Rh3CG367700

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017817
Physical Location & Seq
Reverse (-)
46543864 .. 46547137
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG367700.1

Sequence Viewer

Length: 588 bp
ATGACTCTGCCGGGTCACCCGATGGTTCTTCCTCAGAAAATCCACCACCCCAAAACGGATACCACCGGCTTTGCCCGCCTTGATCTCCACCATGCAAGTACAAGTCACTTCGCGCAAAGCTGCTCACTTGAGCGACCAGCGGCTCATTTTGAGTTAGAAGCAAAATGTCAGAGCCTTGCAGGTGCTTTTTGTCGAGAGGGTCATCTTGGCTCATCAATCAGGATTAGAAGCTTCCCAACCTGCTCTAAATCAAATTGGGTGCTTGGTCCTACGATGAATGAATGCTCATGGATTGAATTACAACCACTCACTAGCCAAAAGCATCAGGTGTTGAATATCAATTGGTTTCAGGAGGCAACCAAGTGGGAACGACTAGAAAGTATTAATTTTCGGATGGCTGAGGAAAGCTTCATGGATCGCCTAGGCACCTATATGCATGAATCATGCCCCAAGATCGACAAAGATTGCTGGCAAGCAAACTATAATGAACCATTTCCAGATTCTTTGGTGTTTGTAGTCCATGGATCAAATGAGTCCACTCCGGTTGAAGATGCATTTGAGTTTGGCAAGATCATACCGGACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

195

Amino Acids

22.24

Weight (kDa)

