Rroxscaffold_2G00105510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019048
Physical Location & Seq
Forward (+)
28914907 .. 28921362
6456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105510.1

Sequence Viewer

Length: 489 bp
ATGAGAGAGATATCAGCCGGTGAAGGAAGAGCAGCTCGGGCGAATTCCAGCTCGAGCTGTGCAGCTTCTCGGCCTCCTAGGGCCTTCCACGCCGGGGAGTTTGGCGCTGGTGATGACATCATCTTTGAGGCCGGAGAGGGAGAAGCGAGGGCTGCGCTGCCGCGTGGGATTGGAACGATGCGGGCTGGTCACCGACTCGGACCAAGCCGGGCACGTTTGAAATCGGATCTGTTGGGTAGATTTGGCCCGATGGCTGGGTTGATGGGCTGTTGCTTGACCATGTTCTCGGGTGATGGGAAGAGGCCGGATTGGGGGCCGGAGAGAATTGGCTTTGGCGTTCGGTTCCTCGTGGTTTTGACCGGAGGGGATCTAGTTTGGAGTAGAATCTCTTCGGTTCTACGTCGAAATCGCTTCAACAACGGTGGGGAAGGCTGTGACTCTTTGTTTCAAGCGGATGGGTCGGGATGGGCTTTCTCCGGCGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.1

Weight (kDa)

