Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0016893

Physical Location & Seq

Genomic Coordinates Forward (+)

32226389 .. 32227537

Gene Structure 1149 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00108240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGTGGAGCGACCTCCCCTTTGATCTTCTCTCCAACATCTTCTTCTTCCTCTCCCCCGACTCTTTTGCTTGCGCCAAGGCAGCATGTCGAAACTGGCATCAAGCTTGTGCCTCCACACCGAGCTTCAAGAACCCGTTTTTGTTGCAGAAGCAACTGCACCCTCCATGGTTCATAGCATTGCCTCTTCGAAACAACAGCCTTTCTTGCTATGCTCACAATCCAATCAACACCAACAACAACTGGTATGAAATTTCTTTAGATTTCTTACCCCACCCAATTCGTCTCGTGGCTCACTTAGAAGGCCTCCTCTTGGTCCGACCCTCCATCTCTACCGTTCTCCAATTGGGTGTCTGCAACCCATTTACAAGGCAATACAAGCAGCTTCCAACGTTAAACATCAGAAGAACCAACCCGGCCGTGGGGGTGATATCACTCCCAGCATTTTCAAACACTAAATTAATGTTCGGAACAGATTCACTTCATTGCTTCGGAATCTACGTGGCTGGTGGGATGTCGGAGGCGCCCCGCGGCGGTGCCACGTACGAGCCGACGCTAGAAATGTACGACCCTCAGCTCGACGCGTGGCGAGTTGTCGGGTCGATGCCGATGGAGTTTGCAGTGAGGCTAACAGTGTGGACTCCAAAGGAGAGTGTGTACTGTGATGGGGTGCTGTATTGGATGACCTCTGCCAGGGCATACAGCTTAATGGGGTGTGACATTTCTTCTAACAGTTGGAGAGAGCTGAGAGTGCCAATGGCGGACAAGCTGGAGTTTGCTGTGCTGGTTCTGCGCAATGGGAGGCTGACACTTGTGGGAGGGACATGTGCTTTGGGGGTTTGTATATGGGAGCTTGGTGAGGGAGAGAGTTGGGTGATGGTTGAGACGGTTCCTAGTGAATTGGGGATGAGGTTTATAGGGGAGAAGGGAAGTTGGGATAGAACAAAGTGTGTGGGGAGTGATGGGGCTATTTACTTGTATAGGGACCTTTGGGCAGGAATGGTGGTTTGGAGGGAAGTTGGAGAAGGGAATAGTAAGTGGGAATGGTTTTGGATTGAAGGGTGTTCGTCGATTAGAGGTAAACAAGTGAGAAATTTGCAAATAAAGGGAGTTCTTATGCATCCTAATCTTGTACCCTCATCCATTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009987 GO:0010468 GO:0010498 GO:0010556 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031146 GO:0031323 GO:0031326 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051603 GO:0060255 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like Kelch_1

Protein Analysis

382

Amino Acids

43.04

Weight (kDa)

7.51

Isoelectric Point (pI)

