Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0011934

Physical Location & Seq

Genomic Coordinates Reverse (-)

65232189 .. 65244831

Gene Structure 12643 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00331320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4632 bp

ATGAAGAAACGCCGCGTTCCGCAGGTCCTTTGGCGCCTCTTCCACCACCGCGCCCGCACTCTGGCCCACACTCTCACCTCCTTACTCCCTCCTCATTCCTCCTCTCCAAACGCCCAATGTCGTTTCTGCAAGGGCCGCCGTTGCCTCAGCTGCTGCACCGGCCCCGACGCCGTGTCGTTTCTCCTTCGACCCGACGATCCCTCCGATTACCGCTCCCTCCTCGACAAGTGCTTCGTCGTCGTCTCCGAAAACGCCCCCGCCATCACTCGCTTCTCCCCTGAATCTCACTGGTCCCAAATTGAGATCGTTAGAGCAGTCATCGAAACGACGATGCTGGAGCAATCTATGTCTTCCAATGTGATATGCAATGGTTATGACAAGCGTGACAAGTCAAGTGCCATTGTGGAACTTCTGACTTCTTCGGCTTGGTGTCGTCTCTTAGAAAGGGTTGGCGATGGCATTATGGTTTATTTACTAAAGAATGCGTCAATATTTTTGAGAATTCCGGATAAGAAACACCAGCAGGTGACAGGTCCTCCCATTAGTGACTTATGTCGTAAGAAGTTCTATTGCACACCGAATTCACAACGGCAACCGTCATCCCTTGCTCCATGTGGACCGCGGAAAAAGAGGAAAAGAGATGATAATGTTCACTCAATGTTAAAGAAAAAGCAGTTCATGAGTTCAGTCAGCATTGGAGAAAGCTCGAGTTCTGTTACCTGCAATGGCTGCTGTAACAATAGTGAAAATTATAGGCTTATGCATAATCATATAAGTTTCTCTGAAGCATCTACATCAGCAACCAGCACGCTTACCAATGATTATGAAGGGAATTTAAATCAGGAACTTACAGAGAGTGTGGAATCTCTAAGAAAGCGTTCAAGACCATTTCGTTGGCAGCGCTGCAGGAAACGCAGACAGTTATCTTTGCAAGAAACTGGTGTTAAGGATCTTTTCAGAACAACTGTCGGTGATAAACAATGGTTACCTGGCAGGCTTTCGTCTTCCTTGAAACCGAGCTTAGGTCGACATTATGAACAGTGCAATTGTTTGGGGTTCCAAGTTGCCCAAAAGGTTTCCAATGGTGCTCAAATAGACAGGAAATCCTTGTTCTTCAATTTGGAATCTTCTTCATCAGTCTTTCCTAAAAAGCATGTGCTGAATTCTGTAAAGCCTGATTCTGTTGGTTCAGAGTTTCTTGTCCAGAGTATTTTTGGCATGTATGACATAGAAAGTGCACTGTCAAAGACATGTCCCAATGGCAGTGGTCTATGTCTCATGGGATCTGCATGCCTATATCACTCGCTTGTTAAGTTGCTTAAAATTCTTATACGTAGAGCACGGTATGGCCATCATGTGAGATTACTGGAAAAGCATTGTGTTGTTAGAACTGTAAATCCTAATACAATCAAAAGCGGTGGTTGCATCTCTGAGGGAGAAAAGTGGCAGTACAATGTACTCAAGAAATCTCAGTGTTGTACTGCGGACCATTGCAATAAATCTCCAGAAGTTAGTGAATCTTTCTCTGAGGAAATTAAGTCTTACTGCTTGAACAGTCAGGTGGAGTCATTTATATGGGCTGTTTGTAGGAGTATTGTTCCTCCTGAGTTGCTAGGAACTCCTTCTAACTGGAGAATTTTGAGGAGAACTATTTCAAAGTTCATTCGACTTAGAAGATTTGAAAGGTTTTCCTTAAAACAATGTATGCATAAATTGAAAACGTCTAGGTTCCCGTTTCTATCAAATAAACATTATTTCTGCTGCATGAATAATGAGGCACCGAAACATGCAGATGGAAAAGGTGTAGACATTCAAAAGGGATCCGGTACATTGAATGATGCTGTACATATTGTTAAAGTAAAACTCTTAGAAAGTTGGATTTATTGGTTCTTTTCATCTGTAGTTGTGCCATTGTTGCAAGCCAATTTCTATGTCACTGAAACTGAGCATGGGAAGCAAGATGTATATTACTACCGGAAGTCAGTTTGGGAGAAGGTGAAGAATAAGACCATCGTCACTTGCATGGATCAGAGCTATCAATATTTGGATGATGCGACAACTAGAAGAATTATAAGGAAACGATCATTTGGGTTTTCAAAGCTCCGGATTTGTCCAAAAGAGTATGGAGTGAGGTTGCTAGCAAATTTAAAAGCACCATCAAAATTGCCAGGGAAAGAGTCCTGTTTGGCAGATCAGTCTAGTGGAATATTTAGAGGAAAGAAACTGCATAGGAGAAAGGTTAGATTTGAGCATTTCAAGTCCGTGAATCGTGTTCTTCGTGATACACATGTTGTTCTAAAAGCTATGCAGCTGAAAGAGACAGGGAAATTAGGATCATCGGTGTTTGATTACAATGATGTATACAGAAAGTTGTGCCCATTTCTAATTGGCATGAAGAGTGGATTCACTATGATACCTGATGTGTTTATCATTGTCTCTGATGTATCAAAAGCCTTTGATTCTGTTGACCAGGATAAGCTGCTTGGTGTGCTGAAAGATGTTATAAGAGCAGATGAATATTTTCTCCAGCAGTCTCATCATGTCATTTTCAAGAAGGGTTCATTAAGGATTCATGAGAACCGTGCATTTCTAGATCAAAATACTGGCTCAAGATTTAAGTCTAAATTACGTAGTGTCCTTGTTAATCAGGAGTGCAGCAGGTCTGTGAAGAAGAAGGAGCTATTTTTTAATCTAAATGAGCATGTGAAGCGCAATGTGCTGCAGTTGGATAAAAAGTTTTATCTGCAACGCATCGGTATTCCTCAAGGAAGCGTTTTGTCAACTTTGCTTTGCTCATTATACTATGGACATCTCGATCAGAATGTGATCTTTCCATTTCTTGACAGAACTTGGGAACCTTCTATTCCAGATTTATCAAGAGGACATGATTTTCAAGTTGCTTCTGCTGCAGAGAGTAGCAGTGAGGATAAAATATCCTCATCTTCTAGATATACTCTACTTAGATTTATTGATGATTTTCTTTTCATTTCAACCTCAAAGGTGCAGGCTGCCAGCTTCTTCTCCAGGTTGCAAAGAGGATTTCGGGATTACAACTGCTACATGAACGAGAAAAAGTTCTGTGTAAATTTTGATATTGGAAACCTGCCATCAAACAGGGTCTACAGTGGTGATGATGGCATTTTATTTATTCGATGGAGTGGCTTGCTTATCAATTCTCGCACACTTGAAGTTCAGGCAGACTATACGAAGTACTTGAAAAACCATCTAAGTTCGACTGTCACTGTCCACTACCAAGGCAAACCTGGTCGCCATTTTAAGGGAAAGTTGCGTGACTACATGAGACCTAAATGTCATCCCTTATTCTTTGATTCCCACATTAATTCAGCATCTGTTGTGAGGTTAAATATCTACCAAGCATTCTTGCTATGTGCAATGAAGTTCCATTGTTATGTTCGTGACTTATCATATATCTGCAAATTTCACATTGGATTTTACGCTTACAGTATCCAAAAATCTTTGAGGTACATGCACAGGCTCATAAAGAAAAGGATGTGTTCATTGCATACCGGCTCTAACTTCCGCCCAATTCTTCAATTGGAAAAGGGAGAAGTTGAATGGCTTGGTTTATATGCATATATCCAAGTACTGAAGAGAAAACAATCACGGGTCACATTTGGAAGCTCCCCAGCTGTTTTAGGGTTTCTCCTGAAAATGGCATTCGCTAATCGAATCCGATCGAGCCTCCCTCTCCTCTCCAAGATTCTCAAATCCGATTCACTCGCCGCCGCCAATGCCTCAGCCGCCCCCCGTGCTCTACTTTGTCCTACTCTCTCCGATTCCGAGTTGTCGAGGAACTTTTCCACTGCTCCTGGGAAGAAGGATGTGAAGGTCAAGGTGCCTTTGGCATTGTTTGGGGGATCTGGGAACTATGCCTCTGCTTTGTACATTGCTGCAGTGAAAACTAATTCCTTGGACAAGGTTGAGTCTGAGATTCTTGACTTTGTTGGGGCAATGACGAAAAGTCCTATGTTCTCTCAGTTCACAAAGGATTTCTCAGTGCCTGCAGATACTAGAAAGAAGGCAATTGAAGATATTTCTGCTCAAGCTAAATTTTCAGAAATTACAAAGAACTTCTTGAGGGGTTATTATGCTCTTGTTCTGATGATGAGAGTGGAGAGGGATACGCAGAGGGATCTGGTGGCAGATCCGAATTCATTGTTGACACTACTATTGCGTATTGTCCCTGGAAAAGGAGCCTCTGATGTTTGCAAATTTAGGACATTTCAACAAAACAAACTCTCTGCTGGAGTGCTGCCCTTACTGTTACAAGAAGTTGTAGCAGTCGTCTTGGCTGAGAATGGAAGGCTAAGGAACATTGATACCATAGCGAAGAGATTTGTGGAATTGACCATGGCACATAAGGGAGTAGTCAAAGTTATTGTGACATCTGTCATTCCCCTCCCTGCTGAAGAGGAAAAGGAATTGAAGGAGACACTGCAGGACTTAATTGGTAAAGGGAAGACGGTCAAGCTTGAACAGAAGATCGATACCAGTATTATGGGTGGGCTTGTGGTAGAGTTTCAGCAGAAGGTGTTTGACATGTCCATTAAGACTAGGGCACGCCAGTTTGAGAGGTACTTGCGGGATCCCATTCACTGGGACAACCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000333 GO:0000723 GO:0003674 GO:0003676 GO:0003677 GO:0003720 GO:0003721 GO:0003723 GO:0003824 GO:0003964 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0006139 GO:0006259 GO:0006278 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007000 GO:0007004 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009888 GO:0009987 GO:0010073 GO:0010449 GO:0010833 GO:0016043 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0019827 GO:0022622 GO:0032200 GO:0032501 GO:0032502 GO:0032991 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0035266 GO:0040007 GO:0042162 GO:0042575 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048364 GO:0048507 GO:0048589 GO:0048731 GO:0048856 GO:0050000 GO:0051179 GO:0051276 GO:0051640 GO:0051641 GO:0060249 GO:0061695 GO:0065007 GO:0065008 GO:0070034 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0097159 GO:0098727 GO:0099402 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05165 ko05166 ko05200 ko05225 ko05226 map05165 map05166 map05200 map05225 map05226

