Rroxscaffold_1G00013900

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007762
Physical Location & Seq
Reverse (-)
17110855 .. 17115913
5059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013900.1

Sequence Viewer

Length: 2610 bp
ATGCTTACATTCGTGAGCTTAGGTGCGATAAACAAGGGCATTGCAAATACTATCATGGATGGGCGTTTTGAGCCCACAAGGAATTTTGATGACCAACAATCTCTAGGAACAAGTGGAAACACATTGCGCCAAGCAAACCGAAGGTTGAATCTACAATCCAAGTTCAAATCAGAAAAGCCTAGTTTGTCCTATGCTGATCTCCATCATGCAATTACAAAAAATGTTAAAGATGTCTCACCCATGTCCGATGGGAACCACCAAAAGCAACGTATTAATAGAAAGACAACTGAAGTTGATGAACTTGTCAAGTATATGTCAAAGTTGCCAAGTTATCTTGAGAGAGGAAAAAACCTCCAGGAGAAAGCTTTAAATGTTGGAGTTCTTGACTGGGGGCGCCTTGAAAAATGGCAGTATAGCCATAAACAGATGCCATACAGAAGTAGCCGGTATTCACCATCCAGTAGTAATACAACCTCATGCTTTTCCACAGATGAATCATCAACCCATTCTAGCAGAGGTCACAGCTGTTCTCCTGCTCATCTTAGGATGCACCGTCCTTCCCTCCAATCTCATTTCATGACTTCTTCACTGGAAGGGCCTTCTGAAGTTGCCAACTCCTTTAGAGAAAGTGTTGGAAAGTTTCAGGATCTTAAAGCTGACCAGAGTAACAACCTGAATGGAGTCATTAGACCAGATAAGTCTTTCTGCAACAATCATATAGACATCAACGTGGCACAGTGCAAGAGAAAAGATTCAGACCCAAAGACTGAGCCAGAAAAAGGACATTTGCGGAATGGCTTGCAGTATGAGATGGCAGCAACAAATTTAAGGGTAAAGAAAAACACTCGAGATGGTGAGTTTCCAAAGAAAGTAGATAAGCAGCAGCCATTTTCAGAAAATTTTGAACTAGATACCCCTGAAGGTTGCGGTAAAGTTGTTCTGCTTTTGCCTAGAGATCTTCCTGAAAGAAATCATTCTGGGTCAGGGGTTTCTGACATTTCTGATTCGGAAACACTGCTTGGTCAAAGAGCTGCAGAAACAACTCGACGAAGCTTGCCAGAGAGACCCAAGGAGGCCTGTCTTGCAGAGCTCAATTCTGGTGTCCCCCACTCCTGCCGTTTTCCTTCTGAAGTTGATCGTAAACACTTCAGGGTAAAACAACTCGGCTCCACAGGTGCAGCAAGTGGCAGCTTTCAGTGTAATACACTTGGCTCAGCATCACAGTTGGCTCTAAGTTCCTCAACAAGAATGAGCCCATCCAGAGATAGAATTGTTGAAGACAAAAAAACAACGGGAGTATCAACATCTTCAACTGTAAATGAGCCTCATAGAGGATCAGAATTAAAGCGAGGTAAAGTAACTGCTGAAAAAGTGAGAAGCTCTTCACCTTTCCGCCGATTAAGCATTGCTGTGGGTAAGATGAGTAAAACTTCCGGTTCTAAAGATTCTTCAGATGTACAACAGCCTAGGTCAACAACTTTCCAGGCTAGACCTGGTTCGGGGAACAACGTGGCTTCCACTTTCTTGGATACTTCAGACACTGATAATTCTAATGCCACTAGCAGAGCCAGGTCCAGCCCTCTGAGGAGGTTACTGGATCCACTGCTGAAGCCAAAGATAGCCAACTGCCACCATTCAGCGGAGCCATTGGAGAAAGATTCCATATCAACAAACAAGGCTTGTAAATCATCTGAGGGCGGGTGGAATCTTTTCCACAATGACATTGACATTCTTGCTGCCACAATGAAGAAGTTGAATACCTCAGGAAAGGGTGACTGCAGTTGTATCTACACATTCTTCAGCGTGCGGGAAGTCAAGAAAAAGACTGGAACTTGGCTGAATCATGGAAGCAAAGGCAAAGCTCATGAATACATTCGTAATGTTGTTGCTCAAATGAAGGTTTCTGATTCACAGTTTCCCAATTTGATACTCGATCACCACTGTTGTCAAAATCCCGAAAAGAGGAGTCAGACATCATCTAGAGATTGGCGTCGTAGGGATTTCTATAGTGACTTGCCTGCGGTTGGTTCAGTAGAGTGTTTGTCAAAGGTGAGACGCCACTCCTATTCTGTGGAAGATGTACAGAGCAAGCCTTTTGTTGGTAGTGAAGGCCTCATTAGCACAACGGTCATACTTCCAAGTGGTGCTCATAGTCTTCCGAGTAATGGAGGACCATCATCTCTGATTGAACGTTGGAGTACAGGTGGCTCATGTGACTGTGGGGGTTGGGATTTGGGTTGCAAACTACGGATATTGGACAACCAGAATAAAGCCAGTGAGAATTTGACTTCACAGAAGGTTTGTTCAATCCCAGATCGGTTTGAGCTTTGCCATCAGGGAGGACTACAAGAAAACCAACCTGCTTTAAGTTTGTCCCCATTCAAGGATGGGATCTATTCAGTTGAGTTTAATTCTTCACTCTCAGTTTTACAAGCATTCTCCATTTGTATAGCAGTGTTAGATAGTAGGAATCTATGCGAGTTCTCAGAGTTCCGGGAAACCAAAGCAACAGTGCAAGATGATGGAATAAGTGATCTGAATCAAATGGAAGGGGAAGTTCCTGCAAGATATGCCTCGTATCCTCCTCTTTCACCAGCCGGGCGCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

869

Amino Acids

95.99

Weight (kDa)

9.11

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 689 - 822 2.2e-40 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2368
AccB1I GGYRCC 1 cut(s) 393
AccII CGCG 1 cut(s) 2604
AciI CCGC 7 cut(s) 790, 927, 1393, 1640, 1698, 1807, 2021
AclI AACGTT 1 cut(s) 2191
AclWI GGATC 5 cut(s) 654, 1342, 1592, 1605, 2399
AcsI RAATTY 4 cut(s) 82, 823, 898, 2281
AcuI CTGAAG 9 cut(s) 309, 624, 939, 1132, 1149, 1434, 1518, 1628, 1783
AcyI GRCGYC 3 cut(s) 394, 1990, 2056
AfaI GTAC 3 cut(s) 1458, 2082, 2200
AfiI CCNNNNNNNGG 9 cut(s) 779, 1331, 1499, 1639, 1962, 2024, 2099, 2165, 2383
AjnI CCWGG 4 cut(s) 354, 1482, 1492, 1568
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
Alw21I GWGCWC 2 cut(s) 1092, 2149
Alw26I GTCTC 3 cut(s) 238, 1057, 2047
AlwI GGATC 5 cut(s) 654, 1342, 1592, 1605, 2399
AlwNI CAGNNNCTG 1 cut(s) 1541
Ama87I CYCGRG 1 cut(s) 846
AoxI GGCC 3 cut(s) 596, 1074, 2110
ApeKI GCWGC 7 cut(s) 815, 880, 883, 1031, 1178, 1188, 1736
ApoI RAATTY 4 cut(s) 82, 823, 898, 2281
AseI ATTAAT 1 cut(s) 273
Asp700I GAANNNNTTC 5 cut(s) 751, 973, 1381, 1709, 1872
AspA2I CCTAGG 1 cut(s) 1466
AspLEI GCGC 3 cut(s) 129, 396, 2604
AspS9I GGNCC 3 cut(s) 596, 1572, 2171
AsuC2I CCSGG 2 cut(s) 2495, 2599
AsuHPI GGTGA 8 cut(s) 228, 444, 866, 1377, 1784, 1928, 2062, 2583
AvaI CYCGRG 1 cut(s) 846
AvaII GGWCC 2 cut(s) 1572, 2171
AvrII CCTAGG 1 cut(s) 1466
AxyI CCTNAGG 1 cut(s) 1762
BaeI ACNNNNGTAYC 2 cut(s) 1281, 1314
BamHI GGATCC 1 cut(s) 1597
BanI GGYRCC 1 cut(s) 393
BanII GRGCYC 3 cut(s) 75, 1092, 1256
BbsI GAAGAC 2 cut(s) 1284, 2147
Bbv12I GWGCWC 2 cut(s) 1092, 2149
