Rroxscaffold_5G00366180

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016671
Physical Location & Seq
Forward (+)
48023505 .. 48024228
724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00366180.1

Sequence Viewer

Length: 564 bp
ATGATCTCCACTCCAACTGTGTCGTCGTTGCGAGCTTCGAAGCCGGAGACAACCTCAAAGGACCAGATCGAATGCACAATGTGCGCCGCATGCGAAAACCCATGCTACCAAGTCCCGTCGCCGCCGCCACCTTCGCCTCCTCCACCGTCAAAGCCTCCACCATCTTCCTCATCCTCACCCATCTGCCCACCTCCTCCGGCACAACCTAGCTCCGGCACGACCTACAACTCTCCCCCGCCGCCTTCCCAGCCCACTTACACTTACTACCCGCCGCCGGCCGGAGGCGCGTTTTATCCGCCGCCGAATGGCCAGAACTACCCGGGGCCGCCGCCGCCTAACCCAATTGTGCCCTACTTCCCGTTTTACTATCACACCCCTCCTCCCGGAGTGACAGAGAAGATCCAAAGTGATGGTGCTGATGAGTTCTTGATCTTGCAGAAGAGTGAAGAAGAGGAAACAGAGGACTTTGCATTTCCCAAAATGCCCTCGTGCAACAATGAACAAAAGTGCCTGTGCAGTGTTTACTGGAAATTTGAGCTTCTTTCCACTGCACTAGTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.23

Weight (kDa)

4.71

Isoelectric Point (pI)

