Rmu_ssc0000189.1_g000028

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000189.1
OG0016581
Physical Location & Seq
Forward (+)
206337 .. 207287
951 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 951 bp
atgtatgatgatgatgatgatgatgatgatgatgacattgcagaattgataaagaaattgagtgcagtagttgatggggacatggtgttgaagtaccagttaatccctgaagatctggatgccttggtctctgttcgaagcgatgaagatgtaaagcacatgctggacgaatatgatagacaagaaaaccaaggcatgagtcctaggctaaggaccttcttgtttccatccaagccaatcatgatagaaaacagcagccactcattggagctagagcagcgttacattgacgccatcaatggcatagtccgttcatcgaaacaacagccaccctcaatcataggcagccctcacatcgccggcaccagcagcattgggaattgcagcagccccatagccagctacagcccccttgctagcttttcctctgcttgttcttcccccaagtcaggatcgcccgaacaccattttatggtggtacaagaagctaaccaaatattacaagccagtagattttcgcttcctcctgttatgcataaagtgcagagctctcccagcctttctggtaatcttaatcttcaacaataccacaacctaaactttgctcaccgtcaccaccagggtggtggtggtggataccattaccaacaaccccaaagtggccactaccaaatgtctagaagcctatcaagaaactcccacccccacaaccacagtagaggtggtctaggcagtcccaccccgccccatatgtctcccaggggaagatccgagtcagggagcgtgaggagcttgatgggtcagtctcgtcataattcgggttgcagtagcccgagacaacaaaaccaaagaccaaatgcaggagaaggcaacgattattgtgatgatccatatggctacaacagggttgatagagctgagagtctaccaacaagtcctcgacaatgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

316

Amino Acids

34.89

Weight (kDa)

6.07

Isoelectric Point (pI)