41.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box-like	PF12937	2 - 39	3.5e-07	F-box-like
F-box	PF00646	2 - 42	9.2e-07	F-box domain
FBA_1	PF07734	88 - 272	1.3e-06	F-box associated beta propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016893)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30250
malus_domestica	MD17G1206200.v1.1
prunus_persica	Prupe.3G081200_v2.0.a1
pyrus_communis	pycom17g20940
rosa_chinensis	RchiOBHm_Chr2g0136491
rosa_laevigata	RLG00000019536
rosa_multiflora	Rmu_sc0015604.1_g000002
rosa_roxburghii	Rroxscaffold_2G00108240
rosa_rugosa	Rorug02G0331300
rosa_samantha	Rh2AG382100 Rh2BG388700 Rh2CG368400 Rh2DG405400
rosa_wichuraiana	Rw2G031230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	791
AccB1I	GGYRCC	2 cut(s)	520, 533
AccII	CGCG	2 cut(s)	528, 581
AciI	CCGC	4 cut(s)	526, 528, 531, 758
AclI	AACGTT	1 cut(s)	389
AcoI	YGGCCR	1 cut(s)	414
AcsI	RAATTY	2 cut(s)	249, 1090
AcyI	GRCGYC	1 cut(s)	521
AfaI	GTAC	4 cut(s)	542, 563, 656, 1131
AfiI	CCNNNNNNNGG	5 cut(s)	310, 418, 419, 530, 690
AflIII	ACRYGT	2 cut(s)	579, 821
AgsI	TTSAA	3 cut(s)	127, 447, 1055
AjnI	CCWGG	1 cut(s)	689
AjuI	GAANNNNNNNTTGG	4 cut(s)	26, 58, 988, 1020
AluBI	AGCT	8 cut(s)	104, 123, 382, 574, 702, 742, 766, 850
AluI	AGCT	8 cut(s)	104, 123, 382, 574, 702, 742, 766, 850
Alw26I	GTCTC	2 cut(s)	287, 875
AoxI	GGCC	2 cut(s)	301, 414
ApeKI	GCWGC	2 cut(s)	80, 379
ApoI	RAATTY	2 cut(s)	249, 1090
ArsI	GACNNNNNNTTYG	3 cut(s)	34, 811, 843
AseI	ATTAAT	1 cut(s)	458
Asp700I	GAANNNNTTC	1 cut(s)	472
AspLEI	GCGC	3 cut(s)	74, 523, 792
AspS9I	GGNCC	2 cut(s)	313, 982
AsuC2I	CCSGG	1 cut(s)	413
AsuHPI	GGTGA	3 cut(s)	436, 866, 883
AsuII	TTCGAA	1 cut(s)	187
AvaII	GGWCC	2 cut(s)	313, 982
BanI	GGYRCC	2 cut(s)	520, 533
BauI	CACGAG	1 cut(s)	284
BbvCI	CCTCAGC	1 cut(s)	570
BbvI	GCAGC	2 cut(s)	92, 391
BccI	CCATC	5 cut(s)	332, 601, 656, 868, 953
BceAI	ACGGC	1 cut(s)	401
BcgI	CGANNNNNNTGC	2 cut(s)	47, 81
BciT130I	CCWGG	1 cut(s)	691
BcnI	CCSGG	1 cut(s)	413
BcoDI	GTCTC	2 cut(s)	287, 875
BfaI	CTAG	2 cut(s)	554, 891
BfoI	RGCGCY	1 cut(s)	524
BisI	GCNGC	3 cut(s)	81, 380, 529
BlsI	GCNGC	3 cut(s)	82, 381, 530
Bme1390I	CCNGG	2 cut(s)	413, 691
