Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0011851

Physical Location & Seq

Genomic Coordinates Reverse (-)

11943650 .. 11947967

Gene Structure 4318 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00171640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2001 bp

ATGAACCTGGAGACCTCTCTCGCCGGCGGCAGCAACAACGTCACTTACGATGGCCGGTCTCTGATAATCGACGGTCAGCATAAGATTCTCTTCTCCGGTTCCATTCACTACCCCAGAAGCACTCCTCAGATGTGGCCATCTCTGATAGCCAAAGCCAAAGAAGGGGGAATAGATGTGATACAAACATACGTGTTTTGGAACCTTCATGAACCCCAAAAAGGACAGTTTGTTTTCAATGGAAGACGTGACATAGTAGCCTTTATAAAGGAAGTCCACAGACAAGGCCTTGGTTTTCCAGTTTGGTTAAGGAACATTCCGGGCATTGTTTTCCGGTCTGATAATGAACCGTATAAGGCTGAAATGCAGAGGTTTACAGCAAAAATAGTAGACCTAATGAAATCAAATGGACTGTACGCTTCACAAGGCGGACCGATTATATTGTCACAGATTGAGAACGAGTACCAAAATGTGGAAGCGGCGTTTCATGAAAAGGGACCTCCTTATGTTCTTTGGGCTGCATCCATGGCGGAGGGCCTTCAAACTGGTGTGCCCTGGGTTATGTGCAAGCAAAATGATGCTCCCGGCGCTGTGATCAATGCATGTAATGGGTTGAAGTGTGGAGAAACCTTTGCTGGCCCAAATGCCCCTAATAAGCCAGCAATTTGGACAGAGGACTGGACAAGCCAATACCTTGTATATGGTGAAGAACCATACCTCAGAAGAGCTGAAGATATTGCATTTCATGTTGCACTCTTTATTGCAAAGAATAAAGGAAGCTATGTAAACTACTACATGTATCATGGAGGAACCAATTTTGGAAGAACAACTTCCGAGTTTGTGACTACTAGTTATTACGATGAAGCTCCTCTTGATGAGTATGGTATACCAAAGGAACCAAAATGGACTCATCTCAAGGAGTTGCATGCTGCAGTGAAGTTGTCCATCAGTCCTCTACTTGCTGGAGAACAAACCGTTGTCAATTTGGGTCAATTTCAAACAGCCTATGTCTACAAAGCTCAATCTGGTGAATGTGCTGCTTTCTTGGTGAACAATGGCTCTAAAGATGCCACTGTCCAATTTCAGAACTCTCCTTATCAATTGTCTCCAAAGTCAATTAGTATCTTACCAGACTGTAAAAATGTGGCTTTCAACACTGGAAAGGTAAGCGCACAACATGCAACAAGATCATGGACACCAGCTCAAAAGTTTGATTCACCTCAGAATTGGCAAGAGTACAAAGAAGCCATTCCCACTTATGATGACACCTCTCTAAGAGAGAACATAATAGTAGACCAAATGAGAACTACAAATGATCGTTCTGATTATCTGTGGTACACTTTCAGCTTTCAGCATGACTCTCCTAATCCAGAATCAACATTAAAGGTGCAAACACGCGGACATATCCTGCATTCATTCGTAAACGGAGTACTTGTAGGATCTGGACATGGAGTGCTCAGGAATACAGGTTTTTCCTTGGAGAATAAGATTACTCTGAAAAAGGGGATAAACCATGTCTCCCTGCTAAATGCAATGGTTGGACTGCCGGACTCTGGAGCATATCTAGAGCGTAGAGATTACGGATTACAGAGTGTGAAGATTGACGATAAAGATTTCGGCACATATGCATGGGGATACCAGGTCGGGTTGTATGGAGAGAACTTACAAATCCATACAGACACAAGTAAAGTTCAATGGAACAGATTAAGCTATTCAAAACAACCACTGACATGGTATAAGACTTTATTTGATGCACCAGCAGGAAATGACCCTGTTGCATTATATCTTGGTTCCATGGGAAAGGGTGAAGTTTGGATCAATGGTCAAAGCATTGGTCGATATTGGGTCTCCTTCCACACCCCAAAAGGGACTCCTTCACAGACATGGTACCATGTACCTCGATCTTTCCTTAAGCCTACTGGAAACTTGTTAGTCCTCATTGAAGAAGAAACTGGAGACCCGGTTAAGATATCTCTGGACAAAGTAAATTATAGTTTACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

666

Amino Acids

74.69

Weight (kDa)