80.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 13 - 74 2.3e-07 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016581)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49920
fragaria_vesca FvH4_5g28590
malus_domestica MD15G1436100.v1.1
prunus_persica Prupe.1G579900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220591
rosa_laevigata RLG00000002215
rosa_multiflora Rmu_ssc0000189.1_g000028
rosa_roxburghii Rroxscaffold_3G00239280
rosa_rugosa Rorug07G0195200
rosa_samantha Rh7AG336200 Rh7BG327300 Rh7CG353900 Rh7DG332500
rosa_wichuraiana Rw7G028680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 2 cut(s) 265, 472
AccI GTMKAC 1 cut(s) 925
AciI CCGC 1 cut(s) 743
AclWI GGATC 3 cut(s) 460, 762, 881
AcoI YGGCCR 1 cut(s) 661
AcuI CTGAAG 1 cut(s) 129
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 2 cut(s) 95, 480
AfiI CCNNNNNNNGG 6 cut(s) 265, 449, 472, 659, 777, 860
AgsI TTSAA 2 cut(s) 91, 581
AjnI CCWGG 2 cut(s) 618, 758
AleI CACNNNNGTG 1 cut(s) 621
AluBI AGCT 7 cut(s) 271, 402, 420, 488, 549, 792, 917
AluI AGCT 7 cut(s) 271, 402, 420, 488, 549, 792, 917
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 4 cut(s) 133, 759, 810, 829
AlwI GGATC 3 cut(s) 460, 762, 881
Ama87I CYCGRG 1 cut(s) 832
AoxI GGCC 1 cut(s) 661
ApeKI GCWGC 6 cut(s) 255, 277, 345, 369, 384, 387
AspA2I CCTAGG 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 599, 605
AsuII TTCGAA 1 cut(s) 136
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 832
AvaII GGWCC 1 cut(s) 213
AvrII CCTAGG 1 cut(s) 203
BalI TGGCCA 1 cut(s) 663
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 551
Bbv12I GWGCWC 1 cut(s) 551
BbvI GCAGC 6 cut(s) 267, 289, 357, 381, 396, 399
BccI CCATC 4 cut(s) 68, 235, 302, 790
BciT130I CCWGG 2 cut(s) 620, 760
BciVI GTATCC 1 cut(s) 629
BcoDI GTCTC 4 cut(s) 133, 759, 810, 829
BfaI CTAG 5 cut(s) 204, 272, 417, 678, 728
BfmI CTRYAG 1 cut(s) 403
BfuI GTATCC 1 cut(s) 629
BglII AGATCT 1 cut(s) 112
BisI GCNGC 6 cut(s) 256, 278, 346, 370, 385, 388
BlnI CCTAGG 1 cut(s) 203
BlsI GCNGC 6 cut(s) 257, 279, 347, 371, 386, 389
Bme1390I CCNGG 2 cut(s) 620, 760
Bme18I GGWCC 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 832
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 2 cut(s) 620, 760
BmsI GCATC 1 cut(s) 109
BmtI GCTAGC 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 136
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 6 cut(s) 123, 190, 203, 619, 758, 759
Bsc4I CCNNNNNNNGG 6 cut(s) 265, 449, 472, 659, 777, 860
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 97, 507
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 2 cut(s) 620, 760
BseDI CCNNGG 6 cut(s) 123, 190, 203, 619, 758, 759
BseGI GGATG 2 cut(s) 124, 227
BseLI CCNNNNNNNGG 6 cut(s) 265, 449, 472, 659, 777, 860
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 2 cut(s) 97, 507
BseRI GAGGAG 1 cut(s) 802
BseXI GCAGC 6 cut(s) 267, 289, 357, 381, 396, 399
BseYI CCCAGC 1 cut(s) 554
BsgI GTGCAG 2 cut(s) 84, 563
BshFI GGCC 1 cut(s) 663
BshNI GGYRCC 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 551
BsiHKCI CYCGRG 1 cut(s) 832
BsiSI CCGG 1 cut(s) 360
BslFI GGGAC 2 cut(s) 92, 720
BslI CCNNNNNNNGG 6 cut(s) 265, 449, 472, 659, 777, 860
BsmAI GTCTC 4 cut(s) 133, 759, 810, 829
BsmFI GGGAC 2 cut(s) 92, 720
BsnI GGCC 1 cut(s) 663
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 832
