Rroxscaffold_2G00086220

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016376
Physical Location & Seq
Forward (+)
8454263 .. 8456071
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086220.1

Sequence Viewer

Length: 609 bp
ATGGCTGCAAATCATCCCTTCCGAGTTCCGAAAAAGCCTTGCACAGTCGACATCGTTTCGGATCCGGTATGCCCTTGGTGCTTTATAGGCAAGAAAAATCTTGACAAAGCTCTAGAGGAGGCTGACCCTCTGTACGTGTTTGAGCTCCGGTGGCATCCATTTCAAACTTATCCTGATATCCCTAAAGAAGGCATTGATAAGAAAAAACATTCTGAGGATAAGCATGGCAGCACTCATATATCAGAAGTTGTGGAAATGCGTATAGGAGATATTTTGAGAACCACCTACGATGCGCTTGAATACAAACTTTCTGGAATCATGGGAAATCCTCTAGATTATCACAGGCTTGTATATTTTGCTGGGGAACAGGATCATGATATGCAACATGATCTTGTGGATGAGATACTGCTTGGGTATTTCACAGAGGAAAAGGACATTGCAGACAGGGAATATCTTGTGGAATGTGCTGGTAAGATTGGCATAGAAGGGGCAGCAGAGTTTCTTGAAGACCCTAACAATGGGCTCAATGAGTTTAATGGAAAGGTGGACTGCGTCGGTGCTCAGCCGTCTGAGGTGTTCTTGAGAGCTTTCGAAGCAGCCACAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

202

Amino Acids

22.93

Weight (kDa)