Bme18I	GGWCC	2 cut(s)	313, 982
BmgT120I	GGNCC	2 cut(s)	313, 982
BmiI	GGNNCC	4 cut(s)	522, 535, 888, 983
BmrFI	CCNGG	2 cut(s)	413, 691
BmsI	GCATC	3 cut(s)	106, 591, 1126
BpmI	CTGGAG	1 cut(s)	788
Bpu10I	CCTNAGC	1 cut(s)	570
Bpu14I	TTCGAA	1 cut(s)	187
BpuMI	CCSGG	1 cut(s)	413
BsaAI	YACGTR	2 cut(s)	499, 540
BsaHI	GRCGYC	1 cut(s)	521
BsaJI	CCNNGG	5 cut(s)	75, 164, 417, 526, 690
BsaXI	ACNNNNNCTCC	2 cut(s)	638, 668
Bsc4I	CCNNNNNNNGG	5 cut(s)	310, 418, 419, 530, 690
Bse1I	ACTGG	2 cut(s)	98, 245
Bse3DI	GCAATG	3 cut(s)	176, 481, 799
BseBI	CCWGG	1 cut(s)	691
BseDI	CCNNGG	5 cut(s)	75, 164, 417, 526, 690
BseGI	GGATG	5 cut(s)	516, 684, 909, 1117, 1136
BseLI	CCNNNNNNNGG	5 cut(s)	310, 418, 419, 530, 690
BseMI	GCAATG	3 cut(s)	176, 481, 799
BseMII	CTCAG	2 cut(s)	584, 734
BseNI	ACTGG	2 cut(s)	98, 245
BseRI	GAGGAG	1 cut(s)	296
BseX3I	CGGCCG	1 cut(s)	414
BseXI	GCAGC	2 cut(s)	92, 391
BseYI	CCCAGC	1 cut(s)	436
BsgI	GTGCAG	1 cut(s)	140
Bsh1236I	CGCG	2 cut(s)	528, 581
Bsh1285I	CGRYCG	1 cut(s)	417
BshFI	GGCC	2 cut(s)	303, 416
BshNI	GGYRCC	2 cut(s)	520, 533
BsiEI	CGRYCG	1 cut(s)	417
BsiSI	CCGG	1 cut(s)	413
BsiWI	CGTACG	1 cut(s)	540
BslFI	GGGAC	2 cut(s)	832, 995
BslI	CCNNNNNNNGG	5 cut(s)	310, 418, 419, 530, 690
BsmAI	GTCTC	2 cut(s)	287, 875
BsmBI	CGTCTC	2 cut(s)	287, 875
BsmFI	GGGAC	2 cut(s)	832, 995
BsnI	GGCC	2 cut(s)	303, 416
Bsp119I	TTCGAA	1 cut(s)	187
Bsp143I	GATC	1 cut(s)	22
Bsp19I	CCATGG	1 cut(s)	164
BspACI	CCGC	4 cut(s)	526, 528, 531, 758
BspANI	GGCC	2 cut(s)	303, 416
BspCNI	CTCAG	2 cut(s)	583, 735
BspFNI	CGCG	2 cut(s)	528, 581
BspLI	GGNNCC	4 cut(s)	522, 535, 888, 983
BspT104I	TTCGAA	1 cut(s)	187
BspT107I	GGYRCC	2 cut(s)	520, 533
BsrDI	GCAATG	3 cut(s)	176, 481, 799
BsrI	ACTGG	2 cut(s)	98, 245
BssECI	CCNNGG	5 cut(s)	75, 164, 417, 526, 690
BssMI	GATC	1 cut(s)	22
BssNI	GRCGYC	1 cut(s)	521
BssSI	CACGAG	1 cut(s)	284
BssT1I	CCWWGG	2 cut(s)	75, 164
Bst2BI	CACGAG	1 cut(s)	284
Bst2UI	CCWGG	1 cut(s)	691
Bst4CI	ACNGT	5 cut(s)	334, 631, 659, 731, 886
Bst6I	CTCTTC	1 cut(s)	189
BstACI	GRCGYC	1 cut(s)	521
BstBAI	YACGTR	2 cut(s)	499, 540
BstBI	TTCGAA	1 cut(s)	187
BstC8I	GCNNGC	1 cut(s)	70
BstDEI	CTNAG	3 cut(s)	295, 570, 743
BstDSI	CCRYGG	3 cut(s)	164, 417, 526
