440, 2935, 3019
BtsI GCAGTG 1 cut(s) 2799
BtsIMutI CAGTG 6 cut(s) 885, 2184, 2341, 2769, 2788, 2799
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 5 cut(s) 102, 249, 2181, 2214, 2625
CaiI CAGNNNCTG 1 cut(s) 366
CciI TCATGA 2 cut(s) 2223, 2596
CfoI GCGC 3 cut(s) 85, 137, 253
Cfr10I RCCGGY 5 cut(s) 247, 556, 1853, 2159, 2179
Cfr13I GGNCC 6 cut(s) 178, 340, 1236, 1456, 2960, 3091
ClaI ATCGAT 1 cut(s) 1713
CseI GACGC 5 cut(s) 108, 337, 1343, 1975, 2470
Csp6I GTAC 8 cut(s) 163, 685, 837, 1724, 1847, 2420, 2537, 2820
CspAI ACCGGT 2 cut(s) 1853, 2159
CspCI CAANNNNNGTGG 2 cut(s) 1962, 1997
CviQI GTAC 8 cut(s) 163, 685, 837, 1724, 1847, 2420, 2537, 2820
DdeI CTNAG 9 cut(s) 453, 600, 1050, 1662, 1687, 1791, 1836, 2850, 2880
DinI GGCGCC 1 cut(s) 84
DpnI GATC 7 cut(s) 72, 188, 924, 1569, 1712, 2291, 3010
DpnII GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
DraI TTTAAA 1 cut(s) 805
DriI GACNNNNNGTC 1 cut(s) 3028
EaeI YGGCCR 4 cut(s) 3, 1179, 2004, 2181
Eam1104I CTCTTC 4 cut(s) 871, 1752, 2248, 2355
Eam1105I GACNNNNNGTC 1 cut(s) 3028
EarI CTCTTC 4 cut(s) 871, 1752, 2248, 2355
EciI GGCGGA 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 5 cut(s) 105, 499, 909, 1161, 1648
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 5 cut(s) 41, 603, 1658, 2105, 2352
Eco32I GATATC 2 cut(s) 174, 1377
Eco47I GGWCC 3 cut(s) 178, 340, 3091
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 4 cut(s) 1086, 2070, 2347, 2456
Eco72I CACGTG 1 cut(s) 226
Eco88I CYCGRG 3 cut(s) 181, 208, 2194
Eco91I GGTNACC 4 cut(s) 322, 2155, 2162, 2189
EcoICRI GAGCTC 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 2960
EcoO65I GGTNACC 4 cut(s) 322, 2155, 2162, 2189
EcoRI GAATTC 1 cut(s) 2498
EcoRII CCWGG 3 cut(s) 359, 955, 2029
EcoRV GATATC 2 cut(s) 174, 1377
EcoT14I CCWWGG 5 cut(s) 105, 499, 909, 1161, 1648
EcoT38I GRGCYC 1 cut(s) 359
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 5 cut(s) 105, 499, 909, 1161, 1648
Esp3I CGTCTC 1 cut(s) 156
FalI AAGNNNNNCTT 2 cut(s) 3051, 3083
FauI CCCGC 2 cut(s) 121, 252
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 4 cut(s) 27, 192, 426, 1871
FokI GGATG 7 cut(s) 191, 371, 926, 1382, 1447, 2942, 3026
FriOI GRGCYC 1 cut(s) 359
FspBI CTAG 5 cut(s) 413, 756, 834, 1272, 2456
GlaI GCGC 3 cut(s) 84, 136, 252
GsuI CTGGAG 3 cut(s) 305, 343, 2013
HaeII RGCGCY 2 cut(s) 86, 254
HgaI GACGC 5 cut(s) 108, 337, 1343, 1975, 2470
HhaI GCGC 3 cut(s) 85, 137, 253
Hin1I GRCGYC 4 cut(s) 83, 119, 149, 2481
Hin6I GCGC 3 cut(s) 83, 135, 251
