Rh1AG172900

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000817
Physical Location & Seq
Reverse (-)
33147088 .. 33161913
14826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG172900.1

Sequence Viewer

Length: 1455 bp
ATGTCTTCCACTAAGAGTTCATCTCAACACTTAAAGGATGCTGGATTGCAGAGTCAAATTGCTGATGCTGTGAACAGGCATAACTTTGAAGCACATCGTTCTCCGAAAAGTTTAAGGGATGACAGTGATGTTCATCATAAAGATCAAGAAGCTACGGAACTTTATTCCCGAGCCAGAGCGCAGGAAGAAGAGATCCAATTTCTTCGTGGGCAAGTCACTGATGCCTGTGTGAAGGAATTGCGGCTGTTGAACGAGAAGTATGCATTGGAGAAGAAATTCGCTGACTTACGAATGGCAATTGATGAGAAGCAGAATGAAGCCACTACTTCTGCATTAAATGAGTTGGCCCGCAGAAAAGGTGATCTTGAAGAAAACTTGAAATTAACTCACGATTTGAAGGCTGCAGATGATGAGAGATATGTCTTCATGTCATCCATGCTTGGGTTATTGGCTGAATATGGTATTTGGCCCCATGTCATAAATGCTTCTGCCATATCCAACAGTTTAAAGCATCTGCATGATGAATTGCAGTGGAAGATTAGAACTTCACATGAACAGCAACGTTTTGATCGTTACACTGATGCTCAACATCTGGAGCCAACTGCAAACTTGCAGCAGTACAGGAATGATTTTACAGACACAAAAAATTTGATGCTAATTAATAAAGAAAATCCACAACAGTTCAAATCCAATATAGATAGGAATATGGATGGCTTTATTCTGCATTATTCAGTTGATAAAGATGTTGTGAATGGGAGAGCTGAACAAATGACCAATGGCACCTCATATCTTCACACACCTGAGAATACATCCTCTATTTCGCAAGGGCCTGGCATAGATAATTTTCAGATAATTGGTGATGCTGTACCGGGAGGCAAACTTCTTGGATGTGGATTTCCAGTACGGGGGACCTCACTTTGCATGTTTCAGTGGGTTCGTTATCTTCAAGATGGCACCAGGGAGGTTATTGAAGGAGCTACAAATCCAGAATATGTAGTTACAGCTGACGATGTTGATAAAATTATTGCTGTTGACTGCATACCAATGGATGACCAAGGCCGCCAGGGGGAACTAGTGAGGCTTTTTGCTAATGAGCAGAACAAAATTAAATGTGACCCGAAGATGCAGCTGGAGATTGACACATATATTTCTAGAGGACAAGCCACTTTTAGTGTTCAACTACTGATGGATTCTGCTGAGAATTGGGAGCCAGCAACTTTATATCTGGGAAGGTCAGGCTATCGGATCAAGATCGATAGCACAGAAGCTGTTGTTATTGCAGAGAAATTCTCAAATGATTTATCAATCAAAGTTCCTTGTGGCCTCTCAACTCAATTTGTGATGACATGTTCTGATGGATCTTCTCATCCTTTCAACACACAGAGAGTTTGGATGCGCGATACTCTAGTGTTGACGATGAGAATGCTACAAAGCAAGGTATATTCTTTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAB-IgPH

Protein Analysis

484

Amino Acids

54.98

Weight (kDa)

