Rroxscaffold_4G00296350

nephrocystin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016392
Physical Location & Seq
Forward (+)
16402324 .. 16405221
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296350.1

Sequence Viewer

Length: 1740 bp
ATGTCAGCTTTGGTGTCGCTGAAGTCGTCGCCTTTGAGGACCCCGGCGCGGTCGGTTTTCGGACCCGACCCGGTTCACTCAAACTGGTCGAATGGCTTCACCAAAACCCCATCGCCTTCCGCCTCACGCGCCGCCCGACCAGTCAAAAAGGCCGCCGCTCAACCCGTCGACGAGGCCTCGCTGGACAACCCGGATCTGGGTCCCTTCCTGCTGAAGCTCGCCAGAGAGACGGTCGGGTCGGGCGAGAACCCGAACAAGGCTTTGGACTACGCTGTTCGGGCCTCCAAGTCATTCGAGCTGTGTTCGGGTCCCGGCCTTGACCTGGCCATGAGCCTGCACCTGGTGGCGGCGATTTACTGTAATTTGGGCCGGTTTGAGGAGGCGACTCGGGTTCTGGAGCGGTCCATTGAGGTCTCGGATCCCGAAAAAGGGTCGGATCATGCTCTGGCCAAGTTCTCCGGCTACATGCAGCTGGGTGACACGTACTCTATGCTGGGACAGCTTGAGCAGTCTATAATGTGCTACGGGTCGGGTCTGAAGATCCAGATGGACACTCTGGGTGAGTCCGACCCGAGAGTTGCAGAGACTTGCAGGTACCTAGCGGAGGCACATGTCCAAGTTATGCAATTTGATGAAGCAGAGAACTATAGTATGAAGACTCTTGAAATTCACAGGGAGCATAAGGGACCTGCGTCTATTGAAGAAGCTGCTGACCGTCGTCTCATGGCTCTCATCTATGAGGCGAAGGGAGATTATGAATCTGCACTCGAGCACCTTGTCCTTGCTAGCATGCTAATGATTGCCAATGGGCAAGACAATGAGGTTGCCGCTATTGATATGGGGATTGGTGACATTTACTTGTCTCTTTGTCGCTTTGACGAGGCTGTTTTCTCCTACCAGAAAGCGCTCACAGTCTTCAAATCTACAAGGGGTGAAAATCATCCTTCTGTGGCATCAGTCTTTATTCGCCTTGCTGATCTGTATTACAAGACAGGGAAGTTAAGAGAGTCTAAATCCTACTGTGAGAATGCCTTGAGGATATATGCTAAACCTTTGCCCGGAATTGTCTCTGAGGAAATTGCCACCGGGTTGACTGAAATTGCAGCCATTTATGAAGCTGTGAATGAGCCTGAAGAGGCACTAAAGCTCTTGCAAAAGGCCATGAAGTTACTGGAGGATACCCCTGCACACCGGAGCACTATTGCAGGAATAGAAGCACAGATGGGTGTTATGTTCTACATGCTTGGGAGGTTTGGGGAAGCTTGGAGTTCATTGGACAGTGCTATAGAGAAGCTTCGGGCCAGTGGGGAGAGGAAATCAGCCTATTTTGGTATTGTGCTGAACCAATTGGGATTGTCTTGTGTGCAGTTGTACAAATTAGCTGAGGCTGCTCGACTCTTTGAAGAAGCAAGGGATATACTTGAGCAGGAGTGTGGCATTTATCACTTGGATACTCTTGGAGTATATAGCAACCTTGCAGCAACTTATGATGCCATGGGAAGGGTAGAATCTGCAATTGAGATATTAGAGTACATTCTGAAGGTGAGGGAAGAAAAGCTTGGGACGGCAAATCCAGATGTTGATGATGAGAAGAAAAGATTAGCTGATCTCTTGAAAGAAGCAGGAAGGGCGCGGAACAGAAAGGGAAAATCGCTTGAAAATCTACTCGGTGGCTCCAACTCCCAAAGGATGAAGAAGGATGGAATGAAGAAGTGGTCTGGAACTAGTTTCAAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001655 GO:0001822 GO:0001889 GO:0001894 GO:0001895 GO:0001947 GO:0002009 GO:0003007 GO:0003143 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003341 GO:0003351 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005929 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007275 GO:0007368 GO:0007369 GO:0007389 GO:0007507 GO:0008150 GO:0009653 GO:0009790 GO:0009799 GO:0009855 GO:0009887 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010646 GO:0010648 GO:0016043 GO:0022603 GO:0022607 GO:0023051 GO:0023057 GO:0030030 