Rroxscaffold_4G00299110

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011701
Physical Location & Seq
Forward (+)
19041884 .. 19044862
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299110.1

Sequence Viewer

Length: 1497 bp
ATGAAAGACAACCCAAAAGACCCAGAAAACCTCTACCCAGAAAACCTCTACTCAACATGGATCTTCCCAAAGGTAACAATATGCTTCCTCCTCTTGATCTCTTTCAGCTACATCTCCTACTCTCTGAAATTTCTCCACCCATATTTATGTACCCAACAATCTCAATCTCTTAATTTCCAATCCCAGTTTTCCATACCCCAAACAGCTGATCCAAACCCACCAACGTCCCAAGAACACAAAACAAACATCTCCCACATCGTTTTTGGCATTGCAGCCTCAGCCGATCTCTGGCATCACAGAAAGAACTACATCAAGCTGTGGTGGAGACCAGACCAAATGAGAGGCCATGTTTGGCTAGACCAACCCGTAAAAACAGACCCCAAATCCGATGACAACCTTTTACCGCCGTTCAAAATCTCAGGCGACACGTCCGGCTTCAACTATACAAACCCAAAAGGCCACCACTCGGCTATAAGAATCTCCCGGATTGTTTCCGAGAGTTTCAGGCTGGGGATGAAGAATGTGAGGTGGTTTGTGCTGGGAGACGACGACACCGTTTTCGTGACCGAGAATCTGGTCAGGGTTTTGCAGAAGTATGATCATAACCAGTTTTACTATGTCGGAGGATCGTCGGAGAGTCACTTGCAGAACATAAAGTTTTCGTATAATATGGCTTATGGTGGAGGTGGGATTGCTATAAGTTACTCTTTAGCAAAAGCCCTTGAGAAAATGCAGGACAGTTGTATTCAGAGGTATCCTGGTTTGTACGGCTCCGATGATCGGCTTCAGGCTTGTATGGCTGAGATTGGTGTCCCCCTCACCAGAGAAAAAGGGTTTCATCAGTTTGATGTATATGGGAGCTTGTTCGGCCTCCTAGCAGCTCATCCAATCGCACCCCTAGTGTCACTACACCACCTAGATGTGGTTGAACCCATATTCCCCAATATGGATCGGATCCATGCCTTACAACTGCTCGAGGCTCCAACGAAGCTCGACTCAGCGGGGCTTGCGCAACAATCCATTTGCTACGATATCAATCGGTCCTGGACCATATCTGTTTCCTGGGGCTACGCAGTTCAAATATTTCAGGGTATTTCCTCGGCTCGAGAAATGGAACTGCCACTCAGGACCTTCCTCAATTGGTACAAGAGAGCTGATCATACTGCCTACACATTCAATACCGCCTCGGTCAATAGGCACGCATGTCAAGTACCGTTTGTATATTACTTATCTAATGCTTTCTACGATACAAGTACAAATCAAACAATCACTGAGTATGTTCAACATCAAACACGGATGCCAGAATGCAATTGGAAGATGGCAAATCCCTCCCGGATTCACAAAGTGCACGTCCATAAATCCTGCGACCCGCATTTATGGGACAAGGCCCCGAGGAGAAATTGTTGCAGGGTATTCCGACCAGATAGCAAGAAGAAAGGCACCATGGTCATTGAAGTAGGAGTATGCCATGAAGATGAAGTGCTCAAAGTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604 Malectin_like

Protein Analysis

498

Amino Acids

57.27

Weight (kDa)