8.93

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 163
AciI CCGC 3 cut(s) 161, 181, 452
AclWI GGATC 2 cut(s) 234, 375
AcsI RAATTY 1 cut(s) 43
AfiI CCNNNNNNNGG 3 cut(s) 94, 254, 311
AgsI TTSAA 3 cut(s) 220, 415, 449
AluBI AGCT 4 cut(s) 35, 51, 57, 65
AluI AGCT 4 cut(s) 35, 51, 57, 65
AlwI GGATC 2 cut(s) 234, 375
Ama87I CYCGRG 3 cut(s) 36, 52, 286
AoxI GGCC 6 cut(s) 71, 81, 129, 244, 302, 314
ApeKI GCWGC 4 cut(s) 32, 62, 152, 157
ApoI RAATTY 1 cut(s) 43
ArsI GACNNNNNNTTYG 2 cut(s) 107, 139
Asp700I GAANNNNTTC 1 cut(s) 388
AspA2I CCTAGG 1 cut(s) 77
AspLEI GCGC 2 cut(s) 107, 157
AspS9I GGNCC 4 cut(s) 81, 200, 245, 314
AsuC2I CCSGG 2 cut(s) 94, 209
AsuHPI GGTGA 4 cut(s) 32, 122, 182, 302
AvaI CYCGRG 3 cut(s) 36, 52, 286
AvaII GGWCC 1 cut(s) 200
AvrII CCTAGG 1 cut(s) 77
BaeGI GKGCMC 1 cut(s) 214
BarI GAAGNNNNNNTAC 2 cut(s) 373, 405
BauI CACGAG 1 cut(s) 347
BbvI GCAGC 4 cut(s) 44, 74, 139, 144
BccI CCATC 5 cut(s) 244, 256, 287, 449, 459
BcnI CCSGG 2 cut(s) 94, 209
BfaI CTAG 2 cut(s) 78, 371
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 5 cut(s) 33, 63, 153, 158, 161
BlnI CCTAGG 1 cut(s) 77
BlsI GCNGC 5 cut(s) 34, 64, 154, 159, 162
Bme1390I CCNGG 2 cut(s) 94, 209
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 3 cut(s) 36, 52, 286
BmgT120I GGNCC 4 cut(s) 81, 200, 245, 314
BmiI GGNNCC 2 cut(s) 315, 344
BmrFI CCNGG 2 cut(s) 94, 209
BmsI GCATC 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 94, 209
BsaJI CCNNGG 2 cut(s) 77, 93
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 254, 311
Bse118I RCCGGY 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 77, 93
BseGI GGATG 2 cut(s) 460, 470
BseLI CCNNNNNNNGG 3 cut(s) 94, 254, 311
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 4 cut(s) 44, 74, 139, 144
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 163
BshFI GGCC 6 cut(s) 73, 83, 131, 246, 304, 316
BsiHKCI CYCGRG 3 cut(s) 36, 52, 286
BsiSI CCGG 8 cut(s) 18, 93, 132, 208, 305, 317, 360, 477
BslI CCNNNNNNNGG 3 cut(s) 94, 254, 311
BsnI GGCC 6 cut(s) 73, 83, 131, 246, 304, 316
BsoBI CYCGRG 3 cut(s) 36, 52, 286
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 2 cut(s) 226, 367
BspACI CCGC 3 cut(s) 161, 181, 452
BspANI GGCC 6 cut(s) 73, 83, 131, 246, 304, 316
BspFNI CGCG 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 315, 344
BspPI GGATC 2 cut(s) 234, 375
BspQI GCTCTTC 1 cut(s) 22
BsrFI RCCGGY 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 77, 93
BssMI GATC 2 cut(s) 226, 367
BssSI CACGAG 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 422
Bst6I CTCTTC 3 cut(s) 22, 293, 394
BstC8I GCNNGC 1 cut(s) 183
BstEII GGTNACC 1 cut(s) 188
BstF5I GGATG 2 cut(s) 460, 470
BstFNI CGCG 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 2 cut(s) 107, 157
BstKTI GATC 2 cut(s) 229, 370
BstMBI GATC 2 cut(s) 226, 367
BstMWI GCNNNNNNNGC 3 cut(s) 38, 89, 152
BstPI GGTNACC 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 92, 207
BstSLI GKGCMC 1 cut(s) 214
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 4 cut(s) 44, 74, 139, 144
BstX2I RGATCY 2 cut(s) 226, 367
BstYI RGATCY 2 cut(s) 226, 367
BsuRI GGCC 6 cut(s) 73, 83, 131, 246, 304, 316
BtsCI GGATG 2 cut(s) 460, 470
Cac8I GCNNGC 1 cut(s) 183
CfoI GCGC 2 cut(s) 107, 157
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 4 cut(s) 81, 200, 245, 314
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 1 cut(s) 280
DpnI GATC 2 cut(s) 228, 369
DpnII GATC 2 cut(s) 226, 367
Eam1104I CTCTTC 3 cut(s) 22, 293, 394
EarI CTCTTC 3 cut(s) 22, 293, 394
Eco130I CCWWGG 1 cut(s) 77
Eco32I GATATC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 200
Eco88I CYCGRG 3 cut(s) 36, 52, 286
Eco91I GGTNACC 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 81
EcoO65I GGTNACC 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 43
EcoRV GATATC 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 1 cut(s) 283
FaiI YATR 1 cut(s) 281
FatI CATG 1 cut(s) 279
FauI CCCGC 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 33, 63, 153, 158, 161
FokI GGATG 2 cut(s) 467, 477
Fsp4HI GCNGC 5 cut(s) 33, 63, 153, 158, 161
FspBI CTAG 2 cut(s) 78, 371
GlaI GCGC 2 cut(s) 106, 156
GluI GCNGC 5 cut(s) 33, 63, 153, 158, 161
GsaI CCCAGC 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 6 cut(s) 73, 83, 131, 246, 304, 316
HapII CCGG 8 cut(s) 18, 93, 132, 208, 305, 317, 360, 477
HhaI GCGC 2 cut(s) 107, 157
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 2 cut(s) 105, 155
HinP1I GCGC 2 cut(s) 105, 155
HinfI GANTC 3 cut(s) 195, 384, 437
HpaII CCGG 8 cut(s) 18, 93, 132, 208, 305, 317, 360, 477
HphI GGTGA 4 cut(s) 32, 122, 182, 302
Hpy188I TCNGA 2 cut(s) 200, 226
Hpy188III TCNNGA 1 cut(s) 462
Hpy99I CGWCG 1 cut(s) 405
HpyAV CCTTC 3 cut(s) 17, 94, 422
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4IV ACGT 2 cut(s) 214, 400
HpyCH4V TGCA 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 89, 152
HpySE526I ACGT 2 cut(s) 214, 400
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 2 cut(s) 105, 155
Kzo9I GATC 2 cut(s) 226, 367
LguI GCTCTTC 1 cut(s) 22
Lsp1109I GCAGC 4 cut(s) 44, 74, 139, 144
LweI GCATC 1 cut(s) 168
MaeI CTAG 2 cut(s) 78, 371
MaeII ACGT 2 cut(s) 214, 400
MaeIII GTNAC 2 cut(s) 188, 434
MalI GATC 2 cut(s) 228, 369
MboI GATC 2 cut(s) 226, 367
MboII GAAGA 3 cut(s) 39, 310, 381
MflI RGATCY 2 cut(s) 226, 367
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 2 cut(s) 43, 324
MlyI GAGTC 2 cut(s) 189, 431
MnlI CCTC 8 cut(s) 84, 121, 130, 141, 294, 356, 356, 475
MroXI GAANNNNTTC 1 cut(s) 388
MspI CCGG 8 cut(s) 18, 93, 132, 208, 305, 317, 360, 477
MspR9I CCNGG 2 cut(s) 94, 209
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 3 cut(s) 38, 89, 152
NciI CCSGG 2 cut(s) 94, 209
NdeII GATC 2 cut(s) 226, 367
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 2 cut(s) 315, 344
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 188, 434
PaeR7I CTCGAG 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 384
PkrI GCNGC 5 cut(s) 34, 64, 154, 159, 162
PleI GAGTC 2 cut(s) 189, 431
PpsI GAGTC 2 cut(s) 189, 431
PspEI GGTNACC 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 2 cut(s) 315, 344
PspPI GGNCC 4 cut(s) 81, 200, 245, 314
PspXI VCTCGAGB 1 cut(s) 52
PsuI RGATCY 2 cut(s) 226, 367
SapI GCTCTTC 1 cut(s) 22
SatI GCNGC 5 cut(s) 33, 63, 153, 158, 161
Sau3AI GATC 2 cut(s) 226, 367
Sau96I GGNCC 4 cut(s) 81, 200, 245, 314
SchI GAGTC 2 cut(s) 189, 431
ScrFI CCNGG 2 cut(s) 94, 209
SduI GDGCHC 1 cut(s) 214
SetI ASST 6 cut(s) 37, 53, 59, 67, 217, 403
SfaNI GCATC 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 52
SinI GGWCC 1 cut(s) 200
SlaI CTCGAG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 43, 324
SsiI CCGC 3 cut(s) 161, 181, 452
SspMI CTAG 2 cut(s) 78, 371
StyD4I CCNGG 2 cut(s) 92, 207
StyI CCWWGG 1 cut(s) 77
TaaI ACNGT 1 cut(s) 422
TaiI ACGT 2 cut(s) 217, 403
TaqI TCGA 2 cut(s) 53, 403
TasI AATT 2 cut(s) 43, 324
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 1 cut(s) 384
TseFI GTSAC 2 cut(s) 188, 434
TseI GCWGC 4 cut(s) 32, 62, 152, 157
Tsp45I GTSAC 2 cut(s) 188, 434
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 1 cut(s) 43
XhoI CTCGAG 1 cut(s) 52
XmaJI CCTAGG 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 2 cut(s) 78, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.