GO:0030031 GO:0030111 GO:0030178 GO:0030323 GO:0030324 GO:0031016 GO:0032501 GO:0032502 GO:0035050 GO:0035239 GO:0035295 GO:0035469 GO:0042592 GO:0042995 GO:0043226 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0044782 GO:0045494 GO:0045595 GO:0045596 GO:0045995 GO:0048496 GO:0048513 GO:0048519 GO:0048523 GO:0048562 GO:0048565 GO:0048568 GO:0048583 GO:0048585 GO:0048598 GO:0048729 GO:0048731 GO:0048732 GO:0048856 GO:0048871 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051239 GO:0051960 GO:0055123 GO:0060026 GO:0060027 GO:0060249 GO:0060271 GO:0060287 GO:0060429 GO:0060541 GO:0060562 GO:0060828 GO:0060993 GO:0061008 GO:0061371 GO:0065007 GO:0065008 GO:0070925 GO:0071840 GO:0071907 GO:0071908 GO:0071909 GO:0071910 GO:0072001 GO:0072189 GO:0072359 GO:0090090 GO:0090175 GO:0090178 GO:0120025 GO:0120031 GO:0120036 GO:1905276 GO:1905330 GO:2000026 GO:2000027 GO:2000050 GO:2000095 GO:2000167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

579

Amino Acids

63.73

Weight (kDa)

5.55

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 108 - 181 1e-08 Tetratricopeptide repeat
TPR_MalT PF17874 112 - 266 6.1e-09 MalT-like TPR region
TPR_12 PF13424 155 - 225 1.2e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 155 - 255 1e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 199 - 259 2.7e-07 Tetratricopeptide repeat
TPR_MalT PF17874 242 - 409 1.9e-11 MalT-like TPR region
TPR_NPHP3 PF24885 273 - 404 2.2e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 275 - 349 1.1e-11 Tetratricopeptide repeat
TPR_12 PF13424 318 - 392 5.3e-10 Tetratricopeptide repeat
TPR_12 PF13424 445 - 517 8.2e-10 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016392)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16910
malus_domestica MD01G1083900.v1.1 MD07G1152700.v1.1
prunus_persica Prupe.2G191200_v2.0.a1
pyrus_communis pycom07g15090
rosa_chinensis RchiOBHm_Chr1g0360141
rosa_laevigata RLG00000027827
rosa_multiflora Rmu_sc0007813.1_g000005 Rmu_ssc0000368.1_g000043
rosa_roxburghii Rroxscaffold_4G00296350
rosa_rugosa Rorug01G0275300
rosa_samantha Rh1AG289100 Rh1BG254800 Rh1DG284200
rosa_wichuraiana Rw1G025650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 235
Acc36I ACCTGC 2 cut(s) 582, 697
Acc65I GGTACC 1 cut(s) 594
AccB1I GGYRCC 1 cut(s) 594
AccBSI CCGCTC 2 cut(s) 158, 400
AccI GTMKAC 1 cut(s) 168
AccII CGCG 3 cut(s) 49, 129, 1633
AclWI GGATC 5 cut(s) 201, 413, 426, 444, 535
AcoI YGGCCR 2 cut(s) 324, 447
AcsI RAATTY 1 cut(s) 666
AcuI CTGAAG 5 cut(s) 41, 233, 557, 1152, 1559
