Rroxscaffold_1G00022910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017817
Physical Location & Seq
Reverse (-)
28340135 .. 28358954
18820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022910.1

Sequence Viewer

Length: 462 bp
ATGCAACACGTGGTGAAAGGAAGAAGTGAAGCTTCTAGTTGCCCTCCCGCACCGAACATCCGGAATTCTTGGGATAACTCAAATGAAGACGGACACAACGGTCTATCCAAGAAATCCAAGGCTCCTAGGAAGGTTGTACCGGCTAAAAAGGAAACTTCCGACCAACACTTAGCCTCCGCCTTTAGTCCGAACCCTTCGCCGACATCGCTTCTTCGATACATGGTTCTTTGCTTGCTTGCCATGAATATGGATATCGTAAGAGATGAAGGAAAGTTTGCTAGTGTTAAACCAAAGAATTGTGGGCTATCCGTGGTTGCTGCATCTGCAGCTCAATCAGCTGCAACTATAGTACAGGTTGGCACAAAAAGCTTACCTCTAAGGGTGTTTGTAGTCCATGGATCAGATGAGTCCACTCTGGTTGAAGATGCATTTGAGTTTGGCAAGATCATACTGGACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.41

Weight (kDa)

8.83

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 2 cut(s) 48, 177
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 1 cut(s) 64
AcvI CACGTG 1 cut(s) 10
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 2 cut(s) 138, 351
AflIII ACRYGT 1 cut(s) 7
AgsI TTSAA 1 cut(s) 422
AluBI AGCT 4 cut(s) 32, 329, 338, 369
AluI AGCT 4 cut(s) 32, 329, 338, 369
AlwI GGATC 1 cut(s) 406
Aor13HI TCCGGA 1 cut(s) 60
ApeKI GCWGC 3 cut(s) 317, 326, 338
ApoI RAATTY 1 cut(s) 64
AspA2I CCTAGG 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 454
AvrII CCTAGG 1 cut(s) 125
BbrPI CACGTG 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 93
BbvI GCAGC 3 cut(s) 304, 325, 338
BfaI CTAG 3 cut(s) 36, 126, 279
BfmI CTRYAG 2 cut(s) 324, 345
BisI GCNGC 3 cut(s) 318, 327, 339
BlnI CCTAGG 1 cut(s) 125
BlsI GCNGC 3 cut(s) 319, 328, 340
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 123
BmsI GCATC 2 cut(s) 329, 415
BpiI GAAGAC 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 10
BsaJI CCNNGG 4 cut(s) 117, 125, 309, 394
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 60
BseDI CCNNGG 4 cut(s) 117, 125, 309, 394
BseGI GGATG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 456
BseXI GCAGC 3 cut(s) 304, 325, 338
BsiSI CCGG 2 cut(s) 61, 140
Bsp13I TCCGGA 1 cut(s) 60
Bsp143I GATC 2 cut(s) 398, 444
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 2 cut(s) 48, 177
BspEI TCCGGA 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 1 cut(s) 456
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 4 cut(s) 117, 125, 309, 394
BssMI GATC 2 cut(s) 398, 444
BssT1I CCWWGG 3 cut(s) 117, 125, 394
Bst4CI ACNGT 1 cut(s) 101
BstBAI YACGTR 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 233, 237
BstDEI CTNAG 2 cut(s) 169, 377
BstDSI CCRYGG 2 cut(s) 309, 394
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 2 cut(s) 401, 447
BstMBI GATC 2 cut(s) 398, 444
BstMWI GCNNNNNNNGC 5 cut(s) 205, 323, 326, 335, 366
BstSFI CTRYAG 2 cut(s) 324, 345
BstV1I GCAGC 3 cut(s) 304, 325, 338
BstV2I GAAGAC 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 247
BtgI CCRYGG 2 cut(s) 309, 394
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 233, 237
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 2 cut(s) 137, 350
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 3 cut(s) 220, 241, 395
CviJI RGCY 8 cut(s) 32, 122, 143, 173, 304, 329, 338, 369
CviKI_1 RGCY 8 cut(s) 32, 122, 143, 173, 304, 329, 338, 369
CviQI GTAC 2 cut(s) 137, 350
DdeI CTNAG 2 cut(s) 169, 377
DpnI GATC 2 cut(s) 400, 446
DpnII GATC 2 cut(s) 398, 444
