Rroxscaffold_4G00283550

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013754
Physical Location & Seq
Reverse (-)
5462554 .. 5463258
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283550.1

Sequence Viewer

Length: 414 bp
ATGGCGTTTCCACTCTCTCGAACCCTCAGGCGAGCCCTCATCTCCGGCGACAACCTCTTCCCGGCCACCCTTGCCCGATCAATCTCCACCACCGCCTCTTCCGCCATGAGGTACCACATCTTCATGGCCGATCCGAACGATCCCAACGACAAAGGCCCCCTCCCCGCCTTCCAGATCAAAGACTCCGACGAAAGCTTCACCGTCACAAGAGCCTTTCCGGGATTCGGGAAGGAAATCGTCAAGGTCGAGGTGGACGTCGAGAAGAACACCGTCAGCATCAAGCTCCGGGACTGCGACGGCTGCGAAACCCTGGTTCCTCTGCCGGAGCGTTGCAAGAGCGGCGAGGCTAGGGCGGAGGTTAAGCATGGTATGGTGACGGTGGTTGTGCCGAAGCGGAAGAAAGGAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

137

Amino Acids

15.08

Weight (kDa)

8.47

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AccBSI CCGCTC 1 cut(s) 339
AciI CCGC 6 cut(s) 93, 102, 165, 339, 353, 394
AclWI GGATC 2 cut(s) 125, 134
AcoI YGGCCR 2 cut(s) 63, 126
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 3 cut(s) 61, 108, 224
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 2 cut(s) 195, 283
AluI AGCT 2 cut(s) 195, 283
AlwI GGATC 2 cut(s) 125, 134
AoxI GGCC 3 cut(s) 63, 126, 154
ApeKI GCWGC 1 cut(s) 300
Asp718I GGTACC 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 155
AsuC2I CCSGG 3 cut(s) 62, 219, 287
AsuHPI GGTGA 2 cut(s) 190, 385
AxyI CCTNAGG 1 cut(s) 26
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 37
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbvI GCAGC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 311
BcnI CCSGG 3 cut(s) 62, 219, 287
BfaI CTAG 1 cut(s) 348
BisI GCNGC 2 cut(s) 301, 340
BlsI GCNGC 2 cut(s) 302, 341
Bme1390I CCNGG 4 cut(s) 62, 219, 287, 311
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 3 cut(s) 113, 157, 315
BmrFI CCNGG 4 cut(s) 62, 219, 287, 311
BmsI GCATC 1 cut(s) 285
BpuMI CCSGG 3 cut(s) 62, 219, 287
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 108, 224
Bse21I CCTNAGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 309
BseLI CCNNNNNNNGG 3 cut(s) 61, 108, 224
BseMII CTCAG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 287
BshFI GGCC 3 cut(s) 65, 128, 156
BshNI GGYRCC 1 cut(s) 111
BsiSI CCGG 5 cut(s) 45, 62, 218, 286, 323
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 3 cut(s) 61, 108, 224
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 3 cut(s) 65, 128, 156
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 4 cut(s) 77, 130, 139, 174
BspACI CCGC 6 cut(s) 93, 102, 165, 339, 353, 394
BspANI GGCC 3 cut(s) 65, 128, 156
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 113, 157, 315
BspPI GGATC 2 cut(s) 125, 134
BspT107I GGYRCC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 77, 130, 139, 174
BssNI GRCGYC 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 202, 271, 379
Bst6I CTCTTC 2 cut(s) 62, 103
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 1 cut(s) 26
BstKTI GATC 4 cut(s) 80, 133, 142, 177
BstMBI GATC 4 cut(s) 77, 130, 139, 174
BstMWI GCNNNNNNNGC 4 cut(s) 71, 101, 300, 339
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 60, 217, 285, 309
BstV1I GCAGC 1 cut(s) 287
Bsu36I CCTNAGG 1 cut(s) 26
BsuRI GGCC 3 cut(s) 65, 128, 156
Cac8I GCNNGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 112
CviAII CATG 3 cut(s) 106, 124, 365
CviJI RGCY 9 cut(s) 35, 65, 128, 156, 195, 212, 283, 300, 347
CviKI_1 RGCY 9 cut(s) 35, 65, 128, 156, 195, 212, 283, 300, 347