8.35

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 156 - 281 1e-15 Fringe-like
DUF604 PF04646 216 - 469 2.9e-113 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011701)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01570 AT1G01570
fragaria_vesca FvH4_7g14990 FvH4_7g14990
prunus_persica Prupe.2G174500_v2.0.a1
pyrus_communis pycom01g09760
rosa_chinensis RchiOBHm_Chr1g0357031
rosa_laevigata RLG00000028059
rosa_multiflora Rmu_sc0000166.1_g000039 Rmu_sc0013980.1_g000025 Rmu_sc0035147.1_g000001
rosa_roxburghii Rroxscaffold_4G00299110
rosa_rugosa Rorug01G0253600 Rorug01G0253700
rosa_samantha Rh1AG267400 Rh1BG235200 Rh1CG251400 Rh1DG262600
rosa_wichuraiana Rw1G023610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1011
AccB1I GGYRCC 1 cut(s) 1439
AciI CCGC 4 cut(s) 404, 1001, 1182, 1370
AclWI GGATC 6 cut(s) 68, 203, 634, 949, 957, 962
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 770
AdeI CACNNNGTG 1 cut(s) 1345
AfaI GTAC 5 cut(s) 151, 767, 1145, 1212, 1255
AfiI CCNNNNNNNGG 5 cut(s) 288, 466, 780, 922, 946
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 7 cut(s) 412, 439, 929, 1079, 1177, 1283, 1454
AjiI CACGTC 2 cut(s) 429, 1351
AjnI CCWGG 3 cut(s) 757, 1043, 1061
AloI GAACNNNNNNTCC 2 cut(s) 921, 953
AluBI AGCT 7 cut(s) 108, 206, 316, 861, 881, 991, 1154
AluI AGCT 7 cut(s) 108, 206, 316, 861, 881, 991, 1154
Alw21I GWGCWC 2 cut(s) 1350, 1485
Alw26I GTCTC 2 cut(s) 319, 537
Alw44I GTGCAC 1 cut(s) 1346
AlwI GGATC 6 cut(s) 68, 203, 634, 949, 957, 962
Ama87I CYCGRG 3 cut(s) 974, 1104, 1390
AoxI GGCC 4 cut(s) 343, 457, 868, 1386
ApaLI GTGCAC 1 cut(s) 1346
ApeKI GCWGC 2 cut(s) 272, 878
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 4 cut(s) 542, 574, 1335, 1367
AspLEI GCGC 1 cut(s) 1012
AspS9I GGNCC 4 cut(s) 1041, 1047, 1128, 1387
AsuC2I CCSGG 2 cut(s) 484, 1333
AsuHPI GGTGA 1 cut(s) 811
AvaI CYCGRG 3 cut(s) 974, 1104, 1390
AvaII GGWCC 3 cut(s) 1041, 1047, 1128
BaeGI GKGCMC 1 cut(s) 1350
BamHI GGATCC 1 cut(s) 954
BanI GGYRCC 1 cut(s) 1439
Bbv12I GWGCWC 2 cut(s) 1350, 1485
BbvCI CCTCAGC 1 cut(s) 277
BbvI GCAGC 2 cut(s) 284, 890
BccI CCATC 1 cut(s) 1312
BceAI ACGGC 2 cut(s) 391, 784
BciT130I CCWGG 3 cut(s) 759, 1045, 1063
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 2 cut(s) 598, 1156
BcnI CCSGG 2 cut(s) 484, 1333
BcoDI GTCTC 2 cut(s) 319, 537
BfaI CTAG 4 cut(s) 356, 875, 899, 917
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 2 cut(s) 273, 879
BlsI GCNGC 2 cut(s) 274, 880
Bme1390I CCNGG 5 cut(s) 484, 759, 1045, 1063, 1333
Bme18I GGWCC 3 cut(s) 1041, 1047, 1128
BmeT110I CYCGRG 3 cut(s) 974, 1104, 1390
BmgBI CACGTC 2 cut(s) 429, 1351
BmgT120I GGNCC 4 cut(s) 1041, 1047, 1128, 1387
BmiI GGNNCC 5 cut(s) 772, 956, 981, 1389, 1441
BmrFI CCNGG 5 cut(s) 484, 759, 1045, 1063, 1333
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 301, 1287
BmuI ACTGGG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 2 cut(s) 484, 1333
BsaBI GATNNNNATC 1 cut(s) 1035
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 5 cut(s) 1062, 1098, 1185, 1391, 1443
BsaXI ACNNNNNCTCC 2 cut(s) 1387, 1417
Bsc4I CCNNNNNNNGG 5 cut(s) 288, 466, 780, 922, 946
Bse1I ACTGG 2 cut(s) 184, 607
Bse3DI GCAATG 1 cut(s) 267
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 759, 1045, 1063
BseDI CCNNGG 5 cut(s) 1062, 1098, 1185, 1391, 1443
BseGI GGATG 3 cut(s) 519, 883, 1302
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 5 cut(s) 288, 466, 780, 922, 946
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 6 cut(s) 291, 432, 792, 1011, 1138, 1263
BseNI ACTGG 2 cut(s) 184, 607
BseRI GAGGAG 2 cut(s) 80, 1408
BseSI GKGCMC 1 cut(s) 1350
BseXI GCAGC 2 cut(s) 284, 890
BseYI CCCAGC 2 cut(s) 508, 538
BshFI GGCC 4 cut(s) 345, 459, 870, 1388
BshNI GGYRCC 1 cut(s) 1439
BsiHKAI GWGCWC 2 cut(s) 1350, 1485
BsiHKCI CYCGRG 3 cut(s) 974, 1104, 1390
BsiSI CCGG 3 cut(s) 432, 484, 1333
BslFI GGGAC 3 cut(s) 211, 797, 1394
BslI CCNNNNNNNGG 5 cut(s) 288, 466, 780, 922, 946
BsmAI GTCTC 2 cut(s) 319, 537
BsmBI CGTCTC 1 cut(s) 537
BsmFI GGGAC 3 cut(s) 211, 797, 1394
BsmI GAATGC 1 cut(s) 1310
BsnI GGCC 4 cut(s) 345, 459, 870, 1388
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 3 cut(s) 974, 1104, 1390
Bsp1286I GDGCHC 2 cut(s) 1350, 1485
Bsp19I CCATGG 1 cut(s) 1443
BspACI CCGC 4 cut(s) 404, 1001, 1182, 1370
BspANI GGCC 4 cut(s) 345, 459, 870, 1388
BspCNI CTCAG 6 cut(s) 290, 431, 793, 1010, 1137, 1264
BspLI GGNNCC 5 cut(s) 772, 956, 981, 1389, 1441
BspPI GGATC 6 cut(s) 68, 203, 634, 949, 957, 962
BspT107I GGYRCC 1 cut(s) 1439
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 267
BsrI ACTGG 2 cut(s) 184, 607
BssECI CCNNGG 5 cut(s) 1062, 1098, 1185, 1391, 1443
BssT1I CCWWGG 1 cut(s) 1443
Bst2UI CCWGG 3 cut(s) 759, 1045, 1063
Bst4CI ACNGT 3 cut(s) 556, 740, 1215
BstC8I GCNNGC 2 cut(s) 1008, 1200
BstDEI CTNAG 6 cut(s) 277, 418, 801, 997, 1124, 1272
BstDSI CCRYGG 1 cut(s) 1443
BstF5I GGATG 3 cut(s) 519, 883, 1302
BstHHI GCGC 1 cut(s) 1012
BstMAI GTCTC 2 cut(s) 319, 537
BstMWI GCNNNNNNNGC 4 cut(s) 278, 797, 867, 1007
BstNI CCWGG 3 cut(s) 759, 1045, 1063
BstNSI RCATGY 1 cut(s) 1206
BstSCI CCNGG 5 cut(s) 482, 757, 1043, 1061, 1331
BstSLI GKGCMC 1 cut(s) 1350
BstV1I GCAGC 2 cut(s) 284, 890
BstX2I RGATCY 2 cut(s) 60, 954
BstYI RGATCY 2 cut(s) 60, 954
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 4 cut(s) 345, 459, 870, 1388
BtgI CCRYGG 1 cut(s) 1443
BtrI CACGTC 2 cut(s) 429, 1351
BtsCI GGATG 3 cut(s) 519, 883, 1302
BtsIMutI CAGTG 1 cut(s) 1269
Cac8I GCNNGC 2 cut(s) 1008, 1200