BbvI GCAGC 7 cut(s) 827, 892, 895, 1018, 1190, 1200, 1723
BceAI ACGGC 1 cut(s) 1101
BciT130I CCWGG 4 cut(s) 356, 1484, 1494, 1570
BciVI GTATCC 2 cut(s) 1522, 2589
BcnI CCSGG 2 cut(s) 2495, 2599
BcoDI GTCTC 3 cut(s) 238, 1057, 2047
BfmI CTRYAG 3 cut(s) 1032, 1777, 2005
BfoI RGCGCY 1 cut(s) 397
BfuAI ACCTGC 1 cut(s) 2368
BfuI GTATCC 2 cut(s) 1522, 2589
BglII AGATCT 1 cut(s) 955
BisI GCNGC 7 cut(s) 816, 881, 884, 1032, 1179, 1189, 1737
BlnI CCTAGG 1 cut(s) 1466
BlpI GCTNAGC 1 cut(s) 1213
BlsI GCNGC 7 cut(s) 817, 882, 885, 1033, 1180, 1190, 1738
Bme1390I CCNGG 6 cut(s) 356, 1484, 1494, 1570, 2495, 2599
Bme18I GGWCC 2 cut(s) 1572, 2171
BmeT110I CYCGRG 1 cut(s) 846
BmgT120I GGNCC 3 cut(s) 596, 1572, 2171
BmiI GGNNCC 5 cut(s) 254, 395, 1168, 1599, 1644
BmrFI CCNGG 6 cut(s) 356, 1484, 1494, 1570, 2495, 2599
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 3 cut(s) 417, 537, 1226
BmuI ACTGGG 1 cut(s) 397
BpiI GAAGAC 2 cut(s) 1284, 2147
BpmI CTGGAG 1 cut(s) 338
Bpu10I CCTNAGC 1 cut(s) 19
Bpu1102I GCTNAGC 1 cut(s) 1213
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 2 cut(s) 2495, 2599
BsaBI GATNNNNATC 2 cut(s) 201, 2538
BsaHI GRCGYC 3 cut(s) 394, 1990, 2056
BsaI GGTCTC 1 cut(s) 1057
BsaJI CCNNGG 2 cut(s) 1068, 1466
BsaWI WCCGGW 1 cut(s) 1433
BsaXI ACNNNNNCTCC 2 cut(s) 2188, 2218
Bsc4I CCNNNNNNNGG 9 cut(s) 779, 1331, 1499, 1639, 1962, 2024, 2099, 2165, 2383
Bse118I RCCGGY 1 cut(s) 444
Bse1I ACTGG 6 cut(s) 392, 459, 594, 1599, 1831, 2274
Bse21I CCTNAGG 1 cut(s) 1762
Bse3DI GCAATG 3 cut(s) 39, 122, 1404
Bse8I GATNNNNATC 2 cut(s) 201, 2538
BseBI CCWGG 4 cut(s) 356, 1484, 1494, 1570
BseDI CCNNGG 2 cut(s) 1068, 1466
BseGI GGATG 5 cut(s) 64, 455, 552, 1256, 2392
BseJI GATNNNNATC 2 cut(s) 201, 2538
BseLI CCNNNNNNNGG 9 cut(s) 779, 1331, 1499, 1639, 1962, 2024, 2099, 2165, 2383
BseMI GCAATG 3 cut(s) 39, 122, 1404
BseMII CTCAG 7 cut(s) 759, 1227, 1574, 1683, 1776, 2436, 2499
BseNI ACTGG 6 cut(s) 392, 459, 594, 1599, 1831, 2274
BseRI GAGGAG 3 cut(s) 1600, 1978, 2574
BseXI GCAGC 7 cut(s) 827, 892, 895, 1018, 1190, 1200, 1723
BsgI GTGCAG 1 cut(s) 1197
Bsh1236I CGCG 1 cut(s) 2604
BshFI GGCC 3 cut(s) 598, 1076, 2112
BshNI GGYRCC 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 1092, 2149
BsiHKCI CYCGRG 1 cut(s) 846