Pfam Domains

Protein Families

RVT_1 Telomerase_RBD

Protein Analysis

1543

Amino Acids

175.86

Weight (kDa)

9.69

Isoelectric Point (pI)

47.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Telomerase_RBD	PF12009	520 - 671	1.9e-35	Telomerase ribonucleoprotein complex - RNA binding domain
TERT_C	PF21399	1090 - 1208	5.1e-16	Telomerase reverse transcriptase, C-terminal extension
OSCP	PF00213	1295 - 1367	6.5e-10	ATP synthase delta (OSCP) subunit
OSCP	PF00213	1436 - 1533	6.2e-23	ATP synthase delta (OSCP) subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011934)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16850 AT5G16850
fragaria_vesca	FvH4_7g01000 FvH4_7g01000
malus_domestica	MD07G1010300.v1.1
prunus_persica	Prupe.2G012600_v2.0.a1 Prupe.2G012600_v2.0.a1 Prupe.2G012600_v2.0.a1 Prupe.2G012600_v2.0.a1 Prupe.2G012600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0315901
rosa_laevigata	RLG00000030725
rosa_roxburghii	Rroxscaffold_4G00331320
rosa_rugosa	Rorug01G0006900
rosa_samantha	Rh1AG017600 Rh1BG010500 Rh1CG015000 Rh1DG010600
rosa_wichuraiana	Rw1G001060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2072, 2504
AarI	CACCTGC	1 cut(s)	514
AasI	GACNNNNNNGTC	1 cut(s)	3309
Acc36I	ACCTGC	5 cut(s)	13, 514, 728, 2649, 3111
AccB1I	GGYRCC	3 cut(s)	33, 1777, 3856
AccB7I	CCANNNNNTGG	1 cut(s)	4617
AccBSI	CCGCTC	1 cut(s)	213
AccI	GTMKAC	4 cut(s)	1027, 1806, 2361, 3120
AccII	CGCG	3 cut(s)	15, 51, 622
AccIII	TCCGGA	2 cut(s)	505, 2103
AcoI	YGGCCR	1 cut(s)	1348
AcuI	CTGAAG	3 cut(s)	804, 3629, 4449
AcyI	GRCGYC	2 cut(s)	34, 168
AfeI	AGCGCT	1 cut(s)	902
AfiI	CCNNNNNNNGG	6 cut(s)	61, 1022, 3277, 3673, 4211, 4617
AflIII	ACRYGT	3 cut(s)	1250, 2287, 4560
AhdI	GACNNNNNGTC	2 cut(s)	172, 3981
AjnI	CCWGG	7 cut(s)	988, 2167, 2469, 3023, 3262, 3829, 4204
AloI	GAACNNNNNNTCC	2 cut(s)	1381, 1413
Alw21I	GWGCWC	4 cut(s)	1090, 1240, 1342, 3775
Alw26I	GTCTC	8 cut(s)	247, 440, 1280, 2312, 2440, 2538, 3295, 4445
Alw44I	GTGCAC	1 cut(s)	1236
AlwNI	CAGNNNCTG	3 cut(s)	153, 3350, 4022
Ama87I	CYCGRG	1 cut(s)	706
Aor13HI	TCCGGA	2 cut(s)	505, 2103
Aor51HI	AGCGCT	1 cut(s)	902
AoxI	GGCC	4 cut(s)	63, 133, 160, 1348
ApaLI	GTGCAC	1 cut(s)	1236
ArsI	GACNNNNNNTTYG	2 cut(s)	215, 247
AseI	ATTAAT	1 cut(s)	3339
Asp700I	GAANNNNTTC	5 cut(s)	877, 1621, 1651, 2520, 3074
AspLEI	GCGC	4 cut(s)	36, 53, 903, 2712
AspS9I	GGNCC	8 cut(s)	25, 64, 133, 161, 291, 533, 617, 1486
AsuHPI	GGTGA	5 cut(s)	67, 538, 983, 2008, 3140
AsuNHI	GCTAGC	1 cut(s)	2137
AvaI	CYCGRG	1 cut(s)	706
AvaII	GGWCC	5 cut(s)	25, 291, 533, 617, 1486
BaeGI	GKGCMC	3 cut(s)	1240, 2378, 4582
