Rroxscaffold_4G00311890

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000817
Physical Location & Seq
Forward (+)
34474227 .. 34487173
12947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311890.1

Sequence Viewer

Length: 1485 bp
ATGTCTTCCAGTAAGAGTTCATCTCAACACTTAAAGGATGCTAGATTGCAGAGTCAAATTGCTGATGCTGTGAACAGGCATAACTTTGAAGCACATCGTTCTCCGAAAAGTTTAAGGGATGACAGTGATGTTCATCATAAAGATCAAGAAGCTACGGAACTTTATTCCCGAGCCAGAGCGCAGGAAGAAGAGATCCAATTTCTTCGTGGGCAAGTCACTGATGCCTGTGTGAAGGAATTGCGGCTGTTGAATGAGAAGTATGCATTGGAGAAGAAATTCGCTGACTTACGAATGGCAATTGATGAGAAGCAGAATGAAGCCACTACTTCTGCATTAAATGAGTTGGCCCGCAGAAAAGGTGATCTTGAAGAAAACTTGAAATTAACTCACGATTTGAAGGCTGCAGAGGATGAGAGATATGTCTTCATGTCATCCATGCTGGGGTTATTGGCTGAATATGGTATTTGGCCCCATGTCATAAATGCTTCTGCCATATCCAACAGTTTAAAGCATCTGCATGATGAATTGCAGTGGAAGATTAGAACTTCACATGAACAGCAAGGTTTTGATCGTTATACTGATGCTCAACATCTGGAGCCAACTGCAAACTTGCAGCAGTACAGGAATGATTTTACAGACGCGAAAAATTTGATGCTAATTAATAAAGAAAATCCACAACAGTTCACATCCAATATAGATAGTAATACAACTCACAGGAATATGGATGGCTTTATTCTGCATTATTCAGTTGATAAAGATGTTGTGAATGGGAGAGCTGAACAAATGACCAATGGCACCTCATATCCTCACACACCTGAGAATACATCCTCTATGTCGCAAGGGCCTGGCATAGATAATTTTCAGATAATTGGTGATGCTGTACCGGGAGGCAAACTTCTTGGATGTGGATTTCCAGTACGGGGGACCTCACTTTGCATGTTTCAGTGGGTTCGTCATCTTCAAGATGGCACCAGGGAGGTTATTGAAGGAGCTACAAATCCAGAATATGTAGTTACAGCTGACGATGTTGATAAAATTATTGCTGTTGACTGCATACCAATGGATGACCAAGGCCGCCAGGGGGAACTAGTGAGGCATTTTGCTAATGATCAGAACAAAATTAAATGTGACCCGGAGATGCAGCTGGAGATTGACACATATATTTCTAGAGGACAGGCCACTTTTAGTGTTCGACTACTGATGGATTCTGCGGAGAATTGGGAGCCAGCAACTTTATATCTGGGAAGGTCAGGCTATCGGGTCAAGATCAATAGCACAGAAGCTGTTGTTATTGCAGAGAAATTCTCAAATGATTTATCAATCAAAGTTCCTTGTGGCTTATCAACTCAATTTGTGATGACATGTTCTGATGGATCTTCTCATCCTTTCAACACACAGAGTGTTTGGATGCGCGATACTCTTGTGTTGACTATGAGAATGCTACAAACCAAGGCACTGGATGACAGAAGAAAAGGAAGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-IgPH

Protein Analysis

494

Amino Acids

55.98

Weight (kDa)