BsiSI CCGG 4 cut(s) 445, 1434, 2494, 2598
BslFI GGGAC 2 cut(s) 1088, 2359
BslI CCNNNNNNNGG 9 cut(s) 779, 1331, 1499, 1639, 1962, 2024, 2099, 2165, 2383
BsmAI GTCTC 3 cut(s) 238, 1057, 2047
BsmBI CGTCTC 1 cut(s) 2047
BsmFI GGGAC 2 cut(s) 1088, 2359
BsmI GAATGC 1 cut(s) 2435
BsnI GGCC 3 cut(s) 598, 1076, 2112
Bso31I GGTCTC 1 cut(s) 1057
BsoBI CYCGRG 1 cut(s) 846
Bsp1286I GDGCHC 4 cut(s) 75, 1092, 1256, 2149
Bsp1407I TGTACA 2 cut(s) 1456, 2080
Bsp1720I GCTNAGC 1 cut(s) 1213
BspACI CCGC 7 cut(s) 790, 927, 1393, 1640, 1698, 1807, 2021
BspANI GGCC 3 cut(s) 598, 1076, 2112
BspCNI CTCAG 7 cut(s) 760, 1226, 1575, 1684, 1775, 2435, 2498
BspFNI CGCG 1 cut(s) 2604
BspHI TCATGA 2 cut(s) 576, 1864
BspLI GGNNCC 5 cut(s) 254, 395, 1168, 1599, 1644
BspMAI CTGCAG 2 cut(s) 1036, 1781
BspMI ACCTGC 1 cut(s) 2368
BspPI GGATC 5 cut(s) 654, 1342, 1592, 1605, 2399
BspQI GCTCTTC 1 cut(s) 1387
BspT107I GGYRCC 1 cut(s) 393
BspTNI GGTCTC 1 cut(s) 1057
BsrDI GCAATG 3 cut(s) 39, 122, 1404
BsrFI RCCGGY 1 cut(s) 444
BsrGI TGTACA 2 cut(s) 1456, 2080
BsrI ACTGG 6 cut(s) 392, 459, 594, 1599, 1831, 2274
BssAI RCCGGY 1 cut(s) 444
BssECI CCNNGG 2 cut(s) 1068, 1466
BssNI GRCGYC 3 cut(s) 394, 1990, 2056
BssT1I CCWWGG 2 cut(s) 1068, 1466
Bst2UI CCWGG 4 cut(s) 356, 1484, 1494, 1570
Bst4CI ACNGT 9 cut(s) 554, 738, 1224, 1315, 1914, 1943, 2128, 2219, 2512
Bst6I CTCTTC 1 cut(s) 1387
BstACI GRCGYC 3 cut(s) 394, 1990, 2056
BstAPI GCANNNNNTGC 1 cut(s) 2570
BstAUI TGTACA 2 cut(s) 1456, 2080
BstC8I GCNNGC 5 cut(s) 800, 1055, 1805, 2019, 2090
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 5 cut(s) 64, 455, 552, 1256, 2392
BstFNI CGCG 1 cut(s) 2604
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 3 cut(s) 129, 396, 2604
BstMAI GTCTC 3 cut(s) 238, 1057, 2047
BstMWI GCNNNNNNNGC 5 cut(s) 70, 1082, 1401, 2118, 2570
BstNI CCWGG 4 cut(s) 356, 1484, 1494, 1570
BstSCI CCNGG 6 cut(s) 354, 1482, 1492, 1568, 2493, 2597
BstSFI CTRYAG 3 cut(s) 1032, 1777, 2005
BstUI CGCG 1 cut(s) 2604
BstV1I GCAGC 7 cut(s) 827, 892, 895, 1018, 1190, 1200, 1723
BstV2I GAAGAC 2 cut(s) 1284, 2147
BstX2I RGATCY 4 cut(s) 646, 955, 1597, 2391
BstXI CCANNNNNNTGG 1 cut(s) 1525
BstYI RGATCY 4 cut(s) 646, 955, 1597, 2391
Bsu36I CCTNAGG 1 cut(s) 1762
BsuI GTATCC 2 cut(s) 1522, 2589
BsuRI GGCC 3 cut(s) 598, 1076, 2112
