Rroxscaffold_6G00410900

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0018562
Physical Location & Seq
Forward (+)
33379800 .. 33386401
6602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410900.1

Sequence Viewer

Length: 645 bp
ATGGAAATTCCAATAGAAAAGATCTTTTTGGCTCAGAAAGTGATGATCTATAAGTGCAACATCCAAGGAAAACTTGTTGTCACGGCAATACAAATGTTGGAACTAGCAGTTGTTGAATATGTCGATGAATTGTACAAGTTCTACAAACTTGCAGAGTTTAGAGTTTCGGATGTCGATTTGTGGGAATCCAACCTTCGGATTTCACTTAATCCGCTATGGAATGCTTTAATCAACGGATTGATATTAGTAGTGCAGTTTGGCTTGAATCCGGAAAGAAATGGAGATGTTGATTTACGTGGGTTTTCGGGTTTCGTTTTAGAAACCAATTTAATTGTCGAATTATTGTTTACGGAATACAATTGTGTACGGGATGAGGTACCGACTAATCACTCGACGAGGATCCTTACGCTTGGATACCACTTTAGCAGATATACTATTTGGGTCTCTACGGAACGACCGAGAGAGAGGAAGAACGCGCCCCACGCACGCGAGAGAGAGAGAGGAGAAAACAGTATAGTTCGTCGGTTCTCTCTGGCCGAACGCCGCCGGCGCTCCCCCCCCGCTGCGTTCCGGTCCGGGAGAGGATTTGGGTTTGGGATGGCAGCGTTTGTCGTTGGCGGAAGCGAGTTCCATGGCCGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

214

Amino Acids

24.69

Weight (kDa)

