Rw1G038820

R3H domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0020072
Physical Location & Seq
Reverse (-)
66365734 .. 66368262
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G038820.1

Sequence Viewer

Length: 657 bp
ATGGGAAGGAAGAAGAGCCAAAAAATTGAACAAACAACACCGCAATTTGACCGGAGGGCTCAGCATGGTCCTGCACTTTGGAATGGTGCATTTTTCACTCATCAGCTCAGAGAGCCTTCATCTTCAAATAGGCCCAAAAGAAAGTCCTCAGCTGCATTACCAGAGTCGAATGTCAAGAGATCCCGAGTTGCGTCAGCTGCATCATCATCGGCTCCCATCTCCAGAGCTAATGGTAAGGGGAAAGACAGAGCCAATGAAGGCCTCAAACCTTGCCTTCCGGGGTTCAAGAGGTCTTCTTTGTCAGATCCTGATGTACCTACTGCTCAGAATGTCAACAACATGGCATCTAACCCAATCAACATACCAAATGTGGAGCAACCTGTCATTAATGAGGCTTTAGAAACTCCGTTTGGGTTTGCAAAAAGTAGCACTTTTACTATGGAGCTTTTTAAGAAGATGGGATACGAAGAAGGGAGTGGCTTGGGAAAAGATGGCCAAGGCATTAAGGAGCCTATTCGAGTTGGCATATGGACAGAAAAGCAGGGATTGGGCTTCCAAATAGCTCAGACATCAGAGCAGAATGCTGGGGATCAGAATTCTTTCTTGCTTGCTTTCACTGGTGGCCTAGTGGGAACAGTTTCACTGGGATGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-patch R3H

Protein Analysis

218

Amino Acids

23.43

Weight (kDa)

