Rorug05G0382900

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007762
Physical Location & Seq
Reverse (-)
51935338 .. 51935574
237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0382900.1

Sequence Viewer

Length: 237 bp
ATGGACAAGGTTAAGTTTCATGACTTTGTAAATCAGATGCTAGCGCAGGGCGCCAAGGTGAGGAACACTGTTGTGGATGCCAAGACTCACGCCATCACTCTCGTGGCGGAGGATAGGGTTTTTCCTACGCCCTTACATACACACTTGCCATCTAAGGAGGTTGCCATAGTCCGTCCAACAGAGCCGGCCCCGTCTGAGGGTGAAGACGCTGAGGCATCATGCTTCGGCCAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

78

Amino Acids

8.54

Weight (kDa)

5.7

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 50
AciI CCGC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 51
AfiI CCNNNNNNNGG 3 cut(s) 60, 196, 197
AleI CACNNNNGTG 2 cut(s) 71, 101
AoxI GGCC 2 cut(s) 186, 226
AspLEI GCGC 2 cut(s) 46, 53
AspS9I GGNCC 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 70, 212
AsuNHI GCTAGC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 50
BauI CACGAG 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 210
BccI CCATC 2 cut(s) 101, 157
BfaI CTAG 2 cut(s) 41, 235
BfoI RGCGCY 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 2 cut(s) 52, 189
BmsI GCATC 3 cut(s) 27, 67, 224
BmtI GCTAGC 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 196, 197
Bse118I RCCGGY 1 cut(s) 184
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 3 cut(s) 60, 196, 197
BseMII CTCAG 2 cut(s) 186, 201
BshFI GGCC 2 cut(s) 188, 228
BshNI GGYRCC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 3 cut(s) 60, 196, 197
BsnI GGCC 2 cut(s) 188, 228
BspACI CCGC 1 cut(s) 107
BspANI GGCC 2 cut(s) 188, 228
BspCNI CTCAG 2 cut(s) 187, 202
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 52, 189
BspOI GCTAGC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 54
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 54
Bst2BI CACGAG 1 cut(s) 101
Bst4CI ACNGT 1 cut(s) 70
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 42, 186
BstDEI CTNAG 3 cut(s) 153, 195, 210
BstF5I GGATG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 46, 53
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstV2I GAAGAC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 188, 228
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 42, 186
CciI TCATGA 1 cut(s) 19
CfoI GCGC 2 cut(s) 46, 53
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 187
CseI GACGC 1 cut(s) 215
CviAII CATG 2 cut(s) 20, 219
CviJI RGCY 3 cut(s) 184, 188, 228
CviKI_1 RGCY 3 cut(s) 184, 188, 228
DdeI CTNAG 3 cut(s) 153, 195, 210
DinI GGCGCC 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 226
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 2 cut(s) 23, 222
FaiI YATR 4 cut(s) 21, 138, 167, 220
FatI CATG 2 cut(s) 19, 218
FokI GGATG 1 cut(s) 89
FspBI CTAG 2 cut(s) 41, 235
GlaI GCGC 2 cut(s) 45, 52
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 2 cut(s) 188, 228
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 46, 53
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 2 cut(s) 23, 222
Hin6I GCGC 2 cut(s) 44, 51
HinP1I GCGC 2 cut(s) 44, 51
HinfI GANTC 1 cut(s) 85
HpaII CCGG 1 cut(s) 185
HphI GGTGA 2 cut(s) 70, 212
Hpy188I TCNGA 2 cut(s) 36, 196
Hpy188III TCNNGA 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 3 cut(s) 153, 195, 210
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 2 cut(s) 23, 222
HspAI GCGC 2 cut(s) 44, 51
KasI GGCGCC 1 cut(s) 50
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
LpnPI CCDG 2 cut(s) 32, 198
LweI GCATC 3 cut(s) 27, 67, 224
MaeI CTAG 2 cut(s) 41, 235
MboII GAAGA 1 cut(s) 215
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 200
MnlI CCTC 5 cut(s) 54, 103, 151, 190, 205
MroNI GCCGGC 1 cut(s) 184
MseI TTAA 1 cut(s) 12
MslI CAYNNNNRTG 2 cut(s) 71, 101
MspI CCGG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 186
NarI GGCGCC 1 cut(s) 51
NgoMIV GCCGGC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 2 cut(s) 23, 222
NlaIV GGNNCC 2 cut(s) 52, 189
OliI CACNNNNGTG 2 cut(s) 71, 101
PagI TCATGA 1 cut(s) 19
PdiI GCCGGC 1 cut(s) 186
PleI GAGTC 1 cut(s) 79
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 52, 189
PspPI GGNCC 1 cut(s) 187
RseI CAYNNNNRTG 2 cut(s) 71, 101
SaqAI TTAA 1 cut(s) 12
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 1 cut(s) 79
SetI ASST 3 cut(s) 12, 60, 162
SfaNI GCATC 3 cut(s) 27, 67, 224
SfoI GGCGCC 1 cut(s) 52
SmiMI CAYNNNNRTG 2 cut(s) 71, 101
SsiI CCGC 1 cut(s) 107
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 2 cut(s) 41, 235
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 70
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 1 cut(s) 73
TspDTI ATGAA 1 cut(s) 8
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XspI CTAG 2 cut(s) 41, 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.