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 4 cut(s) 485, 596, 1373, 1532
AfeI AGCGCT 1 cut(s) 906
AflIII ACRYGT 2 cut(s) 480, 610
AgsI TTSAA 7 cut(s) 665, 701, 919, 1403, 1615, 1658, 1732
AhlI ACTAGT 1 cut(s) 1724
AjnI CCWGG 2 cut(s) 321, 339
AjuI GAANNNNNNNTTGG 4 cut(s) 245, 277, 1542, 1574
Alw21I GWGCWC 2 cut(s) 774, 1199
Alw26I GTCTC 6 cut(s) 221, 418, 578, 725, 867, 1072
AlwI GGATC 5 cut(s) 201, 413, 426, 444, 535
Ama87I CYCGRG 3 cut(s) 387, 571, 767
Aor51HI AGCGCT 1 cut(s) 906
AoxI GGCC 9 cut(s) 150, 174, 279, 313, 324, 367, 447, 1158, 1299
ApeKI GCWGC 5 cut(s) 469, 707, 1103, 1388, 1478
ApoI RAATTY 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 95
Asp718I GGTACC 1 cut(s) 594
AspLEI GCGC 4 cut(s) 49, 131, 907, 1633
AspS9I GGNCC 9 cut(s) 39, 62, 200, 279, 308, 367, 402, 686, 1299
AsuC2I CCSGG 6 cut(s) 44, 71, 191, 312, 1059, 1087
AsuHPI GGTGA 6 cut(s) 91, 488, 572, 860, 944, 1555
AsuNHI GCTAGC 1 cut(s) 785
AvaI CYCGRG 3 cut(s) 387, 571, 767
AvaII GGWCC 6 cut(s) 39, 62, 200, 308, 402, 686
BalI TGGCCA 2 cut(s) 326, 449
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 594
BbsI GAAGAC 2 cut(s) 662, 907
Bbv12I GWGCWC 2 cut(s) 774, 1199
BbvCI CCTCAGC 1 cut(s) 1383
BbvI GCAGC 5 cut(s) 481, 694, 1115, 1375, 1490
BccI CCATC 4 cut(s) 118, 541, 1216, 1694
BceAI ACGGC 1 cut(s) 1581
BciT130I CCWGG 2 cut(s) 323, 341
BciVI GTATCC 2 cut(s) 1171, 1444
BcnI CCSGG 6 cut(s) 44, 71, 191, 312, 1059, 1087
BcoDI GTCTC 6 cut(s) 221, 418, 578, 725, 867, 1072
BcuI ACTAGT 1 cut(s) 1724
BfaI CTAG 3 cut(s) 599, 786, 1725
BfmI CTRYAG 2 cut(s) 646, 1284
BfoI RGCGCY 1 cut(s) 908
BfuAI ACCTGC 2 cut(s) 582, 697
BfuI GTATCC 2 cut(s) 1171, 1444
Bme1390I CCNGG 8 cut(s) 44, 71, 191, 312, 323, 341, 1059, 1087
Bme18I GGWCC 6 cut(s) 39, 62, 200, 308, 402, 686
BmeT110I CYCGRG 3 cut(s) 387, 571, 767
BmgT120I GGNCC 9 cut(s) 39, 62, 200, 279, 308, 367, 402, 686, 1299
BmrFI CCNGG 8 cut(s) 44, 71, 191, 312, 323, 341, 1059, 1087
BmsI GCATC 2 cut(s) 962, 1480
BmtI GCTAGC 1 cut(s) 789
BoxI GACNNNNGTC 2 cut(s) 691, 717
BpiI GAAGAC 2 cut(s) 662, 907
BpmI CTGGAG 2 cut(s) 416, 1193
Bpu10I CCTNAGC 1 cut(s) 1383
BpuEI CTTGAG 3 cut(s) 524, 1054, 1442
BpuMI CCSGG 6 cut(s) 44, 71, 191, 312, 1059, 1087
BsaAI YACGTR 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 42, 1494
BsaWI WCCGGW 1 cut(s) 1191
Bse118I RCCGGY 1 cut(s) 369
Bse1I ACTGG 4 cut(s) 89, 140, 1176, 1302
BseBI CCWGG 2 cut(s) 323, 341
BseDI CCNNGG 2 cut(s) 42, 1494
BseGI GGATG 3 cut(s) 940, 1695, 1705
BseMII CTCAG 2 cut(s) 1062, 1374
BseNI ACTGG 4 cut(s) 89, 140, 1176, 1302
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 5 cut(s) 481, 694, 1115, 1375, 1490