HinP1I GCGC 3 cut(s) 83, 135, 251
HincII GTYRAC 5 cut(s) 28, 193, 427, 826, 2940
HindII GTYRAC 5 cut(s) 28, 193, 427, 826, 2940
HindIII AAGCTT 1 cut(s) 2048
Hpy99I CGWCG 9 cut(s) 29, 32, 35, 124, 127, 151, 194, 1991, 2120
HpyCH4III ACNGT 8 cut(s) 35, 645, 889, 900, 2141, 2443, 2816, 3095
HpyCH4IV ACGT 3 cut(s) 149, 225, 2001
HpyF3I CTNAG 9 cut(s) 453, 600, 1050, 1662, 1687, 1791, 1836, 2850, 2880
HpySE526I ACGT 3 cut(s) 149, 225, 2001
Hsp92I GRCGYC 4 cut(s) 83, 119, 149, 2481
HspAI GCGC 3 cut(s) 83, 135, 251
KasI GGCGCC 1 cut(s) 82
Kpn2I TCCGGA 2 cut(s) 655, 2053
KroI GCCGGC 2 cut(s) 247, 2179
KroNI GCCGGC 2 cut(s) 249, 2181
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
LmnI GCTCC 5 cut(s) 362, 363, 1593, 2033, 2866
Lsp1109I GCAGC 5 cut(s) 359, 1456, 1828, 2188, 2699
LweI GCATC 5 cut(s) 1206, 1248, 1360, 1425, 1456
MaeI CTAG 5 cut(s) 413, 756, 834, 1272, 2456
MaeII ACGT 3 cut(s) 149, 225, 2001
MalI GATC 7 cut(s) 72, 188, 924, 1569, 1712, 2291, 3010
MboI GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
MfeI CAATTG 3 cut(s) 464, 1446, 2682
MflI RGATCY 1 cut(s) 2289
MhlI GDGCHC 3 cut(s) 231, 359, 1590
Mly113I GGCGCC 1 cut(s) 83
MlyI GAGTC 5 cut(s) 579, 1630, 2206, 2587, 3058
MmeI TCCRAC 4 cut(s) 1290, 2098, 2448, 2781
MreI CGCCGGCG 1 cut(s) 247
MroI TCCGGA 2 cut(s) 655, 2053
MroNI GCCGGC 2 cut(s) 247, 2179
MroXI GAANNNNTTC 2 cut(s) 1405, 2604
MslI CAYNNNNRTG 4 cut(s) 389, 482, 1854, 2402
MspA1I CMGCKG 2 cut(s) 1691, 2690
MspR9I CCNGG 7 cut(s) 200, 328, 361, 675, 957, 2031, 2865
MunI CAATTG 3 cut(s) 464, 1446, 2682
Mva1269I GAATGC 2 cut(s) 1060, 2450
MvaI CCWGG 3 cut(s) 361, 957, 2031
NaeI GCCGGC 2 cut(s) 249, 2181
NarI GGCGCC 1 cut(s) 83
NciI CCSGG 4 cut(s) 200, 328, 675, 2865
NcoI CCATGG 1 cut(s) 105
NdeI CATATG 1 cut(s) 856
NdeII GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
NgoMIV GCCGGC 2 cut(s) 247, 2179
NlaIV GGNNCC 8 cut(s) 84, 180, 341, 495, 1238, 1457, 2035, 2862
NmeAIII GCCGAG 2 cut(s) 31, 114
NspI RCATGY 1 cut(s) 2702
OliI CACNNNNGTG 1 cut(s) 2402
PagI TCATGA 2 cut(s) 2223, 2596
PaqCI CACCTGC 1 cut(s) 225
PciI ACATGT 1 cut(s) 2698
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 2 cut(s) 1060, 2450
PdiI GCCGGC 2 cut(s) 249, 2181
PdmI GAANNNNTTC 2 cut(s) 1405, 2604
PflMI CCANNNNNTGG 1 cut(s) 1188
PfoI TCCNGGA 5 cut(s) 359, 673, 955, 2029, 2863
PinAI ACCGGT 2 cut(s) 1853, 2159
PleI GAGTC 5 cut(s) 578, 1629, 2205, 2587, 3058
PluTI GGCGCC 1 cut(s) 86
PmaCI CACGTG 1 cut(s) 226
PmlI CACGTG 1 cut(s) 226
PpsI GAGTC 5 cut(s) 578, 1629, 2205, 2587, 3058