BaeI	ACNNNNGTAYC	2 cut(s)	2274, 2307
BalI	TGGCCA	1 cut(s)	1350
BamHI	GGATCC	2 cut(s)	1820, 4606
BanI	GGYRCC	3 cut(s)	33, 1777, 3856
BbsI	GAAGAC	3 cut(s)	342, 996, 4487
Bbv12I	GWGCWC	4 cut(s)	1090, 1240, 1342, 3775
BbvCI	CCTCAGC	2 cut(s)	146, 3757
BceAI	ACGGC	3 cut(s)	123, 155, 605
BciT130I	CCWGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
BciVI	GTATCC	2 cut(s)	3476, 4135
BcoDI	GTCTC	8 cut(s)	247, 440, 1280, 2312, 2440, 2538, 3295, 4445
BfaI	CTAG	9 cut(s)	1613, 1725, 2061, 2138, 2199, 2591, 2946, 4032, 4575
BfmI	CTRYAG	8 cut(s)	904, 1899, 2720, 2907, 3121, 3912, 4023, 4457
BfoI	RGCGCY	2 cut(s)	37, 904
BfuAI	ACCTGC	5 cut(s)	13, 514, 728, 2649, 3111
BfuI	GTATCC	2 cut(s)	3476, 4135
BmcAI	AGTACT	2 cut(s)	3212, 3606
Bme1390I	CCNGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
Bme18I	GGWCC	5 cut(s)	25, 291, 533, 617, 1486
BmeRI	GACNNNNNGTC	2 cut(s)	172, 3981
BmeT110I	CYCGRG	1 cut(s)	706
BmgT120I	GGNCC	8 cut(s)	25, 64, 133, 161, 291, 533, 617, 1486
BmrFI	CCNGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
BmrI	ACTGGG	1 cut(s)	4627
BmsI	GCATC	7 cut(s)	321, 797, 1434, 1828, 2041, 2760, 3356
BmtI	GCTAGC	1 cut(s)	2141
BmuI	ACTGGG	1 cut(s)	4627
BoxI	GACNNNNGTC	3 cut(s)	552, 2012, 4409
BpiI	GAAGAC	3 cut(s)	342, 996, 4487
BplI	GAGNNNNNCTC	2 cut(s)	3691, 3723
BpmI	CTGGAG	6 cut(s)	356, 1488, 1651, 2510, 3007, 4287
Bpu10I	CCTNAGC	4 cut(s)	146, 1021, 3757, 4328
BpuEI	CTTGAG	5 cut(s)	1445, 2593, 2748, 4047, 4117
Bsa29I	ATCGAT	1 cut(s)	4506
BsaAI	YACGTR	2 cut(s)	1334, 2630
BsaHI	GRCGYC	2 cut(s)	34, 168
BsaI	GGTCTC	1 cut(s)	3295
BsaJI	CCNNGG	7 cut(s)	620, 2168, 3253, 3830, 3930, 4204, 4371
BsaWI	WCCGGW	4 cut(s)	505, 1823, 1974, 2103
BsaXI	ACNNNNNCTCC	4 cut(s)	185, 215, 520, 550
Bsc4I	CCNNNNNNNGG	6 cut(s)	61, 1022, 3277, 3673, 4211, 4617
Bse118I	RCCGGY	2 cut(s)	158, 3527
Bse1I	ACTGG	8 cut(s)	293, 943, 1371, 1634, 2608, 4512, 4585, 4622
Bse3DI	GCAATG	8 cut(s)	373, 730, 1489, 2719, 3399, 3518, 3906, 3978
BseAI	TCCGGA	2 cut(s)	505, 2103
BseBI	CCWGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
BseCI	ATCGAT	1 cut(s)	4506
BseDI	CCNNGG	7 cut(s)	620, 2168, 3253, 3830, 3930, 4204, 4371
BseGI	GGATG	5 cut(s)	599, 2053, 3313, 3516, 3847
BseLI	CCNNNNNNNGG	6 cut(s)	61, 1022, 3277, 3673, 4211, 4617
BseMI	GCAATG	8 cut(s)	373, 730, 1489, 2719, 3399, 3518, 3906, 3978
BseNI	ACTGG	8 cut(s)	293, 943, 1371, 1634, 2608, 4512, 4585, 4622
BseRI	GAGGAG	5 cut(s)	81, 91, 209, 1657, 3701
BseSI	GKGCMC	3 cut(s)	1240, 2378, 4582
BseYI	CCCAGC	1 cut(s)	3646
BsgI	GTGCAG	3 cut(s)	139, 2674, 3023
Bsh1236I	CGCG	3 cut(s)	15, 51, 622
Bsh1285I	CGRYCG	1 cut(s)	3698
