Rroxscaffold_3G00231510

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0019714
Physical Location & Seq
Reverse (-)
16778732 .. 16793588
14857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231510.1

Sequence Viewer

Length: 621 bp
ATGGCAAAGGCAAAAGACAGTCTGAAGGGCAGATGCGTAGCCAAATCAATTCATACTCAAATTCAAAGAAAATTTGGAGTTGCAATCTACCTTCGAGAGGAATGGAGAATGATTGAAGGTCACGGCATTTCTTTTCCTGGAAATAAGATTCCTTCGTGGTGTTCTCCTCATGTCAATTATAGAGGTCGCACAGTCACTTTTGAAATGCCATTTGGAACTGCTATAGTGTTTTGTGTGTGCATTGTTTATTCTCGCTCTCCAGGCGAGCATGACACTGATGATTATGAGTATGTGGTTGTAAATCATACTAGGCGCACAGGTTTTTTGGTCCACCAAGTGGATTTTCACACAACAAATTCCAATGAAAAGTATCTTTTTCTACGCAATATGTTGCTGAAGGATGCATTAAATTTGGAAGGTGGTGACTCTATAAGCCTTCGAGTATTACTGGGAGATCATTTTAAGTTGCTAATCCAACAACTGCAATCTGAGAATAGGAAGTTGCGAAGACAGTTGATACCATCACAGCAGCCAAATCAGATTGTTGTTAAAAGAAATTTTTTGGAACGGTTTCTTCCGGCAGAAGCTCAGATGACGACACAAGGACCTGACGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

206

Amino Acids

23.81

Weight (kDa)

9.23

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019714)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g32051
rosa_roxburghii Rroxscaffold_3G00231500 Rroxscaffold_3G00231510
rosa_samantha Rh7AG399700 Rh7CG417900 Rh7DG395700 Rh7DG395800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AcsI RAATTY 5 cut(s) 60, 71, 355, 409, 556
AcuI CTGAAG 2 cut(s) 44, 416
AdeI CACNNNGTG 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 97, 337
AgsI TTSAA 3 cut(s) 65, 116, 203
AjnI CCWGG 2 cut(s) 136, 259
AjuI GAANNNNNNNTTGG 4 cut(s) 195, 227, 327, 359
AluBI AGCT 1 cut(s) 587
AluI AGCT 1 cut(s) 587
ApeKI GCWGC 1 cut(s) 529
ApoI RAATTY 5 cut(s) 60, 71, 355, 409, 556
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 2 cut(s) 328, 605
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 2 cut(s) 328, 605
BbsI GAAGAC 1 cut(s) 514
BbvI GCAGC 1 cut(s) 541
BccI CCATC 1 cut(s) 529
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 138, 261
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 1 cut(s) 530
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 2 cut(s) 138, 261
Bme18I GGWCC 2 cut(s) 328, 605
BmgT120I GGNCC 2 cut(s) 328, 605
BmrFI CCNGG 2 cut(s) 138, 261
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 23, 391
BmuI ACTGGG 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 514
BpmI CTGGAG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 337
Bse1I ACTGG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 138, 261
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 2 cut(s) 97, 337
BseMII CTCAG 2 cut(s) 480, 602
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 541
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 2 cut(s) 97, 337
Bsp143I GATC 1 cut(s) 454
BspCNI CTCAG 2 cut(s) 481, 601
BsrI ACTGG 1 cut(s) 453
BssMI GATC 1 cut(s) 454
Bst2UI CCWGG 2 cut(s) 138, 261
Bst4CI ACNGT 4 cut(s) 20, 193, 513, 570
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 2 cut(s) 489, 588
BstENI CCTNNNNNAGG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 1 cut(s) 457
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 2 cut(s) 138, 261
BstSCI CCNGG 2 cut(s) 136, 259
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 1 cut(s) 514
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 2 cut(s) 328, 605
CviAII CATG 2 cut(s) 170, 269
CviJI RGCY 4 cut(s) 41, 435, 532, 587
CviKI_1 RGCY 4 cut(s) 41, 435, 532, 587
DdeI CTNAG 2 cut(s) 489, 588
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
DraIII CACNNNGTG 1 cut(s) 337
Eco47I GGWCC 2 cut(s) 328, 605
Eco57I CTGAAG 2 cut(s) 44, 416
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 605
EcoRII CCWGG 2 cut(s) 136, 259
EcoT22I ATGCAT 1 cut(s) 406
FaeI CATG 2 cut(s) 173, 272
FatI CATG 2 cut(s) 169, 268
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 1 cut(s) 413
Fsp4HI GCNGC 1 cut(s) 530
FspBI CTAG 1 cut(s) 309
GlaI GCGC 1 cut(s) 314
GluI GCNGC 1 cut(s) 530
GsuI CTGGAG 1 cut(s) 243
HapII CCGG 1 cut(s) 578
HhaI GCGC 1 cut(s) 315
Hin1II CATG 2 cut(s) 173, 272
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HinfI GANTC 2 cut(s) 148, 425
HpaII CCGG 1 cut(s) 578
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 4 cut(s) 24, 490, 540, 591
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 7 cut(s) 19, 101, 110, 162, 391, 410, 446
HpyCH4III ACNGT 4 cut(s) 20, 193, 513, 570
HpyCH4V TGCA 4 cut(s) 83, 240, 404, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 2 cut(s) 489, 588
Hsp92II CATG 2 cut(s) 173, 272
HspAI GCGC 1 cut(s) 313
Kzo9I GATC 1 cut(s) 454
LpnPI CCDG 7 cut(s) 123, 150, 246, 273, 303, 434, 591
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 2 cut(s) 23, 391
MaeI CTAG 1 cut(s) 309
MaeIII GTNAC 3 cut(s) 119, 193, 422
MalI GATC 1 cut(s) 456
MboI GATC 1 cut(s) 454
MboII GAAGA 2 cut(s) 519, 566
MluCI AATT 7 cut(s) 48, 60, 71, 175, 355, 409, 556
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 3 cut(s) 91, 176, 177
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 3 cut(s) 407, 462, 549
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 2 cut(s) 138, 261
MvaI CCWGG 2 cut(s) 138, 261
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 1 cut(s) 454
NlaIII CATG 2 cut(s) 173, 272
NmuCI GTSAC 3 cut(s) 119, 193, 422
NsiI ATGCAT 1 cut(s) 406
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 1 cut(s) 531
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 605
Psp6I CCWGG 2 cut(s) 136, 259
PspGI CCWGG 2 cut(s) 136, 259
PspPI GGNCC 2 cut(s) 328, 605
PspPPI RGGWCCY 1 cut(s) 605
SaqAI TTAA 3 cut(s) 407, 462, 549
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 2 cut(s) 328, 605
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 138, 261
SetI ASST 7 cut(s) 93, 121, 187, 322, 421, 589, 610
SfaNI GCATC 2 cut(s) 23, 391
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 2 cut(s) 328, 605
Sse9I AATT 7 cut(s) 48, 60, 71, 175, 355, 409, 556
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 136, 259
TaaI ACNGT 4 cut(s) 20, 193, 513, 570
TaqI TCGA 2 cut(s) 94, 439
TasI AATT 7 cut(s) 48, 60, 71, 175, 355, 409, 556
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 3 cut(s) 407, 462, 549
Tru9I TTAA 3 cut(s) 407, 462, 549
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 3 cut(s) 119, 193, 422
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 3 cut(s) 119, 193, 422
TspDTI ATGAA 2 cut(s) 41, 378
TspRI CASTG 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 328, 605
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 5 cut(s) 60, 71, 355, 409, 556
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.