BstENI	CCTNNNNNAGG	1 cut(s)	688
BstF5I	GGATG	5 cut(s)	516, 684, 909, 1117, 1136
BstFNI	CGCG	2 cut(s)	528, 581
BstH2I	RGCGCY	1 cut(s)	524
BstHHI	GCGC	3 cut(s)	74, 523, 792
BstKTI	GATC	1 cut(s)	25
BstMAI	GTCTC	2 cut(s)	287, 875
BstMBI	GATC	1 cut(s)	22
BstMCI	CGRYCG	1 cut(s)	417
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 204, 376, 748, 787
BstNI	CCWGG	1 cut(s)	691
BstNSI	RCATGY	2 cut(s)	87, 825
BstSCI	CCNGG	2 cut(s)	411, 689
BstUI	CGCG	2 cut(s)	528, 581
BstV1I	GCAGC	2 cut(s)	92, 391
BstZI	CGGCCG	1 cut(s)	414
BsuRI	GGCC	2 cut(s)	303, 416
BtgI	CCRYGG	3 cut(s)	164, 417, 526
BtsCI	GGATG	5 cut(s)	516, 684, 909, 1117, 1136
BtsI	GCAGTG	1 cut(s)	624
BtsIMutI	CAGTG	2 cut(s)	624, 636
Cac8I	GCNNGC	1 cut(s)	70
CfoI	GCGC	3 cut(s)	74, 523, 792
Cfr13I	GGNCC	2 cut(s)	313, 982
Cfr42I	CCGCGG	1 cut(s)	529
CseI	GACGC	2 cut(s)	559, 587
Csp6I	GTAC	4 cut(s)	541, 562, 655, 1130
CspCI	CAANNNNNGTGG	2 cut(s)	930, 965
CviAII	CATG	3 cut(s)	84, 165, 822
CviQI	GTAC	4 cut(s)	541, 562, 655, 1130
DdeI	CTNAG	3 cut(s)	295, 570, 743
DinI	GGCGCC	1 cut(s)	522
DpnI	GATC	1 cut(s)	24
DpnII	GATC	1 cut(s)	22
EaeI	YGGCCR	1 cut(s)	414
EagI	CGGCCG	1 cut(s)	414
Eam1104I	CTCTTC	1 cut(s)	189
EarI	CTCTTC	1 cut(s)	189
EciI	GGCGGA	1 cut(s)	773
EclXI	CGGCCG	1 cut(s)	414
Eco130I	CCWWGG	2 cut(s)	75, 164
Eco147I	AGGCCT	1 cut(s)	303
Eco32I	GATATC	1 cut(s)	429
Eco47I	GGWCC	2 cut(s)	313, 982
Eco52I	CGGCCG	1 cut(s)	414
EcoNI	CCTNNNNNAGG	1 cut(s)	688
EcoO109I	RGGNCCY	1 cut(s)	982
EcoRII	CCWGG	1 cut(s)	689
EcoRV	GATATC	1 cut(s)	429
EcoT14I	CCWWGG	2 cut(s)	75, 164
EcoT22I	ATGCAT	1 cut(s)	1119
EgeI	GGCGCC	1 cut(s)	522
EheI	GGCGCC	1 cut(s)	522
ErhI	CCWWGG	2 cut(s)	75, 164
Esp3I	CGTCTC	2 cut(s)	287, 875
FaeI	CATG	3 cut(s)	87, 168, 825
FaqI	GGGAC	2 cut(s)	832, 995
FatI	CATG	3 cut(s)	83, 164, 821
FauI	CCCGC	1 cut(s)	533
Fnu4HI	GCNGC	3 cut(s)	81, 380, 529
FokI	GGATG	5 cut(s)	523, 691, 916, 1104, 1123
Fsp4HI	GCNGC	3 cut(s)	81, 380, 529
FspBI	CTAG	2 cut(s)	554, 891
FspI	TGCGCA	1 cut(s)	791
GlaI	GCGC	3 cut(s)	73, 522, 791
GluI	GCNGC	3 cut(s)	81, 380, 529
GsaI	CCCAGC	1 cut(s)	440
GsuI	CTGGAG	1 cut(s)	788
HaeII	RGCGCY	1 cut(s)	524
HaeIII	GGCC	2 cut(s)	303, 416