BseYI CCCAGC 2 cut(s) 472, 493
BsgI GTGCAG 4 cut(s) 320, 747, 1170, 1385
Bsh1236I CGCG 3 cut(s) 49, 129, 1633
Bsh1285I CGRYCG 2 cut(s) 53, 234
BshFI GGCC 9 cut(s) 152, 176, 281, 315, 326, 369, 449, 1160, 1301
BshNI GGYRCC 1 cut(s) 594
BsiEI CGRYCG 2 cut(s) 53, 234
BsiHKAI GWGCWC 2 cut(s) 774, 1199
BsiHKCI CYCGRG 3 cut(s) 387, 571, 767
BsiSI CCGG 9 cut(s) 44, 71, 191, 312, 370, 459, 1059, 1086, 1192
BslFI GGGAC 5 cut(s) 186, 294, 510, 699, 1576
BsmAI GTCTC 6 cut(s) 221, 418, 578, 725, 867, 1072
BsmBI CGTCTC 2 cut(s) 221, 725
BsmFI GGGAC 5 cut(s) 186, 294, 510, 699, 1576
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 9 cut(s) 152, 176, 281, 315, 326, 369, 449, 1160, 1301
Bso31I GGTCTC 1 cut(s) 418
BsoBI CYCGRG 3 cut(s) 387, 571, 767
Bsp1286I GDGCHC 2 cut(s) 774, 1199
Bsp1407I TGTACA 1 cut(s) 1371
Bsp143I GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
Bsp19I CCATGG 1 cut(s) 1494
BspANI GGCC 9 cut(s) 152, 176, 281, 315, 326, 369, 449, 1160, 1301
BspCNI CTCAG 2 cut(s) 1063, 1375
BspFNI CGCG 3 cut(s) 49, 129, 1633
BspMI ACCTGC 2 cut(s) 582, 697
BspOI GCTAGC 1 cut(s) 789
BspPI GGATC 5 cut(s) 201, 413, 426, 444, 535
BspT107I GGYRCC 1 cut(s) 594
BspTNI GGTCTC 1 cut(s) 418
BsrBI CCGCTC 2 cut(s) 158, 400
BsrFI RCCGGY 1 cut(s) 369
BsrGI TGTACA 1 cut(s) 1371
BsrI ACTGG 4 cut(s) 89, 140, 1176, 1302
BssAI RCCGGY 1 cut(s) 369
BssECI CCNNGG 2 cut(s) 42, 1494
BssMI GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
BssT1I CCWWGG 1 cut(s) 1494
Bst2UI CCWGG 2 cut(s) 323, 341
Bst4CI ACNGT 6 cut(s) 232, 359, 716, 913, 1022, 1280
Bst6I CTCTTC 1 cut(s) 1128
BstAUI TGTACA 1 cut(s) 1371
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 4 cut(s) 219, 335, 787, 791
BstDEI CTNAG 2 cut(s) 1071, 1383
BstDSI CCRYGG 1 cut(s) 1494
BstENI CCTNNNNNAGG 1 cut(s) 602
BstF5I GGATG 3 cut(s) 940, 1695, 1705
BstFNI CGCG 3 cut(s) 49, 129, 1633
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 4 cut(s) 49, 131, 907, 1633
BstKTI GATC 6 cut(s) 196, 421, 439, 543, 979, 1609
BstMAI GTCTC 6 cut(s) 221, 418, 578, 725, 867, 1072
BstMBI GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
BstMCI CGRYCG 2 cut(s) 53, 234
BstMWI GCNNNNNNNGC 5 cut(s) 128, 278, 499, 1388, 1628
BstNI CCWGG 2 cut(s) 323, 341
BstNSI RCATGY 4 cut(s) 469, 614, 793, 1243
BstPAI GACNNNNGTC 2 cut(s) 691, 717
BstSCI CCNGG 8 cut(s) 42, 69, 189, 310, 321, 339, 1057, 1085
BstSFI CTRYAG 2 cut(s) 646, 1284
BstUI CGCG 3 cut(s) 49, 129, 1633
BstV1I GCAGC 5 cut(s) 481, 694, 1115, 1375, 1490
BstV2I GAAGAC 2 cut(s) 662, 907
BstX2I RGATCY 3 cut(s) 193, 418, 540
