Rroxscaffold_4G00331070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007515
Physical Location & Seq
Reverse (-)
64851565 .. 64852262
698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331070.1

Sequence Viewer

Length: 567 bp
ATGATGTCGTCAACCTCGGTCCTCCCCGATTCATGGAGTGAAATTGTTGGTTACCGGAGGAGGTTGTTGATAATTGGAGTGTTTGGAAATGGCGACTTATACCGAGATAGGTCAACACTCTCGGGATATATCAAGCTCGTTATTTTGGATCATGGTTCGATATCCTTTGGAAGATTTGCTGTCGAGACGCTTGCTTGGGAAAAATGGTCTGTTTTTCGGAAAAATCGATGTCGGGAGGAGCTCGAAATGTACAAGTCGAATGGATTGGTAGCCCAAAAGAGGGCTTACTTTGAGGAGTATTATAAGAGAGTTAGAGCTCTCAAGGCATTGCAATCCGAGCATCAAGAAACAGCCCAAGTTGATGCTTGCCCACATGTGCGGATGAATTCCATGCAATTAGAAAATGACAATTCTAGTAATGATATTTGTGTGCCTTTACTTGAAAAAGGAGCTGCAGTTTACAGTTCAAAGCTGGTTTTGAATGGGATTGTTACTCGAAGCCGGAATTTGAAAGACATCGCATTTTCACCGATCAAGTTAAGAAAACCCGGTCTACCATATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.47

Weight (kDa)