BtsCI GGATG 5 cut(s) 64, 455, 552, 1256, 2392
BtsI GCAGTG 3 cut(s) 1013, 1601, 2460
BveI ACCTGC 1 cut(s) 2368
Cac8I GCNNGC 5 cut(s) 800, 1055, 1805, 2019, 2090
CaiI CAGNNNCTG 1 cut(s) 1541
CciI TCATGA 2 cut(s) 576, 1864
CfoI GCGC 3 cut(s) 129, 396, 2604
Cfr10I RCCGGY 1 cut(s) 444
Cfr13I GGNCC 3 cut(s) 596, 1572, 2171
CseI GACGC 3 cut(s) 1979, 2064, 2593
CsiI ACCWGGT 1 cut(s) 1492
Csp6I GTAC 3 cut(s) 1457, 2081, 2199
CviAII CATG 8 cut(s) 55, 206, 241, 477, 577, 1844, 1865, 2211
CviQI GTAC 3 cut(s) 1457, 2081, 2199
DinI GGCGCC 1 cut(s) 395
DraI TTTAAA 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 1387
EarI CTCTTC 1 cut(s) 1387
EciI GGCGGA 1 cut(s) 1382
Ecl136II GAGCTC 1 cut(s) 1090
Eco130I CCWWGG 2 cut(s) 1068, 1466
Eco147I AGGCCT 2 cut(s) 1076, 2112
Eco24I GRGCYC 3 cut(s) 75, 1092, 1256
Eco31I GGTCTC 1 cut(s) 1057
Eco47I GGWCC 2 cut(s) 1572, 2171
Eco53kI GAGCTC 1 cut(s) 1090
Eco57I CTGAAG 9 cut(s) 309, 624, 939, 1132, 1149, 1434, 1518, 1628, 1783
Eco81I CCTNAGG 1 cut(s) 1762
Eco88I CYCGRG 1 cut(s) 846
EcoICRI GAGCTC 1 cut(s) 1090
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoO109I RGGNCCY 1 cut(s) 596
EcoRII CCWGG 4 cut(s) 354, 1482, 1492, 1568
EcoT14I CCWWGG 2 cut(s) 1068, 1466
EcoT38I GRGCYC 3 cut(s) 75, 1092, 1256
EgeI GGCGCC 1 cut(s) 395
EheI GGCGCC 1 cut(s) 395
ErhI CCWWGG 2 cut(s) 1068, 1466
Esp3I CGTCTC 1 cut(s) 2047
FaeI CATG 8 cut(s) 58, 209, 244, 480, 580, 1847, 1868, 2214
FaqI GGGAC 2 cut(s) 1088, 2359
FatI CATG 8 cut(s) 54, 205, 240, 476, 576, 1843, 1864, 2210
FauI CCCGC 2 cut(s) 1691, 1800
Fnu4HI GCNGC 7 cut(s) 816, 881, 884, 1032, 1179, 1189, 1737
FokI GGATG 5 cut(s) 71, 442, 559, 1243, 2399
FriOI GRGCYC 3 cut(s) 75, 1092, 1256
Fsp4HI GCNGC 7 cut(s) 816, 881, 884, 1032, 1179, 1189, 1737
GlaI GCGC 3 cut(s) 128, 395, 2603
GluI GCNGC 7 cut(s) 816, 881, 884, 1032, 1179, 1189, 1737
GsuI CTGGAG 1 cut(s) 338
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 3 cut(s) 598, 1076, 2112
HapII CCGG 4 cut(s) 445, 1434, 2494, 2598
HgaI GACGC 3 cut(s) 1979, 2064, 2593
HhaI GCGC 3 cut(s) 129, 396, 2604
Hin1I GRCGYC 3 cut(s) 394, 1990, 2056
Hin1II CATG 8 cut(s) 58, 209, 244, 480, 580, 1847, 1868, 2214
Hin6I GCGC 3 cut(s) 127, 394, 2602
HinP1I GCGC 3 cut(s) 127, 394, 2602
HincII GTYRAC 1 cut(s) 1473
