Rh1DG431500

R3H domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0020072
Physical Location & Seq
Reverse (-)
64419606 .. 64421103
1498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG431500.1

Sequence Viewer

Length: 588 bp
ATGGGTTTCGATTGTTGGGAACCGTTTAGGGAGCAGCATGGCCCTGCACTTTGGAATGGTGCATTTTTCACTCATCAGCTCAGAGAGCCTTCATCTTCAAAGAGGCCCAAAAGAGAGTCCTCAGCTGCATTACCAGAGTCGAATGTCAAGAGACCCCGAGTTGTGTCAGCTGCATCATCATCGGCTCCCATCTCCAGAGCTAATGGTAAGGGGAAAGAGAGAGCCAATGAAGGCCTCAAACCTTGCCTTCCGGGGTTCAAGAGGTCTTCTTTGTCAGATCCTGATGTACCTACTGCTCAGAATGTCAGCAACATGGCATCTAACCCAATCAACATACCAAACGTGGAGCAACCTGTCACTAATGAGGCTTTAGAAACTTCGTTTGGGTTTGCAAAAAGTAGCACTTTTACTGTGGAGCTTTTTAAGAAGATGGGATACGAAGAAGGGAGTGGTTTGGGAAAAGATGGCCAAGGCACCAAGGAGCCTATTCGAGTTGGCATATGGACAAAAAAGCAGGGATTGGGCTTCAAAACAGCTCAGACATCAGAGCAGAATGCTGGGGATCAGAATGAGAAGCAGAACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-patch R3H

Protein Analysis

195

Amino Acids

21.21

Weight (kDa)

