Rorug05G0382800

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007762
Physical Location & Seq
Forward (+)
51916619 .. 51918098
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0382800.1

Sequence Viewer

Length: 522 bp
ATGCTGATTGAGCATGAACAGTTACATGTGGACAAAAACAATGGGGTTGAAGTTGGCCGGACAATAAAGAAGTTTCCACTGTTGACACCTAGAGAATATGTATTAGCTTGGAGATTGTGGGAAGGAAAAGATAAAACATTCTACTATTTTGTCAAGGAATGTGAACATTCTTTGGCACCATGGCAGAAGAAGTATGTGCGTGTTGGTACTTTCAGATCTGGTTGGCGAATTAGAAAAGTACCTGGTAGAAATGCCTGCGAGATCAAAATGTATCACCAAGAAGATGCTGGTTTAAACGTGGAGATGGCAAAACTGGTCTTCGTGAAGGGCATATGGAGTTACGTATGTAAGATGGATAGTGCACTACGCAAATATTCTGCAATCAGCAATGATCAATCAAGTTCTGCTACCACTGCAGTCACTTTGATAAAAAAGGTCCCCCCTGGATTAGAGGCCACGGATAGCAATGGAGCAACCTCTGCAGAACCCAATGTTCAGAGTCTTAAATTGTTGTTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

173

Amino Acids

19.75

Weight (kDa)

