Rmu_ssc0000473.1_g000002

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000473.1
OG0010730
Physical Location & Seq
Reverse (-)
16842 .. 33223
16382 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4998 bp
atggaagaaaacgacggtttcttatcgtctctgagcctccgtaactcctccgcgccaataccaagaccaaaccaaaacgatggtctcgacggcggcaaactcttcgtggactcttcaccaccttcatcggaagatgaaaatgacgccgtctccctccactccacttcaaaacgcctcgactatatgctccaattcctcgaccgcaagctctccgttgccaacgtcaacgtcaacgtcgtcgaccacatcaacaatatcaatatgagcgacgccgccgacactgctcagggctcctcctcgctccctgagttcgaggccaagggcggaggtaccggaatgttcaaagtcccggttcggggggcggtccacccgaaccggccgccaaggctggaactgaggccccaaccgctgagggagactcagataggatgcttcttgcggactatggtcagcaccgagtcccaattgtgggctgggacggagtgcgccgtcagggtttggaattttaaagatttgtactcgccggcggcggctgaggctggacgaggggaaggcgattcggaaacggcaccgtttagggagtccgtgtgcacatccgcggtgatgtgtttagttggggatgatgggaatagggtggtgtggagtggacatagggatggtaaaattaggtgttggaggatggactcctcctcctcagcactagtaactgctccttttaaggagggcttgtcttggcaggctcatagaggccccgttctttcacttgttatttcttgttacggggatctctggtcaggttcagagggtggtgtcatcaagatatggccatgggaagctattgaaaaagctctgtctcttaccattgaagaaaggcacatgtcttctttgttggtggagaggtcttacattgatccctggacccaagttgctgtcaatggtttcactaatgtattaacttcagatgttaggtatttattatctgatagctccagtgcaaaggtgtggagtgctggttatctctcattcgcactgtgggatgcgcgcacaagagaattactaaaagtgttcaataccgatggtctgaatgagaatcgagtggacatttcatcagcacaggacttgtcagtggaatttatttctggagcaaaaaaagacaaaacacaaagttcttttggtttctttcagcgatcacgtaatgctttaatgggagcagcagatgctgttcgtcgagttgcagtgaagggtgcctttggagatgataatcgaagaactgaagcattggtaattgcagtagatacaatgatttggacggggtgtgcaaatggcctacttgtgcagtgggatagaaatggcaaccgtatgcaagaattccattaccattcttctgctgtccaatgcttttgtacttttgggctacgtatatgggtgggttatgcaagtggcactgtccaggtattggacctcgacggtaatctgcttggaggatgggtagctgacaacagtcctattattaaaatagctacaggagctggttatgtatttaccttggccaatcatggtggtatatgtggatggaatattacatcaccgggacctcttgacagcatagtgcgttcagaattagctggcaaagaatttctatatacaaggatagaaaacttgaaaatattgactggtacatggaacgttggacaaggacgagcgtcacaggactcacttatatcctggctgggctctgtggcttctgatgttggcattgttgttgttggtttgcaagaagttgaaatgggtgctggttttcttgctatgtctgcagccaaagaaactgtaggtcttgaggggagctctgttgggcagtggtggctggatatgattggaaaaacattggatgaagggtcaacctttgaacgtgttggatccagacagttggcaggattgctcattgctatgtgggtgagacataatctcaaagctcatgtcggagatgtcgatgctgcagcagttccgtgtggttttgggcgcgctattggtaacaagggagctgttggtttgaggattagattgtatgacaggataatgtgctttgttaattgtcactttgctgcgcatttggaagctgttaatcgccgcaatgcagatttcgatcatgtatatcgaaccatgaccttctcccgacctaattttctcaattgtgcagctgctagtgcttcatctgctgttcaaatgcttcgcggtacaaatgctattggtaacaattctgtagaagggatgccggagttatctgaagcagacatggtcatatttcttggtgattttaactatcggcttgatggtatatcttatgatgaagctagagattttgtttctcaaagatgctttgattggcttagagaacgggatcagctccgaggggaaatggcagctggaaatgtctttcaaggaatgcgggaggcaaatattacatttcctcccacatacaagtttgaaaggcatcaagctggtttagcagggtatgattctggtgaaaagaagcgcattccagcttggtgtgatagaatcctgcatcgtgatagccgctcagcgttggtatcagaatgctgcttagaatgtcctgtagtctcttcaatatcacagtacgaggcttccatggatgtaactgacagcgatcacaagcctgtccgctgcatttttacccttgatattgctcgagttgatgagtcaataaggagacaagagcttggagatatccttgaatcaaatgggaaactcaaatgtatgcttgaagaactatgtaggattcctgaaacaattgtcagacaatctatcattaaggaggatggacatgcatcagatcattgtcccagaggttcctttggctttccgcgatggcttcaggtcactcctgcagctggcataatcagaccagatcatatagcagaggtgtctgttcatcatgaggaacaccaaaccctagaagagtttgttgatggtgtcccgcagaactgttggtgtgaaaacaccagagacaaggaagttatactggtggttaaggtacacgggaggtacagtaataacaccaaaagccatcgcgtctgtgtgcgccactgctgttcagccaagacaaagcaaagtgacccccccgagcacaacaccagacaaacccaaggagccgctcttcaccgttctaacttccaacaccttagcagttcctatgacgtggttgaccacctgtacgaggcttccatggatgtaactgacagcgatcacaagcctgtccgctgcatttttacccttgatattgctcgagttgatgagtcaataaggagacaagagcttggagatatccttgaatcaaatgggaaactcaaatgtatgcttgaagaactatgtaggattcctgaaacaattgtcagacaatctatcattaaggaggatggacatgcatcagatcattgtcccagaggttcctttggctttccgcgatggcttcaggtcactcctgcagctggcataatcagaccagatcatatagcagaggtgtctgttcatcatgaggaacaccaaaccctagaagagtttgttgatggtgtcccgcagaactgttggtgtgaaaacaccagagacaaggaagttatactggtggttaaggtacacgggaggtacagtaataacaccaaaagccatcgcgtctgtgtgcgtcactgctgttcagccaagacaaagcaaagtgacccccccgagcacaacaccagacaaacccaaggaaccgctcttcaccgttctaacttccaacaccttagcagttcctatgacgtgggggtgggtgatggtaagttggtgcggttcaaggtgaccgacgtaccacagtaccacgtgaatcagcagcgtgcgaatgtatccgctgctagcaccactgcatcaatcattgtttcaaaattgatcgatcctgtgttgatagtggtaacaatggaagcagagaacaaacaagtgatcttcaaggggttcatagagggcactccaaaggagacagacttacaagtgatggctgggattagtatcaagctcaaggctcctaaaggctctggagctttcctggtcaagaatctctacctctcttgtgacccttatatgaggggacgtatgcgtgattttcgtgattcatatcttcctccattcgttcctggcaagccacttgaaggatttggtgtctcccaagttgtggattctgataatccgagtttcaagcctggcgacttgatctctggcataaccggatgggaagaatacagcttgattcgtagaactgagcagttgagaaaaattaagcaagatgacatccctctatcgttccatttgggtcttcttggtatgccaggttttactgcatatgcaggattcaatgaggtctgctctcctaagaaaggggaatatgttttagtttctgctgcttctggagccgttggtcagcttgttggtcagcttgctaagttgcttggctgtcacgtagttggaagttctggttcaagccaaaaggttgatcttctgaggaataagcttggttttgatgatgcttttaactacaaggaagagccagacctcgatgcagctctgaaaaggtactttcctcaaggcattgacatctactttgataatgtgggaggggaaatgcttgatgcagcgcttctcaacatgagaattcatggtcgaattgctgtttgtgggatggtgtctgaaaaaaatctctctgatcccggagggatccacaatttatacaatctcataacaaagcgcatcaggatgcaaggatttctgcaaagtgattacttacacttgtacccgcgtttcttggaagatgttattagcctctacaagcaagggaagattgtttacgttgaagacatgaatgaaggcttggaaagtgctccatctgcttttgttgggttattttcgggaagaaatattggcaagcaagtcattcgtgtcgcaagatatgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

1665

Amino Acids

184.28

Weight (kDa)

6.1

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 137 - 525 2.6e-187 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 559 - 894 1.7e-65 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 559 - 890 1.3e-06 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 939 - 1036 2.3e-47 IP5P C-F Ig-like domain
Ig-like_IP5PC_F PF23755 1138 - 1235 2.3e-47 IP5P C-F Ig-like domain
ADH_N_2 PF16884 1328 - 1438 6.3e-24 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 1486 - 1607 6e-24 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 1519 - 1659 2.5e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2106
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 3 cut(s) 329, 568, 1244
AccB7I CCANNNNNTGG 2 cut(s) 1456, 4219
AccBSI CCGCTC 3 cut(s) 2577, 3173, 3770
AccI GTMKAC 1 cut(s) 240
AclI AACGTT 1 cut(s) 1686
AclWI GGATC 9 cut(s) 792, 905, 1911, 1924, 2406, 3938, 4742, 4753, 4766
AcoI YGGCCR 3 cut(s) 377, 824, 1548
AcsI RAATTY 5 cut(s) 502, 1130, 1367, 1634, 4695
AcuI CTGAAG 5 cut(s) 942, 1293, 2304, 2876, 3473
AcvI CACGTG 1 cut(s) 3874
AcyI GRCGYC 2 cut(s) 144, 270
AfeI AGCGCT 1 cut(s) 4680
AflIII ACRYGT 2 cut(s) 876, 1909
AhlI ACTAGT 1 cut(s) 700
AjiI CACGTC 2 cut(s) 3217, 3814
AjnI CCWGG 7 cut(s) 914, 1449, 1724, 4092, 4180, 4246, 4372
AjuI GAANNNNNNNTTGG 2 cut(s) 4895, 4927
Alw21I GWGCWC 5 cut(s) 593, 1847, 3147, 3744, 4924
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 9 cut(s) 792, 905, 1911, 1924, 2406, 3938, 4742, 4753, 4766
AlwNI CAGNNNCTG 2 cut(s) 1220, 1529
Ama87I CYCGRG 4 cut(s) 2706, 3140, 3303, 3737
Aor51HI AGCGCT 1 cut(s) 4680
AoxI GGCC 7 cut(s) 315, 377, 398, 748, 824, 1324, 1548
ApaLI GTGCAC 1 cut(s) 589
ApoI RAATTY 5 cut(s) 502, 1130, 1367, 1634, 4695
ArsI GACNNNNNNTTYG 6 cut(s) 67, 99, 1962, 1994, 4628, 4660
Asp718I GGTACC 1 cut(s) 329
AspS9I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
AsuC2I CCSGG 3 cut(s) 350, 1590, 4752
AsuNHI GCTAGC 1 cut(s) 3905
AvaI CYCGRG 4 cut(s) 2706, 3140, 3303, 3737