DraIII CACNNNGTG 1 cut(s) 13
DrdI GACNNNNNNGTC 1 cut(s) 99
DseDI GACNNNNNNGTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 117, 125, 394
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 454
Eco72I CACGTG 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 64
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 3 cut(s) 117, 125, 394
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 3 cut(s) 117, 125, 394
FaeI CATG 3 cut(s) 223, 244, 398
FaiI YATR 6 cut(s) 221, 242, 248, 347, 396, 449
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FatI CATG 3 cut(s) 219, 240, 394
FauI CCCGC 1 cut(s) 55
Fnu4HI GCNGC 3 cut(s) 318, 327, 339
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 318, 327, 339
FspBI CTAG 3 cut(s) 36, 126, 279
GluI GCNGC 3 cut(s) 318, 327, 339
HapII CCGG 2 cut(s) 61, 140
Hin1II CATG 3 cut(s) 223, 244, 398
HindIII AAGCTT 2 cut(s) 30, 367
HinfI GANTC 1 cut(s) 407
HpaII CCGG 2 cut(s) 61, 140
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 3 cut(s) 160, 189, 403
Hpy188III TCNNGA 1 cut(s) 61
Hpy8I GTNNAC 1 cut(s) 411
HpyAV CCTTC 3 cut(s) 124, 204, 260
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 5 cut(s) 4, 320, 326, 341, 428
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 323, 326, 335, 366
HpyF3I CTNAG 2 cut(s) 169, 377
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 3 cut(s) 223, 244, 398
Kpn2I TCCGGA 1 cut(s) 60
Kzo9I GATC 2 cut(s) 398, 444
LmnI GCTCC 1 cut(s) 127
LpnPI CCDG 5 cut(s) 74, 153, 338, 401, 437
Lsp1109I GCAGC 3 cut(s) 304, 325, 338
LweI GCATC 2 cut(s) 329, 415
MaeI CTAG 3 cut(s) 36, 126, 279
MaeII ACGT 1 cut(s) 9
MalI GATC 2 cut(s) 400, 446
MboI GATC 2 cut(s) 398, 444
MboII GAAGA 4 cut(s) 33, 98, 203, 434
MluCI AATT 2 cut(s) 64, 295
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 3 cut(s) 54, 184, 384
Mph1103I ATGCAT 1 cut(s) 430
MroI TCCGGA 1 cut(s) 60
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 2 cut(s) 61, 140
MwoI GCNNNNNNNGC 5 cut(s) 205, 323, 326, 335, 366
NcoI CCATGG 1 cut(s) 394
NdeII GATC 2 cut(s) 398, 444
NlaIII CATG 3 cut(s) 223, 244, 398
NlaIV GGNNCC 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 430
PkrI GCNGC 3 cut(s) 319, 328, 340
PleI GAGTC 1 cut(s) 415
PmaCI CACGTG 1 cut(s) 10
PmlI CACGTG 1 cut(s) 10
PpsI GAGTC 1 cut(s) 415
Ppu21I YACGTR 1 cut(s) 10
PspCI CACGTG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 138, 351
RsaNI GTAC 2 cut(s) 137, 350
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 318, 327, 339
Sau3AI GATC 2 cut(s) 398, 444
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 1 cut(s) 416
SetI ASST 8 cut(s) 12, 34, 135, 331, 340, 357, 371, 376
SfaNI GCATC 2 cut(s) 329, 415
SfcI CTRYAG 2 cut(s) 324, 345
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 245
Sse9I AATT 2 cut(s) 64, 295
SsiI CCGC 2 cut(s) 48, 177
SspMI CTAG 3 cut(s) 36, 126, 279
StyI CCWWGG 3 cut(s) 117, 125, 394
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 12
TaqI TCGA 1 cut(s) 214
TasI AATT 2 cut(s) 64, 295
TatI WGTACW 1 cut(s) 349
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TseI GCWGC 3 cut(s) 317, 326, 338
TspDTI ATGAA 3 cut(s) 99, 257, 279
TspGWI ACGGA 2 cut(s) 105, 298
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 125
XspI CTAG 3 cut(s) 36, 126, 279
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.