BshFI	GGCC	4 cut(s)	65, 135, 162, 1350
BshNI	GGYRCC	3 cut(s)	33, 1777, 3856
BshVI	ATCGAT	1 cut(s)	4506
BsiEI	CGRYCG	1 cut(s)	3698
BsiHKAI	GWGCWC	4 cut(s)	1090, 1240, 1342, 3775
BsiHKCI	CYCGRG	1 cut(s)	706
BsiSI	CCGG	6 cut(s)	159, 506, 1824, 1975, 2104, 3528
BslFI	GGGAC	3 cut(s)	277, 1239, 4187
BslI	CCNNNNNNNGG	6 cut(s)	61, 1022, 3277, 3673, 4211, 4617
BsmAI	GTCTC	8 cut(s)	247, 440, 1280, 2312, 2440, 2538, 3295, 4445
BsmBI	CGTCTC	2 cut(s)	247, 440
BsmFI	GGGAC	3 cut(s)	277, 1239, 4187
BsmI	GAATGC	3 cut(s)	487, 3377, 3677
BsnI	GGCC	4 cut(s)	65, 135, 162, 1350
Bso31I	GGTCTC	1 cut(s)	3295
BsoBI	CYCGRG	1 cut(s)	706
Bsp1286I	GDGCHC	6 cut(s)	1090, 1240, 1342, 2378, 3775, 4582
Bsp13I	TCCGGA	2 cut(s)	505, 2103
Bsp1407I	TGTACA	2 cut(s)	1843, 3903
Bsp19I	CCATGG	1 cut(s)	4371
BspANI	GGCC	4 cut(s)	65, 135, 162, 1350
BspDI	ATCGAT	1 cut(s)	4506
BspEI	TCCGGA	2 cut(s)	505, 2103
BspFNI	CGCG	3 cut(s)	15, 51, 622
BspHI	TCATGA	2 cut(s)	678, 2572
BspMAI	CTGCAG	6 cut(s)	908, 2724, 2911, 3916, 4027, 4461
BspMI	ACCTGC	5 cut(s)	13, 514, 728, 2649, 3111
BspOI	GCTAGC	1 cut(s)	2141
BspT107I	GGYRCC	3 cut(s)	33, 1777, 3856
BspTNI	GGTCTC	1 cut(s)	3295
BsrBI	CCGCTC	1 cut(s)	213
BsrDI	GCAATG	8 cut(s)	373, 730, 1489, 2719, 3399, 3518, 3906, 3978
BsrFI	RCCGGY	2 cut(s)	158, 3527
BsrGI	TGTACA	2 cut(s)	1843, 3903
BsrI	ACTGG	8 cut(s)	293, 943, 1371, 1634, 2608, 4512, 4585, 4622
BssAI	RCCGGY	2 cut(s)	158, 3527
BssECI	CCNNGG	7 cut(s)	620, 2168, 3253, 3830, 3930, 4204, 4371
BssNAI	GTATAC	1 cut(s)	2362
BssNI	GRCGYC	2 cut(s)	34, 168
BssT1I	CCWWGG	3 cut(s)	3253, 3930, 4371
Bst1107I	GTATAC	1 cut(s)	2362
Bst2UI	CCWGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
Bst6I	CTCTTC	5 cut(s)	44, 2390, 3605, 4346, 4425
BstACI	GRCGYC	2 cut(s)	34, 168
BstAPI	GCANNNNNTGC	1 cut(s)	729
BstAUI	TGTACA	2 cut(s)	1843, 3903
BstBAI	YACGTR	2 cut(s)	1334, 2630
BstDSI	CCRYGG	2 cut(s)	620, 4371
BstEII	GGTNACC	1 cut(s)	984
BstENI	CCTNNNNNAGG	1 cut(s)	4209
BstF5I	GGATG	5 cut(s)	599, 2053, 3313, 3516, 3847
BstFNI	CGCG	3 cut(s)	15, 51, 622
BstH2I	RGCGCY	2 cut(s)	37, 904
BstHHI	GCGC	4 cut(s)	36, 53, 903, 2712
BstMAI	GTCTC	8 cut(s)	247, 440, 1280, 2312, 2440, 2538, 3295, 4445
BstMCI	CGRYCG	1 cut(s)	3698
BstNI	CCWGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
BstNSI	RCATGY	9 cut(s)	1157, 1222, 1254, 1293, 1790, 2291, 2705, 3490, 4564
BstPAI	GACNNNNGTC	3 cut(s)	552, 2012, 4409
BstPI	GGTNACC	1 cut(s)	984
BstSCI	CCNGG	7 cut(s)	988, 2167, 2469, 3023, 3262, 3829, 4204
BstSFI	CTRYAG	8 cut(s)	904, 1899, 2720, 2907, 3121, 3912, 4023, 4457
BstSLI	GKGCMC	3 cut(s)	1240, 2378, 4582
BstSNI	TACGTA	2 cut(s)	1334, 2630