100.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06750 AT5G49280
fragaria_vesca FvH4_4g19250
prunus_persica Prupe.1G126200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0424231
rosa_laevigata RLG00000007487
rosa_multiflora Rmu_sc0002260.1_g000029
rosa_roxburghii Rroxscaffold_5G00366180
rosa_rugosa Rorug04G0194500
rosa_samantha Rh4AG251900 Rh4BG257300 Rh4CG267800 Rh4DG251600
rosa_wichuraiana Rw4G021720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 287
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 2 cut(s) 276, 307
AcsI RAATTY 1 cut(s) 530
AdeI CACNNNGTG 1 cut(s) 81
AfiI CCNNNNNNNGG 6 cut(s) 212, 274, 278, 281, 305, 383
AhlI ACTAGT 1 cut(s) 553
AluBI AGCT 3 cut(s) 35, 210, 538
AluI AGCT 3 cut(s) 35, 210, 538
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 3 cut(s) 276, 307, 323
ApoI RAATTY 1 cut(s) 530
AspLEI GCGC 2 cut(s) 86, 287
AspS9I GGNCC 2 cut(s) 61, 323
AsuC2I CCSGG 3 cut(s) 320, 321, 384
AsuHPI GGTGA 1 cut(s) 168
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 351
BalI TGGCCA 1 cut(s) 309
BauI CACGAG 1 cut(s) 487
BccI CCATC 3 cut(s) 169, 188, 404
BcnI CCSGG 3 cut(s) 320, 321, 384
BcoDI GTCTC 1 cut(s) 41
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 2 cut(s) 207, 554
BisI GCNGC 9 cut(s) 87, 122, 125, 239, 272, 299, 326, 329, 332
BlsI GCNGC 9 cut(s) 88, 123, 126, 240, 273, 300, 327, 330, 333
Bme1390I CCNGG 3 cut(s) 320, 321, 384
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 61, 323
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 3 cut(s) 320, 321, 384
BplI GAGNNNNNCTC 2 cut(s) 38, 70
Bpu14I TTCGAA 1 cut(s) 38
BpuMI CCSGG 3 cut(s) 320, 321, 384
BsaJI CCNNGG 2 cut(s) 319, 320
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bsc4I CCNNNNNNNGG 6 cut(s) 212, 274, 278, 281, 305, 383
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 1 cut(s) 530
BseDI CCNNGG 2 cut(s) 319, 320
BseGI GGATG 1 cut(s) 170
BseLI CCNNNNNNNGG 6 cut(s) 212, 274, 278, 281, 305, 383
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 3 cut(s) 129, 183, 369
BseSI GKGCMC 1 cut(s) 351
BseX3I CGGCCG 1 cut(s) 276
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 2 cut(s) 534, 535
Bsh1236I CGCG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 3 cut(s) 278, 309, 325
BsiEI CGRYCG 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 7 cut(s) 44, 197, 213, 275, 279, 320, 384
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 6 cut(s) 212, 274, 278, 281, 305, 383
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 3 cut(s) 278, 309, 325
BsoBI CYCGRG 1 cut(s) 319
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 4 cut(s) 3, 66, 399, 429
BspANI GGCC 3 cut(s) 278, 309, 325
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 1 cut(s) 530
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 319, 320
BssMI GATC 4 cut(s) 3, 66, 399, 429
BssSI CACGAG 1 cut(s) 487
Bst2BI CACGAG 1 cut(s) 487
Bst4CI ACNGT 2 cut(s) 19, 147
Bst6I CTCTTC 2 cut(s) 434, 444
BstAPI GCANNNNNTGC 1 cut(s) 81
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 3 cut(s) 33, 91, 276
BstF5I GGATG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 2 cut(s) 86, 287
BstKTI GATC 4 cut(s) 6, 69, 402, 432
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 4 cut(s) 3, 66, 399, 429
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 6 cut(s) 81, 90, 133, 247, 284, 331
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 3 cut(s) 318, 319, 382
BstSLI GKGCMC 1 cut(s) 351
BstUI CGCG 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 1 cut(s) 399
BstZI CGGCCG 1 cut(s) 276
BsuRI GGCC 3 cut(s) 278, 309, 325
BtsCI GGATG 1 cut(s) 170
BtsI GCAGTG 2 cut(s) 523, 546
BtsIMutI CAGTG 2 cut(s) 523, 546
Cac8I GCNNGC 3 cut(s) 33, 91, 276
CfoI GCGC 2 cut(s) 86, 287
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 61, 323
Cfr9I CCCGGG 1 cut(s) 319
CviAII CATG 2 cut(s) 90, 102
CviJI RGCY 9 cut(s) 35, 43, 154, 210, 250, 278, 309, 325, 538
CviKI_1 RGCY 9 cut(s) 35, 43, 154, 210, 250, 278, 309, 325, 538
DpnI GATC 4 cut(s) 5, 68, 401, 431
DpnII GATC 4 cut(s) 3, 66, 399, 429
DraIII CACNNNGTG 1 cut(s) 81
EaeI YGGCCR 2 cut(s) 276, 307
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 2 cut(s) 434, 444
EarI CTCTTC 2 cut(s) 434, 444
EciI GGCGGA 1 cut(s) 285
EclXI CGGCCG 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 61
Eco52I CGGCCG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 319
FaeI CATG 2 cut(s) 93, 105
FaiI YATR 2 cut(s) 91, 103
FaqI GGGAC 1 cut(s) 98
FatI CATG 2 cut(s) 89, 101
FauI CCCGC 2 cut(s) 243, 276
Fnu4HI GCNGC 9 cut(s) 87, 122, 125, 239, 272, 299, 326, 329, 332
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 9 cut(s) 87, 122, 125, 239, 272, 299, 326, 329, 332
FspBI CTAG 2 cut(s) 207, 554
GlaI GCGC 2 cut(s) 85, 286
GluI GCNGC 9 cut(s) 87, 122, 125, 239, 272, 299, 326, 329, 332
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 3 cut(s) 278, 309, 325
HapII CCGG 7 cut(s) 44, 197, 213, 275, 279, 320, 384
HhaI GCGC 2 cut(s) 86, 287
Hin1II CATG 2 cut(s) 93, 105
Hin6I GCGC 2 cut(s) 84, 285
HinP1I GCGC 2 cut(s) 84, 285
HpaII CCGG 7 cut(s) 44, 197, 213, 275, 279, 320, 384
HphI GGTGA 1 cut(s) 168
Hpy166II GTNNAC 1 cut(s) 523
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 2 cut(s) 28, 121
HpyAV CCTTC 2 cut(s) 141, 252
HpyCH4III ACNGT 2 cut(s) 19, 147
HpyCH4V TGCA 6 cut(s) 75, 436, 470, 492, 516, 551
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 90, 133, 247, 284, 331
Hsp92II CATG 2 cut(s) 93, 105
HspAI GCGC 2 cut(s) 84, 285
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Kzo9I GATC 4 cut(s) 3, 66, 399, 429
LmnI GCTCC 1 cut(s) 215
MaeI CTAG 2 cut(s) 207, 554
MaeIII GTNAC 1 cut(s) 388
MalI GATC 4 cut(s) 5, 68, 401, 431
MboI GATC 4 cut(s) 3, 66, 399, 429
MboII GAAGA 5 cut(s) 156, 409, 451, 458, 461
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 399
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 2 cut(s) 342, 530
MluNI TGGCCA 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 38
Mox20I TGGCCA 1 cut(s) 309
MroNI GCCGGC 1 cut(s) 274
MscI TGGCCA 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 309
MspI CCGG 7 cut(s) 44, 197, 213, 275, 279, 320, 384
MspR9I CCNGG 3 cut(s) 320, 321, 384
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 77
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 6 cut(s) 81, 90, 133, 247, 284, 331
NaeI GCCGGC 1 cut(s) 276
NciI CCSGG 3 cut(s) 320, 321, 384
NdeII GATC 4 cut(s) 3, 66, 399, 429
NgoMIV GCCGGC 1 cut(s) 274
NlaIII CATG 2 cut(s) 93, 105
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 38
PaeI GCATGC 1 cut(s) 93
PctI GAATGC 1 cut(s) 77
PdiI GCCGGC 1 cut(s) 276
PfoI TCCNGGA 1 cut(s) 382
PkrI GCNGC 9 cut(s) 88, 123, 126, 240, 273, 300, 327, 330, 333
PspFI CCCAGC 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 2 cut(s) 61, 323
PsuI RGATCY 1 cut(s) 399
SatI GCNGC 9 cut(s) 87, 122, 125, 239, 272, 299, 326, 329, 332
Sau3AI GATC 4 cut(s) 3, 66, 399, 429
Sau96I GGNCC 2 cut(s) 61, 323
ScrFI CCNGG 3 cut(s) 320, 321, 384
SduI GDGCHC 1 cut(s) 351
SetI ASST 8 cut(s) 37, 56, 133, 193, 208, 212, 224, 540
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 61
SmaI CCCGGG 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 553
SphI GCATGC 1 cut(s) 93
Sse9I AATT 2 cut(s) 342, 530
SspMI CTAG 2 cut(s) 207, 554
StyD4I CCNGG 3 cut(s) 318, 319, 382
TaaI ACNGT 2 cut(s) 19, 147
TaqI TCGA 2 cut(s) 38, 69
TasI AATT 2 cut(s) 342, 530
TauI GCSGC 9 cut(s) 89, 124, 127, 241, 274, 301, 328, 331, 334
TscAI CASTG 2 cut(s) 523, 553
TseFI GTSAC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 513
TspMI CCCGGG 1 cut(s) 319
TspRI CASTG 2 cut(s) 523, 553
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 530
XceI RCATGY 1 cut(s) 93
XmaI CCCGGG 1 cut(s) 319
XspI CTAG 2 cut(s) 207, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.