HindII GTYRAC 1 cut(s) 1473
HindIII AAGCTT 2 cut(s) 363, 1051
HpaII CCGG 4 cut(s) 445, 1434, 2494, 2598
HphI GGTGA 8 cut(s) 228, 444, 866, 1377, 1784, 1928, 2062, 2583
Hpy166II GTNNAC 2 cut(s) 1142, 1473
Hpy8I GTNNAC 2 cut(s) 1142, 1473
Hpy99I CGWCG 2 cut(s) 1050, 1995
HpyCH4III ACNGT 9 cut(s) 554, 738, 1224, 1315, 1914, 1943, 2128, 2219, 2512
HpyCH4IV ACGT 4 cut(s) 268, 729, 1509, 2191
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 1082, 1401, 2118, 2570
HpySE526I ACGT 4 cut(s) 268, 729, 1509, 2191
Hsp92I GRCGYC 3 cut(s) 394, 1990, 2056
Hsp92II CATG 8 cut(s) 58, 209, 244, 480, 580, 1847, 1868, 2214
HspAI GCGC 3 cut(s) 127, 394, 2602
KasI GGCGCC 1 cut(s) 393
LguI GCTCTTC 1 cut(s) 1387
LmnI GCTCC 2 cut(s) 1172, 1642
Lsp1109I GCAGC 7 cut(s) 827, 892, 895, 1018, 1190, 1200, 1723
LweI GCATC 3 cut(s) 417, 537, 1226
MabI ACCWGGT 1 cut(s) 1492
MaeII ACGT 4 cut(s) 268, 729, 1509, 2191
MaeIII GTNAC 7 cut(s) 518, 665, 1357, 1590, 1772, 2009, 2213
MflI RGATCY 4 cut(s) 646, 955, 1597, 2391
MhlI GDGCHC 4 cut(s) 75, 1092, 1256, 2149
Mly113I GGCGCC 1 cut(s) 394
MlyI GAGTC 2 cut(s) 690, 1975
MmeI TCCRAC 3 cut(s) 355, 613, 2174
MroXI GAANNNNTTC 5 cut(s) 751, 973, 1381, 1709, 1872
MseI TTAA 9 cut(s) 225, 273, 368, 651, 827, 1343, 1400, 2366, 2409
MslI CAYNNNNRTG 2 cut(s) 728, 1409
MspA1I CMGCKG 2 cut(s) 525, 1640
MspI CCGG 4 cut(s) 445, 1434, 2494, 2598
MspR9I CCNGG 6 cut(s) 356, 1484, 1494, 1570, 2495, 2599
Mva1269I GAATGC 1 cut(s) 2435
MvaI CCWGG 4 cut(s) 356, 1484, 1494, 1570
MvnI CGCG 1 cut(s) 2604
MwoI GCNNNNNNNGC 5 cut(s) 70, 1082, 1401, 2118, 2570
NarI GGCGCC 1 cut(s) 394
NciI CCSGG 2 cut(s) 2495, 2599
NlaIII CATG 8 cut(s) 58, 209, 244, 480, 580, 1847, 1868, 2214
NlaIV GGNNCC 5 cut(s) 254, 395, 1168, 1599, 1644
NmeAIII GCCGAG 1 cut(s) 1143
NmuCI GTSAC 4 cut(s) 518, 1772, 2009, 2213
PaeR7I CTCGAG 1 cut(s) 846
PagI TCATGA 2 cut(s) 576, 1864
PceI AGGCCT 2 cut(s) 1076, 2112
PciSI GCTCTTC 1 cut(s) 1387
PctI GAATGC 1 cut(s) 2435
PdmI GAANNNNTTC 5 cut(s) 751, 973, 1381, 1709, 1872
PfoI TCCNGGA 2 cut(s) 354, 2493
PkrI GCNGC 7 cut(s) 817, 882, 885, 1033, 1180, 1190, 1738
PleI GAGTC 2 cut(s) 689, 1974
PluTI GGCGCC 1 cut(s) 397
PpsI GAGTC 2 cut(s) 689, 1974
PshBI ATTAAT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 1092