9.27

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 136 - 179 7.3e-06 G-patch domain
G-patch PF01585 138 - 175 1.6e-11 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020072)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0006267.1_g000005
rosa_roxburghii Rroxscaffold_4G00279320
rosa_samantha Rh1AG446000 Rh1BG401300 Rh1CG415700 Rh1DG431500
rosa_wichuraiana Rw1G038820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 473
AclWI GGATC 2 cut(s) 272, 570
AcoI YGGCCR 1 cut(s) 466
AfaI GTAC 1 cut(s) 288
AgsI TTSAA 3 cut(s) 99, 259, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 6 cut(s) 79, 125, 170, 200, 418, 536
AluI AGCT 6 cut(s) 79, 125, 170, 200, 418, 536
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 2 cut(s) 272, 570
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 4 cut(s) 40, 104, 232, 466
ApeKI GCWGC 3 cut(s) 34, 125, 170
AspS9I GGNCC 2 cut(s) 41, 105
AsuC2I CCSGG 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 156
BalI TGGCCA 1 cut(s) 468
BanI GGYRCC 1 cut(s) 473
BarI GAAGNNNNNNTAC 2 cut(s) 419, 451
BbsI GAAGAC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 121
BbvI GCAGC 3 cut(s) 46, 112, 157
BccI CCATC 3 cut(s) 197, 424, 458
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciVI GTATCC 1 cut(s) 428
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 145
BfuI GTATCC 1 cut(s) 428
BisI GCNGC 3 cut(s) 35, 126, 171
BlsI GCNGC 3 cut(s) 36, 127, 172
Bme1390I CCNGG 1 cut(s) 252
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 2 cut(s) 41, 105
BmiI GGNNCC 4 cut(s) 21, 186, 475, 483
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 182, 326
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 121
BpuMI CCSGG 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 251, 469, 477
Bse1I ACTGG 1 cut(s) 583
BseDI CCNNGG 3 cut(s) 251, 469, 477
BseMII CTCAG 4 cut(s) 94, 135, 311, 551
BseNI ACTGG 1 cut(s) 583
BseXI GCAGC 3 cut(s) 46, 112, 157
BseYI CCCAGC 1 cut(s) 557
BsgI GTGCAG 1 cut(s) 30
BshFI GGCC 4 cut(s) 42, 106, 234, 468
BshNI GGYRCC 1 cut(s) 473
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 1 cut(s) 251
BsmAI GTCTC 1 cut(s) 145
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 4 cut(s) 42, 106, 234, 468
Bso31I GGTCTC 1 cut(s) 145
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 2 cut(s) 277, 562
BspANI GGCC 4 cut(s) 42, 106, 234, 468
BspCNI CTCAG 4 cut(s) 93, 134, 310, 550
BspLI GGNNCC 4 cut(s) 21, 186, 475, 483
BspPI GGATC 2 cut(s) 272, 570
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 145
BsrI ACTGG 1 cut(s) 583
BssECI CCNNGG 3 cut(s) 251, 469, 477
BssMI GATC 2 cut(s) 277, 562
BssT1I CCWWGG 2 cut(s) 469, 477
Bst4CI ACNGT 2 cut(s) 24, 412
BstDEI CTNAG 4 cut(s) 80, 121, 297, 537
BstKTI GATC 2 cut(s) 280, 565
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 2 cut(s) 277, 562
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 46, 112, 157
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 4 cut(s) 42, 106, 234, 468
CaiI CAGNNNCTG 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 41, 105
Csp6I GTAC 1 cut(s) 287
CviAII CATG 2 cut(s) 38, 313
CviQI GTAC 1 cut(s) 287
DdeI CTNAG 4 cut(s) 80, 121, 297, 537
DpnI GATC 2 cut(s) 279, 564
DpnII GATC 2 cut(s) 277, 562
EaeI YGGCCR 1 cut(s) 466
Eco130I CCWWGG 2 cut(s) 469, 477
Eco147I AGGCCT 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 156
EcoT14I CCWWGG 2 cut(s) 469, 477
ErhI CCWWGG 2 cut(s) 469, 477
FaeI CATG 2 cut(s) 41, 316
FaiI YATR 5 cut(s) 39, 314, 335, 500, 502
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FatI CATG 2 cut(s) 37, 312
FauNDI CATATG 1 cut(s) 500
Fnu4HI GCNGC 3 cut(s) 35, 126, 171
Fsp4HI GCNGC 3 cut(s) 35, 126, 171
GluI GCNGC 3 cut(s) 35, 126, 171
GsaI CCCAGC 1 cut(s) 561
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 4 cut(s) 42, 106, 234, 468
HapII CCGG 1 cut(s) 251
Hin1II CATG 2 cut(s) 41, 316
HinfI GANTC 2 cut(s) 116, 137
HpaII CCGG 1 cut(s) 251
Hpy188I TCNGA 6 cut(s) 83, 277, 300, 540, 547, 567
Hpy188III TCNNGA 4 cut(s) 148, 195, 259, 281
HpyAV CCTTC 4 cut(s) 99, 224, 257, 437
HpyCH4III ACNGT 2 cut(s) 24, 412
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 5 cut(s) 47, 62, 128, 173, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 4 cut(s) 80, 121, 297, 537
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 2 cut(s) 41, 316
Kzo9I GATC 2 cut(s) 277, 562
LmnI GCTCC 5 cut(s) 31, 190, 346, 415, 481
LpnPI CCDG 8 cut(s) 57, 147, 208, 264, 294, 366, 500, 543
Lsp1109I GCAGC 3 cut(s) 46, 112, 157
LweI GCATC 2 cut(s) 182, 326
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 279, 564
MboI GATC 2 cut(s) 277, 562
MboII GAAGA 4 cut(s) 87, 258, 439, 452
MflI RGATCY 1 cut(s) 277
MlsI TGGCCA 1 cut(s) 468
MluNI TGGCCA 1 cut(s) 468
MlyI GAGTC 2 cut(s) 125, 146
MnlI CCTC 5 cut(s) 96, 130, 245, 255, 358
Mox20I TGGCCA 1 cut(s) 468
MscI TGGCCA 1 cut(s) 468
MseI TTAA 1 cut(s) 423
Msp20I TGGCCA 1 cut(s) 468
MspA1I CMGCKG 2 cut(s) 125, 170
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 559
MwoI GCNNNNNNNGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 252
NdeI CATATG 1 cut(s) 500
NdeII GATC 2 cut(s) 277, 562
NlaIII CATG 2 cut(s) 41, 316
NlaIV GGNNCC 4 cut(s) 21, 186, 475, 483
NmuCI GTSAC 1 cut(s) 355
PceI AGGCCT 1 cut(s) 234
PctI GAATGC 1 cut(s) 559
PkrI GCNGC 3 cut(s) 36, 127, 172
PleI GAGTC 2 cut(s) 124, 145
PpsI GAGTC 2 cut(s) 124, 145
PspFI CCCAGC 1 cut(s) 557
PspN4I GGNNCC 4 cut(s) 21, 186, 475, 483
PspPI GGNCC 2 cut(s) 41, 105
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 2 cut(s) 125, 170
RsaI GTAC 1 cut(s) 288
RsaNI GTAC 1 cut(s) 287
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 3 cut(s) 35, 126, 171
Sau3AI GATC 2 cut(s) 277, 562
Sau96I GGNCC 2 cut(s) 41, 105
SchI GAGTC 2 cut(s) 125, 146
ScrFI CCNGG 1 cut(s) 252
SfaNI GCATC 2 cut(s) 182, 326
SseBI AGGCCT 1 cut(s) 234
StuI AGGCCT 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 2 cut(s) 469, 477
TaaI ACNGT 2 cut(s) 24, 412
TaiI ACGT 1 cut(s) 345
TaqI TCGA 3 cut(s) 9, 140, 490
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 3 cut(s) 34, 125, 170
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 81, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.