BstUI	CGCG	3 cut(s)	15, 51, 622
BstV2I	GAAGAC	3 cut(s)	342, 996, 4487
BstX2I	RGATCY	7 cut(s)	949, 1283, 1820, 3878, 4153, 4165, 4606
BstXI	CCANNNNNNTGG	2 cut(s)	894, 4519
BstYI	RGATCY	7 cut(s)	949, 1283, 1820, 3878, 4153, 4165, 4606
BstZ17I	GTATAC	1 cut(s)	2362
Bsu15I	ATCGAT	1 cut(s)	4506
BsuI	GTATCC	2 cut(s)	3476, 4135
BsuRI	GGCC	4 cut(s)	65, 135, 162, 1350
BsuTUI	ATCGAT	1 cut(s)	4506
BtgI	CCRYGG	2 cut(s)	620, 4371
BtgZI	GCGATG	1 cut(s)	468
BtsCI	GGATG	5 cut(s)	599, 2053, 3313, 3516, 3847
BtsI	GCAGTG	5 cut(s)	1270, 2926, 3822, 3921, 4454
BveI	ACCTGC	5 cut(s)	13, 514, 728, 2649, 3111
CaiI	CAGNNNCTG	3 cut(s)	153, 3350, 4022
CciI	TCATGA	2 cut(s)	678, 2572
CfoI	GCGC	4 cut(s)	36, 53, 903, 2712
Cfr10I	RCCGGY	2 cut(s)	158, 3527
Cfr13I	GGNCC	8 cut(s)	25, 64, 133, 161, 291, 533, 617, 1486
Cfr42I	CCGCGG	1 cut(s)	623
ClaI	ATCGAT	1 cut(s)	4506
CseI	GACGC	2 cut(s)	176, 474
CsiI	ACCWGGT	1 cut(s)	3262
CspCI	CAANNNNNGTGG	4 cut(s)	1246, 1281, 1399, 1434
DinI	GGCGCC	1 cut(s)	35
DraI	TTTAAA	2 cut(s)	837, 2148
DrdI	GACNNNNNNGTC	1 cut(s)	3309
DriI	GACNNNNNGTC	2 cut(s)	172, 3981
DseDI	GACNNNNNNGTC	1 cut(s)	3309
EaeI	YGGCCR	1 cut(s)	1348
Eam1104I	CTCTTC	5 cut(s)	44, 2390, 3605, 4346, 4425
Eam1105I	GACNNNNNGTC	2 cut(s)	172, 3981
EarI	CTCTTC	5 cut(s)	44, 2390, 3605, 4346, 4425
EciI	GGCGGA	1 cut(s)	3530
Eco105I	TACGTA	2 cut(s)	1334, 2630
Eco130I	CCWWGG	3 cut(s)	3253, 3930, 4371
Eco31I	GGTCTC	1 cut(s)	3295
Eco47I	GGWCC	5 cut(s)	25, 291, 533, 617, 1486
Eco47III	AGCGCT	1 cut(s)	902
Eco57I	CTGAAG	3 cut(s)	804, 3629, 4449
Eco88I	CYCGRG	1 cut(s)	706
Eco91I	GGTNACC	1 cut(s)	984
EcoNI	CCTNNNNNAGG	1 cut(s)	4209
EcoO109I	RGGNCCY	2 cut(s)	25, 533
EcoO65I	GGTNACC	1 cut(s)	984
EcoRI	GAATTC	4 cut(s)	501, 580, 1162, 4171
EcoRII	CCWGG	7 cut(s)	988, 2167, 2469, 3023, 3262, 3829, 4204
EcoT14I	CCWWGG	3 cut(s)	3253, 3930, 4371
EcoT22I	ATGCAT	3 cut(s)	765, 1710, 3595
EgeI	GGCGCC	1 cut(s)	35
EheI	GGCGCC	1 cut(s)	35
ErhI	CCWWGG	3 cut(s)	3253, 3930, 4371
Esp3I	CGTCTC	2 cut(s)	247, 440
FalI	AAGNNNNNCTT	2 cut(s)	4079, 4111
FaqI	GGGAC	3 cut(s)	277, 1239, 4187
FauI	CCCGC	3 cut(s)	62, 265, 4596
FblI	GTMKAC	4 cut(s)	1027, 1806, 2361, 3120
FokI	GGATG	5 cut(s)	586, 2060, 3300, 3523, 3854
FspBI	CTAG	9 cut(s)	1613, 1725, 2061, 2138, 2199, 2591, 2946, 4032, 4575
GlaI	GCGC	4 cut(s)	35, 52, 902, 2711
GsaI	CCCAGC	1 cut(s)	3650
GsuI	CTGGAG	6 cut(s)	356, 1488, 1651, 2510, 3007, 4287
HaeII	RGCGCY	2 cut(s)	37, 904
HaeIII	GGCC	4 cut(s)	65, 135, 162, 1350
HapII	CCGG	6 cut(s)	159, 506, 1824, 1975, 2104, 3528
HgaI	GACGC	2 cut(s)	176, 474
HhaI	GCGC	4 cut(s)	36, 53, 903, 2712
Hin1I	GRCGYC	2 cut(s)	34, 168