Bsp119I TTCGAA 1 cut(s) 136
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 4 cut(s) 112, 452, 767, 886
BspACI CCGC 1 cut(s) 743
BspANI GGCC 1 cut(s) 663
BspCNI CTCAG 1 cut(s) 910
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 364
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 3 cut(s) 460, 762, 881
BspT104I TTCGAA 1 cut(s) 136
BspT107I GGYRCC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 2 cut(s) 97, 507
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 6 cut(s) 123, 190, 203, 619, 758, 759
BssMI GATC 4 cut(s) 112, 452, 767, 886
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 3 cut(s) 123, 190, 203
Bst2UI CCWGG 2 cut(s) 620, 760
Bst4CI ACNGT 2 cut(s) 611, 716
BstACI GRCGYC 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 541
BstBI TTCGAA 1 cut(s) 136
BstC8I GCNNGC 3 cut(s) 361, 400, 418
BstDEI CTNAG 2 cut(s) 209, 918
BstF5I GGATG 2 cut(s) 124, 227
BstKTI GATC 4 cut(s) 115, 455, 770, 889
BstMAI GTCTC 4 cut(s) 133, 759, 810, 829
BstMBI GATC 4 cut(s) 112, 452, 767, 886
BstMWI GCNNNNNNNGC 5 cut(s) 277, 369, 541, 555, 789
BstNI CCWGG 2 cut(s) 620, 760
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 618, 758
BstSFI CTRYAG 1 cut(s) 403
BstV1I GCAGC 6 cut(s) 267, 289, 357, 381, 396, 399
BstX2I RGATCY 2 cut(s) 112, 767
BstXI CCANNNNNNTGG 2 cut(s) 623, 626
BstYI RGATCY 2 cut(s) 112, 767
BsuI GTATCC 1 cut(s) 629
BsuRI GGCC 1 cut(s) 663
BtgZI GCGATG 2 cut(s) 156, 340
BtsCI GGATG 2 cut(s) 124, 227
Cac8I GCNNGC 3 cut(s) 361, 400, 418
CciI TCATGA 1 cut(s) 240
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 213
CseI GACGC 1 cut(s) 299
Csp6I GTAC 2 cut(s) 94, 479
CviAII CATG 4 cut(s) 82, 160, 196, 241
CviQI GTAC 2 cut(s) 94, 479
DdeI CTNAG 2 cut(s) 209, 918
DpnI GATC 4 cut(s) 114, 454, 769, 888
DpnII GATC 4 cut(s) 112, 452, 767, 886
EaeI YGGCCR 1 cut(s) 661
Ecl136II GAGCTC 1 cut(s) 549
Eco130I CCWWGG 3 cut(s) 123, 190, 203
Eco24I GRGCYC 1 cut(s) 551
Eco31I GGTCTC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 549
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 832
EcoICRI GAGCTC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 213
EcoRII CCWGG 2 cut(s) 618, 758
EcoT14I CCWWGG 3 cut(s) 123, 190, 203
EcoT22I ATGCAT 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 551
ErhI CCWWGG 3 cut(s) 123, 190, 203
FaeI CATG 4 cut(s) 85, 163, 199, 244
FaqI GGGAC 2 cut(s) 92, 720
FatI CATG 4 cut(s) 81, 159, 195, 240
FauI CCCGC 1 cut(s) 750
FauNDI CATATG 2 cut(s) 750, 892
FblI GTMKAC 1 cut(s) 925
Fnu4HI GCNGC 6 cut(s) 256, 278, 346, 370, 385, 388
FokI GGATG 2 cut(s) 131, 214
FriOI GRGCYC 1 cut(s) 551
Fsp4HI GCNGC 6 cut(s) 256, 278, 346, 370, 385, 388
FspBI CTAG 5 cut(s) 204, 272, 417, 678, 728
GluI GCNGC 6 cut(s) 256, 278, 346, 370, 385, 388
GsaI CCCAGC 1 cut(s) 558
HaeIII GGCC 1 cut(s) 663
HapII CCGG 1 cut(s) 360
HgaI GACGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 4 cut(s) 85, 163, 199, 244
HinfI GANTC 3 cut(s) 199, 773, 922
HpaII CCGG 1 cut(s) 360
HphI GGTGA 2 cut(s) 599, 605
Hpy166II GTNNAC 1 cut(s) 926
Hpy188I TCNGA 1 cut(s) 772
Hpy188III TCNNGA 5 cut(s) 116, 241, 450, 678, 690
Hpy8I GTNNAC 1 cut(s) 926
HpyAV CCTTC 2 cut(s) 226, 860
HpyCH4III ACNGT 2 cut(s) 611, 716
HpyCH4V TGCA 7 cut(s) 41, 65, 384, 535, 544, 825, 860
HpyF10VI GCNNNNNNNGC 5 cut(s) 277, 369, 541, 555, 789
HpyF3I CTNAG 2 cut(s) 209, 918