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 26
DpnI GATC 4 cut(s) 79, 132, 141, 176
DpnII GATC 4 cut(s) 77, 130, 139, 174
EaeI YGGCCR 2 cut(s) 63, 126
Eam1104I CTCTTC 2 cut(s) 62, 103
EarI CTCTTC 2 cut(s) 62, 103
EciI GGCGGA 2 cut(s) 91, 368
Eco24I GRGCYC 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 37
FaeI CATG 3 cut(s) 109, 127, 368
FaiI YATR 4 cut(s) 107, 125, 366, 371
FaqI GGGAC 1 cut(s) 302
FatI CATG 3 cut(s) 105, 123, 364
FauI CCCGC 1 cut(s) 172
Fnu4HI GCNGC 2 cut(s) 301, 340
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 2 cut(s) 301, 340
FspBI CTAG 1 cut(s) 348
GluI GCNGC 2 cut(s) 301, 340
HaeIII GGCC 3 cut(s) 65, 128, 156
HapII CCGG 5 cut(s) 45, 62, 218, 286, 323
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 3 cut(s) 109, 127, 368
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 2 cut(s) 182, 222
HpaII CCGG 5 cut(s) 45, 62, 218, 286, 323
HphI GGTGA 2 cut(s) 190, 385
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 2 cut(s) 135, 187
Hpy188III TCNNGA 4 cut(s) 18, 172, 226, 259
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 3 cut(s) 191, 260, 299
HpyAV CCTTC 2 cut(s) 178, 223
HpyCH4III ACNGT 3 cut(s) 202, 271, 379
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 101, 300, 339
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 3 cut(s) 109, 127, 368
KpnI GGTACC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 77, 130, 139, 174
LmnI GCTCC 2 cut(s) 288, 325
LpnPI CCDG 9 cut(s) 13, 58, 75, 185, 231, 296, 299, 323, 336
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 285
MaeI CTAG 1 cut(s) 348
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 202, 373
MalI GATC 4 cut(s) 79, 132, 141, 176
MbiI CCGCTC 1 cut(s) 339
MboI GATC 4 cut(s) 77, 130, 139, 174
MboII GAAGA 5 cut(s) 49, 90, 112, 274, 409
MhlI GDGCHC 1 cut(s) 37
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 210
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 122
MspI CCGG 5 cut(s) 45, 62, 218, 286, 323
MspR9I CCNGG 4 cut(s) 62, 219, 287, 311
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 71, 101, 300, 339
NciI CCSGG 3 cut(s) 62, 219, 287
NdeII GATC 4 cut(s) 77, 130, 139, 174
NlaIII CATG 3 cut(s) 109, 127, 368
NlaIV GGNNCC 3 cut(s) 113, 157, 315
NmuCI GTSAC 2 cut(s) 202, 373
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 243, 252
PfeI GAWTC 1 cut(s) 222
PfoI TCCNGGA 2 cut(s) 217, 285
PkrI GCNGC 2 cut(s) 302, 341
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 113, 157, 315
PspPI GGNCC 1 cut(s) 155
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 2 cut(s) 301, 340
Sau3AI GATC 4 cut(s) 77, 130, 139, 174
Sau96I GGNCC 1 cut(s) 155
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 4 cut(s) 62, 219, 287, 311
SduI GDGCHC 1 cut(s) 37
SetI ASST 8 cut(s) 57, 113, 197, 246, 252, 258, 285, 360
SfaNI GCATC 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 122
SsiI CCGC 6 cut(s) 93, 102, 165, 339, 353, 394
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 4 cut(s) 60, 217, 285, 309
TaaI ACNGT 3 cut(s) 202, 271, 379
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 19, 246, 258
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TseFI GTSAC 2 cut(s) 202, 373
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 2 cut(s) 202, 373
TspDTI ATGAA 1 cut(s) 112
XspI CTAG 1 cut(s) 348
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.