Psp1406I AACGTT 1 cut(s) 2191
Psp6I CCWGG 4 cut(s) 354, 1482, 1492, 1568
PspGI CCWGG 4 cut(s) 354, 1482, 1492, 1568
PspN4I GGNNCC 5 cut(s) 254, 395, 1168, 1599, 1644
PspPI GGNCC 3 cut(s) 596, 1572, 2171
PstI CTGCAG 2 cut(s) 1036, 1781
PstNI CAGNNNCTG 1 cut(s) 1541
PsuI RGATCY 4 cut(s) 646, 955, 1597, 2391
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 3 cut(s) 1458, 2082, 2200
RsaNI GTAC 3 cut(s) 1457, 2081, 2199
RseI CAYNNNNRTG 2 cut(s) 728, 1409
SacI GAGCTC 1 cut(s) 1092
SapI GCTCTTC 1 cut(s) 1387
SaqAI TTAA 9 cut(s) 225, 273, 368, 651, 827, 1343, 1400, 2366, 2409
SatI GCNGC 7 cut(s) 816, 881, 884, 1032, 1179, 1189, 1737
Sau96I GGNCC 3 cut(s) 596, 1572, 2171
SchI GAGTC 2 cut(s) 690, 1975
ScrFI CCNGG 6 cut(s) 356, 1484, 1494, 1570, 2495, 2599
SduI GDGCHC 4 cut(s) 75, 1092, 1256, 2149
SexAI ACCWGGT 1 cut(s) 1492
SfaNI GCATC 3 cut(s) 417, 537, 1226
SfcI CTRYAG 3 cut(s) 1032, 1777, 2005
SfoI GGCGCC 1 cut(s) 395
Sfr274I CTCGAG 1 cut(s) 846
SinI GGWCC 2 cut(s) 1572, 2171
SlaI CTCGAG 1 cut(s) 846
SmiMI CAYNNNNRTG 2 cut(s) 728, 1409
SmlI CTYRAG 2 cut(s) 335, 846
SmoI CTYRAG 2 cut(s) 335, 846
SseBI AGGCCT 2 cut(s) 1076, 2112
SsiI CCGC 7 cut(s) 790, 927, 1393, 1640, 1698, 1807, 2021
SspDI GGCGCC 1 cut(s) 393
SstI GAGCTC 1 cut(s) 1092
StuI AGGCCT 2 cut(s) 1076, 2112
StyD4I CCNGG 6 cut(s) 354, 1482, 1492, 1568, 2493, 2597
StyI CCWWGG 2 cut(s) 1068, 1466
TaaI ACNGT 9 cut(s) 554, 738, 1224, 1315, 1914, 1943, 2128, 2219, 2512
TaiI ACGT 4 cut(s) 271, 732, 1512, 2194
TaqI TCGA 3 cut(s) 847, 1045, 1932
TatI WGTACW 3 cut(s) 1456, 2080, 2198
Tru1I TTAA 9 cut(s) 225, 273, 368, 651, 827, 1343, 1400, 2366, 2409
Tru9I TTAA 9 cut(s) 225, 273, 368, 651, 827, 1343, 1400, 2366, 2409
TseFI GTSAC 4 cut(s) 518, 1772, 2009, 2213
TseI GCWGC 7 cut(s) 815, 880, 883, 1031, 1178, 1188, 1736
Tsp45I GTSAC 4 cut(s) 518, 1772, 2009, 2213
TspDTI ATGAA 6 cut(s) 312, 507, 565, 1760, 1881, 1910
TspGWI ACGGA 1 cut(s) 2263
VpaK11BI GGWCC 2 cut(s) 1572, 2171
VspI ATTAAT 1 cut(s) 273
XagI CCTNNNNNAGG 1 cut(s) 1329
XapI RAATTY 4 cut(s) 82, 823, 898, 2281
XbaI TCTAGA 1 cut(s) 1979
XcmI CCANNNNNNNNNTGG 1 cut(s) 1490
XhoI CTCGAG 1 cut(s) 846
XmaJI CCTAGG 1 cut(s) 1466
XmnI GAANNNNTTC 5 cut(s) 751, 973, 1381, 1709, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.