Rroxscaffold_4G00279320

G-patch domain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0020072
Physical Location & Seq
Forward (+)
2135545 .. 2138577
3033 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00279320.1

Sequence Viewer

Length: 624 bp
ATGGCATGGCTGTTTGACGAAAGTCCCCGGTCAATTGTTCCTGGGTTTTATGGGTTTCGATTGTTGGGAACCTTTAGGGGGCAGCATGGCCCTGCACTTTGGAATGGTGCATTTTTCACTCATCAGCTCAGAGAGCCTTCATCTTCAAAGAGGCCCAAAAGAGAGTCCTCAGCTGCATTACCGGAGTCGAATGTCAAGAGACCTCGAGTTGCGTCAGCTGCATCATCATCGGCTCCCATCTCCAGAGCTAATGGTAAGGGGAAAGAGAGAGCCAATGAAGGCCTCAAACCTTGCCTTCCGGGGTTCAAGAGGTTTTCTTTGTCAGATCCTGATGTACCTGCTGCTCAGAATGTCAGCAACATGGCATCTAACCCAATCAACATACCAAATGTGGAGCAACCTGTCATTAATGAGGCTTTAGAAACTCCGTTTGGGTTTGCAAAAGGCAGCACTTTTACTATGAAGCTTTTTAAGAAGATGGGATACGAAGAAGGGGGTGGTTTGGGAAAAGATGGCCAAGGCACCAAGGAGCCTATTCGAGTTGGCATATGGACAGGAAAGCAGGGATTGGGCTTCAAAACAGCTCAGACATCAGAGCAGAATGCTGGGGATCAGAACCAGTAA

Protein Analysis

207

Amino Acids

22.34

Weight (kDa)