Hin6I	GCGC	4 cut(s)	34, 51, 901, 2710
HinP1I	GCGC	4 cut(s)	34, 51, 901, 2710
HincII	GTYRAC	4 cut(s)	1028, 2467, 2781, 4182
HindII	GTYRAC	4 cut(s)	1028, 2467, 2781, 4182
HindIII	AAGCTT	1 cut(s)	4490
HpaII	CCGG	6 cut(s)	159, 506, 1824, 1975, 2104, 3528
HphI	GGTGA	5 cut(s)	67, 538, 983, 2008, 3140
Hpy99I	CGWCG	5 cut(s)	170, 197, 239, 242, 331
HpyCH4IV	ACGT	3 cut(s)	1333, 1721, 2629
HpySE526I	ACGT	3 cut(s)	1333, 1721, 2629
Hsp92I	GRCGYC	2 cut(s)	34, 168
HspAI	GCGC	4 cut(s)	34, 51, 901, 2710
KasI	GGCGCC	1 cut(s)	33
Kpn2I	TCCGGA	2 cut(s)	505, 2103
KspI	CCGCGG	1 cut(s)	623
LmnI	GCTCC	8 cut(s)	218, 337, 613, 2106, 2677, 3647, 3832, 4214
LweI	GCATC	7 cut(s)	321, 797, 1434, 1828, 2041, 2760, 3356
MabI	ACCWGGT	1 cut(s)	3262
MaeI	CTAG	9 cut(s)	1613, 1725, 2061, 2138, 2199, 2591, 2946, 4032, 4575
MaeII	ACGT	3 cut(s)	1333, 1721, 2629
MbiI	CCGCTC	1 cut(s)	213
MfeI	CAATTG	3 cut(s)	1045, 3554, 4044
MflI	RGATCY	7 cut(s)	949, 1283, 1820, 3878, 4153, 4165, 4606
MhlI	GDGCHC	6 cut(s)	1090, 1240, 1342, 2378, 3775, 4582
MlsI	TGGCCA	1 cut(s)	1350
MluNI	TGGCCA	1 cut(s)	1350
Mly113I	GGCGCC	1 cut(s)	34
MlyI	GAGTC	3 cut(s)	1574, 2186, 3953
MmeI	TCCRAC	2 cut(s)	1856, 2706
Mox20I	TGGCCA	1 cut(s)	1350
Mph1103I	ATGCAT	3 cut(s)	765, 1710, 3595
MroI	TCCGGA	2 cut(s)	505, 2103
MroXI	GAANNNNTTC	5 cut(s)	877, 1621, 1651, 2520, 3074
MscI	TGGCCA	1 cut(s)	1350
MslI	CAYNNNNRTG	4 cut(s)	1573, 1791, 2021, 3408
Msp20I	TGGCCA	1 cut(s)	1350
MspA1I	CMGCKG	4 cut(s)	150, 622, 2311, 3650
MspI	CCGG	6 cut(s)	159, 506, 1824, 1975, 2104, 3528
MspR9I	CCNGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
MunI	CAATTG	3 cut(s)	1045, 3554, 4044
Mva1269I	GAATGC	3 cut(s)	487, 3377, 3677
MvaI	CCWGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
MvnI	CGCG	3 cut(s)	15, 51, 622
NarI	GGCGCC	1 cut(s)	34
NcoI	CCATGG	1 cut(s)	4371
NheI	GCTAGC	1 cut(s)	2137
NsiI	ATGCAT	3 cut(s)	765, 1710, 3595
NspI	RCATGY	9 cut(s)	1157, 1222, 1254, 1293, 1790, 2291, 2705, 3490, 4564
PaeI	GCATGC	1 cut(s)	1293
PaeR7I	CTCGAG	1 cut(s)	706
PagI	TCATGA	2 cut(s)	678, 2572
PaqCI	CACCTGC	1 cut(s)	514
PciI	ACATGT	3 cut(s)	1250, 2287, 4560
PcsI	WCGNNNNNNNCGW	3 cut(s)	201, 243, 2275
PctI	GAATGC	3 cut(s)	487, 3377, 3677
PdmI	GAANNNNTTC	5 cut(s)	877, 1621, 1651, 2520, 3074
PflMI	CCANNNNNTGG	1 cut(s)	4617
Ple19I	CGATCG	1 cut(s)	3698
PleI	GAGTC	3 cut(s)	1573, 2185, 3952
PluTI	GGCGCC	1 cut(s)	37
PpsI	GAGTC	3 cut(s)	1573, 2185, 3952
Ppu21I	YACGTR	2 cut(s)	1334, 2630
PpuMI	RGGWCCY	2 cut(s)	25, 533
PscI	ACATGT	3 cut(s)	1250, 2287, 4560
PshAI	GACNNNNGTC	3 cut(s)	552, 2012, 4409
PshBI	ATTAAT	1 cut(s)	3339
PsiI	TTATAA	2 cut(s)	2072, 2504