9.4

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 175
AcoI YGGCCR 1 cut(s) 55
AfaI GTAC 2 cut(s) 208, 240
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 1 cut(s) 50
AjnI CCWGG 2 cut(s) 241, 442
AluBI AGCT 1 cut(s) 107
AluI AGCT 1 cut(s) 107
Alw21I GWGCWC 1 cut(s) 364
Alw44I GTGCAC 1 cut(s) 360
AoxI GGCC 2 cut(s) 55, 453
ApaLI GTGCAC 1 cut(s) 360
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 266
AvaII GGWCC 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 364
BccI CCATC 2 cut(s) 298, 346
BciT130I CCWGG 2 cut(s) 243, 444
BclI TGATCA 1 cut(s) 391
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 2 cut(s) 414, 480
BglII AGATCT 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 243, 444
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 2 cut(s) 177, 438
BmrFI CCNGG 2 cut(s) 243, 444
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 343
BsaJI CCNNGG 3 cut(s) 179, 442, 456
Bse1I ACTGG 1 cut(s) 318
Bse3DI GCAATG 2 cut(s) 394, 472
BseBI CCWGG 2 cut(s) 243, 444
BseDI CCNNGG 3 cut(s) 179, 442, 456
BseMI GCAATG 2 cut(s) 394, 472
BseNI ACTGG 1 cut(s) 318
BseSI GKGCMC 1 cut(s) 364
BshFI GGCC 2 cut(s) 57, 455
BshNI GGYRCC 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 422
BsnI GGCC 2 cut(s) 57, 455
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 3 cut(s) 215, 261, 391
Bsp19I CCATGG 1 cut(s) 179
BspANI GGCC 2 cut(s) 57, 455
BspLI GGNNCC 2 cut(s) 177, 438
BspMAI CTGCAG 2 cut(s) 418, 484
BspT107I GGYRCC 1 cut(s) 175
BsrDI GCAATG 2 cut(s) 394, 472
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 3 cut(s) 179, 442, 456
BssMI GATC 3 cut(s) 215, 261, 391
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 2 cut(s) 243, 444
Bst4CI ACNGT 2 cut(s) 21, 81
BstAPI GCANNNNNTGC 1 cut(s) 479
BstBAI YACGTR 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 256
BstDSI CCRYGG 2 cut(s) 179, 456
BstKTI GATC 3 cut(s) 218, 264, 394
BstMBI GATC 3 cut(s) 215, 261, 391
BstMWI GCNNNNNNNGC 3 cut(s) 10, 413, 479
BstNI CCWGG 2 cut(s) 243, 444
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 241, 442
BstSFI CTRYAG 2 cut(s) 414, 480
BstSLI GKGCMC 1 cut(s) 364
BstSNI TACGTA 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 2 cut(s) 57, 455
BtgI CCRYGG 2 cut(s) 179, 456
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 77, 411
Cac8I GCNNGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 436
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 2 cut(s) 207, 239
CviAII CATG 3 cut(s) 14, 26, 180
CviJI RGCY 3 cut(s) 57, 107, 455
CviKI_1 RGCY 3 cut(s) 57, 107, 455
CviQI GTAC 2 cut(s) 207, 239
DpnI GATC 3 cut(s) 217, 263, 393
DpnII GATC 3 cut(s) 215, 261, 391
DraI TTTAAA 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 55
Eco105I TACGTA 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 436
EcoO109I RGGNCCY 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 241, 442
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 3 cut(s) 17, 29, 183
FaiI YATR 8 cut(s) 15, 27, 99, 181, 195, 332, 334, 346
FaqI GGGAC 1 cut(s) 422
FatI CATG 3 cut(s) 13, 25, 179
FauNDI CATATG 1 cut(s) 332
FbaI TGATCA 1 cut(s) 391
FspBI CTAG 1 cut(s) 90
HaeIII GGCC 2 cut(s) 57, 455
HapII CCGG 1 cut(s) 58
Hin1II CATG 3 cut(s) 17, 29, 183
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 1 cut(s) 499
HpaII CCGG 1 cut(s) 58
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 4 cut(s) 31, 84, 164, 362
Hpy188I TCNGA 2 cut(s) 215, 498
Hpy188III TCNNGA 1 cut(s) 322
Hpy8I GTNNAC 4 cut(s) 31, 84, 164, 362
HpyAV CCTTC 2 cut(s) 116, 319
HpyCH4III ACNGT 2 cut(s) 21, 81
HpyCH4IV ACGT 2 cut(s) 297, 342
HpyCH4V TGCA 5 cut(s) 362, 380, 416, 482, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 413, 479
HpySE526I ACGT 2 cut(s) 297, 342
Hsp92II CATG 3 cut(s) 17, 29, 183
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 215, 261, 391
LmnI GCTCC 1 cut(s) 470
LpnPI CCDG 9 cut(s) 71, 204, 228, 255, 268, 273, 299, 429, 456
LweI GCATC 1 cut(s) 274
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 1 cut(s) 90
MaeII ACGT 2 cut(s) 297, 342
MaeIII GTNAC 3 cut(s) 21, 338, 418
MalI GATC 3 cut(s) 217, 263, 393
MboI GATC 3 cut(s) 215, 261, 391
MboII GAAGA 3 cut(s) 199, 293, 310
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 2 cut(s) 228, 506
MlyI GAGTC 1 cut(s) 508
MnlI CCTC 2 cut(s) 445, 487
MseI TTAA 2 cut(s) 293, 504
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 243, 444
MssI GTTTAAAC 1 cut(s) 294
MvaI CCWGG 2 cut(s) 243, 444
MwoI GCNNNNNNNGC 3 cut(s) 10, 413, 479
NcoI CCATGG 1 cut(s) 179
NdeI CATATG 1 cut(s) 332
NdeII GATC 3 cut(s) 215, 261, 391
NlaIII CATG 3 cut(s) 17, 29, 183
NlaIV GGNNCC 2 cut(s) 177, 438
NmuCI GTSAC 1 cut(s) 418
NspI RCATGY 1 cut(s) 29
PciI ACATGT 1 cut(s) 25
PleI GAGTC 1 cut(s) 507
PmeI GTTTAAAC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 507
Ppu21I YACGTR 1 cut(s) 343
PpuMI RGGWCCY 1 cut(s) 436
PscI ACATGT 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 436
Psp6I CCWGG 2 cut(s) 241, 442
PspGI CCWGG 2 cut(s) 241, 442
PspN4I GGNNCC 2 cut(s) 177, 438
PspPI GGNCC 1 cut(s) 436
PspPPI RGGWCCY 1 cut(s) 436
PstI CTGCAG 2 cut(s) 418, 484
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 2 cut(s) 208, 240
RsaNI GTAC 2 cut(s) 207, 239
SaqAI TTAA 2 cut(s) 293, 504
Sau3AI GATC 3 cut(s) 215, 261, 391
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 2 cut(s) 243, 444
SduI GDGCHC 1 cut(s) 364
SetI ASST 7 cut(s) 91, 109, 244, 300, 345, 438, 479
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 2 cut(s) 414, 480
SinI GGWCC 1 cut(s) 436
SnaBI TACGTA 1 cut(s) 343
Sse9I AATT 2 cut(s) 228, 506
SspI AATATT 1 cut(s) 374
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 241, 442
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 2 cut(s) 21, 81
TaiI ACGT 2 cut(s) 300, 345
TasI AATT 2 cut(s) 228, 506
Tru1I TTAA 2 cut(s) 293, 504
Tru9I TTAA 2 cut(s) 293, 504
TscAI CASTG 2 cut(s) 84, 418
TseFI GTSAC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 30
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 2 cut(s) 84, 418
VneI GTGCAC 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 436
XceI RCATGY 1 cut(s) 29
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.