5.46

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IG_AIR9 PF23197 280 - 359 1.6e-10 AIR9 A9 domain
DUF7046 PF23080 400 - 478 4.5e-13 Domain of unknown function (DUF7046)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000817)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03560
fragaria_vesca FvH4_7g08080 FvH4_7g08080 FvH4_7g08080 FvH4_7g08080 FvH4_7g08080 FvH4_7g08080
malus_domestica MD07G1077700.v1.1
prunus_persica Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1 Prupe.2G098900_v2.0.a1
pyrus_communis pycom07g06140
rosa_chinensis RchiOBHm_Chr1g0341241
rosa_laevigata RLG00000029074
rosa_multiflora Rmu_sc0004092.1_g000040 Rmu_sc0042871.1_g000001
rosa_roxburghii Rroxscaffold_4G00311890
rosa_rugosa Rorug01G0158600.1 Rorug01G0158700.1 Rorug01G0158800.1 Rorug01G0158900.1 Rorug01G0159000.1
rosa_samantha Rh1AG172900 Rh1BG141000 Rh1CG161000 Rh1DG173300
rosa_wichuraiana Rw1G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 779, 953
AccII CGCG 1 cut(s) 1398
AciI CCGC 3 cut(s) 241, 349, 1060
AclI AACGTT 1 cut(s) 562
AclWI GGATC 3 cut(s) 187, 1253, 1366
AcsI RAATTY 3 cut(s) 275, 646, 1286
AfaI GTAC 3 cut(s) 620, 867, 903
AfiI CCNNNNNNNGG 3 cut(s) 441, 905, 1066
AflIII ACRYGT 1 cut(s) 1346
AhlI ACTAGT 1 cut(s) 1072
AjnI CCWGG 3 cut(s) 829, 956, 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 152, 761, 977, 1004, 1129, 1268
AluI AGCT 6 cut(s) 152, 761, 977, 1004, 1129, 1268
AlwI GGATC 3 cut(s) 187, 1253, 1366
AlwNI CAGNNNCTG 1 cut(s) 1268
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 5 cut(s) 345, 467, 827, 1057, 1321
ApeKI GCWGC 3 cut(s) 401, 613, 1126
ApoI RAATTY 3 cut(s) 275, 646, 1286
AseI ATTAAT 1 cut(s) 660
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 2 cut(s) 181, 1398
AspS9I GGNCC 4 cut(s) 346, 468, 827, 909
AsuC2I CCSGG 1 cut(s) 870
AsuHPI GGTGA 2 cut(s) 371, 869
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 1 cut(s) 909
BaeI ACNNNNGTAYC 2 cut(s) 1392, 1425
BanI GGYRCC 2 cut(s) 779, 953
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 388, 625, 1138
BccI CCATC 4 cut(s) 704, 944, 1180, 1349
BcgI CGANNNNNNTGC 4 cut(s) 242, 276, 1405, 1439
BciT130I CCWGG 3 cut(s) 831, 958, 1064
BcnI CCSGG 1 cut(s) 870
BcuI ACTAGT 1 cut(s) 1072
BfaI CTAG 3 cut(s) 1073, 1152, 1406
BfmI CTRYAG 1 cut(s) 402
BisI GCNGC 5 cut(s) 242, 402, 614, 1060, 1127
BlsI GCNGC 5 cut(s) 243, 403, 615, 1061, 1128
Bme1390I CCNGG 4 cut(s) 831, 870, 958, 1064
Bme18I GGWCC 1 cut(s) 909
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 4 cut(s) 346, 468, 827, 909
BmiI GGNNCC 6 cut(s) 470, 597, 781, 910, 955, 1209
BmrFI CCNGG 4 cut(s) 831, 870, 958, 1064
BmsI GCATC 9 cut(s) 28, 55, 211, 520, 571, 642, 850, 1113, 1383
BpiI GAAGAC 1 cut(s) 415
