Rroxscaffold_5G00374080

Sieve element occlusion C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010971
Physical Location & Seq
Reverse (-)
55156369 .. 55159894
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374080.1

Sequence Viewer

Length: 1002 bp
ATGGAAGTGGTTGGATTAGAAGAACCGCTCGGAAAGACCTTCGGTAAGATTTCACATGAGATACTTTGCAAGTGGCCTGATCAAGAAGATCCACACACAAGGACAATGCTATTATTTGATTTGCTTGGAAGTTATAAGTGGGATGCAAAAGTGGTATTAGTACTAGCATCTTTTGCAGCAAGTTACGGTGAATTGAGGCTCCTAATGCAGCTATGCTCTAGTAGCCCCTTGGCATTATCAGTCACAATGCTTAAGCAGTTGCCTGCTGACATGAGTCCTTTGAAGCCTCGATTTAAGGCCTTGAGTTTGTTAGTGAATGCAATGGTGGATGTAACCAAGTATATCATCAAGTTTGAAAACCTACCGCTTTCACATGTGGAGTTGGATCATGAGGCAAAGAATGCTACAAAGTCTATCATCTATATAGCTGTCTACTGGATCATTAGAGGCATCTTGAGATGCGCTTCGCAAATCACAGACTCAACACTTCTGAAATCTGAGCAATATTCAGATTCAACAATAATTGCAACATGGGAGGTCTTAAGTTTGGTGTATCGGCTGCGCAGCATTTACAGTTGCCTCAGACAGCAGGTGGAAATCTGTCATCAGCAAACAGAGACAAAGCTATATTGGAAGCTGATGAATGTCTTCCAGGAAACTCAAGTGGACAACCAAGAGGTGCTTCGATTCTTATTCGCTATGAGGGATGACTTGCCACTCAAGGATTGCTCCTCACAAGCAAAGCTTGGTGTATCTGATCTGAAGGGCAAGATAGTCATACTCTTGTTTTCAAGGCCAGAGTTCCTTTCGATAGACAAAACATTATTGTTGGTTCAGCAAACACATAATCATCCTCACTACAAAAATCTAGAGGGAAGTTACAGGATCGTATGGATCCCCATTCCAGTTTCCAGTCAATGGACCACTGCTGATGCGACAATGTTTGATTTCTTATCAAGTATTTTTCCCTTGGTACTCAGTAAGGAAACCCTGGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

333

Amino Acids

38.04

Weight (kDa)