CfoI GCGC 1 cut(s) 1012
Cfr13I GGNCC 4 cut(s) 1041, 1047, 1128, 1387
Csp6I GTAC 5 cut(s) 150, 766, 1144, 1211, 1254
CviAII CATG 6 cut(s) 57, 347, 959, 1203, 1444, 1469
CviQI GTAC 5 cut(s) 150, 766, 1144, 1211, 1254
DdeI CTNAG 6 cut(s) 277, 418, 801, 997, 1124, 1272
DraIII CACNNNGTG 1 cut(s) 1345
Eco130I CCWWGG 1 cut(s) 1443
Eco31I GGTCTC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 3 cut(s) 1041, 1047, 1128
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 3 cut(s) 974, 1104, 1390
EcoO109I RGGNCCY 2 cut(s) 1128, 1387
EcoRII CCWGG 3 cut(s) 757, 1043, 1061
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 1 cut(s) 1443
ErhI CCWWGG 1 cut(s) 1443
Esp3I CGTCTC 1 cut(s) 537
FaeI CATG 6 cut(s) 60, 350, 962, 1206, 1447, 1472
FalI AAGNNNNNCTT 2 cut(s) 691, 723
FaqI GGGAC 3 cut(s) 211, 797, 1394
FatI CATG 6 cut(s) 56, 346, 958, 1202, 1443, 1468
FauI CCCGC 2 cut(s) 994, 1377
FbaI TGATCA 2 cut(s) 598, 1156
Fnu4HI GCNGC 2 cut(s) 273, 879
FokI GGATG 3 cut(s) 526, 870, 1309
Fsp4HI GCNGC 2 cut(s) 273, 879
FspBI CTAG 4 cut(s) 356, 875, 899, 917
FspI TGCGCA 1 cut(s) 1011
GlaI GCGC 1 cut(s) 1011
GluI GCNGC 2 cut(s) 273, 879
GsaI CCCAGC 2 cut(s) 512, 542
HaeIII GGCC 4 cut(s) 345, 459, 870, 1388
HapII CCGG 3 cut(s) 432, 484, 1333
HhaI GCGC 1 cut(s) 1012
Hin1II CATG 6 cut(s) 60, 350, 962, 1206, 1447, 1472
Hin6I GCGC 1 cut(s) 1010
HinP1I GCGC 1 cut(s) 1010
HinfI GANTC 5 cut(s) 477, 571, 637, 995, 1336
HpaII CCGG 3 cut(s) 432, 484, 1333
HphI GGTGA 1 cut(s) 811
Hpy166II GTNNAC 1 cut(s) 1348
Hpy188I TCNGA 9 cut(s) 126, 388, 496, 623, 634, 750, 775, 954, 1418
Hpy188III TCNNGA 4 cut(s) 94, 562, 1106, 1126
Hpy8I GTNNAC 1 cut(s) 1348
Hpy99I CGWCG 2 cut(s) 551, 634
HpyAV CCTTC 1 cut(s) 1141
HpyCH4III ACNGT 3 cut(s) 556, 740, 1215
HpyCH4IV ACGT 3 cut(s) 224, 428, 1350
HpyCH4V TGCA 7 cut(s) 272, 589, 646, 733, 1308, 1348, 1407
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 797, 867, 1007
HpyF3I CTNAG 6 cut(s) 277, 418, 801, 997, 1124, 1272
HpySE526I ACGT 3 cut(s) 224, 428, 1350
Hsp92II CATG 6 cut(s) 60, 350, 962, 1206, 1447, 1472
HspAI GCGC 1 cut(s) 1010
Ksp22I TGATCA 2 cut(s) 598, 1156
LmnI GCTCC 3 cut(s) 776, 858, 985
Lsp1109I GCAGC 2 cut(s) 284, 890
LweI GCATC 2 cut(s) 301, 1287
MaeI CTAG 4 cut(s) 356, 875, 899, 917
MaeII ACGT 3 cut(s) 224, 428, 1350
MaeIII GTNAC 5 cut(s) 73, 562, 638, 701, 903
MboII GAAGA 5 cut(s) 55, 529, 1327, 1444, 1484
MfeI CAATTG 2 cut(s) 1138, 1309
MflI RGATCY 2 cut(s) 60, 954
MhlI GDGCHC 2 cut(s) 1350, 1485
MluCI AATT 5 cut(s) 128, 172, 1138, 1309, 1399
MlyI GAGTC 2 cut(s) 646, 989
MmeI TCCRAC 4 cut(s) 601, 612, 1007, 1441
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 3 cut(s) 145, 918, 1473
MspA1I CMGCKG 2 cut(s) 206, 1001
MspI CCGG 3 cut(s) 432, 484, 1333