HapII	CCGG	1 cut(s)	413
HgaI	GACGC	2 cut(s)	559, 587
HhaI	GCGC	3 cut(s)	74, 523, 792
Hin1I	GRCGYC	1 cut(s)	521
Hin1II	CATG	3 cut(s)	87, 168, 825
Hin6I	GCGC	3 cut(s)	72, 521, 790
HinP1I	GCGC	3 cut(s)	72, 521, 790
HindIII	AAGCTT	1 cut(s)	102
HinfI	GANTC	4 cut(s)	59, 473, 492, 637
HpaII	CCGG	1 cut(s)	413
HphI	GGTGA	3 cut(s)	436, 866, 883
Hpy166II	GTNNAC	3 cut(s)	636, 655, 1079
Hpy188I	TCNGA	5 cut(s)	317, 401, 467, 491, 517
Hpy188III	TCNNGA	1 cut(s)	127
Hpy8I	GTNNAC	3 cut(s)	636, 655, 1079
Hpy99I	CGWCG	3 cut(s)	553, 581, 1069
HpyAV	CCTTC	4 cut(s)	293, 916, 1016, 1049
HpyCH4III	ACNGT	5 cut(s)	334, 631, 659, 731, 886
HpyCH4IV	ACGT	3 cut(s)	389, 498, 539
HpyCH4V	TGCA	6 cut(s)	145, 157, 354, 617, 1096, 1117
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 204, 376, 748, 787
HpyF3I	CTNAG	3 cut(s)	295, 570, 743
HpySE526I	ACGT	3 cut(s)	389, 498, 539
Hsp92I	GRCGYC	1 cut(s)	521
Hsp92II	CATG	3 cut(s)	87, 168, 825
HspAI	GCGC	3 cut(s)	72, 521, 790
KasI	GGCGCC	1 cut(s)	520
KspI	CCGCGG	1 cut(s)	529
Kzo9I	GATC	1 cut(s)	22
LmnI	GCTCC	2 cut(s)	6, 847
Lsp1109I	GCAGC	2 cut(s)	92, 391
LweI	GCATC	3 cut(s)	106, 591, 1126
MaeI	CTAG	2 cut(s)	554, 891
MaeII	ACGT	3 cut(s)	389, 498, 539
MaeIII	GTNAC	1 cut(s)	713
MalI	GATC	1 cut(s)	24
MboI	GATC	1 cut(s)	22
MboII	GAAGA	7 cut(s)	17, 31, 34, 37, 176, 414, 714
MfeI	CAATTG	1 cut(s)	341
MluCI	AATT	6 cut(s)	249, 276, 341, 455, 896, 1090
MluI	ACGCGT	1 cut(s)	579
Mly113I	GGCGCC	1 cut(s)	521
MlyI	GAGTC	2 cut(s)	53, 631
MmeI	TCCRAC	6 cut(s)	57, 340, 410, 495, 713, 997
Mph1103I	ATGCAT	1 cut(s)	1119
MroXI	GAANNNNTTC	1 cut(s)	472
MseI	TTAA	3 cut(s)	392, 458, 704
MspA1I	CMGCKG	1 cut(s)	528
MspI	CCGG	1 cut(s)	413
MspR9I	CCNGG	2 cut(s)	413, 691
MunI	CAATTG	1 cut(s)	341
MvaI	CCWGG	1 cut(s)	691
MvnI	CGCG	2 cut(s)	528, 581
MwoI	GCNNNNNNNGC	5 cut(s)	80, 204, 376, 748, 787
NarI	GGCGCC	1 cut(s)	521
NciI	CCSGG	1 cut(s)	413
NcoI	CCATGG	1 cut(s)	164
NdeII	GATC	1 cut(s)	22
NlaIII	CATG	3 cut(s)	87, 168, 825
NlaIV	GGNNCC	4 cut(s)	522, 535, 888, 983
NmuCI	GTSAC	1 cut(s)	713
NsbI	TGCGCA	1 cut(s)	791
NsiI	ATGCAT	1 cut(s)	1119
NspI	RCATGY	2 cut(s)	87, 825
NspV	TTCGAA	1 cut(s)	187
PceI	AGGCCT	1 cut(s)	303
