Rh2DG425400
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015145
Physical Location & Seq
Forward (+)
61645442 .. 61648353
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG425400.1

Sequence Viewer

Length: 579 bp
ATGGAAGACGAGAAGCTGCAGAAGTCCAAGAAATGGCTTCACGATCTGTCGACAGGTGCCAAAGGTATAAACGAGCTAGAGGCCTTAACTCATGTTGGCCTGCAGGTCATGAATGCCCGCAAGGGCTTCTTCCGCTGCAACTTCATCGTTCCAGACCATCTCTCTGACCAGGATGGAAACTGGCATGTCGGAGCTATCGCAACTGTGATCGACGATGTTGGGGCCGCCACCGTATACTCAACTGTTGGTGATGTCAAATCCGTTGACTTCACCATTTCATATTACTCCAGGGTCAAGACTAGAGTTCAGTCTCTCTCTCTCTCTATCTTAACTCAACTTTTTGAGTCAGACCCATATCACCAGTCTTTGAAACCTTTGTGCTTGAGATTGATATTTGAATCTCTGATGCAGGAAGAGGTTGAGTTGGAGGCTGAGATTGTAGGGGACATGGGGAAGCTCATATGTGTGGTGGTAAAGGTGACAAGGAAAGAGGATGGAGAAAGCGTTGCTTTGGGTAAGCAATGGATGGCCTCATTTTCAAGGAGGACCAATCAATCTCAAGTCACTAGTAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

192

Amino Acids

21.52

Weight (kDa)

