Rh6CG195600

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
Physical Location & Seq
Reverse (-)
32409465 .. 32412217
2753 bp
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UTR
Exon/CDS
Intron
Rh6CG195600.1

Sequence Viewer

Length: 483 bp
ATGTGCTTGCCTCGTCGGAGATTTGCCTCTCGGATCTCCAATACGGCGTGCCGTTTCAGGGCTTCGAATATCGAGAGAATTCTCTACTACACCGGAAAGATCCACGAGGTCAGAGGCCGAGATGGCGTCGCTGCCAAAATGGATTCCATGGACTTTTTTGGTTTGGGGGGAGAAAGGAATTACCATTCAGTTTACTGCACTTGGAATGCTTATCAGATTAACATTATTGAAACCCCTGATCACGTTGATTTCACCATTGAGGTCGAGAGAGCTTTGCATGTTCTTGATGGAGCCATCCTTGTCATTTGTAGCGTTGGTGGTGTACAGATCAGTCAGTCAATTACTGTTGATAGGCAAAAGAAAAAATACGAGGTTCCAAGAATTGCATTTACAAACAAACTTGATCGAATAGGAGCAGATCAATGGAAAGTTCTGAATCAGAGGAACTACATGCCGAATAGGAAGTTCATGGTTCCTGAATAG

Protein Analysis

160

Amino Acids

18.52

Weight (kDa)

9.41

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 24 - 147 1.3e-22 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Orthologous Genes (Group: OG0019956)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0273791
rosa_multiflora Rmu_ssc0000322.1_g000016
rosa_roxburghii Rroxscaffold_6G00393050
rosa_samantha Rh5CG259900 Rh6AG192100 Rh6CG195600 Rh6DG187600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 41, 94
AcsI RAATTY 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 230
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
AlwI GGATC 2 cut(s) 41, 94
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 244
AsuII TTCGAA 1 cut(s) 65
BauI CACGAG 1 cut(s) 104
BbvI GCAGC 1 cut(s) 118
BccI CCATC 3 cut(s) 116, 281, 302
BceAI ACGGC 2 cut(s) 36, 60
BclI TGATCA 1 cut(s) 238
BglI GCCNNNNNGGC 1 cut(s) 123
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
BmiI GGNNCC 3 cut(s) 292, 375, 474
Bpu14I TTCGAA 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 118
BsgI GTGCAG 1 cut(s) 181
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 58
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 65
Bsp1407I TGTACA 1 cut(s) 322
Bsp143I GATC 6 cut(s) 33, 99, 238, 327, 403, 418
Bsp19I CCATGG 1 cut(s) 147
BspANI GGCC 1 cut(s) 117
BspLI GGNNCC 3 cut(s) 292, 375, 474
BspPI GGATC 2 cut(s) 41, 94
BspT104I TTCGAA 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 6 cut(s) 33, 99, 238, 327, 403, 418
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 346
BstACI GRCGYC 1 cut(s) 126
BstAUI TGTACA 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 2 cut(s) 8, 49
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 6 cut(s) 36, 102, 241, 330, 406, 421
BstMBI GATC 6 cut(s) 33, 99, 238, 327, 403, 418
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstNSI RCATGY 2 cut(s) 281, 454
BstV1I GCAGC 1 cut(s) 118
BstX2I RGATCY 2 cut(s) 33, 99
BstYI RGATCY 2 cut(s) 33, 99
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 8, 49
CseI GACGC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 323
CviAII CATG 4 cut(s) 148, 278, 451, 469
CviJI RGCY 4 cut(s) 62, 117, 272, 293
CviKI_1 RGCY 4 cut(s) 62, 117, 272, 293
CviQI GTAC 1 cut(s) 323
DpnI GATC 6 cut(s) 35, 101, 240, 329, 405, 420
DpnII GATC 6 cut(s) 33, 99, 238, 327, 403, 418
Eco130I CCWWGG 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 4 cut(s) 151, 281, 454, 472
FaiI YATR 4 cut(s) 149, 279, 452, 470
FatI CATG 4 cut(s) 147, 277, 450, 468
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 151, 281, 454, 472
HinfI GANTC 2 cut(s) 143, 436
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 2 cut(s) 193, 323
Hpy188I TCNGA 6 cut(s) 18, 33, 113, 216, 435, 441
Hpy188III TCNNGA 4 cut(s) 73, 265, 284, 476
Hpy8I GTNNAC 2 cut(s) 193, 323
Hpy99I CGWCG 2 cut(s) 18, 131
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 198, 277, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 151, 281, 454, 472
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 6 cut(s) 33, 99, 238, 327, 403, 418
LmnI GCTCC 2 cut(s) 290, 413
LpnPI CCDG 3 cut(s) 43, 106, 249
Lsp1109I GCAGC 1 cut(s) 118
MaeII ACGT 1 cut(s) 243
MalI GATC 6 cut(s) 35, 101, 240, 329, 405, 420
MboI GATC 6 cut(s) 33, 99, 238, 327, 403, 418
MflI RGATCY 2 cut(s) 33, 99
MluCI AATT 4 cut(s) 78, 178, 339, 381
MnlI CCTC 7 cut(s) 21, 37, 100, 107, 253, 364, 435
MseI TTAA 1 cut(s) 219
MspI CCGG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 123
NcoI CCATGG 1 cut(s) 147
NdeII GATC 6 cut(s) 33, 99, 238, 327, 403, 418
NlaIII CATG 4 cut(s) 151, 281, 454, 472
NlaIV GGNNCC 3 cut(s) 292, 375, 474
NmeAIII GCCGAG 1 cut(s) 143
NspI RCATGY 2 cut(s) 281, 454
NspV TTCGAA 1 cut(s) 65
PctI GAATGC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 143, 436
PkrI GCNGC 1 cut(s) 133
PspN4I GGNNCC 3 cut(s) 292, 375, 474
PsuI RGATCY 2 cut(s) 33, 99
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 6 cut(s) 33, 99, 238, 327, 403, 418
SetI ASST 5 cut(s) 111, 246, 264, 274, 375
SfuI TTCGAA 1 cut(s) 65
Sse9I AATT 4 cut(s) 78, 178, 339, 381
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 246
TaqI TCGA 4 cut(s) 65, 72, 264, 406
TasI AATT 4 cut(s) 78, 178, 339, 381
TatI WGTACW 1 cut(s) 322
TfiI GAWTC 2 cut(s) 143, 436
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseI GCWGC 1 cut(s) 131
TspDTI ATGAA 1 cut(s) 457
XapI RAATTY 1 cut(s) 78
XceI RCATGY 2 cut(s) 281, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.