Rmu_ssc0000322.1_g000016

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000322.1
Physical Location & Seq
Reverse (-)
75125 .. 78318
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000322.1_g000016.1.cds

Sequence Viewer

Length: 843 bp
atgatgcttcattgtcgtggatcagatgaagctgtatcactgtatggttgtataatattgtggttgtgcttgatcaaagtggtgcaagtcaagtcgagagtactgttcatcacatgggttgcacgtgatgtcttgattgtctcctcaacctcaactgacacttttgggggtgcgtggacgagagccacatggctgcttatcgcaagcccttgcctcgtcggagatttgcctctcggatctcccacgagagtactgttcatcacatgggctgcacgtgatgtcttgattgtctcctcaacctcaactgacccttttgggggtgcgtggacgagagccacatggctgcttatcgcaagcccttgcctcgtcggagatttgcctctcggatctccaatacggcgtgccgtttcaggttcggcttcgtatatcgagagaattctctactacaccggaaagatccacgagattcatgaggtcagaggccgagatggattccatggattttttttggtttgggggggagaatggaattaccattcagtttactgcacttggaatgcttatcagattaacattattgaaacccctgatcacgttgatttcaccattgaggtcgagagagcgttgcgtgttcttgatggagccatccttgtcatttgtagcgttggtggtgtacagatcagtcagtcaattactgttgatgggcaaatgaaaaaatacgaggttccaagaattgcatttacaaacaaacttgatcgaatgggagcagatcaatggaaagttctgaatcaggtgccttatgtcaatctgttcatagcatatttccaaataccttatgtatga

Protein Analysis

280

Amino Acids

31.35

Weight (kDa)

