Rroxscaffold_5G00365510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019500
Physical Location & Seq
Reverse (-)
47099741 .. 47101975
2235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365510.1

Sequence Viewer

Length: 489 bp
ATGTGCATGAACCAATTCGGAATCGTCGATTCTTCTACTTTGCATGACGAACTTGATGCTTTTGAGGAGCTTGGTCTGGCAGATGTAGGGTTTTACTTGAATGTGAAGTTTTGCCACGAGTGTACCTCACACATAATCCAGGCCACGCTGACTAGCGCCATCGATAACAAGAAACCCCATACCAAACAAGAGAACAAGGTTCAAAATTTTGGCAATTCACCTAATGCTTCCTTGGCACTTTGGGCGAACATCGGGTTGCAGGGCTCTTCTGTTCCTCTCCCTTCTTTGGGCCACGGTGGCGGCACAGGTCACGAGCTCGGGCAACTTGGTGACCGAGCCGCCGTCCGGGTCTTCTACCGTTCTCTTGCCCCAAGCCCGTGGAAGGAACAATTTCACAATTTTAGTGACTTGGGTCACCATTTGACGTGGTGCTCCGGCATAAAGGCCCCCGTGAACTTGGCCTTAGAACTTCAATATGTACGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.7

Weight (kDa)

6.1

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019500)