5.92

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc36I ACCTGC 2 cut(s) 170, 248
AccB1I GGYRCC 1 cut(s) 425
AccII CGCG 1 cut(s) 113
AciI CCGC 2 cut(s) 76, 140
AclWI GGATC 2 cut(s) 423, 532
AfaI GTAC 1 cut(s) 100
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 3 cut(s) 296, 334, 548
AluBI AGCT 3 cut(s) 120, 231, 408
AluI AGCT 3 cut(s) 120, 231, 408
AlwI GGATC 2 cut(s) 423, 532
ApeKI GCWGC 1 cut(s) 120
AseI ATTAAT 1 cut(s) 384
Asp700I GAANNNNTTC 1 cut(s) 492
AspA2I CCTAGG 1 cut(s) 421
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 2 cut(s) 266, 580
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 8
AvaII GGWCC 2 cut(s) 266, 580
AvrII CCTAGG 1 cut(s) 421
BanI GGYRCC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 107
BccI CCATC 2 cut(s) 16, 388
BciVI GTATCC 1 cut(s) 52
BcnI CCSGG 1 cut(s) 12
BfaI CTAG 3 cut(s) 312, 374, 422
BfuAI ACCTGC 2 cut(s) 170, 248
BfuI GTATCC 1 cut(s) 52
BisI GCNGC 2 cut(s) 121, 141
BlnI CCTAGG 1 cut(s) 421
BlsI GCNGC 2 cut(s) 122, 142
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 2 cut(s) 266, 580
BmgT120I GGNCC 2 cut(s) 266, 580
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 2 cut(s) 331, 541
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 421, 520
BsaWI WCCGGW 2 cut(s) 541, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 421, 520
BseGI GGATG 1 cut(s) 399
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMII CTCAG 2 cut(s) 47, 390
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 113
BshNI GGYRCC 1 cut(s) 425
BsiSI CCGG 4 cut(s) 11, 66, 542, 578
BslI CCNNNNNNNGG 1 cut(s) 55
BsmI GAATGC 1 cut(s) 287
Bsp143I GATC 5 cut(s) 82, 415, 453, 524, 570
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 2 cut(s) 76, 140
BspCNI CTCAG 2 cut(s) 46, 391
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 427
BspMI ACCTGC 2 cut(s) 170, 248
BspPI GGATC 2 cut(s) 423, 532
BspT107I GGYRCC 1 cut(s) 425
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 421, 520
BssMI GATC 5 cut(s) 82, 415, 453, 524, 570
BssT1I CCWWGG 2 cut(s) 421, 520
BstC8I GCNNGC 3 cut(s) 76, 470, 474
BstDEI CTNAG 2 cut(s) 33, 399
BstDSI CCRYGG 1 cut(s) 520
BstEII GGTNACC 1 cut(s) 14
BstF5I GGATG 1 cut(s) 399
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 5 cut(s) 85, 418, 456, 527, 573
BstMBI GATC 5 cut(s) 82, 415, 453, 524, 570
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 10
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 107
BsuI GTATCC 1 cut(s) 52
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 399
BveI ACCTGC 2 cut(s) 170, 248
Cac8I GCNNGC 3 cut(s) 76, 470, 474
CfoI GCGC 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 266, 580
Csp6I GTAC 1 cut(s) 99
CspCI CAANNNNNGTGG 4 cut(s) 52, 87, 526, 561
CviAII CATG 6 cut(s) 92, 288, 412, 437, 444, 521
CviJI RGCY 9 cut(s) 69, 120, 143, 174, 210, 231, 315, 398, 408
CviKI_1 RGCY 9 cut(s) 69, 120, 143, 174, 210, 231, 315, 398, 408
CviQI GTAC 1 cut(s) 99
DdeI CTNAG 2 cut(s) 33, 399
DpnI GATC 5 cut(s) 84, 417, 455, 526, 572
DpnII GATC 5 cut(s) 82, 415, 453, 524, 570
Eco130I CCWWGG 2 cut(s) 421, 520
Eco47I GGWCC 2 cut(s) 266, 580
Eco91I GGTNACC 1 cut(s) 14
EcoO65I GGTNACC 1 cut(s) 14
EcoT14I CCWWGG 2 cut(s) 421, 520
EcoT22I ATGCAT 2 cut(s) 438, 556
ErhI CCWWGG 2 cut(s) 421, 520
FaeI CATG 6 cut(s) 95, 291, 415, 440, 447, 524
FatI CATG 6 cut(s) 91, 287, 411, 436, 443, 520
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 2 cut(s) 121, 141
FokI GGATG 1 cut(s) 406
Fsp4HI GCNGC 2 cut(s) 121, 141
FspBI CTAG 3 cut(s) 312, 374, 422
GlaI GCGC 1 cut(s) 114
GluI GCNGC 2 cut(s) 121, 141
HapII CCGG 4 cut(s) 11, 66, 542, 578
HhaI GCGC 1 cut(s) 115
Hin1II CATG 6 cut(s) 95, 291, 415, 440, 447, 524
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HindIII AAGCTT 2 cut(s) 229, 406
HinfI GANTC 4 cut(s) 4, 440, 500, 533
HpaII CCGG 4 cut(s) 11, 66, 542, 578
HphI GGTGA 1 cut(s) 8
Hpy166II GTNNAC 1 cut(s) 537
Hpy188I TCNGA 3 cut(s) 36, 171, 393
Hpy188III TCNNGA 4 cut(s) 194, 220, 350, 497
Hpy8I GTNNAC 1 cut(s) 537
HpyCH4V TGCA 4 cut(s) 95, 179, 436, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 2 cut(s) 33, 399
Hsp92II CATG 6 cut(s) 95, 291, 415, 440, 447, 524
HspAI GCGC 1 cut(s) 113
Kzo9I GATC 5 cut(s) 82, 415, 453, 524, 570
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 2 cut(s) 331, 541
MaeI CTAG 3 cut(s) 312, 374, 422
MaeIII GTNAC 2 cut(s) 14, 104
MalI GATC 5 cut(s) 84, 417, 455, 526, 572
MboI GATC 5 cut(s) 82, 415, 453, 524, 570
MboII GAAGA 2 cut(s) 20, 560
MfeI CAATTG 1 cut(s) 340
MluCI AATT 4 cut(s) 253, 296, 340, 385
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 4 cut(s) 42, 190, 346, 394
Mph1103I ATGCAT 2 cut(s) 438, 556
MroXI GAANNNNTTC 1 cut(s) 492
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 4 cut(s) 11, 66, 542, 578
MspR9I CCNGG 1 cut(s) 12
MunI CAATTG 1 cut(s) 340
Mva1269I GAATGC 1 cut(s) 287
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 75
NciI CCSGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 520
NdeII GATC 5 cut(s) 82, 415, 453, 524, 570
NlaIII CATG 6 cut(s) 95, 291, 415, 440, 447, 524
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 14, 104
NsiI ATGCAT 2 cut(s) 438, 556
PaqCI CACCTGC 1 cut(s) 170
PctI GAATGC 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 2 cut(s) 440, 500
PkrI GCNGC 2 cut(s) 122, 142
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PshBI ATTAAT 1 cut(s) 384
PspEI GGTNACC 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 2 cut(s) 266, 580
RsaI GTAC 1 cut(s) 100
RsaNI GTAC 1 cut(s) 99
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 121, 141
Sau3AI GATC 5 cut(s) 82, 415, 453, 524, 570
Sau96I GGNCC 2 cut(s) 266, 580
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 12
SetI ASST 7 cut(s) 122, 184, 233, 242, 330, 410, 431
SfaNI GCATC 2 cut(s) 331, 541
SinI GGWCC 2 cut(s) 266, 580
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 4 cut(s) 253, 296, 340, 385
SsiI CCGC 2 cut(s) 76, 140
SspMI CTAG 3 cut(s) 312, 374, 422
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 2 cut(s) 421, 520
TaqI TCGA 2 cut(s) 193, 456
TasI AATT 4 cut(s) 253, 296, 340, 385
TatI WGTACW 1 cut(s) 98
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 2 cut(s) 440, 500
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 2 cut(s) 14, 104
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 2 cut(s) 14, 104
TspDTI ATGAA 5 cut(s) 290, 294, 400, 453, 501
TspGWI ACGGA 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 266, 580
VspI ATTAAT 1 cut(s) 384
XmaJI CCTAGG 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 3 cut(s) 312, 374, 422
Zsp2I ATGCAT 2 cut(s) 438, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.