5.96

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52191 AT3G16175
fragaria_vesca FvH4_6g31870 FvH4_6g31870
rosa_chinensis RchiOBHm_Chr2g0139201
rosa_laevigata RLG00000019794
rosa_multiflora Rmu_sc0001803.1_g000007 Rmu_sc0037230.1_g000003
rosa_roxburghii Rroxscaffold_2G00105560
rosa_rugosa Rorug02G0355500 Rorug02G0355500
rosa_samantha Rh2AG405700 Rh2BG416200 Rh2CG391700 Rh2DG425400
rosa_wichuraiana Rw2G033130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 33
AccI GTMKAC 2 cut(s) 50, 234
AciI CCGC 3 cut(s) 118, 133, 225
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 3 cut(s) 370, 398, 540
AhlI ACTAGT 1 cut(s) 566
AjnI CCWGG 2 cut(s) 168, 287
AluBI AGCT 4 cut(s) 16, 76, 194, 457
AluI AGCT 4 cut(s) 16, 76, 194, 457
Alw26I GTCTC 1 cut(s) 315
AoxI GGCC 4 cut(s) 81, 97, 222, 528
ApeKI GCWGC 2 cut(s) 16, 135
AspS9I GGNCC 2 cut(s) 222, 546
AsuHPI GGTGA 4 cut(s) 260, 262, 350, 490
AvaII GGWCC 1 cut(s) 546
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 3, 122
BccI CCATC 4 cut(s) 165, 167, 488, 520
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 2 cut(s) 170, 289
BcoDI GTCTC 1 cut(s) 315
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 3 cut(s) 77, 300, 567
BfmI CTRYAG 2 cut(s) 17, 101
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 3 cut(s) 17, 136, 225
BlsI GCNGC 3 cut(s) 18, 137, 226
Bme1390I CCNGG 2 cut(s) 170, 289
Bme18I GGWCC 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 222, 546
BmiI GGNNCC 2 cut(s) 58, 223
BmrFI CCNGG 2 cut(s) 170, 289
BmsI GCATC 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 271
BpuEI CTTGAG 2 cut(s) 403, 543
BsaJI CCNNGG 1 cut(s) 288
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 185, 361
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 2 cut(s) 170, 289
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 3 cut(s) 178, 499, 531
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 2 cut(s) 185, 361
BseXI GCAGC 2 cut(s) 3, 122
BshFI GGCC 4 cut(s) 83, 99, 224, 530
BshNI GGYRCC 1 cut(s) 56
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 4 cut(s) 83, 99, 224, 530
Bsp143I GATC 2 cut(s) 43, 207
BspACI CCGC 3 cut(s) 118, 133, 225
BspANI GGCC 4 cut(s) 83, 99, 224, 530
BspCNI CTCAG 1 cut(s) 424
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 58, 223
BspMAI CTGCAG 2 cut(s) 21, 105
BspMI ACCTGC 1 cut(s) 94
BspT107I GGYRCC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 527
BsrI ACTGG 2 cut(s) 185, 361
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 2 cut(s) 43, 207
BssNAI GTATAC 1 cut(s) 235
Bst1107I GTATAC 1 cut(s) 235
Bst2UI CCWGG 2 cut(s) 170, 289
Bst4CI ACNGT 3 cut(s) 205, 232, 244
Bst6I CTCTTC 1 cut(s) 408
BstC8I GCNNGC 2 cut(s) 101, 118
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 3 cut(s) 178, 499, 531
BstKTI GATC 2 cut(s) 46, 210
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 2 cut(s) 43, 207
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 2 cut(s) 170, 289
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 168, 287
BstSFI CTRYAG 2 cut(s) 17, 101
BstV1I GCAGC 2 cut(s) 3, 122
BstV2I GAAGAC 1 cut(s) 12
BstZ17I GTATAC 1 cut(s) 235
BsuRI GGCC 4 cut(s) 83, 99, 224, 530
BtsCI GGATG 3 cut(s) 178, 499, 531
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 101, 118
CciI TCATGA 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 222, 546
CviAII CATG 4 cut(s) 92, 109, 185, 448
DdeI CTNAG 1 cut(s) 432
DpnI GATC 2 cut(s) 45, 209
DpnII GATC 2 cut(s) 43, 207
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco147I AGGCCT 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 546
EcoRII CCWGG 2 cut(s) 168, 287
FaeI CATG 4 cut(s) 95, 112, 188, 451
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 493, 525
FaqI GGGAC 1 cut(s) 458
FatI CATG 4 cut(s) 91, 108, 184, 447
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 461
FblI GTMKAC 2 cut(s) 50, 234
Fnu4HI GCNGC 3 cut(s) 17, 136, 225
FokI GGATG 3 cut(s) 185, 506, 538
Fsp4HI GCNGC 3 cut(s) 17, 136, 225
FspBI CTAG 3 cut(s) 77, 300, 567
GluI GCNGC 3 cut(s) 17, 136, 225
GsuI CTGGAG 1 cut(s) 271
HaeIII GGCC 4 cut(s) 83, 99, 224, 530
Hin1II CATG 4 cut(s) 95, 112, 188, 451
HincII GTYRAC 2 cut(s) 51, 265
HindII GTYRAC 2 cut(s) 51, 265
HinfI GANTC 2 cut(s) 344, 398
HphI GGTGA 4 cut(s) 260, 262, 350, 490
Hpy166II GTNNAC 3 cut(s) 51, 235, 265
Hpy188I TCNGA 4 cut(s) 166, 191, 349, 405
Hpy188III TCNNGA 4 cut(s) 41, 109, 152, 295
Hpy8I GTNNAC 3 cut(s) 51, 235, 265
Hpy99I CGWCG 1 cut(s) 215
HpyCH4III ACNGT 3 cut(s) 205, 232, 244
HpyCH4V TGCA 4 cut(s) 19, 103, 138, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 432
Hsp92II CATG 4 cut(s) 95, 112, 188, 451
Kzo9I GATC 2 cut(s) 43, 207
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 2 cut(s) 3, 122
LweI GCATC 1 cut(s) 396
MaeI CTAG 3 cut(s) 77, 300, 567
MaeIII GTNAC 2 cut(s) 478, 562
MalI GATC 2 cut(s) 45, 209
MboI GATC 2 cut(s) 43, 207
MboII GAAGA 3 cut(s) 17, 121, 425
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 2 cut(s) 169, 405
MnlI CCTC 6 cut(s) 73, 409, 421, 484, 537, 541
MseI TTAA 2 cut(s) 86, 329
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 135
MspR9I CCNGG 2 cut(s) 170, 289
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 2 cut(s) 170, 289
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeI CATATG 1 cut(s) 461
NdeII GATC 2 cut(s) 43, 207
NlaIII CATG 4 cut(s) 95, 112, 188, 451
NlaIV GGNNCC 2 cut(s) 58, 223
NmuCI GTSAC 2 cut(s) 478, 562
NspI RCATGY 1 cut(s) 188
PagI TCATGA 1 cut(s) 108
PceI AGGCCT 1 cut(s) 83
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 398
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 3 cut(s) 18, 137, 226
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 168, 287
PspGI CCWGG 2 cut(s) 168, 287
PspN4I GGNNCC 2 cut(s) 58, 223
PspPI GGNCC 2 cut(s) 222, 546
PstI CTGCAG 2 cut(s) 21, 105
RseI CAYNNNNRTG 1 cut(s) 464
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 86, 329
SatI GCNGC 3 cut(s) 17, 136, 225
Sau3AI GATC 2 cut(s) 43, 207
Sau96I GGNCC 2 cut(s) 222, 546
SbfI CCTGCAGG 1 cut(s) 105
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 2 cut(s) 170, 289
SdaI CCTGCAGG 1 cut(s) 105
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 2 cut(s) 17, 101
SinI GGWCC 1 cut(s) 546
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 2 cut(s) 382, 558
SmoI CTYRAG 2 cut(s) 382, 558
SpeI ACTAGT 1 cut(s) 566
Sse8387I CCTGCAGG 1 cut(s) 105
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 3 cut(s) 118, 133, 225
SspMI CTAG 3 cut(s) 77, 300, 567
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 168, 287
TaaI ACNGT 3 cut(s) 205, 232, 244
TaqI TCGA 2 cut(s) 50, 210
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 2 cut(s) 86, 329
Tru9I TTAA 2 cut(s) 86, 329
TseFI GTSAC 2 cut(s) 478, 562
TseI GCWGC 2 cut(s) 16, 135
Tsp45I GTSAC 2 cut(s) 478, 562
TspDTI ATGAA 3 cut(s) 125, 133, 267
TspGWI ACGGA 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 546
XceI RCATGY 1 cut(s) 188
XmiI GTMKAC 2 cut(s) 50, 234
XspI CTAG 3 cut(s) 77, 300, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.