AvaII GGWCC 4 cut(s) 364, 918, 1459, 1592
BaeGI GKGCMC 2 cut(s) 593, 4016
BaeI ACNNNNGTAYC 6 cut(s) 3843, 3851, 3876, 3884, 4817, 4850
BalI TGGCCA 2 cut(s) 826, 1550
BamHI GGATCC 2 cut(s) 1916, 4758
BanI GGYRCC 3 cut(s) 329, 568, 1244
BanII GRGCYC 3 cut(s) 293, 1736, 1847
BarI GAAGNNNNNNTAC 2 cut(s) 4871, 4903
BbrPI CACGTG 1 cut(s) 3874
BbsI GAAGAC 3 cut(s) 873, 4352, 4902
Bbv12I GWGCWC 5 cut(s) 593, 1847, 3147, 3744, 4924
BbvCI CCTCAGC 3 cut(s) 410, 534, 694
BceAI ACGGC 5 cut(s) 106, 131, 473, 582, 4442
BcgI CGANNNNNNTGC 4 cut(s) 85, 119, 4958, 4992
BciT130I CCWGG 7 cut(s) 916, 1451, 1726, 4094, 4182, 4248, 4374
BciVI GTATCC 1 cut(s) 3907
BcnI CCSGG 3 cut(s) 350, 1590, 4752
BcuI ACTAGT 1 cut(s) 700
BfaI CTAG 6 cut(s) 701, 2202, 2352, 2972, 3569, 3906
BfmI CTRYAG 8 cut(s) 1521, 1812, 1827, 1995, 2259, 2613, 2904, 3501
BfoI RGCGCY 1 cut(s) 4682
BfuI GTATCC 1 cut(s) 3907
BglI GCCNNNNNGGC 1 cut(s) 385
BlpI GCTNAGC 1 cut(s) 2578
Bme18I GGWCC 4 cut(s) 364, 918, 1459, 1592
BmeT110I CYCGRG 4 cut(s) 2706, 3140, 3303, 3737
BmgBI CACGTC 2 cut(s) 3217, 3814
BmgT120I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
BmtI GCTAGC 1 cut(s) 3909
BoxI GACNNNNGTC 3 cut(s) 446, 1500, 1702
BpiI GAAGAC 3 cut(s) 873, 4352, 4902
BplI GAGNNNNNCTC 6 cut(s) 403, 435, 1829, 1861, 4394, 4426
BpmI CTGGAG 4 cut(s) 973, 1161, 4104, 4473
Bpu10I CCTNAGC 6 cut(s) 285, 410, 534, 694, 3200, 3797
Bpu1102I GCTNAGC 1 cut(s) 2578
BpuEI CTTGAG 3 cut(s) 1856, 4049, 4611
BpuMI CCSGG 3 cut(s) 350, 1590, 4752
Bsa29I ATCGAT 1 cut(s) 3942
BsaAI YACGTR 4 cut(s) 1193, 1418, 3874, 4504
BsaBI GATNNNNATC 4 cut(s) 1260, 2142, 4055, 4161
BsaHI GRCGYC 2 cut(s) 144, 270
BsaI GGTCTC 1 cut(s) 89
BsaWI WCCGGW 2 cut(s) 332, 4271
Bse118I RCCGGY 2 cut(s) 375, 523
Bse1I ACTGG 4 cut(s) 990, 1678, 3045, 3642
Bse3DI GCAATG 2 cut(s) 1941, 2137
Bse8I GATNNNNATC 4 cut(s) 1260, 2142, 4055, 4161
BseBI CCWGG 7 cut(s) 916, 1451, 1726, 4094, 4182, 4248, 4374
BseCI ATCGAT 1 cut(s) 3942
BseJI GATNNNNATC 4 cut(s) 1260, 2142, 4055, 4161
BseMI GCAATG 2 cut(s) 1941, 2137
BseNI ACTGG 4 cut(s) 990, 1678, 3045, 3642
BsePI GCGCGC 2 cut(s) 1040, 2020
BseRI GAGGAG 6 cut(s) 37, 283, 286, 676, 679, 682
BseSI GKGCMC 2 cut(s) 593, 4016
BseX3I CGGCCG 1 cut(s) 377
BseYI CCCAGC 3 cut(s) 473, 1729, 4046
BsgI GTGCAG 2 cut(s) 1355, 2214
Bsh1285I CGRYCG 2 cut(s) 202, 380
BshFI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