5.61

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IG_AIR9 PF23197 285 - 364 3.7e-11 AIR9 A9 domain
DUF7046 PF23080 406 - 491 1.3e-14 Domain of unknown function (DUF7046)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000817)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03560
fragaria_vesca FvH4_7g08080 FvH4_7g08080 FvH4_7g08080 FvH4_7g08080 FvH4_7g08080 FvH4_7g08080
malus_domestica MD07G1077700.v1.1
prunus_persica Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1
pyrus_communis pycom07g06140
rosa_chinensis RchiOBHm_Chr1g0341241
rosa_laevigata RLG00000029074
rosa_multiflora Rmu_sc0004092.1_g000040 Rmu_sc0042871.1_g000001
rosa_roxburghii Rroxscaffold_4G00311890
rosa_rugosa Rorug01G0158600.1 Rorug01G0158700.1 Rorug01G0158800.1 Rorug01G0158900.1 Rorug01G0159000.1
rosa_samantha Rh1AG172900 Rh1BG141000 Rh1CG161000 Rh1DG173300
rosa_wichuraiana Rw1G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 794, 968
AccII CGCG 2 cut(s) 641, 1413
AciI CCGC 4 cut(s) 241, 349, 1075, 1211
AclWI GGATC 2 cut(s) 187, 1381
AcsI RAATTY 3 cut(s) 275, 646, 1301
AdeI CACNNNGTG 1 cut(s) 1400
AfaI GTAC 3 cut(s) 620, 882, 918
AfiI CCNNNNNNNGG 3 cut(s) 441, 920, 1081
AflIII ACRYGT 1 cut(s) 1361
AgsI TTSAA 8 cut(s) 89, 250, 368, 379, 397, 962, 986, 1390
AhlI ACTAGT 1 cut(s) 1087
AjnI CCWGG 3 cut(s) 844, 971, 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 152, 776, 992, 1019, 1144, 1283
AluI AGCT 6 cut(s) 152, 776, 992, 1019, 1144, 1283
AlwI GGATC 2 cut(s) 187, 1381
AlwNI CAGNNNCTG 1 cut(s) 1283
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 5 cut(s) 345, 467, 842, 1072, 1176
ApeKI GCWGC 3 cut(s) 401, 613, 1141
ApoI RAATTY 3 cut(s) 275, 646, 1301
AseI ATTAAT 1 cut(s) 660
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 2 cut(s) 181, 1413
AspS9I GGNCC 4 cut(s) 346, 468, 842, 924
AsuC2I CCSGG 2 cut(s) 885, 1133
AsuHPI GGTGA 2 cut(s) 371, 884
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 1 cut(s) 924
BaeI ACNNNNGTAYC 2 cut(s) 1407, 1440
BanI GGYRCC 2 cut(s) 794, 968
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 388, 625, 1153
BccI CCATC 4 cut(s) 719, 959, 1195, 1364
BciT130I CCWGG 3 cut(s) 846, 973, 1079
BclI TGATCA 1 cut(s) 1108
BcnI CCSGG 2 cut(s) 885, 1133
BcuI ACTAGT 1 cut(s) 1087
BfaI CTAG 3 cut(s) 42, 1088, 1167
BfmI CTRYAG 1 cut(s) 402
BisI GCNGC 5 cut(s) 242, 402, 614, 1075, 1142
BlsI GCNGC 5 cut(s) 243, 403, 615, 1076, 1143
Bme1390I CCNGG 5 cut(s) 846, 885, 973, 1079, 1133
Bme18I GGWCC 1 cut(s) 924
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 4 cut(s) 346, 468, 842, 924
BmiI GGNNCC 6 cut(s) 470, 597, 796, 925, 970, 1224
BmrFI CCNGG 5 cut(s) 846, 885, 973, 1079, 1133
BmsI GCATC 9 cut(s) 28, 55, 211, 520, 571, 642, 865, 1128, 1398
BpiI GAAGAC 1 cut(s) 415
BplI GAGNNNNNCTC 4 cut(s) 7, 39, 1289, 1321
BpmI CTGGAG 2 cut(s) 614, 1166
BpuMI CCSGG 2 cut(s) 885, 1133
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 972, 1069, 1078, 1449
Bsc4I CCNNNNNNNGG 3 cut(s) 441, 920, 1081
Bse1I ACTGG 3 cut(s) 9, 914, 1461
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 3 cut(s) 846, 973, 1079
BseDI CCNNGG 4 cut(s) 972, 1069, 1078, 1449
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 3 cut(s) 441, 920, 1081
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 3 cut(s) 9, 914, 1461
BseXI GCAGC 3 cut(s) 388, 625, 1153
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 2 cut(s) 641, 1413
BshFI GGCC 5 cut(s) 347, 469, 844, 1074, 1178
BshNI GGYRCC 2 cut(s) 794, 968
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 2 cut(s) 884, 1133
BslFI GGGAC 1 cut(s) 937