BplI GAGNNNNNCTC 4 cut(s) 7, 39, 1274, 1306
BpmI CTGGAG 2 cut(s) 614, 1151
BpuMI CCSGG 1 cut(s) 870
Bsa29I ATCGAT 1 cut(s) 1254
BsaBI GATNNNNATC 2 cut(s) 132, 1250
BsaJI CCNNGG 3 cut(s) 957, 1054, 1063
Bsc4I CCNNNNNNNGG 3 cut(s) 441, 905, 1066
Bse1I ACTGG 1 cut(s) 899
Bse8I GATNNNNATC 2 cut(s) 132, 1250
BseBI CCWGG 3 cut(s) 831, 958, 1064
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 3 cut(s) 957, 1054, 1063
BseGI GGATG 9 cut(s) 43, 124, 431, 715, 809, 893, 1054, 1366, 1398
BseJI GATNNNNATC 2 cut(s) 132, 1250
BseLI CCNNNNNNNGG 3 cut(s) 441, 905, 1066
BseMII CTCAG 2 cut(s) 792, 1188
BseNI ACTGG 1 cut(s) 899
BseXI GCAGC 3 cut(s) 388, 625, 1138
Bsh1236I CGCG 1 cut(s) 1398
BshFI GGCC 5 cut(s) 347, 469, 829, 1059, 1323
BshNI GGYRCC 2 cut(s) 779, 953
BshVI ATCGAT 1 cut(s) 1254
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 1 cut(s) 869
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 3 cut(s) 441, 905, 1066
BsmFI GGGAC 1 cut(s) 922
BsmI GAATGC 1 cut(s) 1428
BsnI GGCC 5 cut(s) 347, 469, 829, 1059, 1323
BsoBI CYCGRG 1 cut(s) 168
Bsp143I GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
BspACI CCGC 3 cut(s) 241, 349, 1060
BspANI GGCC 5 cut(s) 347, 469, 829, 1059, 1323
BspCNI CTCAG 2 cut(s) 793, 1189
BspDI ATCGAT 1 cut(s) 1254
BspFNI CGCG 1 cut(s) 1398
BspLI GGNNCC 6 cut(s) 470, 597, 781, 910, 955, 1209
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 3 cut(s) 187, 1253, 1366
BspT107I GGYRCC 2 cut(s) 779, 953
BsrI ACTGG 1 cut(s) 899
BssECI CCNNGG 3 cut(s) 957, 1054, 1063
BssMI GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
BssT1I CCWWGG 1 cut(s) 1054
Bst2UI CCWGG 3 cut(s) 831, 958, 1064
Bst4CI ACNGT 3 cut(s) 125, 503, 681
Bst6I CTCTTC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 349, 1212
BstDEI CTNAG 3 cut(s) 12, 801, 1197
BstF5I GGATG 9 cut(s) 43, 124, 431, 715, 809, 893, 1054, 1366, 1398
BstFNI CGCG 1 cut(s) 1398
BstHHI GCGC 2 cut(s) 181, 1398
BstKTI GATC 7 cut(s) 145, 195, 364, 571, 1248, 1254, 1361
BstMBI GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
BstNI CCWGG 3 cut(s) 831, 958, 1064
BstNSI RCATGY 2 cut(s) 925, 1350
BstSCI CCNGG 4 cut(s) 829, 868, 956, 1062
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 1 cut(s) 1398
BstV1I GCAGC 3 cut(s) 388, 625, 1138
BstV2I GAAGAC 1 cut(s) 415
BstX2I RGATCY 2 cut(s) 192, 1358
BstYI RGATCY 2 cut(s) 192, 1358
Bsu15I ATCGAT 1 cut(s) 1254
BsuRI GGCC 5 cut(s) 347, 469, 829, 1059, 1323
BsuTUI ATCGAT 1 cut(s) 1254
BtsCI GGATG 9 cut(s) 43, 124, 431, 715, 809, 893, 1054, 1366, 1398
BtsI GCAGTG 1 cut(s) 536
BtsIMutI CAGTG 5 cut(s) 130, 216, 536, 576, 935
Cac8I GCNNGC 2 cut(s) 349, 1212
CaiI CAGNNNCTG 1 cut(s) 1268
CfoI GCGC 2 cut(s) 181, 1398