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 4 cut(s) 85, 163, 199, 244
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 4 cut(s) 112, 452, 767, 886
LmnI GCTCC 3 cut(s) 268, 780, 789
Lsp1109I GCAGC 6 cut(s) 267, 289, 357, 381, 396, 399
LweI GCATC 1 cut(s) 109
MaeI CTAG 5 cut(s) 204, 272, 417, 678, 728
MaeIII GTNAC 2 cut(s) 281, 611
MalI GATC 4 cut(s) 114, 454, 769, 888
MboI GATC 4 cut(s) 112, 452, 767, 886
MboII GAAGA 5 cut(s) 122, 158, 429, 569, 777
MflI RGATCY 2 cut(s) 112, 767
MhlI GDGCHC 1 cut(s) 551
MlsI TGGCCA 1 cut(s) 663
MluCI AATT 4 cut(s) 44, 56, 379, 814
MluNI TGGCCA 1 cut(s) 663
MlyI GAGTC 3 cut(s) 208, 782, 931
MnlI CCTC 7 cut(s) 343, 360, 436, 534, 713, 780, 948
Mox20I TGGCCA 1 cut(s) 663
Mph1103I ATGCAT 1 cut(s) 537
MroNI GCCGGC 1 cut(s) 359
MscI TGGCCA 1 cut(s) 663
MseI TTAA 2 cut(s) 101, 573
MslI CAYNNNNRTG 1 cut(s) 621
Msp20I TGGCCA 1 cut(s) 663
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 2 cut(s) 620, 760
MvaI CCWGG 2 cut(s) 620, 760
MwoI GCNNNNNNNGC 5 cut(s) 277, 369, 541, 555, 789
NaeI GCCGGC 1 cut(s) 361
NdeI CATATG 2 cut(s) 750, 892
NdeII GATC 4 cut(s) 112, 452, 767, 886
NgoMIV GCCGGC 1 cut(s) 359
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 4 cut(s) 85, 163, 199, 244
NlaIV GGNNCC 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 537
NspI RCATGY 1 cut(s) 163
NspV TTCGAA 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 621
PagI TCATGA 1 cut(s) 240
PasI CCCWGGG 1 cut(s) 759
PdiI GCCGGC 1 cut(s) 361
PflMI CCANNNNNTGG 2 cut(s) 265, 472
PkrI GCNGC 6 cut(s) 257, 279, 347, 371, 386, 389
PleI GAGTC 3 cut(s) 207, 781, 930
PpsI GAGTC 3 cut(s) 207, 781, 930
PpuMI RGGWCCY 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 618, 758
PspFI CCCAGC 1 cut(s) 554
PspGI CCWGG 2 cut(s) 618, 758
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 1 cut(s) 213
PspPPI RGGWCCY 1 cut(s) 213
PsuI RGATCY 2 cut(s) 112, 767
RsaI GTAC 2 cut(s) 95, 480
RsaNI GTAC 2 cut(s) 94, 479
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 1 cut(s) 551
SaqAI TTAA 2 cut(s) 101, 573
SatI GCNGC 6 cut(s) 256, 278, 346, 370, 385, 388
Sau3AI GATC 4 cut(s) 112, 452, 767, 886
Sau96I GGNCC 1 cut(s) 213
SchI GAGTC 3 cut(s) 208, 782, 931
ScrFI CCNGG 2 cut(s) 620, 760
SduI GDGCHC 1 cut(s) 551
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 403
SfuI TTCGAA 1 cut(s) 136
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 621
Sse9I AATT 4 cut(s) 44, 56, 379, 814
SsiI CCGC 1 cut(s) 743
SspI AATATT 1 cut(s) 498
SspMI CTAG 5 cut(s) 204, 272, 417, 678, 728
SstI GAGCTC 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 618, 758
StyI CCWWGG 3 cut(s) 123, 190, 203
TaaI ACNGT 2 cut(s) 611, 716
TaqI TCGA 3 cut(s) 136, 317, 940
TasI AATT 4 cut(s) 44, 56, 379, 814
Tru1I TTAA 2 cut(s) 101, 573
Tru9I TTAA 2 cut(s) 101, 573
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 6 cut(s) 255, 277, 345, 369, 384, 387
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 2 cut(s) 159, 303
TspGWI ACGGA 1 cut(s) 299
Van91I CCANNNNNTGG 2 cut(s) 265, 472
VpaK11BI GGWCC 1 cut(s) 213
XbaI TCTAGA 1 cut(s) 677
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 3 cut(s) 623, 626, 719
XmaJI CCTAGG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 925
XspI CTAG 5 cut(s) 204, 272, 417, 678, 728
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.