Rroxscaffold_1G00025720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013754
Physical Location & Seq
Forward (+)
32286442 .. 32308174
21733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00025720.1

Sequence Viewer

Length: 336 bp
ATGAGGTACCACATCTTCATGGCCGATCGGAACGATCCCAACGACAAAGGCCCCCTCCCCGCTGTCCGGATCAAAGACTCCGATGACGGCTTCACCGTCACGAGAGCCTTTCTAGGATTCGGGAAGGAAATGGTCAAGGTCGTCGTGGACTTCGAAAAGAACATCGTTAGCATCAAGCTTCGGGACTTGCGACGGCTGCGAAACCACGGGTTCCTCGCGCCGAAGGTTGTAAGAGCGGTGAGGCTAGGGCGGAGGGTTCCCCTCCTCCTTGAAGAGATATCTATATATAGTGGTGCAGCTGTGTGTTGTTGGTCTCTGAAGGGAGAGAAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.59

Weight (kDa)

9.94

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 6
AccB1I GGYRCC 1 cut(s) 6
AccBSI CCGCTC 1 cut(s) 236
AccII CGCG 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 66
AciI CCGC 3 cut(s) 60, 236, 250
AclWI GGATC 2 cut(s) 29, 77
AcoI YGGCCR 1 cut(s) 21
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 66
AgsI TTSAA 1 cut(s) 272
AluBI AGCT 2 cut(s) 178, 299
AluI AGCT 2 cut(s) 178, 299
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 2 cut(s) 29, 77
Aor13HI TCCGGA 1 cut(s) 66
AoxI GGCC 2 cut(s) 21, 49
ApeKI GCWGC 2 cut(s) 196, 296
Asp718I GGTACC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 85, 250
AsuII TTCGAA 1 cut(s) 153
BanI GGYRCC 1 cut(s) 6
BarI GAAGNNNNNNTAC 1 cut(s) 31
BauI CACGAG 1 cut(s) 100
BbvI GCAGC 2 cut(s) 183, 308
BceAI ACGGC 2 cut(s) 103, 209
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 2 cut(s) 113, 245
BisI GCNGC 2 cut(s) 197, 297
BlsI GCNGC 2 cut(s) 198, 298
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 4 cut(s) 8, 52, 212, 258
BmsI GCATC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 66
Bsc4I CCNNNNNNNGG 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 2 cut(s) 183, 308
BsgI GTGCAG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 23, 51
BshNI GGYRCC 1 cut(s) 6
BsiEI CGRYCG 1 cut(s) 28
BsiSI CCGG 1 cut(s) 67
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 23, 51
Bso31I GGTCTC 1 cut(s) 318
Bsp119I TTCGAA 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 66
Bsp143I GATC 3 cut(s) 25, 34, 69
BspACI CCGC 3 cut(s) 60, 236, 250
BspANI GGCC 2 cut(s) 23, 51
BspEI TCCGGA 1 cut(s) 66
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 4 cut(s) 8, 52, 212, 258
BspPI GGATC 2 cut(s) 29, 77
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 318
BsrBI CCGCTC 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 3 cut(s) 25, 34, 69
BssSI CACGAG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 100
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 205
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 3 cut(s) 28, 37, 72
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 3 cut(s) 25, 34, 69
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 183, 308
BsuRI GGCC 2 cut(s) 23, 51
BtgI CCRYGG 1 cut(s) 205
CfoI GCGC 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 7
CviAII CATG 1 cut(s) 19
CviJI RGCY 8 cut(s) 23, 51, 90, 107, 178, 196, 244, 299
CviKI_1 RGCY 8 cut(s) 23, 51, 90, 107, 178, 196, 244, 299
CviQI GTAC 1 cut(s) 7
DpnI GATC 3 cut(s) 27, 36, 71
DpnII GATC 3 cut(s) 25, 34, 69
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
EciI GGCGGA 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 279
EcoO109I RGGNCCY 1 cut(s) 50
EcoRV GATATC 1 cut(s) 279
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 20, 284, 286, 288
FaqI GGGAC 1 cut(s) 197
FatI CATG 1 cut(s) 18
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 197, 297
Fsp4HI GCNGC 2 cut(s) 197, 297
FspBI CTAG 2 cut(s) 113, 245
GlaI GCGC 1 cut(s) 219
GluI GCNGC 2 cut(s) 197, 297
HaeIII GGCC 2 cut(s) 23, 51
HapII CCGG 1 cut(s) 67
HhaI GCGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 2 cut(s) 77, 117
HpaII CCGG 1 cut(s) 67
HphI GGTGA 2 cut(s) 85, 250
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 3 cut(s) 30, 82, 318
Hpy188III TCNNGA 4 cut(s) 67, 100, 121, 182
Hpy8I GTNNAC 1 cut(s) 148
Hpy99I CGWCG 2 cut(s) 146, 195
HpyAV CCTTC 3 cut(s) 118, 217, 313
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 66
KpnI GGTACC 1 cut(s) 10
Kzo9I GATC 3 cut(s) 25, 34, 69
LpnPI CCDG 1 cut(s) 80
Lsp1109I GCAGC 2 cut(s) 183, 308
LweI GCATC 1 cut(s) 180
MaeI CTAG 2 cut(s) 113, 245
MaeIII GTNAC 1 cut(s) 97
MalI GATC 3 cut(s) 27, 36, 71
MbiI CCGCTC 1 cut(s) 236
MboI GATC 3 cut(s) 25, 34, 69
MboII GAAGA 2 cut(s) 7, 284
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 6 cut(s) 65, 224, 234, 246, 272, 275
MroI TCCGGA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 62, 299
MspI CCGG 1 cut(s) 67
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 196
NdeII GATC 3 cut(s) 25, 34, 69
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 4 cut(s) 8, 52, 212, 258
NmuCI GTSAC 1 cut(s) 97
NspV TTCGAA 1 cut(s) 153
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 93, 150, 187
PfeI GAWTC 1 cut(s) 117
PkrI GCNGC 2 cut(s) 198, 298
Ple19I CGATCG 1 cut(s) 28
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PspN4I GGNNCC 4 cut(s) 8, 52, 212, 258
PspPI GGNCC 1 cut(s) 50
PvuI CGATCG 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 17
SatI GCNGC 2 cut(s) 197, 297
Sau3AI GATC 3 cut(s) 25, 34, 69
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 1 cut(s) 71
SetI ASST 5 cut(s) 8, 141, 180, 228, 301
SfaNI GCATC 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 17
SsiI CCGC 3 cut(s) 60, 236, 250
SspMI CTAG 2 cut(s) 113, 245
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 1 cut(s) 153
TfiI GAWTC 1 cut(s) 117
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 196, 296
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 7
XspI CTAG 2 cut(s) 113, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.