BslI CCNNNNNNNGG 3 cut(s) 441, 920, 1081
BsmFI GGGAC 1 cut(s) 937
BsmI GAATGC 1 cut(s) 1443
BsnI GGCC 5 cut(s) 347, 469, 844, 1074, 1178
BsoBI CYCGRG 1 cut(s) 168
Bsp143I GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
BspACI CCGC 4 cut(s) 241, 349, 1075, 1211
BspANI GGCC 5 cut(s) 347, 469, 844, 1074, 1178
BspCNI CTCAG 1 cut(s) 808
BspFNI CGCG 2 cut(s) 641, 1413
BspLI GGNNCC 6 cut(s) 470, 597, 796, 925, 970, 1224
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 2 cut(s) 187, 1381
BspT107I GGYRCC 2 cut(s) 794, 968
BsrI ACTGG 3 cut(s) 9, 914, 1461
BssECI CCNNGG 4 cut(s) 972, 1069, 1078, 1449
BssMI GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
BssT1I CCWWGG 2 cut(s) 1069, 1449
Bst2UI CCWGG 3 cut(s) 846, 973, 1079
Bst4CI ACNGT 3 cut(s) 125, 503, 681
Bst6I CTCTTC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 349, 1227
BstDEI CTNAG 1 cut(s) 816
BstFNI CGCG 2 cut(s) 641, 1413
BstHHI GCGC 2 cut(s) 181, 1413
BstKTI GATC 7 cut(s) 145, 195, 364, 571, 1111, 1269, 1376
BstMBI GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
BstNI CCWGG 3 cut(s) 846, 973, 1079
BstNSI RCATGY 2 cut(s) 940, 1365
BstSCI CCNGG 5 cut(s) 844, 883, 971, 1077, 1131
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 2 cut(s) 641, 1413
BstV1I GCAGC 3 cut(s) 388, 625, 1153
BstV2I GAAGAC 1 cut(s) 415
BstX2I RGATCY 2 cut(s) 192, 1373
BstXI CCANNNNNNTGG 1 cut(s) 1456
BstYI RGATCY 2 cut(s) 192, 1373
BsuRI GGCC 5 cut(s) 347, 469, 844, 1074, 1178
BtsI GCAGTG 1 cut(s) 536
BtsIMutI CAGTG 5 cut(s) 130, 216, 536, 950, 1454
Cac8I GCNNGC 2 cut(s) 349, 1227
CaiI CAGNNNCTG 1 cut(s) 1283
CfoI GCGC 2 cut(s) 181, 1413
Cfr13I GGNCC 4 cut(s) 346, 468, 842, 924
CseI GACGC 1 cut(s) 647
Csp6I GTAC 3 cut(s) 619, 881, 917
CviAII CATG 7 cut(s) 427, 436, 473, 518, 551, 937, 1362
CviQI GTAC 3 cut(s) 619, 881, 917
DdeI CTNAG 1 cut(s) 816
DpnI GATC 7 cut(s) 144, 194, 363, 570, 1110, 1268, 1375
DpnII GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
DraI TTTAAA 1 cut(s) 507
DraIII CACNNNGTG 1 cut(s) 1400
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 2 cut(s) 1069, 1449
Eco47I GGWCC 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 168
EcoO109I RGGNCCY 2 cut(s) 842, 924
EcoRII CCWGG 3 cut(s) 844, 971, 1077
EcoT14I CCWWGG 2 cut(s) 1069, 1449
EcoT22I ATGCAT 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 1069, 1449
FaeI CATG 7 cut(s) 430, 439, 476, 521, 554, 940, 1365
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 937
FatI CATG 7 cut(s) 426, 435, 472, 517, 550, 936, 1361
FauI CCCGC 1 cut(s) 356
FbaI TGATCA 1 cut(s) 1108
Fnu4HI GCNGC 5 cut(s) 242, 402, 614, 1075, 1142
Fsp4HI GCNGC 5 cut(s) 242, 402, 614, 1075, 1142
FspBI CTAG 3 cut(s) 42, 1088, 1167
GlaI GCGC 2 cut(s) 180, 1412
GluI GCNGC 5 cut(s) 242, 402, 614, 1075, 1142
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 2 cut(s) 614, 1166
HaeIII GGCC 5 cut(s) 347, 469, 844, 1074, 1178
HapII CCGG 2 cut(s) 884, 1133
HgaI GACGC 1 cut(s) 647
HhaI GCGC 2 cut(s) 181, 1413
Hin1II CATG 7 cut(s) 430, 439, 476, 521, 554, 940, 1365
Hin6I GCGC 2 cut(s) 179, 1411
HinP1I GCGC 2 cut(s) 179, 1411
HincII GTYRAC 2 cut(s) 1048, 1428
HindII GTYRAC 2 cut(s) 1048, 1428
HinfI GANTC 2 cut(s) 52, 1205
HpaII CCGG 2 cut(s) 884, 1133
HphI GGTGA 2 cut(s) 371, 884
Hpy166II GTNNAC 4 cut(s) 73, 684, 1048, 1428
Hpy188I TCNGA 4 cut(s) 105, 864, 1113, 1369
Hpy188III TCNNGA 9 cut(s) 146, 168, 365, 389, 593, 962, 1001, 1167, 1264
Hpy8I GTNNAC 4 cut(s) 73, 684, 1048, 1428
HpyAV CCTTC 5 cut(s) 226, 391, 980, 1239, 1470
HpyCH4III ACNGT 3 cut(s) 125, 503, 681
HpyF3I CTNAG 1 cut(s) 816