Cfr13I GGNCC 4 cut(s) 346, 468, 827, 909
ClaI ATCGAT 1 cut(s) 1254
Csp6I GTAC 3 cut(s) 619, 866, 902
CviAII CATG 7 cut(s) 427, 436, 473, 518, 551, 922, 1347
CviQI GTAC 3 cut(s) 619, 866, 902
DdeI CTNAG 3 cut(s) 12, 801, 1197
DpnI GATC 7 cut(s) 144, 194, 363, 570, 1247, 1253, 1360
DpnII GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
DraI TTTAAA 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 1054
Eco47I GGWCC 1 cut(s) 909
Eco88I CYCGRG 1 cut(s) 168
EcoO109I RGGNCCY 2 cut(s) 827, 909
EcoRII CCWGG 3 cut(s) 829, 956, 1062
EcoT14I CCWWGG 1 cut(s) 1054
EcoT22I ATGCAT 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 1054
FaeI CATG 7 cut(s) 430, 439, 476, 521, 554, 925, 1350
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 922
FatI CATG 7 cut(s) 426, 435, 472, 517, 550, 921, 1346
FauI CCCGC 1 cut(s) 356
Fnu4HI GCNGC 5 cut(s) 242, 402, 614, 1060, 1127
FokI GGATG 9 cut(s) 50, 131, 418, 722, 796, 900, 1061, 1353, 1405
Fsp4HI GCNGC 5 cut(s) 242, 402, 614, 1060, 1127
FspBI CTAG 3 cut(s) 1073, 1152, 1406
GlaI GCGC 2 cut(s) 180, 1397
GluI GCNGC 5 cut(s) 242, 402, 614, 1060, 1127
GsuI CTGGAG 2 cut(s) 614, 1151
HaeIII GGCC 5 cut(s) 347, 469, 829, 1059, 1323
HapII CCGG 1 cut(s) 869
HhaI GCGC 2 cut(s) 181, 1398
Hin1II CATG 7 cut(s) 430, 439, 476, 521, 554, 925, 1350
Hin6I GCGC 2 cut(s) 179, 1396
HinP1I GCGC 2 cut(s) 179, 1396
HincII GTYRAC 2 cut(s) 1033, 1413
HindII GTYRAC 2 cut(s) 1033, 1413
HinfI GANTC 2 cut(s) 52, 1190
HpaII CCGG 1 cut(s) 869
HphI GGTGA 2 cut(s) 371, 869
Hpy166II GTNNAC 3 cut(s) 73, 1033, 1413
Hpy188I TCNGA 4 cut(s) 105, 849, 1245, 1354
Hpy188III TCNNGA 9 cut(s) 146, 168, 365, 389, 593, 947, 986, 1152, 1249
Hpy8I GTNNAC 3 cut(s) 73, 1033, 1413
HpyAV CCTTC 4 cut(s) 226, 391, 965, 1224
HpyCH4III ACNGT 3 cut(s) 125, 503, 681
HpyCH4IV ACGT 1 cut(s) 562
HpyF3I CTNAG 3 cut(s) 12, 801, 1197
HpySE526I ACGT 1 cut(s) 562
Hsp92II CATG 7 cut(s) 430, 439, 476, 521, 554, 925, 1350
HspAI GCGC 2 cut(s) 179, 1396
Kzo9I GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
LmnI GCTCC 3 cut(s) 595, 974, 1207
Lsp1109I GCAGC 3 cut(s) 388, 625, 1138
LweI GCATC 9 cut(s) 28, 55, 211, 520, 571, 642, 850, 1113, 1383
MaeI CTAG 3 cut(s) 1073, 1152, 1406
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 4 cut(s) 214, 572, 997, 1112
MalI GATC 7 cut(s) 144, 194, 363, 570, 1247, 1253, 1360
MboI GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 2 cut(s) 192, 1358
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 8 cut(s) 793, 823, 866, 922, 955, 1071, 1148, 1334
Mph1103I ATGCAT 1 cut(s) 265
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1107
MslI CAYNNNNRTG 2 cut(s) 516, 1043
MspA1I CMGCKG 2 cut(s) 1004, 1129
MspI CCGG 1 cut(s) 869