BshNI GGYRCC 3 cut(s) 329, 568, 1244
BshVI ATCGAT 1 cut(s) 3942
BsiEI CGRYCG 2 cut(s) 202, 380
BsiHKAI GWGCWC 5 cut(s) 593, 1847, 3147, 3744, 4924
BsiHKCI CYCGRG 4 cut(s) 2706, 3140, 3303, 3737
BsiSI CCGG 8 cut(s) 333, 350, 376, 524, 1589, 2273, 4272, 4752
BslFI GGGAC 9 cut(s) 332, 445, 490, 1605, 2844, 2978, 3441, 3575, 4149
BsmBI CGTCTC 2 cut(s) 33, 154
BsmFI GGGAC 9 cut(s) 332, 445, 490, 1605, 2844, 2978, 3441, 3575, 4149
BsmI GAATGC 3 cut(s) 2448, 2535, 2600
BsnI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 4 cut(s) 2706, 3140, 3303, 3737
Bsp1286I GDGCHC 8 cut(s) 293, 593, 1736, 1847, 3147, 3744, 4016, 4924
Bsp1720I GCTNAGC 1 cut(s) 2578
Bsp19I CCATGG 3 cut(s) 827, 2646, 3243
BspANI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
BspDI ATCGAT 1 cut(s) 3942
BspHI TCATGA 2 cut(s) 2953, 3550
BspMAI CTGCAG 4 cut(s) 1816, 1999, 2908, 3505
BspOI GCTAGC 1 cut(s) 3909
BspPI GGATC 9 cut(s) 792, 905, 1911, 1924, 2406, 3938, 4742, 4753, 4766
BspQI GCTCTTC 3 cut(s) 3180, 3777, 4581
BspT107I GGYRCC 3 cut(s) 329, 568, 1244
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 3 cut(s) 2577, 3173, 3770
BsrDI GCAATG 2 cut(s) 1941, 2137
BsrFI RCCGGY 2 cut(s) 375, 523
BsrI ACTGG 4 cut(s) 990, 1678, 3045, 3642
BssAI RCCGGY 2 cut(s) 375, 523
BssHII GCGCGC 2 cut(s) 1040, 2020
BssNI GRCGYC 2 cut(s) 144, 270
BssT1I CCWWGG 8 cut(s) 318, 383, 827, 1545, 2646, 3163, 3243, 3760
Bst2UI CCWGG 7 cut(s) 916, 1451, 1726, 4094, 4182, 4248, 4374
Bst6I CTCTTC 8 cut(s) 107, 118, 2626, 2970, 3180, 3567, 3777, 4581
BstACI GRCGYC 2 cut(s) 144, 270
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstBAI YACGTR 4 cut(s) 1193, 1418, 3874, 4504
BstDSI CCRYGG 4 cut(s) 597, 827, 2646, 3243
BstEII GGTNACC 1 cut(s) 3850
BstENI CCTNNNNNAGG 2 cut(s) 3233, 4419
BstH2I RGCGCY 1 cut(s) 4682
BstMCI CGRYCG 2 cut(s) 202, 380
BstNI CCWGG 7 cut(s) 916, 1451, 1726, 4094, 4182, 4248, 4374
BstNSI RCATGY 3 cut(s) 880, 2846, 3443
BstPAI GACNNNNGTC 3 cut(s) 446, 1500, 1702
BstPI GGTNACC 1 cut(s) 3850
BstSFI CTRYAG 8 cut(s) 1521, 1812, 1827, 1995, 2259, 2613, 2904, 3501
BstSLI GKGCMC 2 cut(s) 593, 4016
BstSNI TACGTA 1 cut(s) 1418
BstV2I GAAGAC 3 cut(s) 873, 4352, 4902
BstX2I RGATCY 3 cut(s) 784, 1916, 4758
BstXI CCANNNNNNTGG 2 cut(s) 80, 1927
BstYI RGATCY 3 cut(s) 784, 1916, 4758
BstZI CGGCCG 1 cut(s) 377
Bsu15I ATCGAT 1 cut(s) 3942
BsuI GTATCC 1 cut(s) 3907
BsuRI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
BsuTUI ATCGAT 1 cut(s) 3942
