Rroxscaffold_4G00325960

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018562
Physical Location & Seq
Reverse (-)
58013151 .. 58016366
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325960.1

Sequence Viewer

Length: 432 bp
ATGGTCGAATTGGGATGCGGGGTTATCGAATGTCAAAAGTTGGTGTTTGTTGTCTCCGAGCTCAACAAGAAATTTCGGAAGAAGCCGGTGTTTCGAGCTCGATTCGAGAAAAGATTGGAAAATATAAGGCAACATGGAATACCATTAGTCATAGGCAAGCTCGAAGGGTTAAACTCTCTTCATTTCATTGCCAGCCATTTCCGGATCTTTTTGGCTCAGAAAGTGTTGATCTATAAGTGCAACATCCAAGGAAAACCTGTTGTCACGACAATACAGATGTTGGAACTAGCAATTGTTGAATATGTCGATGAATTGTACAAGTTCTACAAACTTGAAGAGGATGACTTGGACATTGATCAAGTCCGGGTTGAACGGGCAAGCATGCTGTCAAGTTTCAGATCACCGTGTTGTATGTCCGTGGTAGATCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

143

Amino Acids

16.64

Weight (kDa)

8.83

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 68 - 96 1.8e-06 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018562)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0353501
rosa_laevigata RLG00000012063
rosa_roxburghii Rroxscaffold_4G00325960 Rroxscaffold_5G00378580 Rroxscaffold_6G00410900
rosa_rugosa Rorug06G0205700
rosa_samantha Rh4CG126400 Rh5DG131500 Rh6CG219400 Rh6DG315700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 201
AciI CCGC 1 cut(s) 18
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 71
AfaI GTAC 1 cut(s) 317
AgsI TTSAA 3 cut(s) 299, 335, 371
AluBI AGCT 3 cut(s) 61, 98, 160
AluI AGCT 3 cut(s) 61, 98, 160
Alw21I GWGCWC 2 cut(s) 63, 100
Alw26I GTCTC 1 cut(s) 58
AlwI GGATC 1 cut(s) 212
Aor13HI TCCGGA 1 cut(s) 201
ApoI RAATTY 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 393
BanII GRGCYC 2 cut(s) 63, 100
Bbv12I GWGCWC 2 cut(s) 63, 100
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 365
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 1 cut(s) 5
BpuMI CCSGG 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 247, 417
BsaWI WCCGGW 1 cut(s) 201
Bse118I RCCGGY 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 186
BseAI TCCGGA 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 247, 417
BseGI GGATG 3 cut(s) 20, 243, 346
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 63, 100
BsiSI CCGG 3 cut(s) 86, 202, 364
BsmAI GTCTC 1 cut(s) 58
Bsp1286I GDGCHC 2 cut(s) 63, 100
Bsp13I TCCGGA 1 cut(s) 201
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 5 cut(s) 204, 228, 355, 398, 424
BspACI CCGC 1 cut(s) 18
BspCNI CTCAG 1 cut(s) 229
BspEI TCCGGA 1 cut(s) 201
BspPI GGATC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 247, 417
BssMI GATC 5 cut(s) 204, 228, 355, 398, 424
BssT1I CCWWGG 1 cut(s) 247
Bst4CI ACNGT 1 cut(s) 405
Bst6I CTCTTC 2 cut(s) 183, 330
BstAUI TGTACA 1 cut(s) 315
BstC8I GCNNGC 4 cut(s) 158, 193, 379, 383
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 3 cut(s) 20, 243, 346
BstKTI GATC 5 cut(s) 207, 231, 358, 401, 427
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 5 cut(s) 204, 228, 355, 398, 424
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 20, 243, 346
Cac8I GCNNGC 4 cut(s) 158, 193, 379, 383