6.99

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready

Orthologous Genes (Group: OG0019956)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0273791
rosa_multiflora Rmu_ssc0000322.1_g000016
rosa_roxburghii Rroxscaffold_6G00393050
rosa_samantha Rh5CG259900 Rh6AG192100 Rh6CG195600 Rh6DG187600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 793
AclWI GGATC 4 cut(s) 28, 244, 394, 451
AcsI RAATTY 1 cut(s) 435
AcvI CACGTG 2 cut(s) 125, 275
AfaI GTAC 3 cut(s) 102, 252, 675
AfiI CCNNNNNNNGG 2 cut(s) 316, 317
AgsI TTSAA 1 cut(s) 581
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 145, 295
AlwI GGATC 4 cut(s) 28, 244, 394, 451
AoxI GGCC 1 cut(s) 481
ApeKI GCWGC 3 cut(s) 193, 269, 343
ApoI RAATTY 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 595
BanI GGYRCC 1 cut(s) 793
BauI CACGAG 2 cut(s) 244, 461
BbrPI CACGTG 2 cut(s) 125, 275
BbvI GCAGC 3 cut(s) 180, 256, 330
BccI CCATC 4 cut(s) 482, 632, 653, 695
BceAI ACGGC 2 cut(s) 389, 413
BclI TGATCA 2 cut(s) 72, 589
BcoDI GTCTC 2 cut(s) 145, 295
BisI GCNGC 3 cut(s) 194, 270, 344
BlsI GCNGC 3 cut(s) 195, 271, 345
BmcAI AGTACT 2 cut(s) 102, 252
BmiI GGNNCC 3 cut(s) 643, 726, 795
BsaAI YACGTR 2 cut(s) 125, 275
BsaJI CCNNGG 1 cut(s) 496
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 317
BseDI CCNNGG 1 cut(s) 496
BseGI GGATG 1 cut(s) 645
BseLI CCNNNNNNNGG 2 cut(s) 316, 317
BseRI GAGGAG 2 cut(s) 133, 283
BseXI GCAGC 3 cut(s) 180, 256, 330
BsgI GTGCAG 2 cut(s) 255, 532
BshFI GGCC 1 cut(s) 483
BshNI GGYRCC 1 cut(s) 793
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 316, 317
BsmAI GTCTC 2 cut(s) 145, 295
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 1 cut(s) 483
Bsp1407I TGTACA 1 cut(s) 673
Bsp143I GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
Bsp19I CCATGG 1 cut(s) 496
BspANI GGCC 1 cut(s) 483
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 643, 726, 795
BspPI GGATC 4 cut(s) 28, 244, 394, 451
BspT107I GGYRCC 1 cut(s) 793
BsrGI TGTACA 1 cut(s) 673
BssECI CCNNGG 1 cut(s) 496
BssMI GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
BssSI CACGAG 2 cut(s) 244, 461
BssT1I CCWWGG 1 cut(s) 496
Bst2BI CACGAG 2 cut(s) 244, 461
Bst4CI ACNGT 4 cut(s) 42, 105, 255, 697
BstAUI TGTACA 1 cut(s) 673
BstBAI YACGTR 2 cut(s) 125, 275
BstC8I GCNNGC 3 cut(s) 205, 355, 402
BstDSI CCRYGG 1 cut(s) 496
BstF5I GGATG 1 cut(s) 645
BstKTI GATC 9 cut(s) 23, 75, 239, 389, 459, 592, 681, 757, 772
BstMAI GTCTC 2 cut(s) 145, 295
BstMBI GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
BstV1I GCAGC 3 cut(s) 180, 256, 330
BstX2I RGATCY 3 cut(s) 236, 386, 456
BstYI RGATCY 3 cut(s) 236, 386, 456
BsuRI GGCC 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 496
BtsCI GGATG 1 cut(s) 645
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 3 cut(s) 205, 355, 402
CciI TCATGA 1 cut(s) 469
Csp6I GTAC 3 cut(s) 101, 251, 674
CviAII CATG 6 cut(s) 114, 189, 264, 339, 470, 497
CviQI GTAC 3 cut(s) 101, 251, 674
DpnI GATC 9 cut(s) 22, 74, 238, 388, 458, 591, 680, 756, 771
DpnII GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
Eco130I CCWWGG 1 cut(s) 496
Eco72I CACGTG 2 cut(s) 125, 275
EcoRI GAATTC 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 6 cut(s) 117, 192, 267, 342, 473, 500
FatI CATG 6 cut(s) 113, 188, 263, 338, 469, 496
FbaI TGATCA 2 cut(s) 72, 589
Fnu4HI GCNGC 3 cut(s) 194, 270, 344
FokI GGATG 1 cut(s) 632
Fsp4HI GCNGC 3 cut(s) 194, 270, 344
GluI GCNGC 3 cut(s) 194, 270, 344
HaeIII GGCC 1 cut(s) 483
HapII CCGG 1 cut(s) 450
Hin1II CATG 6 cut(s) 117, 192, 267, 342, 473, 500
HinfI GANTC 3 cut(s) 466, 492, 787
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 595
Hpy166II GTNNAC 4 cut(s) 177, 327, 544, 674
Hpy188I TCNGA 8 cut(s) 25, 221, 236, 371, 386, 479, 567, 786
Hpy188III TCNNGA 7 cut(s) 96, 133, 283, 430, 470, 616, 635
Hpy8I GTNNAC 4 cut(s) 177, 327, 544, 674
Hpy99I CGWCG 2 cut(s) 221, 371
HpyCH4III ACNGT 4 cut(s) 42, 105, 255, 697
HpyCH4IV ACGT 3 cut(s) 124, 274, 594
HpyCH4V TGCA 5 cut(s) 85, 122, 272, 549, 737
HpySE526I ACGT 3 cut(s) 124, 274, 594
Hsp92II CATG 6 cut(s) 117, 192, 267, 342, 473, 500
Ksp22I TGATCA 2 cut(s) 72, 589
Kzo9I GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
LmnI GCTCC 2 cut(s) 641, 764
LpnPI CCDG 4 cut(s) 396, 463, 600, 776
Lsp1109I GCAGC 3 cut(s) 180, 256, 330
MaeII ACGT 3 cut(s) 124, 274, 594
MalI GATC 9 cut(s) 22, 74, 238, 388, 458, 591, 680, 756, 771
MboI GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
MflI RGATCY 3 cut(s) 236, 386, 456
MluCI AATT 4 cut(s) 435, 529, 690, 732
MmeI TCCRAC 2 cut(s) 199, 349
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 15
MspI CCGG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 562
NcoI CCATGG 1 cut(s) 496
NdeII GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
NlaIII CATG 6 cut(s) 117, 192, 267, 342, 473, 500
NlaIV GGNNCC 3 cut(s) 643, 726, 795
NmeAIII GCCGAG 1 cut(s) 509
PagI TCATGA 1 cut(s) 469
PctI GAATGC 1 cut(s) 562
PfeI GAWTC 3 cut(s) 466, 492, 787
PkrI GCNGC 3 cut(s) 195, 271, 345
PmaCI CACGTG 2 cut(s) 125, 275
PmlI CACGTG 2 cut(s) 125, 275
Ppu21I YACGTR 2 cut(s) 125, 275
PspCI CACGTG 2 cut(s) 125, 275
PspN4I GGNNCC 3 cut(s) 643, 726, 795
PsuI RGATCY 3 cut(s) 236, 386, 456
RsaI GTAC 3 cut(s) 102, 252, 675
RsaNI GTAC 3 cut(s) 101, 251, 674
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 3 cut(s) 194, 270, 344
Sau3AI GATC 9 cut(s) 20, 72, 236, 386, 456, 589, 678, 754, 769
ScaI AGTACT 2 cut(s) 102, 252
SmiMI CAYNNNNRTG 1 cut(s) 15
Sse9I AATT 4 cut(s) 435, 529, 690, 732
SspI AATATT 1 cut(s) 57
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 4 cut(s) 42, 105, 255, 697
TaiI ACGT 3 cut(s) 127, 277, 597
TaqI TCGA 4 cut(s) 95, 429, 615, 757
TasI AATT 4 cut(s) 435, 529, 690, 732
TatI WGTACW 3 cut(s) 100, 250, 673
TfiI GAWTC 3 cut(s) 466, 492, 787
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 45
TseI GCWGC 3 cut(s) 193, 269, 343
TspDTI ATGAA 6 cut(s) 42, 97, 247, 458, 725, 802
TspRI CASTG 1 cut(s) 45
XapI RAATTY 1 cut(s) 435
ZrmI AGTACT 2 cut(s) 102, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.