9.85

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 151 - 191 9.7e-11 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020072)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0006267.1_g000005
rosa_roxburghii Rroxscaffold_4G00279320
rosa_samantha Rh1AG446000 Rh1BG401300 Rh1CG415700 Rh1DG431500
rosa_wichuraiana Rw1G038820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AccB1I GGYRCC 1 cut(s) 521
AclWI GGATC 2 cut(s) 320, 618
AcoI YGGCCR 1 cut(s) 514
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 3 cut(s) 147, 307, 577
AjnI CCWGG 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 6 cut(s) 127, 173, 218, 248, 466, 584
AluI AGCT 6 cut(s) 127, 173, 218, 248, 466, 584
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 320, 618
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 4 cut(s) 88, 152, 280, 514
ApeKI GCWGC 5 cut(s) 82, 173, 218, 341, 447
AseI ATTAAT 1 cut(s) 408
AspS9I GGNCC 2 cut(s) 89, 153
AsuC2I CCSGG 2 cut(s) 28, 300
AvaI CYCGRG 1 cut(s) 204
BalI TGGCCA 1 cut(s) 516
BanI GGYRCC 1 cut(s) 521
BarI GAAGNNNNNNTAC 2 cut(s) 467, 499
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 5 cut(s) 94, 160, 205, 328, 459
BccI CCATC 3 cut(s) 245, 472, 506
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 1 cut(s) 42
BciVI GTATCC 1 cut(s) 476
BcnI CCSGG 2 cut(s) 28, 300
BcoDI GTCTC 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 346
BfuI GTATCC 1 cut(s) 476
BisI GCNGC 5 cut(s) 83, 174, 219, 342, 448
BlsI GCNGC 5 cut(s) 84, 175, 220, 343, 449
Bme1390I CCNGG 3 cut(s) 28, 42, 300
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 89, 153
BmiI GGNNCC 4 cut(s) 70, 234, 523, 531
BmrFI CCNGG 3 cut(s) 28, 42, 300
BmsI GCATC 2 cut(s) 230, 374
BoxI GACNNNNGTC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 226
Bpu10I CCTNAGC 1 cut(s) 169
BpuMI CCSGG 2 cut(s) 28, 300
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 5 cut(s) 26, 41, 299, 517, 525
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 1 cut(s) 619
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 5 cut(s) 26, 41, 299, 517, 525
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMII CTCAG 4 cut(s) 142, 183, 359, 599
BseNI ACTGG 1 cut(s) 619
BseXI GCAGC 5 cut(s) 94, 160, 205, 328, 459
BseYI CCCAGC 1 cut(s) 605
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 4 cut(s) 90, 154, 282, 516
BshNI GGYRCC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 3 cut(s) 28, 182, 299
BslFI GGGAC 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 9
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 4 cut(s) 90, 154, 282, 516
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 204
Bsp143I GATC 2 cut(s) 325, 610
BspANI GGCC 4 cut(s) 90, 154, 282, 516
BspCNI CTCAG 4 cut(s) 141, 182, 358, 598
BspLI GGNNCC 4 cut(s) 70, 234, 523, 531
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 2 cut(s) 320, 618
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 193
BsrI ACTGG 1 cut(s) 619
BssECI CCNNGG 5 cut(s) 26, 41, 299, 517, 525
BssMI GATC 2 cut(s) 325, 610
BssT1I CCWWGG 2 cut(s) 517, 525
Bst2UI CCWGG 1 cut(s) 42
BstDEI CTNAG 4 cut(s) 128, 169, 345, 585
BstKTI GATC 2 cut(s) 328, 613
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 2 cut(s) 325, 610
BstMWI GCNNNNNNNGC 2 cut(s) 133, 218
BstNI CCWGG 1 cut(s) 42
BstPAI GACNNNNGTC 1 cut(s) 21
BstSCI CCNGG 3 cut(s) 26, 40, 298
BstV1I GCAGC 5 cut(s) 94, 160, 205, 328, 459
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BsuI GTATCC 1 cut(s) 476
BsuRI GGCC 4 cut(s) 90, 154, 282, 516
BveI ACCTGC 1 cut(s) 346
CaiI CAGNNNCTG 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 89, 153
CseI GACGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 335
CviAII CATG 3 cut(s) 6, 86, 361
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 4 cut(s) 128, 169, 345, 585
DpnI GATC 2 cut(s) 327, 612
DpnII GATC 2 cut(s) 325, 610
EaeI YGGCCR 1 cut(s) 514
Eco130I CCWWGG 2 cut(s) 517, 525
Eco147I AGGCCT 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 517, 525
ErhI CCWWGG 2 cut(s) 517, 525
FaeI CATG 3 cut(s) 9, 89, 364
FaiI YATR 8 cut(s) 7, 51, 87, 362, 383, 461, 548, 550
FaqI GGGAC 1 cut(s) 9
FatI CATG 3 cut(s) 5, 85, 360
FauNDI CATATG 1 cut(s) 548
Fnu4HI GCNGC 5 cut(s) 83, 174, 219, 342, 448
Fsp4HI GCNGC 5 cut(s) 83, 174, 219, 342, 448
GluI GCNGC 5 cut(s) 83, 174, 219, 342, 448
GsaI CCCAGC 1 cut(s) 609
GsuI CTGGAG 1 cut(s) 226
HaeIII GGCC 4 cut(s) 90, 154, 282, 516
HapII CCGG 3 cut(s) 28, 182, 299
HgaI GACGC 1 cut(s) 201
Hin1II CATG 3 cut(s) 9, 89, 364
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 2 cut(s) 164, 185
HpaII CCGG 3 cut(s) 28, 182, 299
Hpy188I TCNGA 6 cut(s) 131, 325, 348, 588, 595, 615
Hpy188III TCNNGA 4 cut(s) 196, 243, 307, 329
HpyAV CCTTC 4 cut(s) 147, 272, 305, 485
HpyCH4V TGCA 5 cut(s) 95, 110, 176, 221, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 218
HpyF3I CTNAG 4 cut(s) 128, 169, 345, 585
Hsp92II CATG 3 cut(s) 9, 89, 364
Kzo9I GATC 2 cut(s) 325, 610
LmnI GCTCC 3 cut(s) 238, 394, 529
Lsp1109I GCAGC 5 cut(s) 94, 160, 205, 328, 459
LweI GCATC 2 cut(s) 230, 374
MalI GATC 2 cut(s) 327, 612
MboI GATC 2 cut(s) 325, 610
MboII GAAGA 3 cut(s) 135, 487, 500
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 516
MluCI AATT 1 cut(s) 33
MluNI TGGCCA 1 cut(s) 516
MlyI GAGTC 2 cut(s) 173, 194
MnlI CCTC 6 cut(s) 144, 178, 213, 293, 303, 406
Mox20I TGGCCA 1 cut(s) 516
MscI TGGCCA 1 cut(s) 516
MseI TTAA 2 cut(s) 408, 471
Msp20I TGGCCA 1 cut(s) 516
MspA1I CMGCKG 2 cut(s) 173, 218
MspI CCGG 3 cut(s) 28, 182, 299
MspR9I CCNGG 3 cut(s) 28, 42, 300
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 2 cut(s) 133, 218
NciI CCSGG 2 cut(s) 28, 300
NdeI CATATG 1 cut(s) 548
NdeII GATC 2 cut(s) 325, 610
NlaIII CATG 3 cut(s) 9, 89, 364
NlaIV GGNNCC 4 cut(s) 70, 234, 523, 531
PaeR7I CTCGAG 1 cut(s) 204
PceI AGGCCT 1 cut(s) 282
PctI GAATGC 1 cut(s) 607
PkrI GCNGC 5 cut(s) 84, 175, 220, 343, 449
PleI GAGTC 2 cut(s) 172, 193
PpsI GAGTC 2 cut(s) 172, 193
PshAI GACNNNNGTC 1 cut(s) 21
PshBI ATTAAT 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 4 cut(s) 70, 234, 523, 531
PspPI GGNCC 2 cut(s) 89, 153
PspXI VCTCGAGB 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 1 cut(s) 325
PvuII CAGCTG 2 cut(s) 173, 218
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SaqAI TTAA 2 cut(s) 408, 471
SatI GCNGC 5 cut(s) 83, 174, 219, 342, 448
Sau3AI GATC 2 cut(s) 325, 610
Sau96I GGNCC 2 cut(s) 89, 153
SchI GAGTC 2 cut(s) 173, 194
ScrFI CCNGG 3 cut(s) 28, 42, 300
SfaNI GCATC 2 cut(s) 230, 374
Sfr274I CTCGAG 1 cut(s) 204
SlaI CTCGAG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 1 cut(s) 33
SseBI AGGCCT 1 cut(s) 282
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 26, 40, 298
StyI CCWWGG 2 cut(s) 517, 525
TaqI TCGA 4 cut(s) 58, 188, 205, 538
TasI AATT 1 cut(s) 33
Tru1I TTAA 2 cut(s) 408, 471
Tru9I TTAA 2 cut(s) 408, 471
TseI GCWGC 5 cut(s) 82, 173, 218, 341, 447
TspDTI ATGAA 3 cut(s) 129, 291, 476
TspGWI ACGGA 1 cut(s) 417
VspI ATTAAT 1 cut(s) 408
XhoI CTCGAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.