PciI	ACATGT	1 cut(s)	821
PcsI	WCGNNNNNNNCGW	2 cut(s)	495, 545
PdmI	GAANNNNTTC	1 cut(s)	472
PfeI	GAWTC	2 cut(s)	473, 492
Pfl23II	CGTACG	1 cut(s)	540
PkrI	GCNGC	3 cut(s)	82, 381, 530
PleI	GAGTC	2 cut(s)	53, 631
PluTI	GGCGCC	1 cut(s)	524
PpsI	GAGTC	2 cut(s)	53, 631
Ppu21I	YACGTR	2 cut(s)	499, 540
PpuMI	RGGWCCY	1 cut(s)	982
PscI	ACATGT	1 cut(s)	821
PshBI	ATTAAT	1 cut(s)	458
Psp1406I	AACGTT	1 cut(s)	389
Psp5II	RGGWCCY	1 cut(s)	982
Psp6I	CCWGG	1 cut(s)	689
PspFI	CCCAGC	1 cut(s)	436
PspGI	CCWGG	1 cut(s)	689
PspLI	CGTACG	1 cut(s)	540
PspN4I	GGNNCC	4 cut(s)	522, 535, 888, 983
PspPI	GGNCC	2 cut(s)	313, 982
PspPPI	RGGWCCY	1 cut(s)	982
RsaI	GTAC	4 cut(s)	542, 563, 656, 1131
RsaNI	GTAC	4 cut(s)	541, 562, 655, 1130
SacII	CCGCGG	1 cut(s)	529
SaqAI	TTAA	3 cut(s)	392, 458, 704
SatI	GCNGC	3 cut(s)	81, 380, 529
Sau3AI	GATC	1 cut(s)	22
Sau96I	GGNCC	2 cut(s)	313, 982
SchI	GAGTC	2 cut(s)	53, 631
ScrFI	CCNGG	2 cut(s)	413, 691
SfaNI	GCATC	3 cut(s)	106, 591, 1126
SfoI	GGCGCC	1 cut(s)	522
Sfr303I	CCGCGG	1 cut(s)	529
SfuI	TTCGAA	1 cut(s)	187
SgrBI	CCGCGG	1 cut(s)	529
SinI	GGWCC	2 cut(s)	313, 982
Sse9I	AATT	6 cut(s)	249, 276, 341, 455, 896, 1090
SseBI	AGGCCT	1 cut(s)	303
SsiI	CCGC	4 cut(s)	526, 528, 531, 758
SspDI	GGCGCC	1 cut(s)	520
SspMI	CTAG	2 cut(s)	554, 891
StuI	AGGCCT	1 cut(s)	303
StyD4I	CCNGG	2 cut(s)	411, 689
StyI	CCWWGG	2 cut(s)	75, 164
TaaI	ACNGT	5 cut(s)	334, 631, 659, 731, 886
TaiI	ACGT	3 cut(s)	392, 501, 542
TaqI	TCGA	5 cut(s)	88, 187, 576, 599, 1067
TasI	AATT	6 cut(s)	249, 276, 341, 455, 896, 1090
TatI	WGTACW	1 cut(s)	654
TauI	GCSGC	1 cut(s)	531
TfiI	GAWTC	2 cut(s)	473, 492
Tru1I	TTAA	3 cut(s)	392, 458, 704
Tru9I	TTAA	3 cut(s)	392, 458, 704
TscAI	CASTG	2 cut(s)	624, 636
TseFI	GTSAC	1 cut(s)	713
TseI	GCWGC	2 cut(s)	80, 379
Tsp45I	GTSAC	1 cut(s)	713
TspDTI	ATGAA	3 cut(s)	160, 261, 470
TspRI	CASTG	2 cut(s)	624, 636
VpaK11BI	GGWCC	2 cut(s)	313, 982
VspI	ATTAAT	1 cut(s)	458
XagI	CCTNNNNNAGG	1 cut(s)	688
XapI	RAATTY	2 cut(s)	249, 1090
XceI	RCATGY	2 cut(s)	87, 825
XcmI	CCANNNNNNNNNTGG	1 cut(s)	415
XmnI	GAANNNNTTC	1 cut(s)	472
XspI	CTAG	2 cut(s)	554, 891
Zsp2I	ATGCAT	1 cut(s)	1119

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.