4.83

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 15 - 172 1.2e-18 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AclWI GGATC 3 cut(s) 56, 69, 378
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 188, 518
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 3 cut(s) 164, 299, 506
AluBI AGCT 3 cut(s) 110, 145, 587
AluI AGCT 3 cut(s) 110, 145, 587
Alw21I GWGCWC 2 cut(s) 147, 562
AlwI GGATC 3 cut(s) 56, 69, 378
ApeKI GCWGC 4 cut(s) 5, 228, 491, 596
AspLEI GCGC 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 591
BamHI GGATCC 1 cut(s) 61
BanII GRGCYC 2 cut(s) 147, 525
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 147, 562
BbvI GCAGC 2 cut(s) 240, 503
BceAI ACGGC 1 cut(s) 550
BfaI CTAG 2 cut(s) 113, 332
BisI GCNGC 4 cut(s) 6, 229, 492, 597
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 4 cut(s) 7, 230, 493, 598
BmiI GGNNCC 1 cut(s) 63
BmsI GCATC 2 cut(s) 163, 280
BpiI GAAGAC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 601
BsaAI YACGTR 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 74
BsaWI WCCGGW 2 cut(s) 64, 147
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 518
Bse3DI GCAATG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 3 cut(s) 13, 154, 403
BseLI CCNNNNNNNGG 2 cut(s) 188, 518
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 3 cut(s) 204, 561, 575
BseRI GAGGAG 1 cut(s) 131
BseXI GCAGC 2 cut(s) 240, 503
BseYI CCCAGC 1 cut(s) 359
BsiHKAI GWGCWC 2 cut(s) 147, 562
BsiSI CCGG 2 cut(s) 65, 148
BslI CCNNNNNNNGG 2 cut(s) 188, 518
Bsp119I TTCGAA 1 cut(s) 591
Bsp1286I GDGCHC 3 cut(s) 147, 525, 562
Bsp143I GATC 3 cut(s) 61, 370, 388
Bsp1720I GCTNAGC 1 cut(s) 561
BspCNI CTCAG 3 cut(s) 205, 562, 574
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 63
BspPI GGATC 3 cut(s) 56, 69, 378
BspT104I TTCGAA 1 cut(s) 591
BsrDI GCAATG 1 cut(s) 435
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 3 cut(s) 61, 370, 388
BssT1I CCWWGG 1 cut(s) 74
Bst4CI ACNGT 1 cut(s) 46
BstBAI YACGTR 1 cut(s) 136
BstBI TTCGAA 1 cut(s) 591
BstDEI CTNAG 3 cut(s) 213, 561, 570
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 13, 154, 403
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 3 cut(s) 64, 373, 391
BstMBI GATC 3 cut(s) 61, 370, 388
BstMWI GCNNNNNNNGC 4 cut(s) 78, 87, 151, 593
BstV1I GCAGC 2 cut(s) 240, 503
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BtsCI GGATG 3 cut(s) 13, 154, 403
CciI TCATGA 1 cut(s) 373
CfoI GCGC 1 cut(s) 295
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 133
CviAII CATG 4 cut(s) 224, 319, 374, 386
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 3 cut(s) 213, 561, 570
DpnI GATC 3 cut(s) 63, 372, 390
DpnII GATC 3 cut(s) 61, 370, 388
Ecl136II GAGCTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 2 cut(s) 147, 525
Eco32I GATATC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 74
EcoT38I GRGCYC 2 cut(s) 147, 525
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 4 cut(s) 227, 322, 377, 389
FatI CATG 4 cut(s) 223, 318, 373, 385
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 6, 229, 492, 597
FokI GGATG 2 cut(s) 141, 410
FriOI GRGCYC 2 cut(s) 147, 525
Fsp4HI GCNGC 4 cut(s) 6, 229, 492, 597
FspBI CTAG 2 cut(s) 113, 332
GlaI GCGC 1 cut(s) 294
GluI GCNGC 4 cut(s) 6, 229, 492, 597
GsaI CCCAGC 1 cut(s) 363
HapII CCGG 2 cut(s) 65, 148
HgaI GACGC 1 cut(s) 541
HhaI GCGC 1 cut(s) 295
Hin1II CATG 4 cut(s) 227, 322, 377, 389
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 1 cut(s) 315
HpaII CCGG 2 cut(s) 65, 148
Hpy166II GTNNAC 2 cut(s) 49, 547
Hpy188I TCNGA 6 cut(s) 23, 30, 61, 214, 244, 571
Hpy188III TCNNGA 8 cut(s) 101, 113, 173, 312, 332, 374, 503, 580
Hpy8I GTNNAC 2 cut(s) 49, 547
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 3 cut(s) 28, 182, 479
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 8, 42, 382, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 87, 151, 593
HpyF3I CTNAG 3 cut(s) 213, 561, 570
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 4 cut(s) 227, 322, 377, 389
HspAI GCGC 1 cut(s) 293
Kzo9I GATC 3 cut(s) 61, 370, 388
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 9 cut(s) 78, 161, 186, 297, 328, 345, 353, 430, 453
Lsp1109I GCAGC 2 cut(s) 240, 503
LweI GCATC 2 cut(s) 163, 280
MaeI CTAG 2 cut(s) 113, 332
MaeII ACGT 1 cut(s) 135
MalI GATC 3 cut(s) 63, 372, 390
MboI GATC 3 cut(s) 61, 370, 388
MboII GAAGA 1 cut(s) 518
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 3 cut(s) 147, 525, 562
MnlI CCTC 7 cut(s) 109, 112, 138, 208, 339, 418, 565
MseI TTAA 1 cut(s) 534
MspI CCGG 2 cut(s) 65, 148
MwoI GCNNNNNNNGC 4 cut(s) 78, 87, 151, 593
NdeII GATC 3 cut(s) 61, 370, 388
NlaIII CATG 4 cut(s) 227, 322, 377, 389
NlaIV GGNNCC 1 cut(s) 63
NspV TTCGAA 1 cut(s) 591
PagI TCATGA 1 cut(s) 373
PfeI GAWTC 1 cut(s) 315
PflFI GACNNNGTC 1 cut(s) 551
PkrI GCNGC 4 cut(s) 7, 230, 493, 598
Ppu21I YACGTR 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 63
PsuI RGATCY 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SacI GAGCTC 1 cut(s) 147
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 4 cut(s) 6, 229, 492, 597
Sau3AI GATC 3 cut(s) 61, 370, 388
SduI GDGCHC 3 cut(s) 147, 525, 562
SetI ASST 7 cut(s) 112, 138, 147, 287, 546, 576, 589
SfaNI GCATC 2 cut(s) 163, 280
SfuI TTCGAA 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 580
SmoI CTYRAG 1 cut(s) 580
SspMI CTAG 2 cut(s) 113, 332
SstI GAGCTC 1 cut(s) 147
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 48, 591
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TseI GCWGC 4 cut(s) 5, 228, 491, 596
Tth111I GACNNNGTC 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 186
XbaI TCTAGA 2 cut(s) 112, 331
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 113, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.