6.71

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 6 - 220 8e-66 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 135
AarI CACCTGC 1 cut(s) 580
Acc16I TGCGCA 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 918
AccBSI CCGCTC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 432
AciI CCGC 2 cut(s) 26, 365
AclWI GGATC 6 cut(s) 83, 393, 446, 889, 893, 902
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 2 cut(s) 162, 975
AfiI CCNNNNNNNGG 1 cut(s) 918
AflII CTTAAG 2 cut(s) 251, 541
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 4 cut(s) 283, 356, 516, 792
AjnI CCWGG 2 cut(s) 651, 990
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 5 cut(s) 211, 428, 625, 637, 745
AluI AGCT 5 cut(s) 211, 428, 625, 637, 745
Alw26I GTCTC 1 cut(s) 611
AlwI GGATC 6 cut(s) 83, 393, 446, 889, 893, 902
AoxI GGCC 3 cut(s) 74, 297, 794
ApeKI GCWGC 4 cut(s) 176, 208, 559, 564
Asp700I GAANNNNTTC 1 cut(s) 647
AspLEI GCGC 2 cut(s) 464, 564
AspS9I GGNCC 1 cut(s) 921
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 921
BamHI GGATCC 1 cut(s) 894
BbsI GAAGAC 1 cut(s) 640
BbvI GCAGC 4 cut(s) 188, 220, 546, 576
BciT130I CCWGG 2 cut(s) 653, 992
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 3 cut(s) 164, 219, 869
BfrI CTTAAG 2 cut(s) 251, 541
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 4 cut(s) 177, 209, 560, 565
BlsI GCNGC 4 cut(s) 178, 210, 561, 566
BmcAI AGTACT 1 cut(s) 162
Bme1390I CCNGG 2 cut(s) 653, 992
Bme18I GGWCC 1 cut(s) 921
BmgT120I GGNCC 1 cut(s) 921
BmiI GGNNCC 2 cut(s) 200, 896
BmrFI CCNGG 2 cut(s) 653, 992
BmsI GCATC 5 cut(s) 133, 176, 449, 459, 922
BoxI GACNNNNGTC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 640
BpuEI CTTGAG 4 cut(s) 322, 475, 645, 704
BsaJI CCNNGG 3 cut(s) 228, 969, 990
Bsc4I CCNNNNNNNGG 1 cut(s) 918
Bse1I ACTGG 3 cut(s) 440, 905, 912
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 2 cut(s) 653, 992
BseDI CCNNGG 3 cut(s) 228, 969, 990
BseGI GGATG 4 cut(s) 148, 334, 712, 850
BseLI CCNNNNNNNGG 1 cut(s) 918
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 3 cut(s) 489, 595, 991
BseNI ACTGG 3 cut(s) 440, 905, 912
BseRI GAGGAG 1 cut(s) 721
BseXI GCAGC 4 cut(s) 188, 220, 546, 576
BshFI GGCC 3 cut(s) 76, 299, 796
BslI CCNNNNNNNGG 1 cut(s) 918
BsmAI GTCTC 1 cut(s) 611
BsmI GAATGC 2 cut(s) 322, 406
BsnI GGCC 3 cut(s) 76, 299, 796
Bsp143I GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
BspACI CCGC 2 cut(s) 26, 365
BspANI GGCC 3 cut(s) 76, 299, 796
BspCNI CTCAG 3 cut(s) 490, 594, 990
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 200, 896
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 6 cut(s) 83, 393, 446, 889, 893, 902
BspTI CTTAAG 2 cut(s) 251, 541
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 327
BsrI ACTGG 3 cut(s) 440, 905, 912
BssECI CCNNGG 3 cut(s) 228, 969, 990
BssMI GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
BssT1I CCWWGG 2 cut(s) 228, 969
Bst2UI CCWGG 2 cut(s) 653, 992
Bst4CI ACNGT 2 cut(s) 188, 575
BstAFI CTTAAG 2 cut(s) 251, 541
BstAPI GCANNNNNTGC 2 cut(s) 173, 401
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 498, 581, 977
BstF5I GGATG 4 cut(s) 148, 334, 712, 850
BstHHI GCGC 2 cut(s) 464, 564
BstKTI GATC 7 cut(s) 82, 91, 388, 441, 760, 888, 897
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
BstMWI GCNNNNNNNGC 4 cut(s) 173, 205, 222, 401
BstNI CCWGG 2 cut(s) 653, 992
BstNSI RCATGY 1 cut(s) 377
BstPAI GACNNNNGTC 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 651, 990
BstV1I GCAGC 4 cut(s) 188, 220, 546, 576
BstV2I GAAGAC 1 cut(s) 640
BstX2I RGATCY 2 cut(s) 88, 894
BstYI RGATCY 2 cut(s) 88, 894
BsuRI GGCC 3 cut(s) 76, 299, 796
BtsCI GGATG 4 cut(s) 148, 334, 712, 850
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 1 cut(s) 924
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 1 cut(s) 264
CciI TCATGA 1 cut(s) 388
CfoI GCGC 2 cut(s) 464, 564
Cfr13I GGNCC 1 cut(s) 921
Csp6I GTAC 2 cut(s) 161, 974
CviAII CATG 5 cut(s) 56, 271, 374, 389, 531
CviQI GTAC 2 cut(s) 161, 974
DdeI CTNAG 3 cut(s) 498, 581, 977
DpnI GATC 7 cut(s) 81, 90, 387, 440, 759, 887, 896
DpnII GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
Eco130I CCWWGG 2 cut(s) 228, 969
Eco147I AGGCCT 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 921
