Rroxscaffold_2G00105560
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015145
Physical Location & Seq
Reverse (-)
28978915 .. 28979823
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105560.1

Sequence Viewer

Length: 471 bp
ATGGAAGACGAGAAGCTGCAGAAGTCCAAGAAATGGCTTCACGATCTGTCGACAGGTGCTAAAGGTATCAACGAGCTAGAGGCCTTAACTCATGTTGGCCTGCAGGTCATGAATGCCCGCAAGGGCTTCTTCCGCTGCAACTTCATCGTTCCGGATCATCTCTCTGACCAGGATGGAAACTGGCATGTCGGAGCTATCGCAACTGTGATCGACGATGTTGGGGCCGCCACCGTATACTCAACTGTTGGTGATGTCAAATCCGTTGACCTCACCATTTCATATTACTCCAGGGTCAAGACTAGAGAAGAGGTTGAGCTGGAGGCTGAGATTGTAGGGGACATGGGGAAGCTCATATGTGTGGTGGTAAAGGTGACAAGAAAAGAGAATGGAGAAAGGGTTGCTTTGGGTAAGCAATGGATGGCCTCATATTCAATGAGGACCAATCAATCTCAAGTCACTAGTAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

156

Amino Acids

17.39

Weight (kDa)

6.42

Isoelectric Point (pI)