Hsp92II CATG 7 cut(s) 430, 439, 476, 521, 554, 940, 1365
HspAI GCGC 2 cut(s) 179, 1411
Ksp22I TGATCA 1 cut(s) 1108
Kzo9I GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
LmnI GCTCC 3 cut(s) 595, 989, 1222
Lsp1109I GCAGC 3 cut(s) 388, 625, 1153
LweI GCATC 9 cut(s) 28, 55, 211, 520, 571, 642, 865, 1128, 1398
MaeI CTAG 3 cut(s) 42, 1088, 1167
MaeIII GTNAC 3 cut(s) 214, 1012, 1127
MalI GATC 7 cut(s) 144, 194, 363, 570, 1110, 1268, 1375
MboI GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 2 cut(s) 192, 1373
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 9 cut(s) 400, 808, 816, 838, 881, 937, 970, 1086, 1163
Mph1103I ATGCAT 1 cut(s) 265
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1122
MslI CAYNNNNRTG 2 cut(s) 516, 1058
MspA1I CMGCKG 2 cut(s) 1019, 1144
MspI CCGG 2 cut(s) 884, 1133
MspR9I CCNGG 5 cut(s) 846, 885, 973, 1079, 1133
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 1443
MvaI CCWGG 3 cut(s) 846, 973, 1079
MvnI CGCG 2 cut(s) 641, 1413
NciI CCSGG 2 cut(s) 885, 1133
NdeII GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
NlaIII CATG 7 cut(s) 430, 439, 476, 521, 554, 940, 1365
NlaIV GGNNCC 6 cut(s) 470, 597, 796, 925, 970, 1224
NmuCI GTSAC 2 cut(s) 214, 1127
NsiI ATGCAT 1 cut(s) 265
NspI RCATGY 2 cut(s) 940, 1365
PciI ACATGT 1 cut(s) 1361
PctI GAATGC 1 cut(s) 1443
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 1205
PkrI GCNGC 5 cut(s) 243, 403, 615, 1076, 1143
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PpuMI RGGWCCY 1 cut(s) 924
PscI ACATGT 1 cut(s) 1361
PshBI ATTAAT 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 924
Psp6I CCWGG 3 cut(s) 844, 971, 1077
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 3 cut(s) 844, 971, 1077
PspN4I GGNNCC 6 cut(s) 470, 597, 796, 925, 970, 1224
PspPI GGNCC 4 cut(s) 346, 468, 842, 924
PspPPI RGGWCCY 1 cut(s) 924
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 1283
PsuI RGATCY 2 cut(s) 192, 1373
PvuII CAGCTG 2 cut(s) 1019, 1144
RsaI GTAC 3 cut(s) 620, 882, 918
RsaNI GTAC 3 cut(s) 619, 881, 917
RseI CAYNNNNRTG 2 cut(s) 516, 1058
SaqAI TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1122
SatI GCNGC 5 cut(s) 242, 402, 614, 1075, 1142
Sau3AI GATC 7 cut(s) 142, 192, 361, 568, 1108, 1266, 1373
Sau96I GGNCC 4 cut(s) 346, 468, 842, 924
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 5 cut(s) 846, 885, 973, 1079, 1133
SfaNI GCATC 9 cut(s) 28, 55, 211, 520, 571, 642, 865, 1128, 1398
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 1 cut(s) 924
SmiMI CAYNNNNRTG 2 cut(s) 516, 1058
SpeI ACTAGT 1 cut(s) 1087
SsiI CCGC 4 cut(s) 241, 349, 1075, 1211
SspMI CTAG 3 cut(s) 42, 1088, 1167
StyD4I CCNGG 5 cut(s) 844, 883, 971, 1077, 1131
StyI CCWWGG 2 cut(s) 1069, 1449
TaaI ACNGT 3 cut(s) 125, 503, 681
TaqI TCGA 1 cut(s) 1192
TatI WGTACW 1 cut(s) 618
TauI GCSGC 2 cut(s) 244, 1077
TfiI GAWTC 1 cut(s) 1205
Tru1I TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1122
Tru9I TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1122
TscAI CASTG 5 cut(s) 130, 223, 536, 950, 1461
TseFI GTSAC 2 cut(s) 214, 1127
TseI GCWGC 3 cut(s) 401, 613, 1141
Tsp45I GTSAC 2 cut(s) 214, 1127
TspDTI ATGAA 6 cut(s) 9, 122, 330, 415, 537, 567
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 5 cut(s) 130, 223, 536, 950, 1461
VpaK11BI GGWCC 1 cut(s) 924
VspI ATTAAT 1 cut(s) 660
XapI RAATTY 3 cut(s) 275, 646, 1301
XbaI TCTAGA 1 cut(s) 1166
XceI RCATGY 2 cut(s) 940, 1365
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 3 cut(s) 42, 1088, 1167
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.