Ppu21I YACGTR 1 cut(s) 226
PscI ACATGT 1 cut(s) 2698
PshAI GACNNNNGTC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 359, 955, 2029
PspCI CACGTG 1 cut(s) 226
PspEI GGTNACC 4 cut(s) 322, 2155, 2162, 2189
PspGI CCWGG 3 cut(s) 359, 955, 2029
PspN4I GGNNCC 8 cut(s) 84, 180, 341, 495, 1238, 1457, 2035, 2862
PspPI GGNCC 6 cut(s) 178, 340, 1236, 1456, 2960, 3091
PstI CTGCAG 1 cut(s) 2178
PstNI CAGNNNCTG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 2289
PvuII CAGCTG 2 cut(s) 1691, 2690
RsaI GTAC 8 cut(s) 164, 686, 838, 1725, 1848, 2421, 2538, 2821
RsaNI GTAC 8 cut(s) 163, 685, 837, 1724, 1847, 2420, 2537, 2820
RseI CAYNNNNRTG 4 cut(s) 389, 482, 1854, 2402
SacI GAGCTC 1 cut(s) 359
SalI GTCGAC 3 cut(s) 26, 191, 425
Sau3AI GATC 7 cut(s) 70, 186, 922, 1567, 1710, 2289, 3008
Sau96I GGNCC 6 cut(s) 178, 340, 1236, 1456, 2960, 3091
ScaI AGTACT 1 cut(s) 2538
SchI GAGTC 5 cut(s) 579, 1630, 2206, 2587, 3058
ScrFI CCNGG 7 cut(s) 200, 328, 361, 675, 957, 2031, 2865
SduI GDGCHC 3 cut(s) 231, 359, 1590
SfaNI GCATC 5 cut(s) 1206, 1248, 1360, 1425, 1456
SfcI CTRYAG 2 cut(s) 2174, 2400
SfoI GGCGCC 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 247
SgrDI CGTCGACG 1 cut(s) 26
SinI GGWCC 3 cut(s) 178, 340, 3091
SmiMI CAYNNNNRTG 4 cut(s) 389, 482, 1854, 2402
SmlI CTYRAG 2 cut(s) 1553, 1820
SmoI CTYRAG 2 cut(s) 1553, 1820
SpeI ACTAGT 1 cut(s) 755
SspDI GGCGCC 1 cut(s) 82
SspI AATATT 2 cut(s) 1228, 1705
SspMI CTAG 5 cut(s) 413, 756, 834, 1272, 2456
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 7 cut(s) 198, 326, 359, 673, 955, 2029, 2863
StyI CCWWGG 5 cut(s) 105, 499, 909, 1161, 1648
TaaI ACNGT 8 cut(s) 35, 645, 889, 900, 2141, 2443, 2816, 3095
TaiI ACGT 3 cut(s) 152, 228, 2004
TaqII GACCGA 2 cut(s) 2143, 2402
TatI WGTACW 5 cut(s) 1723, 1846, 2419, 2536, 2819
TauI GCSGC 8 cut(s) 88, 97, 100, 223, 1237, 2009, 2084, 2623
TscAI CASTG 6 cut(s) 892, 2191, 2348, 2776, 2788, 2799
TseI GCWGC 5 cut(s) 372, 1469, 1816, 2176, 2687
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 6 cut(s) 892, 2191, 2348, 2776, 2788, 2799
Van91I CCANNNNNTGG 1 cut(s) 1188
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 3 cut(s) 178, 340, 3091
XagI CCTNNNNNAGG 1 cut(s) 692
XapI RAATTY 6 cut(s) 738, 2067, 2389, 2408, 2498, 2542
XceI RCATGY 1 cut(s) 2702
XmiI GTMKAC 4 cut(s) 27, 192, 426, 1871
XmnI GAANNNNTTC 2 cut(s) 1405, 2604
XspI CTAG 5 cut(s) 413, 756, 834, 1272, 2456
ZraI GACGTC 1 cut(s) 150
ZrmI AGTACT 1 cut(s) 2538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.