13.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 59 - 131 2.4e-06 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52191 AT3G16175
fragaria_vesca FvH4_6g31870 FvH4_6g31870
rosa_chinensis RchiOBHm_Chr2g0139201
rosa_laevigata RLG00000019794
rosa_multiflora Rmu_sc0001803.1_g000007 Rmu_sc0037230.1_g000003
rosa_roxburghii Rroxscaffold_2G00105560
rosa_rugosa Rorug02G0355500 Rorug02G0355500
rosa_samantha Rh2AG405700 Rh2BG416200 Rh2CG391700 Rh2DG425400
rosa_wichuraiana Rw2G033130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 33
AccI GTMKAC 2 cut(s) 50, 234
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 3 cut(s) 118, 133, 225
AclWI GGATC 1 cut(s) 162
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 1 cut(s) 432
AhlI ACTAGT 1 cut(s) 458
AjnI CCWGG 2 cut(s) 168, 287
AluBI AGCT 5 cut(s) 16, 76, 194, 316, 349
AluI AGCT 5 cut(s) 16, 76, 194, 316, 349
AlwI GGATC 1 cut(s) 162
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 4 cut(s) 81, 97, 222, 420
ApeKI GCWGC 2 cut(s) 16, 135
AspS9I GGNCC 2 cut(s) 222, 438
AsuHPI GGTGA 3 cut(s) 260, 262, 382
AvaII GGWCC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 3, 122
BccI CCATC 2 cut(s) 167, 412
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 2 cut(s) 170, 289
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 3 cut(s) 77, 300, 459
BfmI CTRYAG 2 cut(s) 17, 101
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 3 cut(s) 17, 136, 225
BlsI GCNGC 3 cut(s) 18, 137, 226
Bme1390I CCNGG 2 cut(s) 170, 289
Bme18I GGWCC 1 cut(s) 438
BmgT120I GGNCC 2 cut(s) 222, 438
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 2 cut(s) 170, 289
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 271, 338
BpuEI CTTGAG 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse1I ACTGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 419
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 2 cut(s) 170, 289
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 2 cut(s) 178, 423
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 3, 122
BshFI GGCC 4 cut(s) 83, 99, 224, 422
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 4 cut(s) 83, 99, 224, 422
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 3 cut(s) 43, 154, 207
BspACI CCGC 3 cut(s) 118, 133, 225
BspANI GGCC 4 cut(s) 83, 99, 224, 422
BspCNI CTCAG 1 cut(s) 316
BspEI TCCGGA 1 cut(s) 151
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 223
BspMAI CTGCAG 2 cut(s) 21, 105
BspMI ACCTGC 1 cut(s) 94
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 419
BsrI ACTGG 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 3 cut(s) 43, 154, 207
BssNAI GTATAC 1 cut(s) 235
Bst1107I GTATAC 1 cut(s) 235
Bst2UI CCWGG 2 cut(s) 170, 289
Bst4CI ACNGT 3 cut(s) 205, 232, 244
Bst6I CTCTTC 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 101, 118
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 2 cut(s) 178, 423
BstKTI GATC 3 cut(s) 46, 157, 210
BstMBI GATC 3 cut(s) 43, 154, 207
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 2 cut(s) 170, 289
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 168, 287
BstSFI CTRYAG 2 cut(s) 17, 101
BstV1I GCAGC 2 cut(s) 3, 122
BstV2I GAAGAC 1 cut(s) 12
BstZ17I GTATAC 1 cut(s) 235
BsuRI GGCC 4 cut(s) 83, 99, 224, 422
BtsCI GGATG 2 cut(s) 178, 423
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 101, 118
CciI TCATGA 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 222, 438
CviAII CATG 4 cut(s) 92, 109, 185, 340
DdeI CTNAG 1 cut(s) 324
DpnI GATC 3 cut(s) 45, 156, 209
DpnII GATC 3 cut(s) 43, 154, 207
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 438
EcoRII CCWGG 2 cut(s) 168, 287
FaeI CATG 4 cut(s) 95, 112, 188, 343
FaiI YATR 9 cut(s) 93, 110, 186, 235, 280, 341, 353, 355, 427
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 385, 417
FaqI GGGAC 1 cut(s) 350
FatI CATG 4 cut(s) 91, 108, 184, 339
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 353
FblI GTMKAC 2 cut(s) 50, 234
Fnu4HI GCNGC 3 cut(s) 17, 136, 225
FokI GGATG 2 cut(s) 185, 430
Fsp4HI GCNGC 3 cut(s) 17, 136, 225
FspBI CTAG 3 cut(s) 77, 300, 459
GluI GCNGC 3 cut(s) 17, 136, 225
GsuI CTGGAG 2 cut(s) 271, 338
HaeIII GGCC 4 cut(s) 83, 99, 224, 422
HapII CCGG 1 cut(s) 152
Hin1II CATG 4 cut(s) 95, 112, 188, 343
HincII GTYRAC 2 cut(s) 51, 265
HindII GTYRAC 2 cut(s) 51, 265
HpaII CCGG 1 cut(s) 152
HphI GGTGA 3 cut(s) 260, 262, 382
Hpy166II GTNNAC 3 cut(s) 51, 235, 265
Hpy188I TCNGA 2 cut(s) 166, 191
Hpy188III TCNNGA 4 cut(s) 41, 109, 152, 295
Hpy8I GTNNAC 3 cut(s) 51, 235, 265
Hpy99I CGWCG 1 cut(s) 215
HpyCH4III ACNGT 3 cut(s) 205, 232, 244
HpyCH4V TGCA 3 cut(s) 19, 103, 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 4 cut(s) 95, 112, 188, 343
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 3 cut(s) 43, 154, 207
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 2 cut(s) 3, 122
MaeI CTAG 3 cut(s) 77, 300, 459
MaeIII GTNAC 2 cut(s) 370, 454
MalI GATC 3 cut(s) 45, 156, 209
MboI GATC 3 cut(s) 43, 154, 207
MboII GAAGA 3 cut(s) 17, 121, 317
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 6 cut(s) 73, 278, 301, 313, 429, 433
MroI TCCGGA 1 cut(s) 151
MseI TTAA 1 cut(s) 86
MslI CAYNNNNRTG 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 170, 289
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 2 cut(s) 170, 289
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeI CATATG 1 cut(s) 353
NdeII GATC 3 cut(s) 43, 154, 207
NlaIII CATG 4 cut(s) 95, 112, 188, 343
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 370, 454
NspI RCATGY 1 cut(s) 188
PagI TCATGA 1 cut(s) 108
PceI AGGCCT 1 cut(s) 83
PctI GAATGC 1 cut(s) 118
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 3 cut(s) 18, 137, 226
Psp6I CCWGG 2 cut(s) 168, 287
PspGI CCWGG 2 cut(s) 168, 287
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 2 cut(s) 222, 438
PstI CTGCAG 2 cut(s) 21, 105
RseI CAYNNNNRTG 1 cut(s) 356
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 3 cut(s) 17, 136, 225
Sau3AI GATC 3 cut(s) 43, 154, 207
Sau96I GGNCC 2 cut(s) 222, 438
SbfI CCTGCAGG 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 170, 289
SdaI CCTGCAGG 1 cut(s) 105
SfcI CTRYAG 2 cut(s) 17, 101
SinI GGWCC 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 450
SmoI CTYRAG 1 cut(s) 450
SpeI ACTAGT 1 cut(s) 458
Sse8387I CCTGCAGG 1 cut(s) 105
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 3 cut(s) 118, 133, 225
SspMI CTAG 3 cut(s) 77, 300, 459
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 168, 287
TaaI ACNGT 3 cut(s) 205, 232, 244
TaqI TCGA 2 cut(s) 50, 210
TauI GCSGC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 2 cut(s) 370, 454
TseI GCWGC 2 cut(s) 16, 135
Tsp45I GTSAC 2 cut(s) 370, 454
TspDTI ATGAA 3 cut(s) 125, 133, 267
TspGWI ACGGA 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 438
XceI RCATGY 1 cut(s) 188
XmiI GTMKAC 2 cut(s) 50, 234
XspI CTAG 3 cut(s) 77, 300, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.