BstYI RGATCY 3 cut(s) 193, 418, 540
BsuI GTATCC 2 cut(s) 1171, 1444
BsuRI GGCC 9 cut(s) 152, 176, 281, 315, 326, 369, 449, 1160, 1301
BtgI CCRYGG 1 cut(s) 1494
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 3 cut(s) 940, 1695, 1705
BtsIMutI CAGTG 2 cut(s) 1285, 1309
BveI ACCTGC 2 cut(s) 582, 697
Cac8I GCNNGC 4 cut(s) 219, 335, 787, 791
CfoI GCGC 4 cut(s) 49, 131, 907, 1633
Cfr10I RCCGGY 1 cut(s) 369
Cfr13I GGNCC 9 cut(s) 39, 62, 200, 279, 308, 367, 402, 686, 1299
CseI GACGC 1 cut(s) 681
CsiI ACCWGGT 1 cut(s) 339
Csp6I GTAC 4 cut(s) 484, 595, 1372, 1531
CviAII CATG 9 cut(s) 328, 440, 466, 611, 724, 790, 1162, 1240, 1495
CviQI GTAC 4 cut(s) 484, 595, 1372, 1531
DdeI CTNAG 2 cut(s) 1071, 1383
DpnI GATC 6 cut(s) 195, 420, 438, 542, 978, 1608
DpnII GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
DraIII CACNNNGTG 1 cut(s) 343
DrdI GACNNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 2 cut(s) 324, 447
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
EciI GGCGGA 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 1494
Eco147I AGGCCT 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 418
Eco47I GGWCC 6 cut(s) 39, 62, 200, 308, 402, 686
Eco47III AGCGCT 1 cut(s) 906
Eco57I CTGAAG 5 cut(s) 41, 233, 557, 1152, 1559
Eco88I CYCGRG 3 cut(s) 387, 571, 767
EcoNI CCTNNNNNAGG 1 cut(s) 602
EcoO109I RGGNCCY 4 cut(s) 39, 200, 308, 686
EcoRII CCWGG 2 cut(s) 321, 339
EcoT14I CCWWGG 1 cut(s) 1494
ErhI CCWWGG 1 cut(s) 1494
Esp3I CGTCTC 2 cut(s) 221, 725
FaeI CATG 9 cut(s) 331, 443, 469, 614, 727, 793, 1165, 1243, 1498
FalI AAGNNNNNCTT 2 cut(s) 1542, 1574
FaqI GGGAC 5 cut(s) 186, 294, 510, 699, 1576
FatI CATG 9 cut(s) 327, 439, 465, 610, 723, 789, 1161, 1239, 1494
FblI GTMKAC 1 cut(s) 168
FokI GGATG 3 cut(s) 927, 1702, 1712
FspBI CTAG 3 cut(s) 599, 786, 1725
GlaI GCGC 4 cut(s) 48, 130, 906, 1632
GsaI CCCAGC 2 cut(s) 476, 497
GsuI CTGGAG 2 cut(s) 416, 1193
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 9 cut(s) 152, 176, 281, 315, 326, 369, 449, 1160, 1301
HapII CCGG 9 cut(s) 44, 71, 191, 312, 370, 459, 1059, 1086, 1192
HgaI GACGC 1 cut(s) 681
HhaI GCGC 4 cut(s) 49, 131, 907, 1633
Hin1II CATG 9 cut(s) 331, 443, 469, 614, 727, 793, 1165, 1243, 1498
Hin6I GCGC 4 cut(s) 47, 129, 905, 1631
HinP1I GCGC 4 cut(s) 47, 129, 905, 1631
HincII GTYRAC 2 cut(s) 169, 1092
HindII GTYRAC 2 cut(s) 169, 1092
HindIII AAGCTT 3 cut(s) 1260, 1292, 1556
HinfI GANTC 7 cut(s) 385, 563, 658, 758, 1007, 1395, 1508
HpaII CCGG 9 cut(s) 44, 71, 191, 312, 370, 459, 1059, 1086, 1192
HphI GGTGA 6 cut(s) 91, 488, 572, 860, 944, 1555