BtgI CCRYGG 4 cut(s) 597, 827, 2646, 3243
BtgZI GCGATG 4 cut(s) 2899, 3071, 3496, 3668
BtrI CACGTC 2 cut(s) 3217, 3814
BtsI GCAGTG 7 cut(s) 279, 1242, 1343, 1862, 3103, 3700, 3912
CaiI CAGNNNCTG 2 cut(s) 1220, 1529
CciI TCATGA 2 cut(s) 2953, 3550
Cfr10I RCCGGY 2 cut(s) 375, 523
Cfr13I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
Cfr42I CCGCGG 1 cut(s) 600
ClaI ATCGAT 1 cut(s) 3942
CseI GACGC 6 cut(s) 152, 278, 1692, 3079, 3676, 3686
CspCI CAANNNNNGTGG 2 cut(s) 1540, 1575
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 3 cut(s) 377, 824, 1548
EagI CGGCCG 1 cut(s) 377
Eam1104I CTCTTC 8 cut(s) 107, 118, 2626, 2970, 3180, 3567, 3777, 4581
EarI CTCTTC 8 cut(s) 107, 118, 2626, 2970, 3180, 3567, 3777, 4581
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 1845
EclXI CGGCCG 1 cut(s) 377
Eco105I TACGTA 1 cut(s) 1418
Eco130I CCWWGG 8 cut(s) 318, 383, 827, 1545, 2646, 3163, 3243, 3760
Eco24I GRGCYC 3 cut(s) 293, 1736, 1847
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 2 cut(s) 2746, 3343
Eco47I GGWCC 4 cut(s) 364, 918, 1459, 1592
Eco47III AGCGCT 1 cut(s) 4680
Eco52I CGGCCG 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 1845
Eco57I CTGAAG 5 cut(s) 942, 1293, 2304, 2876, 3473
Eco72I CACGTG 1 cut(s) 3874
Eco88I CYCGRG 4 cut(s) 2706, 3140, 3303, 3737
Eco91I GGTNACC 1 cut(s) 3850
EcoICRI GAGCTC 1 cut(s) 1845
EcoNI CCTNNNNNAGG 2 cut(s) 3233, 4419
EcoO109I RGGNCCY 3 cut(s) 399, 749, 1592
EcoO65I GGTNACC 1 cut(s) 3850
EcoRI GAATTC 2 cut(s) 1367, 4695
EcoRII CCWGG 7 cut(s) 914, 1449, 1724, 4092, 4180, 4246, 4372
EcoRV GATATC 2 cut(s) 2746, 3343
EcoT14I CCWWGG 8 cut(s) 318, 383, 827, 1545, 2646, 3163, 3243, 3760
EcoT22I ATGCAT 2 cut(s) 2848, 3445
EcoT38I GRGCYC 3 cut(s) 293, 1736, 1847
ErhI CCWWGG 8 cut(s) 318, 383, 827, 1545, 2646, 3163, 3243, 3760
Esp3I CGTCTC 2 cut(s) 33, 154
FalI AAGNNNNNCTT 4 cut(s) 710, 742, 1232, 1264
FaqI GGGAC 9 cut(s) 332, 445, 490, 1605, 2844, 2978, 3441, 3575, 4149
FauI CCCGC 4 cut(s) 2439, 3003, 3600, 4845
FauNDI CATATG 1 cut(s) 4387
FblI GTMKAC 1 cut(s) 240
FriOI GRGCYC 3 cut(s) 293, 1736, 1847
FspBI CTAG 6 cut(s) 701, 2202, 2352, 2972, 3569, 3906
FspI TGCGCA 1 cut(s) 2106
GsaI CCCAGC 3 cut(s) 477, 1733, 4050
GsuI CTGGAG 4 cut(s) 973, 1161, 4104, 4473
HaeII RGCGCY 1 cut(s) 4682
HaeIII GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
HapII CCGG 8 cut(s) 333, 350, 376, 524, 1589, 2273, 4272, 4752
