Rh5DG131500

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018562
Physical Location & Seq
Reverse (-)
12889758 .. 12893549
3792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG131500.1

Sequence Viewer

Length: 327 bp
ATGGAAATTCCAATAGAAGAGATCTTTTTGGCTCAGAAAGTGATGATCTATAAGTGCAACATCCAAGGAAAACATGTTGTCACGACAATACAGATGTTGGAACTAGCAATTGTTGAATATGTCGATGAATTGTACAAGTTCTACAAACTTGAAGAGGATAACTACCGAGTTGGAGATTACTTGGACATTGATCAAGTCTGGGTTGAATGGGCAAGCATGCTGTCAAGTTTCAGATCACTAGTGTTGTATGTCTGTGGTAGATCAGTAGTTAGGAATCATTTTGTGCATATAGCTAGAACTCACTTTGGTGGCTGTAACGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

108

Amino Acids

12.71

Weight (kDa)

5.24

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 35 3.2e-09 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018562)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0353501
rosa_laevigata RLG00000012063
rosa_roxburghii Rroxscaffold_4G00325960 Rroxscaffold_5G00378580 Rroxscaffold_6G00410900
rosa_rugosa Rorug06G0205700
rosa_samantha Rh4CG126400 Rh5DG131500 Rh6CG219400 Rh6DG315700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 1 cut(s) 134
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 3 cut(s) 116, 152, 206
AhlI ACTAGT 1 cut(s) 238
AleI CACNNNNGTG 1 cut(s) 306
AluBI AGCT 1 cut(s) 293
AluI AGCT 1 cut(s) 293
ApoI RAATTY 1 cut(s) 6
BclI TGATCA 1 cut(s) 190
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 3 cut(s) 104, 239, 294
BglII AGATCT 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 47
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 5 cut(s) 21, 45, 190, 233, 260
BspCNI CTCAG 1 cut(s) 46
BspHI TCATGA 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 132
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 5 cut(s) 21, 45, 190, 233, 260
BssT1I CCWWGG 1 cut(s) 64
Bst6I CTCTTC 2 cut(s) 12, 147
BstAUI TGTACA 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 214, 218
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 60
BstKTI GATC 5 cut(s) 24, 48, 193, 236, 263
BstMBI GATC 5 cut(s) 21, 45, 190, 233, 260
BstNSI RCATGY 2 cut(s) 77, 220
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BtsCI GGATG 1 cut(s) 60
Cac8I GCNNGC 2 cut(s) 214, 218
CciI TCATGA 1 cut(s) 323
Csp6I GTAC 1 cut(s) 133
CviAII CATG 3 cut(s) 74, 217, 324
CviJI RGCY 3 cut(s) 32, 293, 312
CviKI_1 RGCY 3 cut(s) 32, 293, 312
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 33
DpnI GATC 5 cut(s) 23, 47, 192, 235, 262
DpnII GATC 5 cut(s) 21, 45, 190, 233, 260
Eam1104I CTCTTC 2 cut(s) 12, 147
EarI CTCTTC 2 cut(s) 12, 147
Eco130I CCWWGG 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 3 cut(s) 77, 220, 327
FaiI YATR 8 cut(s) 51, 75, 120, 218, 249, 288, 290, 325
FatI CATG 3 cut(s) 73, 216, 323
FbaI TGATCA 1 cut(s) 190
FokI GGATG 1 cut(s) 47
FspBI CTAG 3 cut(s) 104, 239, 294
Hin1II CATG 3 cut(s) 77, 220, 327
HinfI GANTC 2 cut(s) 274, 320
Hpy188I TCNGA 2 cut(s) 36, 233
Hpy188III TCNNGA 2 cut(s) 82, 324
HpyCH4V TGCA 2 cut(s) 57, 286
HpyF3I CTNAG 1 cut(s) 33
Hsp92II CATG 3 cut(s) 77, 220, 327
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 5 cut(s) 21, 45, 190, 233, 260
LpnPI CCDG 1 cut(s) 184
MaeI CTAG 3 cut(s) 104, 239, 294
MaeIII GTNAC 2 cut(s) 79, 314
MalI GATC 5 cut(s) 23, 47, 192, 235, 262
MboI GATC 5 cut(s) 21, 45, 190, 233, 260
MboII GAAGA 2 cut(s) 29, 164
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 21
MluCI AATT 3 cut(s) 6, 108, 128
MmeI TCCRAC 2 cut(s) 78, 151
MnlI CCTC 1 cut(s) 148
MslI CAYNNNNRTG 1 cut(s) 306
MunI CAATTG 1 cut(s) 108
NdeII GATC 5 cut(s) 21, 45, 190, 233, 260
NlaIII CATG 3 cut(s) 77, 220, 327
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 2 cut(s) 77, 220
OliI CACNNNNGTG 1 cut(s) 306
PaeI GCATGC 1 cut(s) 220
PagI TCATGA 1 cut(s) 323
PciI ACATGT 1 cut(s) 73
PfeI GAWTC 2 cut(s) 274, 320
PscI ACATGT 1 cut(s) 73
PsuI RGATCY 1 cut(s) 21
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 306
Sau3AI GATC 5 cut(s) 21, 45, 190, 233, 260
SetI ASST 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 306
SpeI ACTAGT 1 cut(s) 238
SphI GCATGC 1 cut(s) 220
Sse9I AATT 3 cut(s) 6, 108, 128
SspMI CTAG 3 cut(s) 104, 239, 294
StyI CCWWGG 1 cut(s) 64
TaqI TCGA 1 cut(s) 123
TasI AATT 3 cut(s) 6, 108, 128
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 274, 320
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 141, 312
XapI RAATTY 1 cut(s) 6
XceI RCATGY 2 cut(s) 77, 220
XspI CTAG 3 cut(s) 104, 239, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.