Rmu_ssc0000474.1_g000043

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000474.1
OG0014589
Physical Location & Seq
Reverse (-)
150081 .. 150536
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000474.1_g000043.1.cds

Sequence Viewer

Length: 456 bp
atgcatctcctttcccccaaagcccccctacaacctccaaaattccacaccagtcccttcccctccaccagacccaaaacccgaacaatccaatgcaaaaagaagagcataagcgatgccgagctcgcatcagatttgtcaaagatgaccacccttttcgcgcagagagacgatgccatgaacaagagcagagagcttctcttcacagagctgtgccaattcctggattcaaaaccagaggaagtcaagaagaaatggaggaaattggatgaggacgagaagcgggttttggtgaaagcctttgtttcagagtggggtgtcaattttcacccactgtctgcaaaatctgttcaagatttggtcgaggagcacctgcagcaccaaaacccacctgcaaaatcccctttgtttcctggtttaaagaggatgatgggtttctcagaagaagacaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

17.39

Weight (kDa)

9.23

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014589)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64480 AT5G64480
fragaria_vesca FvH4_2g11070
malus_domestica MD10G1057800.v1.1
prunus_persica Prupe.8G076600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0269271
rosa_laevigata RLG00000013863 RLG00000013866
rosa_multiflora Rmu_ssc0000474.1_g000042 Rmu_ssc0000474.1_g000043
rosa_roxburghii Rroxscaffold_7G00198890
rosa_rugosa Rorug06G0047300
rosa_samantha Rh6AG166800 Rh6BG172400 Rh6CG166200 Rh6DG157900
rosa_wichuraiana Rw6G014420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 381, 400
Acc36I ACCTGC 2 cut(s) 381, 400
AccB7I CCANNNNNTGG 1 cut(s) 223
AccII CGCG 1 cut(s) 161
AciI CCGC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 2 cut(s) 231, 353
AjnI CCWGG 2 cut(s) 222, 412
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 3 cut(s) 124, 196, 211
AluI AGCT 3 cut(s) 124, 196, 211
Alw21I GWGCWC 2 cut(s) 126, 372
Alw26I GTCTC 1 cut(s) 162
ApeKI GCWGC 1 cut(s) 376
ApoI RAATTY 1 cut(s) 41
AspLEI GCGC 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 304, 320
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 126, 372
BbvI GCAGC 1 cut(s) 388
BccI CCATC 1 cut(s) 424
BciT130I CCWGG 2 cut(s) 224, 414
BcoDI GTCTC 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 374
BfuAI ACCTGC 2 cut(s) 381, 400
BisI GCNGC 1 cut(s) 377
BlsI GCNGC 1 cut(s) 378
Bme1390I CCNGG 2 cut(s) 224, 414
BmrFI CCNGG 2 cut(s) 224, 414
BmsI GCATC 4 cut(s) 13, 106, 137, 163
BplI GAGNNNNNCTC 2 cut(s) 183, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 51
BseBI CCWGG 2 cut(s) 224, 414
BseGI GGATG 2 cut(s) 274, 432
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 1 cut(s) 388
Bsh1236I CGCG 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 126, 372
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 162
BsmBI CGTCTC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 126, 372
BspACI CCGC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 452
BspFNI CGCG 1 cut(s) 161
BspMAI CTGCAG 1 cut(s) 378
BspMI ACCTGC 2 cut(s) 381, 400
BspQI GCTCTTC 1 cut(s) 98
BsrI ACTGG 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 224, 414
Bst4CI ACNGT 1 cut(s) 336
Bst6I CTCTTC 2 cut(s) 98, 206
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 439
BstF5I GGATG 2 cut(s) 274, 432
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 163
BstMAI GTCTC 1 cut(s) 162
BstMWI GCNNNNNNNGC 2 cut(s) 125, 376
BstNI CCWGG 2 cut(s) 224, 414
BstSCI CCNGG 2 cut(s) 222, 412
BstSFI CTRYAG 1 cut(s) 374
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 2 cut(s) 274, 432
BtsIMutI CAGTG 1 cut(s) 332
BveI ACCTGC 2 cut(s) 381, 400
Cac8I GCNNGC 1 cut(s) 126
CfoI GCGC 1 cut(s) 163
CviAII CATG 1 cut(s) 178
CviJI RGCY 5 cut(s) 23, 124, 196, 211, 299
CviKI_1 RGCY 5 cut(s) 23, 124, 196, 211, 299
DdeI CTNAG 1 cut(s) 439
DraI TTTAAA 1 cut(s) 420
Eam1104I CTCTTC 2 cut(s) 98, 206
EarI CTCTTC 2 cut(s) 98, 206
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 222, 412
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 1 cut(s) 181
FaiI YATR 2 cut(s) 110, 179
FaqI GGGAC 1 cut(s) 39
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 377
FokI GGATG 2 cut(s) 281, 439
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 377
GlaI GCGC 1 cut(s) 162
GluI GCNGC 1 cut(s) 377
HhaI GCGC 1 cut(s) 163
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 1 cut(s) 227
HphI GGTGA 2 cut(s) 304, 320
Hpy188I TCNGA 3 cut(s) 133, 310, 442
Hpy188III TCNNGA 2 cut(s) 247, 353
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4V TGCA 5 cut(s) 4, 96, 341, 376, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 376
HpyF3I CTNAG 1 cut(s) 439
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 1 cut(s) 161
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 9 cut(s) 64, 82, 209, 236, 249, 386, 399, 405, 426
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 4 cut(s) 13, 106, 137, 163
MboII GAAGA 4 cut(s) 115, 193, 262, 455
MhlI GDGCHC 2 cut(s) 126, 372
MluCI AATT 4 cut(s) 41, 218, 263, 322
MnlI CCTC 7 cut(s) 45, 73, 232, 252, 265, 358, 417
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 224, 414
MvaI CCWGG 2 cut(s) 224, 414
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 125, 376
NlaIII CATG 1 cut(s) 181
NmeAIII GCCGAG 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 6
PaqCI CACCTGC 2 cut(s) 381, 400
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 222, 412
PspGI CCWGG 2 cut(s) 222, 412
PstI CTGCAG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 224, 414
SduI GDGCHC 2 cut(s) 126, 372
SetI ASST 6 cut(s) 37, 126, 198, 213, 375, 394
SfaNI GCATC 4 cut(s) 13, 106, 137, 163
SfcI CTRYAG 1 cut(s) 374
Sse9I AATT 4 cut(s) 41, 218, 263, 322
SsiI CCGC 1 cut(s) 283
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 222, 412
TaaI ACNGT 1 cut(s) 336
TaqI TCGA 1 cut(s) 363
TasI AATT 4 cut(s) 41, 218, 263, 322
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 1 cut(s) 339
TseI GCWGC 1 cut(s) 376
TspDTI ATGAA 1 cut(s) 194
TspRI CASTG 1 cut(s) 339
Van91I CCANNNNNTGG 1 cut(s) 223
XapI RAATTY 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.