9.17

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 34 3.8e-09 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018562)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0353501
rosa_laevigata RLG00000012063
rosa_roxburghii Rroxscaffold_4G00325960 Rroxscaffold_5G00378580 Rroxscaffold_6G00410900
rosa_rugosa Rorug06G0205700
rosa_samantha Rh4CG126400 Rh5DG131500 Rh6CG219400 Rh6DG315700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 376
AccB1I GGYRCC 1 cut(s) 376
AccII CGCG 2 cut(s) 476, 489
AccIII TCCGGA 1 cut(s) 268
AciI CCGC 4 cut(s) 212, 544, 561, 618
AclWI GGATC 2 cut(s) 394, 407
AcoI YGGCCR 2 cut(s) 534, 634
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 3 cut(s) 134, 366, 378
AfiI CCNNNNNNNGG 1 cut(s) 195
AgsI TTSAA 2 cut(s) 116, 265
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 2 cut(s) 394, 407
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 2 cut(s) 534, 634
ApeKI GCWGC 2 cut(s) 563, 602
ApoI RAATTY 1 cut(s) 6
Asp718I GGTACC 1 cut(s) 376
AspLEI GCGC 2 cut(s) 478, 552
AspS9I GGNCC 1 cut(s) 573
AsuC2I CCSGG 1 cut(s) 577
AvaII GGWCC 1 cut(s) 573
BamHI GGATCC 1 cut(s) 399
BanI GGYRCC 1 cut(s) 376
BbvI GCAGC 2 cut(s) 550, 614
BccI CCATC 1 cut(s) 592
BceAI ACGGC 1 cut(s) 99
BciVI GTATCC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 577
BcoDI GTCTC 1 cut(s) 448
BfaI CTAG 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 553
BfuI GTATCC 1 cut(s) 407
BglII AGATCT 1 cut(s) 21
BisI GCNGC 3 cut(s) 544, 564, 603
BlsI GCNGC 3 cut(s) 545, 565, 604
Bme1390I CCNGG 1 cut(s) 577
Bme18I GGWCC 1 cut(s) 573
BmgT120I GGNCC 1 cut(s) 573
BmiI GGNNCC 2 cut(s) 378, 401
BmrFI CCNGG 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 577
BsaAI YACGTR 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 2 cut(s) 64, 631
BsaWI WCCGGW 2 cut(s) 268, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse118I RCCGGY 1 cut(s) 546
BseAI TCCGGA 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 64, 631
BseGI GGATG 4 cut(s) 60, 175, 376, 603
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 47
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 2 cut(s) 550, 614
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 2 cut(s) 476, 489
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 2 cut(s) 536, 636
BshNI GGYRCC 1 cut(s) 376
BsiEI CGRYCG 1 cut(s) 458
BsiSI CCGG 5 cut(s) 269, 547, 571, 576, 637
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 448
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 2 cut(s) 536, 636
Bso31I GGTCTC 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 268
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 3 cut(s) 21, 45, 399
Bsp19I CCATGG 1 cut(s) 631
BspACI CCGC 4 cut(s) 212, 544, 561, 618
BspANI GGCC 2 cut(s) 536, 636
BspCNI CTCAG 1 cut(s) 46
BspEI TCCGGA 1 cut(s) 268
BspFNI CGCG 2 cut(s) 476, 489
BspLI GGNNCC 2 cut(s) 378, 401
BspPI GGATC 2 cut(s) 394, 407
BspT107I GGYRCC 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 64, 631
BssMI GATC 3 cut(s) 21, 45, 399
BssT1I CCWWGG 2 cut(s) 64, 631
Bst4CI ACNGT 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 487, 548
BstDEI CTNAG 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 631
BstF5I GGATG 4 cut(s) 60, 175, 376, 603
BstFNI CGCG 2 cut(s) 476, 489
BstH2I RGCGCY 1 cut(s) 553
BstHHI GCGC 2 cut(s) 478, 552
BstKTI GATC 3 cut(s) 24, 48, 402
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 3 cut(s) 21, 45, 399
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 2 cut(s) 482, 549
BstSCI CCNGG 1 cut(s) 575
BstUI CGCG 2 cut(s) 476, 489
BstV1I GCAGC 2 cut(s) 550, 614
BstX2I RGATCY 2 cut(s) 21, 399
BstYI RGATCY 2 cut(s) 21, 399
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 2 cut(s) 536, 636
BtgI CCRYGG 1 cut(s) 631
BtsCI GGATG 4 cut(s) 60, 175, 376, 603
Cac8I GCNNGC 2 cut(s) 487, 548
CfoI GCGC 2 cut(s) 478, 552
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 1 cut(s) 573
CpoI CGGWCCG 1 cut(s) 573
Csp6I GTAC 3 cut(s) 133, 365, 377
CspI CGGWCCG 1 cut(s) 573
CviAII CATG 1 cut(s) 632
CviJI RGCY 4 cut(s) 32, 261, 536, 636