HgaI GACGC 6 cut(s) 152, 278, 1692, 3079, 3676, 3686
Hin1I GRCGYC 2 cut(s) 144, 270
HincII GTYRAC 5 cut(s) 226, 232, 241, 1899, 3223
HindII GTYRAC 5 cut(s) 226, 232, 241, 1899, 3223
HindIII AAGCTT 1 cut(s) 4553
HpaII CCGG 8 cut(s) 333, 350, 376, 524, 1589, 2273, 4272, 4752
Hpy99I CGWCG 8 cut(s) 17, 92, 239, 242, 272, 1230, 1469, 3860
HpyAV CCTTC 8 cut(s) 132, 545, 1234, 1886, 2176, 2258, 4190, 4901
Hsp92I GRCGYC 2 cut(s) 144, 270
KpnI GGTACC 1 cut(s) 333
KroI GCCGGC 1 cut(s) 523
KroNI GCCGGC 1 cut(s) 525
KspI CCGCGG 1 cut(s) 600
LguI GCTCTTC 3 cut(s) 3180, 3777, 4581
MaeI CTAG 6 cut(s) 701, 2202, 2352, 2972, 3569, 3906
MbiI CCGCTC 3 cut(s) 2577, 3173, 3770
MfeI CAATTG 4 cut(s) 464, 2188, 2808, 3405
MflI RGATCY 3 cut(s) 784, 1916, 4758
MhlI GDGCHC 8 cut(s) 293, 593, 1736, 1847, 3147, 3744, 4016, 4924
MlsI TGGCCA 2 cut(s) 826, 1550
MluNI TGGCCA 2 cut(s) 826, 1550
MlyI GAGTC 8 cut(s) 104, 412, 467, 590, 677, 1706, 2726, 3323
MmeI TCCRAC 7 cut(s) 653, 1669, 1894, 1960, 3217, 3814, 4489
Mox20I TGGCCA 2 cut(s) 826, 1550
Mph1103I ATGCAT 2 cut(s) 2848, 3445
MreI CGCCGGCG 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 523
MscI TGGCCA 2 cut(s) 826, 1550
MslI CAYNNNNRTG 3 cut(s) 654, 1946, 4796
Msp20I TGGCCA 2 cut(s) 826, 1550
MspA1I CMGCKG 9 cut(s) 409, 599, 2198, 2423, 2682, 2909, 3279, 3506, 3902
MspI CCGG 8 cut(s) 333, 350, 376, 524, 1589, 2273, 4272, 4752
MunI CAATTG 4 cut(s) 464, 2188, 2808, 3405
Mva1269I GAATGC 3 cut(s) 2448, 2535, 2600
MvaI CCWGG 7 cut(s) 916, 1451, 1726, 4094, 4182, 4248, 4374
NaeI GCCGGC 1 cut(s) 525
NciI CCSGG 3 cut(s) 350, 1590, 4752
NcoI CCATGG 3 cut(s) 827, 2646, 3243
NdeI CATATG 1 cut(s) 4387
NgoMIV GCCGGC 1 cut(s) 523
NheI GCTAGC 1 cut(s) 3905
NsbI TGCGCA 1 cut(s) 2106
NsiI ATGCAT 2 cut(s) 2848, 3445
NspI RCATGY 3 cut(s) 880, 2846, 3443
PaeR7I CTCGAG 2 cut(s) 2706, 3303
PagI TCATGA 2 cut(s) 2953, 3550
PauI GCGCGC 2 cut(s) 1040, 2020
PciI ACATGT 1 cut(s) 876
PciSI GCTCTTC 3 cut(s) 3180, 3777, 4581
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 234, 1986
PctI GAATGC 3 cut(s) 2448, 2535, 2600
PdiI GCCGGC 1 cut(s) 525
PflFI GACNNNGTC 2 cut(s) 146, 2294
PflMI CCANNNNNTGG 2 cut(s) 1456, 4219
PfoI TCCNGGA 1 cut(s) 4750
PleI GAGTC 8 cut(s) 104, 412, 466, 589, 677, 1706, 2725, 3322
PmaCI CACGTG 1 cut(s) 3874
PmlI CACGTG 1 cut(s) 3874
PpsI GAGTC 8 cut(s) 104, 412, 466, 589, 677, 1706, 2725, 3322
Ppu21I YACGTR 4 cut(s) 1193, 1418, 3874, 4504
PpuMI RGGWCCY 1 cut(s) 1592