MspR9I CCNGG 4 cut(s) 831, 870, 958, 1064
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 1428
MvaI CCWGG 3 cut(s) 831, 958, 1064
MvnI CGCG 1 cut(s) 1398
NciI CCSGG 1 cut(s) 870
NdeII GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
NlaIII CATG 7 cut(s) 430, 439, 476, 521, 554, 925, 1350
NlaIV GGNNCC 6 cut(s) 470, 597, 781, 910, 955, 1209
NmuCI GTSAC 2 cut(s) 214, 1112
NsiI ATGCAT 1 cut(s) 265
NspI RCATGY 2 cut(s) 925, 1350
PciI ACATGT 1 cut(s) 1346
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 1 cut(s) 1428
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 1190
PkrI GCNGC 5 cut(s) 243, 403, 615, 1061, 1128
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PpuMI RGGWCCY 1 cut(s) 909
PscI ACATGT 1 cut(s) 1346
PshBI ATTAAT 1 cut(s) 660
Psp1406I AACGTT 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 3 cut(s) 829, 956, 1062
PspGI CCWGG 3 cut(s) 829, 956, 1062
PspN4I GGNNCC 6 cut(s) 470, 597, 781, 910, 955, 1209
PspPI GGNCC 4 cut(s) 346, 468, 827, 909
PspPPI RGGWCCY 1 cut(s) 909
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 1268
PsuI RGATCY 2 cut(s) 192, 1358
PvuII CAGCTG 2 cut(s) 1004, 1129
RsaI GTAC 3 cut(s) 620, 867, 903
RsaNI GTAC 3 cut(s) 619, 866, 902
RseI CAYNNNNRTG 2 cut(s) 516, 1043
SaqAI TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1107
SatI GCNGC 5 cut(s) 242, 402, 614, 1060, 1127
Sau3AI GATC 7 cut(s) 142, 192, 361, 568, 1245, 1251, 1358
Sau96I GGNCC 4 cut(s) 346, 468, 827, 909
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 4 cut(s) 831, 870, 958, 1064
SfaNI GCATC 9 cut(s) 28, 55, 211, 520, 571, 642, 850, 1113, 1383
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 1 cut(s) 909
SmiMI CAYNNNNRTG 2 cut(s) 516, 1043
SpeI ACTAGT 1 cut(s) 1072
SsiI CCGC 3 cut(s) 241, 349, 1060
SspMI CTAG 3 cut(s) 1073, 1152, 1406
StyD4I CCNGG 4 cut(s) 829, 868, 956, 1062
StyI CCWWGG 1 cut(s) 1054
TaaI ACNGT 3 cut(s) 125, 503, 681
TaiI ACGT 1 cut(s) 565
TaqI TCGA 1 cut(s) 1254
TatI WGTACW 1 cut(s) 618
TauI GCSGC 2 cut(s) 244, 1062
TfiI GAWTC 1 cut(s) 1190
Tru1I TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1107
Tru9I TTAA 7 cut(s) 32, 113, 335, 383, 506, 660, 1107
TscAI CASTG 5 cut(s) 130, 223, 536, 583, 935
TseFI GTSAC 2 cut(s) 214, 1112
TseI GCWGC 3 cut(s) 401, 613, 1126
Tsp45I GTSAC 2 cut(s) 214, 1112
TspDTI ATGAA 6 cut(s) 9, 122, 330, 415, 537, 567
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 5 cut(s) 130, 223, 536, 583, 935
VpaK11BI GGWCC 1 cut(s) 909
VspI ATTAAT 1 cut(s) 660
XapI RAATTY 3 cut(s) 275, 646, 1286
XbaI TCTAGA 1 cut(s) 1151
XceI RCATGY 2 cut(s) 925, 1350
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 3 cut(s) 1073, 1152, 1406
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.