Psp5II	RGGWCCY	2 cut(s)	25, 533
Psp6I	CCWGG	7 cut(s)	988, 2167, 2469, 3023, 3262, 3829, 4204
PspEI	GGTNACC	1 cut(s)	984
PspFI	CCCAGC	1 cut(s)	3646
PspGI	CCWGG	7 cut(s)	988, 2167, 2469, 3023, 3262, 3829, 4204
PspPI	GGNCC	8 cut(s)	25, 64, 133, 161, 291, 533, 617, 1486
PspPPI	RGGWCCY	2 cut(s)	25, 533
PspXI	VCTCGAGB	1 cut(s)	706
PstI	CTGCAG	6 cut(s)	908, 2724, 2911, 3916, 4027, 4461
PstNI	CAGNNNCTG	3 cut(s)	153, 3350, 4022
PsuI	RGATCY	7 cut(s)	949, 1283, 1820, 3878, 4153, 4165, 4606
PvuI	CGATCG	1 cut(s)	3698
PvuII	CAGCTG	3 cut(s)	150, 2311, 3650
RseI	CAYNNNNRTG	4 cut(s)	1573, 1791, 2021, 3408
SacII	CCGCGG	1 cut(s)	623
SalI	GTCGAC	1 cut(s)	1026
Sau96I	GGNCC	8 cut(s)	25, 64, 133, 161, 291, 533, 617, 1486
ScaI	AGTACT	2 cut(s)	3212, 3606
SchI	GAGTC	3 cut(s)	1574, 2186, 3953
ScrFI	CCNGG	7 cut(s)	990, 2169, 2471, 3025, 3264, 3831, 4206
SduI	GDGCHC	6 cut(s)	1090, 1240, 1342, 2378, 3775, 4582
SexAI	ACCWGGT	1 cut(s)	3262
SfaNI	GCATC	7 cut(s)	321, 797, 1434, 1828, 2041, 2760, 3356
SfcI	CTRYAG	8 cut(s)	904, 1899, 2720, 2907, 3121, 3912, 4023, 4457
SfoI	GGCGCC	1 cut(s)	35
Sfr274I	CTCGAG	1 cut(s)	706
Sfr303I	CCGCGG	1 cut(s)	623
SgrBI	CCGCGG	1 cut(s)	623
SinI	GGWCC	5 cut(s)	25, 291, 533, 617, 1486
SlaI	CTCGAG	1 cut(s)	706
SmiI	ATTTAAAT	1 cut(s)	837
SmiMI	CAYNNNNRTG	4 cut(s)	1573, 1791, 2021, 3408
SmlI	CTYRAG	6 cut(s)	706, 1460, 2608, 2763, 4062, 4096
SmoI	CTYRAG	6 cut(s)	706, 1460, 2608, 2763, 4062, 4096
SnaBI	TACGTA	2 cut(s)	1334, 2630
SphI	GCATGC	1 cut(s)	1293
SspDI	GGCGCC	1 cut(s)	33
SspI	AATATT	4 cut(s)	492, 2042, 2208, 2519
SspMI	CTAG	9 cut(s)	1613, 1725, 2061, 2138, 2199, 2591, 2946, 4032, 4575
StyD4I	CCNGG	7 cut(s)	988, 2167, 2469, 3023, 3262, 3829, 4204
StyI	CCWWGG	3 cut(s)	3253, 3930, 4371
SwaI	ATTTAAAT	1 cut(s)	837
TaiI	ACGT	3 cut(s)	1336, 1724, 2632
TatI	WGTACW	7 cut(s)	1449, 1456, 1478, 1843, 3210, 3604, 3903
TauI	GCSGC	5 cut(s)	15, 138, 3746, 3749, 3764
TspGWI	ACGGA	1 cut(s)	2251
Van91I	CCANNNNNTGG	1 cut(s)	4617
VneI	GTGCAC	1 cut(s)	1236
VpaK11BI	GGWCC	5 cut(s)	25, 291, 533, 617, 1486
VspI	ATTAAT	1 cut(s)	3339
XagI	CCTNNNNNAGG	1 cut(s)	4209
XbaI	TCTAGA	2 cut(s)	2590, 2945
XceI	RCATGY	9 cut(s)	1157, 1222, 1254, 1293, 1790, 2291, 2705, 3490, 4564
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1211, 3260
XhoI	CTCGAG	1 cut(s)	706
XmiI	GTMKAC	4 cut(s)	1027, 1806, 2361, 3120
XmnI	GAANNNNTTC	5 cut(s)	877, 1621, 1651, 2520, 3074
XspI	CTAG	9 cut(s)	1613, 1725, 2061, 2138, 2199, 2591, 2946, 4032, 4575
ZrmI	AGTACT	2 cut(s)	3212, 3606
Zsp2I	ATGCAT	3 cut(s)	765, 1710, 3595

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.