PscI ACATGT 1 cut(s) 876
PshAI GACNNNNGTC 3 cut(s) 446, 1500, 1702
Psp124BI GAGCTC 1 cut(s) 1847
Psp1406I AACGTT 1 cut(s) 1686
Psp5II RGGWCCY 1 cut(s) 1592
Psp6I CCWGG 7 cut(s) 914, 1449, 1724, 4092, 4180, 4246, 4372
PspCI CACGTG 1 cut(s) 3874
PspEI GGTNACC 1 cut(s) 3850
PspFI CCCAGC 3 cut(s) 473, 1729, 4046
PspGI CCWGG 7 cut(s) 914, 1449, 1724, 4092, 4180, 4246, 4372
PspPI GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
PspPPI RGGWCCY 1 cut(s) 1592
PspXI VCTCGAGB 2 cut(s) 2706, 3303
PstI CTGCAG 4 cut(s) 1816, 1999, 2908, 3505
PstNI CAGNNNCTG 2 cut(s) 1220, 1529
PsuI RGATCY 3 cut(s) 784, 1916, 4758
PsyI GACNNNGTC 2 cut(s) 146, 2294
PteI GCGCGC 2 cut(s) 1040, 2020
PvuII CAGCTG 4 cut(s) 2198, 2423, 2909, 3506
RseI CAYNNNNRTG 3 cut(s) 654, 1946, 4796
SacI GAGCTC 1 cut(s) 1847
SacII CCGCGG 1 cut(s) 600
SalI GTCGAC 1 cut(s) 239
SapI GCTCTTC 3 cut(s) 3180, 3777, 4581
Sau96I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
SchI GAGTC 8 cut(s) 104, 412, 467, 590, 677, 1706, 2726, 3323
SduI GDGCHC 8 cut(s) 293, 593, 1736, 1847, 3147, 3744, 4016, 4924
SfcI CTRYAG 8 cut(s) 1521, 1812, 1827, 1995, 2259, 2613, 2904, 3501
Sfr274I CTCGAG 2 cut(s) 2706, 3303
Sfr303I CCGCGG 1 cut(s) 600
SgrAI CRCCGGYG 1 cut(s) 523
SgrBI CCGCGG 1 cut(s) 600
SinI GGWCC 4 cut(s) 364, 918, 1459, 1592
SlaI CTCGAG 2 cut(s) 2706, 3303
SmiMI CAYNNNNRTG 3 cut(s) 654, 1946, 4796
SmlI CTYRAG 5 cut(s) 1835, 2706, 3303, 4064, 4626
SmoI CTYRAG 5 cut(s) 1835, 2706, 3303, 4064, 4626
SnaBI TACGTA 1 cut(s) 1418
SpeI ACTAGT 1 cut(s) 700
SspI AATATT 4 cut(s) 1579, 1668, 2458, 4960
SspMI CTAG 6 cut(s) 701, 2202, 2352, 2972, 3569, 3906
SstI GAGCTC 1 cut(s) 1847
StyI CCWWGG 8 cut(s) 318, 383, 827, 1545, 2646, 3163, 3243, 3760
TaqII GACCGA 1 cut(s) 3869
TatI WGTACW 2 cut(s) 516, 1403
TauI GCSGC 8 cut(s) 96, 275, 382, 530, 533, 2130, 2577, 3173
TspGWI ACGGA 5 cut(s) 29, 202, 494, 574, 1995
Tth111I GACNNNGTC 2 cut(s) 146, 2294
Van91I CCANNNNNTGG 2 cut(s) 1456, 4219
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 4 cut(s) 364, 918, 1459, 1592
XagI CCTNNNNNAGG 2 cut(s) 3233, 4419
XapI RAATTY 5 cut(s) 502, 1130, 1367, 1634, 4695
XceI RCATGY 3 cut(s) 880, 2846, 3443
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XhoI CTCGAG 2 cut(s) 2706, 3303
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 6 cut(s) 701, 2202, 2352, 2972, 3569, 3906
Zsp2I ATGCAT 2 cut(s) 2848, 3445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.