Hpy166II GTNNAC 3 cut(s) 76, 169, 1092
Hpy188I TCNGA 7 cut(s) 62, 418, 436, 537, 568, 1072, 1539
Hpy188III TCNNGA 7 cut(s) 395, 422, 544, 662, 1574, 1612, 1719
Hpy8I GTNNAC 3 cut(s) 76, 169, 1092
Hpy99I CGWCG 4 cut(s) 31, 170, 173, 720
HpyAV CCTTC 8 cut(s) 126, 214, 739, 954, 1494, 1534, 1620, 1690
HpyCH4III ACNGT 6 cut(s) 232, 359, 716, 913, 1022, 1280
HpyCH4IV ACGT 1 cut(s) 482
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 278, 499, 1388, 1628
HpyF3I CTNAG 2 cut(s) 1071, 1383
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 9 cut(s) 331, 443, 469, 614, 727, 793, 1165, 1243, 1498
HspAI GCGC 4 cut(s) 47, 129, 905, 1631
KflI GGGWCCC 2 cut(s) 200, 308
KpnI GGTACC 1 cut(s) 598
Kzo9I GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
LmnI GCTCC 4 cut(s) 397, 676, 1194, 1679
Lsp1109I GCAGC 5 cut(s) 481, 694, 1115, 1375, 1490
LweI GCATC 2 cut(s) 962, 1480
MabI ACCWGGT 1 cut(s) 339
MaeI CTAG 3 cut(s) 599, 786, 1725
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 476, 848, 1167
MalI GATC 6 cut(s) 195, 420, 438, 542, 978, 1608
MbiI CCGCTC 2 cut(s) 158, 400
MboI GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
MfeI CAATTG 2 cut(s) 1346, 1515
MflI RGATCY 3 cut(s) 193, 418, 540
MhlI GDGCHC 2 cut(s) 774, 1199
MlsI TGGCCA 2 cut(s) 326, 449
MluCI AATT 9 cut(s) 361, 626, 666, 1062, 1077, 1098, 1346, 1376, 1515
MluNI TGGCCA 2 cut(s) 326, 449
MlyI GAGTC 5 cut(s) 379, 572, 652, 1016, 1389
MmeI TCCRAC 3 cut(s) 414, 591, 1701
Mox20I TGGCCA 2 cut(s) 326, 449
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 2 cut(s) 326, 449
MseI TTAA 1 cut(s) 1001
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 2 cut(s) 326, 449
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 9 cut(s) 44, 71, 191, 312, 370, 459, 1059, 1086, 1192
MspR9I CCNGG 8 cut(s) 44, 71, 191, 312, 323, 341, 1059, 1087
MunI CAATTG 2 cut(s) 1346, 1515
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 2 cut(s) 323, 341
MvnI CGCG 3 cut(s) 49, 129, 1633
MwoI GCNNNNNNNGC 5 cut(s) 128, 278, 499, 1388, 1628
NciI CCSGG 6 cut(s) 44, 71, 191, 312, 1059, 1087
NcoI CCATGG 1 cut(s) 1494
NdeII GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
NheI GCTAGC 1 cut(s) 785
NlaIII CATG 9 cut(s) 331, 443, 469, 614, 727, 793, 1165, 1243, 1498
NmuCI GTSAC 2 cut(s) 476, 848
NspI RCATGY 4 cut(s) 469, 614, 793, 1243
PaeI GCATGC 1 cut(s) 793
PaeR7I CTCGAG 1 cut(s) 767
PceI AGGCCT 1 cut(s) 176
PciI ACATGT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 133, 240
PctI GAATGC 1 cut(s) 1033
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 2 cut(s) 758, 1508