Species Orthologous Gene IDs
rosa_laevigata RLG00000034255
rosa_multiflora Rmu_sc0001290.1_g000012
rosa_roxburghii Rroxscaffold_5G00365510
rosa_samantha Rh2CG249500 Rh4DG246000 Rh5CG119600 Rh5DG106400 Rh5DG246900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 300, 339
AcsI RAATTY 1 cut(s) 205
AfaI GTAC 2 cut(s) 124, 480
AfiI CCNNNNNNNGG 4 cut(s) 286, 287, 345, 382
AgsI TTSAA 3 cut(s) 100, 203, 473
AjiI CACGTC 1 cut(s) 426
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 2 cut(s) 70, 316
AluI AGCT 2 cut(s) 70, 316
Alw21I GWGCWC 2 cut(s) 318, 434
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 4 cut(s) 141, 289, 444, 459
ApoI RAATTY 1 cut(s) 205
Asp700I GAANNNNTTC 2 cut(s) 14, 390
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 289, 445
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 3 cut(s) 210, 341, 407
AvaI CYCGRG 1 cut(s) 317
BaeI ACNNNNGTAYC 2 cut(s) 106, 139
BanII GRGCYC 2 cut(s) 266, 318
BauI CACGAG 2 cut(s) 116, 311
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 2 cut(s) 318, 434
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 326
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 1 cut(s) 347
BfaI CTAG 2 cut(s) 153, 487
BfoI RGCGCY 1 cut(s) 159
BglI GCCNNNNNGGC 1 cut(s) 297
BisI GCNGC 2 cut(s) 301, 339
BlsI GCNGC 2 cut(s) 302, 340
Bme1390I CCNGG 2 cut(s) 140, 347
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 426
BmgT120I GGNCC 2 cut(s) 289, 445
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 2 cut(s) 140, 347
BmsI GCATC 1 cut(s) 46
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 343
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpuMI CCSGG 1 cut(s) 347
Bsa29I ATCGAT 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 231, 292, 377
Bsc4I CCNNNNNNNGG 4 cut(s) 286, 287, 345, 382
BseBI CCWGG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 231, 292, 377
BseLI CCNNNNNNNGG 4 cut(s) 286, 287, 345, 382
BseRI GAGGAG 1 cut(s) 80
BshFI GGCC 4 cut(s) 143, 291, 446, 461
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 2 cut(s) 318, 434
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 346, 435
BslI CCNNNNNNNGG 4 cut(s) 286, 287, 345, 382
BsnI GGCC 4 cut(s) 143, 291, 446, 461
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 266, 318, 434
BspACI CCGC 2 cut(s) 300, 339
BspANI GGCC 4 cut(s) 143, 291, 446, 461
BspDI ATCGAT 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 447
BspQI GCTCTTC 1 cut(s) 271
BssECI CCNNGG 3 cut(s) 231, 292, 377
BssSI CACGAG 2 cut(s) 116, 311
BssT1I CCWWGG 1 cut(s) 231
Bst2BI CACGAG 2 cut(s) 116, 311
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 296, 359
Bst6I CTCTTC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 463
BstDSI CCRYGG 2 cut(s) 292, 377
BstEII GGTNACC 2 cut(s) 329, 413
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstMWI GCNNNNNNNGC 3 cut(s) 233, 242, 297
BstNI CCWGG 1 cut(s) 140
BstPAI GACNNNNGTC 1 cut(s) 411
BstPI GGTNACC 2 cut(s) 329, 413
BstSCI CCNGG 2 cut(s) 138, 345
BstV2I GAAGAC 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 378
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 4 cut(s) 143, 291, 446, 461
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 2 cut(s) 292, 377
BtrI CACGTC 1 cut(s) 426
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 289, 445
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 2 cut(s) 123, 479
CviAII CATG 2 cut(s) 7, 44
CviQI GTAC 2 cut(s) 123, 479
DdeI CTNAG 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 2 cut(s) 266, 318
Eco53kI GAGCTC 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 317
Eco91I GGTNACC 2 cut(s) 329, 413
EcoICRI GAGCTC 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 445
EcoO65I GGTNACC 2 cut(s) 329, 413
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 2 cut(s) 266, 318
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 2 cut(s) 10, 47
FaiI YATR 6 cut(s) 8, 45, 134, 180, 440, 477
FatI CATG 2 cut(s) 6, 43
Fnu4HI GCNGC 2 cut(s) 301, 339
FriOI GRGCYC 2 cut(s) 266, 318
Fsp4HI GCNGC 2 cut(s) 301, 339
FspBI CTAG 2 cut(s) 153, 487
GlaI GCGC 1 cut(s) 157
GluI GCNGC 2 cut(s) 301, 339
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 4 cut(s) 143, 291, 446, 461
HapII CCGG 2 cut(s) 346, 435
HhaI GCGC 1 cut(s) 158
Hin1II CATG 2 cut(s) 10, 47
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HinfI GANTC 2 cut(s) 21, 29
HpaII CCGG 2 cut(s) 346, 435
HphI GGTGA 3 cut(s) 210, 341, 407
Hpy166II GTNNAC 2 cut(s) 123, 454
Hpy188I TCNGA 1 cut(s) 20
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 123, 454
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 291, 376
HpyCH4III ACNGT 2 cut(s) 296, 359
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 3 cut(s) 6, 43, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 242, 297
HpyF3I CTNAG 1 cut(s) 463
HpySE526I ACGT 1 cut(s) 425
Hsp92II CATG 2 cut(s) 10, 47
HspAI GCGC 1 cut(s) 156
LguI GCTCTTC 1 cut(s) 271
LmnI GCTCC 2 cut(s) 67, 437
LpnPI CCDG 7 cut(s) 62, 125, 152, 245, 291, 359, 448
LweI GCATC 1 cut(s) 46
MaeI CTAG 2 cut(s) 153, 487
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 4 cut(s) 308, 329, 404, 413
MboII GAAGA 3 cut(s) 24, 258, 343
MhlI GDGCHC 3 cut(s) 266, 318, 434
MluCI AATT 5 cut(s) 14, 205, 214, 389, 397
MnlI CCTC 3 cut(s) 58, 136, 285
MroXI GAANNNNTTC 2 cut(s) 14, 390
MspI CCGG 2 cut(s) 346, 435
MspR9I CCNGG 2 cut(s) 140, 347
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 233, 242, 297
NciI CCSGG 1 cut(s) 347
NlaIII CATG 2 cut(s) 10, 47
NlaIV GGNNCC 1 cut(s) 447
NmuCI GTSAC 4 cut(s) 308, 329, 404, 413
PciSI GCTCTTC 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 14, 390
PfeI GAWTC 2 cut(s) 21, 29
PkrI GCNGC 2 cut(s) 302, 340
PshAI GACNNNNGTC 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 138
PspEI GGTNACC 2 cut(s) 329, 413
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 447
PspPI GGNCC 2 cut(s) 289, 445
RsaI GTAC 2 cut(s) 124, 480
RsaNI GTAC 2 cut(s) 123, 479
SacI GAGCTC 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 271
SatI GCNGC 2 cut(s) 301, 339
Sau96I GGNCC 2 cut(s) 289, 445
ScrFI CCNGG 2 cut(s) 140, 347
SduI GDGCHC 3 cut(s) 266, 318, 434
SetI ASST 7 cut(s) 72, 128, 201, 223, 310, 318, 428
SfaNI GCATC 1 cut(s) 46
Sse9I AATT 5 cut(s) 14, 205, 214, 389, 397
SsiI CCGC 2 cut(s) 300, 339
SspMI CTAG 2 cut(s) 153, 487
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 138, 345
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 2 cut(s) 296, 359
TaiI ACGT 1 cut(s) 428
TaqI TCGA 2 cut(s) 27, 162
TaqII GACCGA 1 cut(s) 348
TasI AATT 5 cut(s) 14, 205, 214, 389, 397
TauI GCSGC 2 cut(s) 303, 341
TfiI GAWTC 2 cut(s) 21, 29
TseFI GTSAC 4 cut(s) 308, 329, 404, 413
Tsp45I GTSAC 4 cut(s) 308, 329, 404, 413
TspDTI ATGAA 1 cut(s) 23
XapI RAATTY 1 cut(s) 205
XmnI GAANNNNTTC 2 cut(s) 14, 390
XspI CTAG 2 cut(s) 153, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.