Eco57I CTGAAG 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 651, 990
EcoT14I CCWWGG 2 cut(s) 228, 969
ErhI CCWWGG 2 cut(s) 228, 969
FaeI CATG 5 cut(s) 59, 274, 377, 392, 534
FalI AAGNNNNNCTT 4 cut(s) 666, 698, 729, 761
FatI CATG 5 cut(s) 55, 270, 373, 388, 530
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 4 cut(s) 177, 209, 560, 565
FokI GGATG 4 cut(s) 155, 341, 719, 837
Fsp4HI GCNGC 4 cut(s) 177, 209, 560, 565
FspBI CTAG 3 cut(s) 164, 219, 869
FspI TGCGCA 1 cut(s) 563
GlaI GCGC 2 cut(s) 463, 563
GluI GCNGC 4 cut(s) 177, 209, 560, 565
HaeIII GGCC 3 cut(s) 76, 299, 796
HhaI GCGC 2 cut(s) 464, 564
Hin1II CATG 5 cut(s) 59, 274, 377, 392, 534
Hin6I GCGC 2 cut(s) 462, 562
HinP1I GCGC 2 cut(s) 462, 562
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 4 cut(s) 274, 479, 512, 687
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 433, 667
Hpy188I TCNGA 7 cut(s) 32, 492, 499, 511, 584, 757, 762
Hpy188III TCNNGA 4 cut(s) 83, 389, 454, 869
Hpy8I GTNNAC 2 cut(s) 433, 667
HpyAV CCTTC 2 cut(s) 49, 757
HpyCH4III ACNGT 2 cut(s) 188, 575
HpyCH4V TGCA 6 cut(s) 69, 146, 176, 208, 320, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 205, 222, 401
HpyF3I CTNAG 3 cut(s) 498, 581, 977
Hsp92II CATG 5 cut(s) 59, 274, 377, 392, 534
HspAI GCGC 2 cut(s) 462, 562
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
LmnI GCTCC 2 cut(s) 204, 734
Lsp1109I GCAGC 4 cut(s) 188, 220, 546, 576
LweI GCATC 5 cut(s) 133, 176, 449, 459, 922
MaeI CTAG 3 cut(s) 164, 219, 869
MaeIII GTNAC 4 cut(s) 182, 241, 331, 878
MalI GATC 7 cut(s) 81, 90, 387, 440, 759, 887, 896
MbiI CCGCTC 1 cut(s) 28
MboI GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
MboII GAAGA 3 cut(s) 32, 98, 640
MflI RGATCY 2 cut(s) 88, 894
MluCI AATT 2 cut(s) 191, 522
MlyI GAGTC 2 cut(s) 283, 473
MmeI TCCRAC 1 cut(s) 363
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 4 cut(s) 252, 294, 542, 1000
MspCI CTTAAG 2 cut(s) 251, 541
MspR9I CCNGG 2 cut(s) 653, 992
Mva1269I GAATGC 2 cut(s) 322, 406
MvaI CCWGG 2 cut(s) 653, 992
MwoI GCNNNNNNNGC 4 cut(s) 173, 205, 222, 401
NdeII GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
NlaIII CATG 5 cut(s) 59, 274, 377, 392, 534
NlaIV GGNNCC 2 cut(s) 200, 896
NmuCI GTSAC 1 cut(s) 241
NsbI TGCGCA 1 cut(s) 563
NspI RCATGY 1 cut(s) 377
PagI TCATGA 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 580
PceI AGGCCT 1 cut(s) 299
PciI ACATGT 1 cut(s) 373
PctI GAATGC 2 cut(s) 322, 406
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 2 cut(s) 512, 687
PflMI CCANNNNNTGG 1 cut(s) 918
PfoI TCCNGGA 1 cut(s) 651
PkrI GCNGC 4 cut(s) 178, 210, 561, 566
PleI GAGTC 2 cut(s) 282, 473
PpsI GAGTC 2 cut(s) 282, 473
PscI ACATGT 1 cut(s) 373
PshAI GACNNNNGTC 1 cut(s) 273
PsiI TTATAA 1 cut(s) 135
Psp6I CCWGG 2 cut(s) 651, 990
PspGI CCWGG 2 cut(s) 651, 990
PspN4I GGNNCC 2 cut(s) 200, 896
PspPI GGNCC 1 cut(s) 921
PsuI RGATCY 2 cut(s) 88, 894
RsaI GTAC 2 cut(s) 162, 975
RsaNI GTAC 2 cut(s) 161, 974
SaqAI TTAA 4 cut(s) 252, 294, 542, 1000
SatI GCNGC 4 cut(s) 177, 209, 560, 565
Sau3AI GATC 7 cut(s) 79, 88, 385, 438, 757, 885, 894
Sau96I GGNCC 1 cut(s) 921
ScaI AGTACT 1 cut(s) 162
SchI GAGTC 2 cut(s) 283, 473
ScrFI CCNGG 2 cut(s) 653, 992
SfaNI GCATC 5 cut(s) 133, 176, 449, 459, 922
SinI GGWCC 1 cut(s) 921
SmlI CTYRAG 6 cut(s) 251, 301, 454, 541, 660, 719
SmoI CTYRAG 6 cut(s) 251, 301, 454, 541, 660, 719
Sse9I AATT 2 cut(s) 191, 522
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 2 cut(s) 26, 365
SspI AATATT 1 cut(s) 506
SspMI CTAG 3 cut(s) 164, 219, 869
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 651, 990
StyI CCWWGG 2 cut(s) 228, 969
TaaI ACNGT 2 cut(s) 188, 575
TaqI TCGA 3 cut(s) 289, 685, 809
TasI AATT 2 cut(s) 191, 522
TatI WGTACW 1 cut(s) 160
TfiI GAWTC 2 cut(s) 512, 687
Tru1I TTAA 4 cut(s) 252, 294, 542, 1000
Tru9I TTAA 4 cut(s) 252, 294, 542, 1000
TscAI CASTG 1 cut(s) 931
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 4 cut(s) 176, 208, 559, 564
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 656
TspRI CASTG 1 cut(s) 931
Van91I CCANNNNNTGG 1 cut(s) 918
Vha464I CTTAAG 2 cut(s) 251, 541
VpaK11BI GGWCC 1 cut(s) 921
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 3 cut(s) 164, 219, 869
ZrmI AGTACT 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.