PleI GAGTC 5 cut(s) 379, 571, 652, 1015, 1389
PpsI GAGTC 5 cut(s) 379, 571, 652, 1015, 1389
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 4 cut(s) 39, 200, 308, 686
PscI ACATGT 1 cut(s) 610
PshAI GACNNNNGTC 2 cut(s) 691, 717
Psp5II RGGWCCY 4 cut(s) 39, 200, 308, 686
Psp6I CCWGG 2 cut(s) 321, 339
PspFI CCCAGC 2 cut(s) 472, 493
PspGI CCWGG 2 cut(s) 321, 339
PspPI GGNCC 9 cut(s) 39, 62, 200, 279, 308, 367, 402, 686, 1299
PspPPI RGGWCCY 4 cut(s) 39, 200, 308, 686
PspXI VCTCGAGB 1 cut(s) 767
PsuI RGATCY 3 cut(s) 193, 418, 540
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 4 cut(s) 485, 596, 1373, 1532
RsaNI GTAC 4 cut(s) 484, 595, 1372, 1531
RseI CAYNNNNRTG 1 cut(s) 794
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 1 cut(s) 1001
Sau3AI GATC 6 cut(s) 193, 418, 436, 540, 976, 1606
Sau96I GGNCC 9 cut(s) 39, 62, 200, 279, 308, 367, 402, 686, 1299
SchI GAGTC 5 cut(s) 379, 572, 652, 1016, 1389
ScrFI CCNGG 8 cut(s) 44, 71, 191, 312, 323, 341, 1059, 1087
SduI GDGCHC 2 cut(s) 774, 1199
SexAI ACCWGGT 1 cut(s) 339
SfaNI GCATC 2 cut(s) 962, 1480
SfcI CTRYAG 2 cut(s) 646, 1284
Sfr274I CTCGAG 1 cut(s) 767
SgrDI CGTCGACG 1 cut(s) 167
SinI GGWCC 6 cut(s) 39, 62, 200, 308, 402, 686
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 4 cut(s) 503, 767, 1033, 1421
SmoI CTYRAG 4 cut(s) 503, 767, 1033, 1421
SpeI ACTAGT 1 cut(s) 1724
SphI GCATGC 1 cut(s) 793
Sse9I AATT 9 cut(s) 361, 626, 666, 1062, 1077, 1098, 1346, 1376, 1515
SseBI AGGCCT 1 cut(s) 176
SspMI CTAG 3 cut(s) 599, 786, 1725
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 8 cut(s) 42, 69, 189, 310, 321, 339, 1057, 1085
StyI CCWWGG 1 cut(s) 1494
TaaI ACNGT 6 cut(s) 232, 359, 716, 913, 1022, 1280
TaiI ACGT 1 cut(s) 485
TaqI TCGA 5 cut(s) 89, 168, 294, 768, 1393
TasI AATT 9 cut(s) 361, 626, 666, 1062, 1077, 1098, 1346, 1376, 1515
TatI WGTACW 2 cut(s) 1371, 1530
TauI GCSGC 5 cut(s) 134, 155, 158, 350, 830
TfiI GAWTC 2 cut(s) 758, 1508
Tru1I TTAA 1 cut(s) 1001
Tru9I TTAA 1 cut(s) 1001
TscAI CASTG 2 cut(s) 1285, 1309
TseFI GTSAC 2 cut(s) 476, 848
TseI GCWGC 5 cut(s) 469, 707, 1103, 1388, 1478
Tsp45I GTSAC 2 cut(s) 476, 848
TspDTI ATGAA 8 cut(s) 648, 668, 771, 1128, 1178, 1260, 1706, 1721
TspRI CASTG 2 cut(s) 1285, 1309
VpaK11BI GGWCC 6 cut(s) 39, 62, 200, 308, 402, 686
XagI CCTNNNNNAGG 1 cut(s) 602
XapI RAATTY 1 cut(s) 666
XceI RCATGY 4 cut(s) 469, 614, 793, 1243
XcmI CCANNNNNNNNNTGG 1 cut(s) 1168
XhoI CTCGAG 1 cut(s) 767
XmiI GTMKAC 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 3 cut(s) 599, 786, 1725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.