9.76

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 145 - 184 1.8e-12 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020072)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0006267.1_g000005
rosa_roxburghii Rroxscaffold_4G00279320
rosa_samantha Rh1AG446000 Rh1BG401300 Rh1CG415700 Rh1DG431500
rosa_wichuraiana Rw1G038820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 41
AclWI GGATC 3 cut(s) 174, 299, 597
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 595
AfaI GTAC 1 cut(s) 315
AgsI TTSAA 3 cut(s) 29, 126, 286
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 6 cut(s) 106, 152, 197, 227, 445, 563
AluI AGCT 6 cut(s) 106, 152, 197, 227, 445, 563
AlwI GGATC 3 cut(s) 174, 299, 597
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 183
AoxI GGCC 4 cut(s) 131, 259, 493, 622
ApeKI GCWGC 2 cut(s) 152, 197
ApoI RAATTY 1 cut(s) 595
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 2 cut(s) 599, 637
AspS9I GGNCC 2 cut(s) 68, 132
AsuC2I CCSGG 1 cut(s) 279
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 1 cut(s) 68
BalI TGGCCA 1 cut(s) 495
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 446, 478
BbsI GAAGAC 1 cut(s) 285
BbvCI CCTCAGC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 139, 184
BccI CCATC 3 cut(s) 224, 451, 485
BcgI CGANNNNNNTGC 2 cut(s) 189, 223
BciVI GTATCC 1 cut(s) 455
BcnI CCSGG 1 cut(s) 279
BfaI CTAG 1 cut(s) 626
BfuI GTATCC 1 cut(s) 455
BisI GCNGC 2 cut(s) 153, 198
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 2 cut(s) 154, 199
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 68
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 68, 132
BmiI GGNNCC 2 cut(s) 213, 510
BmrFI CCNGG 1 cut(s) 279
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 2 cut(s) 209, 353
BmuI ACTGGG 1 cut(s) 653
BpiI GAAGAC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 205
Bpu10I CCTNAGC 1 cut(s) 148
Bpu1102I GCTNAGC 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 278, 496
BsaWI WCCGGW 1 cut(s) 51
Bse1I ACTGG 2 cut(s) 622, 648
BseDI CCNNGG 2 cut(s) 278, 496
BseGI GGATG 1 cut(s) 653
BseMII CTCAG 5 cut(s) 74, 121, 162, 338, 578
BseNI ACTGG 2 cut(s) 622, 648
BseXI GCAGC 2 cut(s) 139, 184
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 4 cut(s) 133, 261, 495, 624
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 2 cut(s) 52, 278
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 4 cut(s) 133, 261, 495, 624
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 3 cut(s) 179, 304, 589
Bsp1720I GCTNAGC 1 cut(s) 60
BspACI CCGC 1 cut(s) 41
BspANI GGCC 4 cut(s) 133, 261, 495, 624
BspCNI CTCAG 5 cut(s) 73, 120, 161, 337, 577
BspLI GGNNCC 2 cut(s) 213, 510
BspPI GGATC 3 cut(s) 174, 299, 597
BspQI GCTCTTC 1 cut(s) 8
BsrI ACTGG 2 cut(s) 622, 648
BssECI CCNNGG 2 cut(s) 278, 496
BssMI GATC 3 cut(s) 179, 304, 589
BssT1I CCWWGG 1 cut(s) 496
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 5 cut(s) 60, 107, 148, 324, 564
BstF5I GGATG 1 cut(s) 653
BstKTI GATC 3 cut(s) 182, 307, 592
BstMBI GATC 3 cut(s) 179, 304, 589
BstMWI GCNNNNNNNGC 2 cut(s) 112, 197
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 2 cut(s) 139, 184
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 179, 304
BstYI RGATCY 2 cut(s) 179, 304
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 4 cut(s) 133, 261, 495, 624
BtsCI GGATG 1 cut(s) 653
BtsIMutI CAGTG 2 cut(s) 615, 641
Cac8I GCNNGC 1 cut(s) 609
CaiI CAGNNNCTG 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 68, 132
CseI GACGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 314
CviAII CATG 2 cut(s) 65, 340
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 5 cut(s) 60, 107, 148, 324, 564
DpnI GATC 3 cut(s) 181, 306, 591
DpnII GATC 3 cut(s) 179, 304, 589
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 496
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 2 cut(s) 68, 343
FaiI YATR 6 cut(s) 66, 341, 362, 440, 527, 529
FalI AAGNNNNNCTT 2 cut(s) 415, 447
FatI CATG 2 cut(s) 64, 339
FauNDI CATATG 1 cut(s) 527
Fnu4HI GCNGC 2 cut(s) 153, 198
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 153, 198
FspBI CTAG 1 cut(s) 626
GluI GCNGC 2 cut(s) 153, 198
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 1 cut(s) 205
HaeIII GGCC 4 cut(s) 133, 261, 495, 624
HapII CCGG 2 cut(s) 52, 278
HgaI GACGC 1 cut(s) 180
Hin1II CATG 2 cut(s) 68, 343
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 1 cut(s) 164
HpaII CCGG 2 cut(s) 52, 278
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 6 cut(s) 110, 304, 327, 567, 574, 594
Hpy188III TCNNGA 5 cut(s) 175, 183, 222, 286, 308
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 4 cut(s) 126, 251, 284, 464
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4V TGCA 5 cut(s) 74, 89, 155, 200, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 197
HpyF3I CTNAG 5 cut(s) 60, 107, 148, 324, 564
Hsp92II CATG 2 cut(s) 68, 343
Kzo9I GATC 3 cut(s) 179, 304, 589
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 4 cut(s) 217, 373, 442, 508
Lsp1109I GCAGC 2 cut(s) 139, 184
LweI GCATC 2 cut(s) 209, 353
MaeI CTAG 1 cut(s) 626
MalI GATC 3 cut(s) 181, 306, 591
MboI GATC 3 cut(s) 179, 304, 589
MboII GAAGA 6 cut(s) 22, 25, 114, 285, 466, 479
MflI RGATCY 2 cut(s) 179, 304
MhlI GDGCHC 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 3 cut(s) 24, 44, 595
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 5 cut(s) 48, 157, 272, 282, 385
Mox20I TGGCCA 1 cut(s) 495
MroXI GAANNNNTTC 2 cut(s) 599, 637
MscI TGGCCA 1 cut(s) 495
MseI TTAA 3 cut(s) 387, 450, 504
MslI CAYNNNNRTG 1 cut(s) 646
Msp20I TGGCCA 1 cut(s) 495
MspA1I CMGCKG 2 cut(s) 152, 197
MspI CCGG 2 cut(s) 52, 278
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 586
MwoI GCNNNNNNNGC 2 cut(s) 112, 197
NciI CCSGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 527
NdeII GATC 3 cut(s) 179, 304, 589
NlaIII CATG 2 cut(s) 68, 343
NlaIV GGNNCC 2 cut(s) 213, 510
PceI AGGCCT 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 2 cut(s) 599, 637
PkrI GCNGC 2 cut(s) 154, 199
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PshBI ATTAAT 1 cut(s) 387
PspFI CCCAGC 1 cut(s) 584
PspN4I GGNNCC 2 cut(s) 213, 510
PspPI GGNCC 2 cut(s) 68, 132
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 2 cut(s) 179, 304
PvuII CAGCTG 2 cut(s) 152, 197
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 646
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 387, 450, 504
SatI GCNGC 2 cut(s) 153, 198
Sau3AI GATC 3 cut(s) 179, 304, 589
Sau96I GGNCC 2 cut(s) 68, 132
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 1 cut(s) 61
SfaNI GCATC 2 cut(s) 209, 353
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 646
Sse9I AATT 3 cut(s) 24, 44, 595
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 1 cut(s) 41
SspMI CTAG 1 cut(s) 626
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 637
TaqI TCGA 2 cut(s) 167, 517
TasI AATT 3 cut(s) 24, 44, 595
Tru1I TTAA 3 cut(s) 387, 450, 504
Tru9I TTAA 3 cut(s) 387, 450, 504
TscAI CASTG 2 cut(s) 622, 648
TseI GCWGC 2 cut(s) 152, 197
TspDTI ATGAA 2 cut(s) 108, 270
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 2 cut(s) 622, 648
VpaK11BI GGWCC 1 cut(s) 68
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 1 cut(s) 595
XmnI GAANNNNTTC 2 cut(s) 599, 637
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.