Rh5CG259900

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
Physical Location & Seq
Forward (+)
27582113 .. 27584578
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG259900.1

Sequence Viewer

Length: 345 bp
ATGGATTCCATGGATTTTTTTGGTTTGGGGGGAGAAAGGAATTACCATTCAGTTTACTGCACTTGGAATGCTTATCAGATTAACATTATTGAAACCCCTGATCACGTTGATTTCACCATTGAGGTCGAGAGAGCTTTGCGTGTTCTTGATGGAGCCATCCTTGTCATTTGTAGCGTTGGTGGTGTACAGATCAGTCAGTCAATTACTGTTGATAGGCAAATGAAAAAATACGAGGTTCCAAGAATTGTATTTACAAACAAACTTGATCGAATAGGAGCAGATCAATGGAAAGTTCTGAATCAGAGGAACTACATGCCGAATAGGAAGTTCATGGTTCCTGAATAG

Protein Analysis

114

Amino Acids

13.25

Weight (kDa)

6.1

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 16 - 101 2.1e-22 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Orthologous Genes (Group: OG0019956)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0273791
rosa_multiflora Rmu_ssc0000322.1_g000016
rosa_roxburghii Rroxscaffold_6G00393050
rosa_samantha Rh5CG259900 Rh6AG192100 Rh6CG195600 Rh6DG187600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 186
AgsI TTSAA 1 cut(s) 92
AluBI AGCT 1 cut(s) 134
AluI AGCT 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 106
BccI CCATC 2 cut(s) 143, 164
BclI TGATCA 1 cut(s) 100
BmiI GGNNCC 3 cut(s) 154, 237, 336
BsaJI CCNNGG 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 43
BsmI GAATGC 1 cut(s) 73
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 100, 189, 265, 280
Bsp19I CCATGG 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 154, 237, 336
BsrGI TGTACA 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 4 cut(s) 100, 189, 265, 280
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 208
BstAUI TGTACA 1 cut(s) 184
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 156
BstKTI GATC 4 cut(s) 103, 192, 268, 283
BstMBI GATC 4 cut(s) 100, 189, 265, 280
BstNSI RCATGY 1 cut(s) 316
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 156
Csp6I GTAC 1 cut(s) 185
CviAII CATG 3 cut(s) 10, 313, 331
CviJI RGCY 2 cut(s) 134, 155
CviKI_1 RGCY 2 cut(s) 134, 155
CviQI GTAC 1 cut(s) 185
DpnI GATC 4 cut(s) 102, 191, 267, 282
DpnII GATC 4 cut(s) 100, 189, 265, 280
Eco130I CCWWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 3 cut(s) 13, 316, 334
FaiI YATR 3 cut(s) 11, 314, 332
FatI CATG 3 cut(s) 9, 312, 330
FbaI TGATCA 1 cut(s) 100
FokI GGATG 1 cut(s) 143
Hin1II CATG 3 cut(s) 13, 316, 334
HinfI GANTC 2 cut(s) 5, 298
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 55, 185
Hpy188I TCNGA 3 cut(s) 78, 297, 303
Hpy188III TCNNGA 3 cut(s) 127, 146, 338
Hpy8I GTNNAC 2 cut(s) 55, 185
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 3 cut(s) 13, 316, 334
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 4 cut(s) 100, 189, 265, 280
LmnI GCTCC 2 cut(s) 152, 275
LpnPI CCDG 1 cut(s) 111
MaeII ACGT 1 cut(s) 105
MalI GATC 4 cut(s) 102, 191, 267, 282
MboI GATC 4 cut(s) 100, 189, 265, 280
MluCI AATT 3 cut(s) 40, 201, 243
MnlI CCTC 3 cut(s) 115, 226, 297
MseI TTAA 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 9
NdeII GATC 4 cut(s) 100, 189, 265, 280
NlaIII CATG 3 cut(s) 13, 316, 334
NlaIV GGNNCC 3 cut(s) 154, 237, 336
NspI RCATGY 1 cut(s) 316
PctI GAATGC 1 cut(s) 73
PfeI GAWTC 2 cut(s) 5, 298
PspN4I GGNNCC 3 cut(s) 154, 237, 336
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 81
Sau3AI GATC 4 cut(s) 100, 189, 265, 280
SetI ASST 4 cut(s) 108, 126, 136, 237
Sse9I AATT 3 cut(s) 40, 201, 243
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 126, 268
TasI AATT 3 cut(s) 40, 201, 243
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 2 cut(s) 5, 298
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TspDTI ATGAA 2 cut(s) 236, 319
XceI RCATGY 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.