MspR9I CCNGG 5 cut(s) 484, 759, 1045, 1063, 1333
MunI CAATTG 2 cut(s) 1138, 1309
Mva1269I GAATGC 1 cut(s) 1310
MvaI CCWGG 3 cut(s) 759, 1045, 1063
MwoI GCNNNNNNNGC 4 cut(s) 278, 797, 867, 1007
NciI CCSGG 2 cut(s) 484, 1333
NcoI CCATGG 1 cut(s) 1443
NlaIII CATG 6 cut(s) 60, 350, 962, 1206, 1447, 1472
NlaIV GGNNCC 5 cut(s) 772, 956, 981, 1389, 1441
NmeAIII GCCGAG 2 cut(s) 446, 1079
NmuCI GTSAC 3 cut(s) 562, 638, 903
NsbI TGCGCA 1 cut(s) 1011
NspI RCATGY 1 cut(s) 1206
PaeR7I CTCGAG 2 cut(s) 974, 1104
PcsI WCGNNNNNNNCGW 1 cut(s) 552
PctI GAATGC 1 cut(s) 1310
PfeI GAWTC 3 cut(s) 477, 571, 1336
PfoI TCCNGGA 3 cut(s) 482, 1043, 1331
PkrI GCNGC 2 cut(s) 274, 880
PleI GAGTC 2 cut(s) 645, 989
PpsI GAGTC 2 cut(s) 645, 989
PpuMI RGGWCCY 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 1128
Psp6I CCWGG 3 cut(s) 757, 1043, 1061
PspFI CCCAGC 2 cut(s) 508, 538
PspGI CCWGG 3 cut(s) 757, 1043, 1061
PspN4I GGNNCC 5 cut(s) 772, 956, 981, 1389, 1441
PspPI GGNCC 4 cut(s) 1041, 1047, 1128, 1387
PspPPI RGGWCCY 1 cut(s) 1128
PspXI VCTCGAGB 1 cut(s) 974
PsuI RGATCY 2 cut(s) 60, 954
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 5 cut(s) 151, 767, 1145, 1212, 1255
RsaNI GTAC 5 cut(s) 150, 766, 1144, 1211, 1254
RseI CAYNNNNRTG 3 cut(s) 145, 918, 1473
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 2 cut(s) 273, 879
Sau96I GGNCC 4 cut(s) 1041, 1047, 1128, 1387
SchI GAGTC 2 cut(s) 646, 989
ScrFI CCNGG 5 cut(s) 484, 759, 1045, 1063, 1333
SduI GDGCHC 2 cut(s) 1350, 1485
SfaNI GCATC 2 cut(s) 301, 1287
Sfr274I CTCGAG 2 cut(s) 974, 1104
SinI GGWCC 3 cut(s) 1041, 1047, 1128
SlaI CTCGAG 2 cut(s) 974, 1104
SmiMI CAYNNNNRTG 3 cut(s) 145, 918, 1473
SmlI CTYRAG 3 cut(s) 722, 974, 1104
SmoI CTYRAG 3 cut(s) 722, 974, 1104
Sse9I AATT 5 cut(s) 128, 172, 1138, 1309, 1399
SsiI CCGC 4 cut(s) 404, 1001, 1182, 1370
SspI AATATT 1 cut(s) 1083
SspMI CTAG 4 cut(s) 356, 875, 899, 917
StyD4I CCNGG 5 cut(s) 482, 757, 1043, 1061, 1331
StyI CCWWGG 1 cut(s) 1443
TaaI ACNGT 3 cut(s) 556, 740, 1215
TaiI ACGT 3 cut(s) 227, 431, 1353
TaqI TCGA 3 cut(s) 975, 993, 1105
TaqII GACCGA 3 cut(s) 581, 1029, 1177
TasI AATT 5 cut(s) 128, 172, 1138, 1309, 1399
TatI WGTACW 1 cut(s) 1253
TfiI GAWTC 3 cut(s) 477, 571, 1336
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 1 cut(s) 1276
TseFI GTSAC 3 cut(s) 562, 638, 903
TseI GCWGC 2 cut(s) 272, 878
Tsp45I GTSAC 3 cut(s) 562, 638, 903
TspDTI ATGAA 5 cut(s) 17, 530, 827, 1485, 1491
TspGWI ACGGA 1 cut(s) 1309
TspRI CASTG 1 cut(s) 1276
VneI GTGCAC 1 cut(s) 1346
VpaK11BI GGWCC 3 cut(s) 1041, 1047, 1128
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 1206
XcmI CCANNNNNNNNNTGG 2 cut(s) 260, 1308
XhoI CTCGAG 2 cut(s) 974, 1104
XspI CTAG 4 cut(s) 356, 875, 899, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.