CviKI_1 RGCY 4 cut(s) 32, 261, 536, 636
CviQI GTAC 3 cut(s) 133, 365, 377
DdeI CTNAG 1 cut(s) 33
DpnI GATC 3 cut(s) 23, 47, 401
DpnII GATC 3 cut(s) 21, 45, 399
EaeI YGGCCR 2 cut(s) 534, 634
EciI GGCGGA 1 cut(s) 633
Eco130I CCWWGG 2 cut(s) 64, 631
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 573
EcoT14I CCWWGG 2 cut(s) 64, 631
ErhI CCWWGG 2 cut(s) 64, 631
FaeI CATG 1 cut(s) 635
FaiI YATR 6 cut(s) 51, 120, 217, 432, 515, 633
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FatI CATG 1 cut(s) 631
FauI CCCGC 1 cut(s) 568
Fnu4HI GCNGC 3 cut(s) 544, 564, 603
FokI GGATG 4 cut(s) 47, 182, 383, 610
Fsp4HI GCNGC 3 cut(s) 544, 564, 603
FspBI CTAG 1 cut(s) 104
GlaI GCGC 2 cut(s) 477, 551
GluI GCNGC 3 cut(s) 544, 564, 603
HaeII RGCGCY 1 cut(s) 553
HaeIII GGCC 2 cut(s) 536, 636
HapII CCGG 5 cut(s) 269, 547, 571, 576, 637
HhaI GCGC 2 cut(s) 478, 552
Hin1II CATG 1 cut(s) 635
Hin6I GCGC 2 cut(s) 476, 550
HinP1I GCGC 2 cut(s) 476, 550
HinfI GANTC 2 cut(s) 185, 265
HpaII CCGG 5 cut(s) 269, 547, 571, 576, 637
Hpy166II GTNNAC 2 cut(s) 348, 365
Hpy188I TCNGA 3 cut(s) 36, 169, 198
Hpy188III TCNNGA 1 cut(s) 269
Hpy8I GTNNAC 2 cut(s) 348, 365
Hpy99I CGWCG 2 cut(s) 397, 525
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 512
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 3 cut(s) 57, 152, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 549
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 1 cut(s) 635
HspAI GCGC 2 cut(s) 476, 550
Kpn2I TCCGGA 1 cut(s) 268
KpnI GGTACC 1 cut(s) 380
KroI GCCGGC 1 cut(s) 546
KroNI GCCGGC 1 cut(s) 548
Kzo9I GATC 3 cut(s) 21, 45, 399
LmnI GCTCC 1 cut(s) 557
LpnPI CCDG 5 cut(s) 282, 518, 560, 584, 589
Lsp1109I GCAGC 2 cut(s) 550, 614
MaeI CTAG 1 cut(s) 104
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 1 cut(s) 79
MalI GATC 3 cut(s) 23, 47, 401
MboI GATC 3 cut(s) 21, 45, 399
MboII GAAGA 1 cut(s) 481
MfeI CAATTG 1 cut(s) 358
MflI RGATCY 2 cut(s) 21, 399
MluCI AATT 6 cut(s) 6, 128, 325, 330, 338, 358
MmeI TCCRAC 2 cut(s) 78, 213
MnlI CCTC 5 cut(s) 367, 390, 459, 494, 575
MreI CGCCGGCG 1 cut(s) 546
MroI TCCGGA 1 cut(s) 268
MroNI GCCGGC 1 cut(s) 546
MseI TTAA 3 cut(s) 207, 227, 329
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 5 cut(s) 269, 547, 571, 576, 637
MspR9I CCNGG 1 cut(s) 577
MunI CAATTG 1 cut(s) 358
Mva1269I GAATGC 1 cut(s) 226
MvnI CGCG 2 cut(s) 476, 489
MwoI GCNNNNNNNGC 2 cut(s) 482, 549
NaeI GCCGGC 1 cut(s) 548
NciI CCSGG 1 cut(s) 577
NcoI CCATGG 1 cut(s) 631
NdeII GATC 3 cut(s) 21, 45, 399
NgoMIV GCCGGC 1 cut(s) 546
NlaIII CATG 1 cut(s) 635
NlaIV GGNNCC 2 cut(s) 378, 401
NmuCI GTSAC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 455
PctI GAATGC 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 548
PfeI GAWTC 2 cut(s) 185, 265
PfoI TCCNGGA 1 cut(s) 575
PkrI GCNGC 3 cut(s) 545, 565, 604
Ppu21I YACGTR 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 378, 401
PspPI GGNCC 1 cut(s) 573
PsuI RGATCY 2 cut(s) 21, 399
RsaI GTAC 3 cut(s) 134, 366, 378
RsaNI GTAC 3 cut(s) 133, 365, 377
Rsr2I CGGWCCG 1 cut(s) 573
RsrII CGGWCCG 1 cut(s) 573
SaqAI TTAA 3 cut(s) 207, 227, 329
SatI GCNGC 3 cut(s) 544, 564, 603
Sau3AI GATC 3 cut(s) 21, 45, 399
Sau96I GGNCC 1 cut(s) 573
ScrFI CCNGG 1 cut(s) 577
SetI ASST 3 cut(s) 195, 298, 378
SgrAI CRCCGGYG 1 cut(s) 546
SinI GGWCC 1 cut(s) 573
Sse9I AATT 6 cut(s) 6, 128, 325, 330, 338, 358
SsiI CCGC 4 cut(s) 212, 544, 561, 618
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 575
StyI CCWWGG 2 cut(s) 64, 631
TaaI ACNGT 1 cut(s) 512
TaiI ACGT 1 cut(s) 298
TaqI TCGA 4 cut(s) 123, 174, 336, 392
TaqII GACCGA 1 cut(s) 472
TasI AATT 6 cut(s) 6, 128, 325, 330, 338, 358
TatI WGTACW 1 cut(s) 132
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 2 cut(s) 185, 265
Tru1I TTAA 3 cut(s) 207, 227, 329
Tru9I TTAA 3 cut(s) 207, 227, 329
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 2 cut(s) 563, 602
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 141
TspGWI ACGGA 3 cut(s) 249, 365, 464
VpaK11BI GGWCC 1 cut(s) 573
XapI RAATTY 1 cut(s) 6
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.