Cfr10I RCCGGY 1 cut(s) 85
Csp6I GTAC 1 cut(s) 316
CviAII CATG 2 cut(s) 134, 382
CviJI RGCY 6 cut(s) 61, 85, 98, 160, 195, 215
CviKI_1 RGCY 6 cut(s) 61, 85, 98, 160, 195, 215
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 1 cut(s) 216
DpnI GATC 5 cut(s) 206, 230, 357, 400, 426
DpnII GATC 5 cut(s) 204, 228, 355, 398, 424
Eam1104I CTCTTC 2 cut(s) 183, 330
EarI CTCTTC 2 cut(s) 183, 330
Ecl136II GAGCTC 2 cut(s) 61, 98
Eco130I CCWWGG 1 cut(s) 247
Eco24I GRGCYC 2 cut(s) 63, 100
Eco53kI GAGCTC 2 cut(s) 61, 98
EcoICRI GAGCTC 2 cut(s) 61, 98
EcoT14I CCWWGG 1 cut(s) 247
EcoT38I GRGCYC 2 cut(s) 63, 100
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 2 cut(s) 137, 385
FaiI YATR 7 cut(s) 125, 135, 152, 234, 303, 383, 413
FatI CATG 2 cut(s) 133, 381
FauI CCCGC 1 cut(s) 11
FbaI TGATCA 1 cut(s) 355
FokI GGATG 3 cut(s) 27, 230, 353
FriOI GRGCYC 2 cut(s) 63, 100
FspBI CTAG 1 cut(s) 287
HapII CCGG 3 cut(s) 86, 202, 364
Hin1II CATG 2 cut(s) 137, 385
HinfI GANTC 1 cut(s) 102
HpaII CCGG 3 cut(s) 86, 202, 364
HphI GGTGA 1 cut(s) 393
Hpy188I TCNGA 4 cut(s) 58, 78, 219, 398
Hpy188III TCNNGA 3 cut(s) 106, 202, 265
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4V TGCA 1 cut(s) 240
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 2 cut(s) 137, 385
Kpn2I TCCGGA 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 5 cut(s) 204, 228, 355, 398, 424
LpnPI CCDG 5 cut(s) 99, 205, 215, 270, 377
LweI GCATC 1 cut(s) 5
MaeI CTAG 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 262
MalI GATC 5 cut(s) 206, 230, 357, 400, 426
MboI GATC 5 cut(s) 204, 228, 355, 398, 424
MboII GAAGA 3 cut(s) 91, 170, 347
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 63, 100
MluCI AATT 4 cut(s) 8, 71, 291, 311
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 1 cut(s) 331
MroI TCCGGA 1 cut(s) 201
MseI TTAA 1 cut(s) 170
MspI CCGG 3 cut(s) 86, 202, 364
MspR9I CCNGG 1 cut(s) 365
MunI CAATTG 1 cut(s) 291
NciI CCSGG 1 cut(s) 365
NdeII GATC 5 cut(s) 204, 228, 355, 398, 424
NlaIII CATG 2 cut(s) 137, 385
NmuCI GTSAC 1 cut(s) 262
NspI RCATGY 1 cut(s) 385
PaeI GCATGC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 102
Psp124BI GAGCTC 2 cut(s) 63, 100
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SacI GAGCTC 2 cut(s) 63, 100
SaqAI TTAA 1 cut(s) 170
Sau3AI GATC 5 cut(s) 204, 228, 355, 398, 424
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 2 cut(s) 63, 100
SetI ASST 4 cut(s) 63, 100, 162, 259
SfaNI GCATC 1 cut(s) 5
SphI GCATGC 1 cut(s) 385
Sse9I AATT 4 cut(s) 8, 71, 291, 311
SsiI CCGC 1 cut(s) 18
SspMI CTAG 1 cut(s) 287
SstI GAGCTC 2 cut(s) 63, 100
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 405
TaqI TCGA 7 cut(s) 6, 27, 94, 100, 105, 162, 306
TasI AATT 4 cut(s) 8, 71, 291, 311
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TseFI GTSAC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 170, 175, 324
TspGWI ACGGA 1 cut(s) 406
XapI RAATTY 1 cut(s) 71
XceI RCATGY 1 cut(s) 385
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.