9.46

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccI GTMKAC 1 cut(s) 553
AciI CCGC 1 cut(s) 379
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 2 cut(s) 385, 505
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 3 cut(s) 33, 279, 280
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 4 cut(s) 443, 468, 481, 511
AluBI AGCT 5 cut(s) 136, 241, 317, 452, 472
AluI AGCT 5 cut(s) 136, 241, 317, 452, 472
Alw21I GWGCWC 2 cut(s) 243, 319
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 156
Ama87I CYCGRG 1 cut(s) 121
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 2 cut(s) 385, 505
AspS9I GGNCC 1 cut(s) 19
AsuC2I CCSGG 1 cut(s) 549
AsuHPI GGTGA 1 cut(s) 519
AvaI CYCGRG 1 cut(s) 121
AvaII GGWCC 1 cut(s) 19
BanII GRGCYC 2 cut(s) 243, 319
Bbv12I GWGCWC 2 cut(s) 243, 319
BbvI GCAGC 1 cut(s) 439
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 453
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 549
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 349, 352
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 226
BsaJI CCNNGG 1 cut(s) 15
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 279, 280
Bse3DI GCAATG 1 cut(s) 326
BseCI ATCGAT 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 3 cut(s) 33, 279, 280
BseMI GCAATG 1 cut(s) 326
BseRI GAGGAG 3 cut(s) 73, 251, 308
BseXI GCAGC 1 cut(s) 439
BshVI ATCGAT 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 243, 319
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 3 cut(s) 55, 502, 549
BslI CCNNNNNNNGG 3 cut(s) 33, 279, 280
BsmAI GTCTC 1 cut(s) 179
BsmBI CGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 243, 319
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 2 cut(s) 148, 531
BspACI CCGC 1 cut(s) 379
BspDI ATCGAT 1 cut(s) 226
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 249
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 2 cut(s) 148, 531
Bst4CI ACNGT 1 cut(s) 464
BstAUI TGTACA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 192, 367
BstEII GGTNACC 1 cut(s) 50
BstF5I GGATG 1 cut(s) 387
BstKTI GATC 2 cut(s) 151, 534
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 2 cut(s) 148, 531
BstMWI GCNNNNNNNGC 2 cut(s) 323, 337
BstNSI RCATGY 1 cut(s) 377
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 547
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 226
BsuTUI ATCGAT 1 cut(s) 226
BtgZI GCGATG 1 cut(s) 502
BtsCI GGATG 1 cut(s) 387
Cac8I GCNNGC 2 cut(s) 192, 367
Cfr13I GGNCC 1 cut(s) 19
ClaI ATCGAT 1 cut(s) 226
CseI GACGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 250
CviAII CATG 4 cut(s) 33, 152, 374, 391
CviJI RGCY 9 cut(s) 136, 241, 272, 284, 317, 353, 452, 472, 501
CviKI_1 RGCY 9 cut(s) 136, 241, 272, 284, 317, 353, 452, 472, 501
CviQI GTAC 1 cut(s) 250
DpnI GATC 2 cut(s) 150, 533
DpnII GATC 2 cut(s) 148, 531
Ecl136II GAGCTC 2 cut(s) 241, 317
Eco24I GRGCYC 2 cut(s) 243, 319
Eco32I GATATC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 2 cut(s) 241, 317
Eco88I CYCGRG 1 cut(s) 121
Eco91I GGTNACC 1 cut(s) 50
EcoICRI GAGCTC 2 cut(s) 241, 317
EcoO65I GGTNACC 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 385
EcoRV GATATC 1 cut(s) 162
EcoT38I GRGCYC 2 cut(s) 243, 319
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 4 cut(s) 36, 155, 377, 394
FaiI YATR 9 cut(s) 34, 100, 129, 153, 303, 375, 392, 559, 561
FatI CATG 4 cut(s) 32, 151, 373, 390
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 1 cut(s) 553
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 1 cut(s) 394
FriOI GRGCYC 2 cut(s) 243, 319
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 1 cut(s) 414
GluI GCNGC 1 cut(s) 453
HapII CCGG 3 cut(s) 55, 502, 549
HgaI GACGC 1 cut(s) 196
Hin1II CATG 4 cut(s) 36, 155, 377, 394
HincII GTYRAC 2 cut(s) 12, 114
HindII GTYRAC 2 cut(s) 12, 114
HinfI GANTC 1 cut(s) 29
HpaII CCGG 3 cut(s) 55, 502, 549
HphI GGTGA 1 cut(s) 519
Hpy166II GTNNAC 4 cut(s) 12, 114, 460, 554
Hpy188I TCNGA 2 cut(s) 219, 337
Hpy188III TCNNGA 4 cut(s) 123, 184, 233, 344
Hpy8I GTNNAC 4 cut(s) 12, 114, 460, 554
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4V TGCA 3 cut(s) 331, 394, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 337
Hsp92II CATG 4 cut(s) 36, 155, 377, 394
Kzo9I GATC 2 cut(s) 148, 531
LmnI GCTCC 2 cut(s) 238, 449
LpnPI CCDG 4 cut(s) 68, 458, 515, 562
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 2 cut(s) 349, 352
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 2 cut(s) 50, 490
MalI GATC 2 cut(s) 150, 533
MboI GATC 2 cut(s) 148, 531
MboII GAAGA 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 243, 319
MluCI AATT 6 cut(s) 42, 72, 385, 395, 409, 505
MnlI CCTC 7 cut(s) 25, 32, 51, 54, 229, 273, 286
MseI TTAA 1 cut(s) 539
MspI CCGG 3 cut(s) 55, 502, 549
MspR9I CCNGG 1 cut(s) 549
MwoI GCNNNNNNNGC 2 cut(s) 323, 337
NciI CCSGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 559
NdeII GATC 2 cut(s) 148, 531
NlaIII CATG 4 cut(s) 36, 155, 377, 394
NspI RCATGY 1 cut(s) 377
PciI ACATGT 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 1 cut(s) 454
PscI ACATGT 1 cut(s) 373
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 2 cut(s) 243, 319
PspEI GGTNACC 1 cut(s) 50
PspPI GGNCC 1 cut(s) 19
PstI CTGCAG 1 cut(s) 457
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SacI GAGCTC 2 cut(s) 243, 319
SaqAI TTAA 1 cut(s) 539
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 2 cut(s) 148, 531
Sau96I GGNCC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 549
SduI GDGCHC 2 cut(s) 243, 319
SetI ASST 8 cut(s) 17, 65, 113, 138, 243, 319, 454, 474
SfaNI GCATC 2 cut(s) 349, 352
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 1 cut(s) 19
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 6 cut(s) 42, 72, 385, 395, 409, 505
SsiI CCGC 1 cut(s) 379
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 2 cut(s) 243, 319
StyD4I CCNGG 1 cut(s) 547
TaaI ACNGT 1 cut(s) 464
TaqI TCGA 6 cut(s) 158, 183, 226, 243, 257, 496
TaqII GACCGA 1 cut(s) 7
TasI AATT 6 cut(s) 42, 72, 385, 395, 409, 505
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 1 cut(s) 539
Tru9I TTAA 1 cut(s) 539
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 2 cut(s) 21, 398
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 2 cut(s) 385, 505
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 553
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.