6.66

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	20 - 96	1.7e-30	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	97 - 310	6.5e-61	Glycosyl hydrolases family 35
GHD	PF17834	333 - 388	4.3e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	575 - 641	1.1e-24	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77410 AT1G77410 AT1G77410 AT1G77410
fragaria_vesca	FvH4_2g27470
prunus_persica	Prupe.1G492800_v2.0.a1 Prupe.1G492800_v2.0.a1 Prupe.1G492800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0296311
rosa_laevigata	RLG00000011707
rosa_multiflora	Rmu_sc0001478.1_g000001 Rmu_sc0004708.1_g000001 Rmu_sc0012554.1_g000001
rosa_roxburghii	Rroxscaffold_7G00171640 Rroxscaffold_7G00178440
rosa_rugosa	Rorug06G0261000
rosa_samantha	Rh6BG380800 Rh6CG386000
rosa_wichuraiana	Rw6G032490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	263
Acc65I	GGTACC	1 cut(s)	1884
AccB1I	GGYRCC	1 cut(s)	1884
AccB7I	CCANNNNNTGG	1 cut(s)	469
AccI	GTMKAC	4 cut(s)	387, 883, 1008, 1290
AccII	CGCG	1 cut(s)	1395
AciI	CCGC	5 cut(s)	27, 426, 476, 527, 1395
AclWI	GGATC	2 cut(s)	1444, 1820
AcoI	YGGCCR	2 cut(s)	52, 134
AcuI	CTGAAG	1 cut(s)	747
AfaI	GTAC	7 cut(s)	413, 461, 1235, 1334, 1428, 1886, 1893
AfiI	CCNNNNNNNGG	5 cut(s)	162, 218, 469, 1550, 1863
AflII	CTTAAG	1 cut(s)	1907
AflIII	ACRYGT	2 cut(s)	189, 792
AgsI	TTSAA	8 cut(s)	235, 539, 613, 995, 1150, 1691, 1713, 1940
AhlI	ACTAGT	1 cut(s)	845
AjiI	CACGTC	1 cut(s)	245
AjnI	CCWGG	3 cut(s)	6, 551, 1635
AluBI	AGCT	7 cut(s)	725, 777, 863, 1016, 1199, 1344, 1707
AluI	AGCT	7 cut(s)	725, 777, 863, 1016, 1199, 1344, 1707
Alw21I	GWGCWC	1 cut(s)	1455
Alw26I	GTCTC	6 cut(s)	5, 63, 1107, 1519, 1849, 1947
AlwI	GGATC	2 cut(s)	1444, 1820
AoxI	GGCC	5 cut(s)	52, 134, 283, 532, 634
ApeKI	GCWGC	4 cut(s)	30, 515, 926, 1034
ArsI	GACNNNNNNTTYG	2 cut(s)	1816, 1848
Asp700I	GAANNNNTTC	2 cut(s)	826, 1245
Asp718I	GGTACC	1 cut(s)	1884
AspLEI	GCGC	2 cut(s)	587, 1169
AspS9I	GGNCC	4 cut(s)	428, 494, 532, 635
AsuC2I	CCSGG	3 cut(s)	318, 582, 1958
AsuHPI	GGTGA	5 cut(s)	713, 1037, 1057, 1206, 1814
AvaII	GGWCC	2 cut(s)	428, 494
BaeGI	GKGCMC	1 cut(s)	552
BalI	TGGCCA	1 cut(s)	136
BanI	GGYRCC	1 cut(s)	1884
BbsI	GAAGAC	1 cut(s)	247
Bbv12I	GWGCWC	1 cut(s)	1455
BbvI	GCAGC	4 cut(s)	42, 502, 913, 1021
BccI	CCATC	3 cut(s)	44, 145, 950
BciT130I	CCWGG	3 cut(s)	8, 553, 1637
BciVI	GTATCC	1 cut(s)	1625
BclI	TGATCA	1 cut(s)	591
BcnI	CCSGG	3 cut(s)	318, 582, 1958
BcoDI	GTCTC	6 cut(s)	5, 63, 1107, 1519, 1849, 1947
BcuI	ACTAGT	1 cut(s)	845
BfaI	CTAG	2 cut(s)	846, 1562
BfmI	CTRYAG	1 cut(s)	927
BfoI	RGCGCY	1 cut(s)	588
BfrI	CTTAAG	1 cut(s)	1907
BfuI	GTATCC	1 cut(s)	1625
BisI	GCNGC	6 cut(s)	28, 31, 477, 516, 927, 1035
BlsI	GCNGC	6 cut(s)	29, 32, 478, 517, 928, 1036
BmcAI	AGTACT	1 cut(s)	1428
Bme1390I	CCNGG	6 cut(s)	8, 318, 553, 582, 1637, 1958
Bme18I	GGWCC	2 cut(s)	428, 494
BmgBI	CACGTC	1 cut(s)	245
BmgT120I	GGNCC	4 cut(s)	428, 494, 532, 635
BmiI	GGNNCC	7 cut(s)	100, 200, 495, 808, 894, 1789, 1886
BmrFI	CCNGG	6 cut(s)	8, 318, 553, 582, 1637, 1958
BmsI	GCATC	4 cut(s)	527, 565, 1054, 1738
BpiI	GAAGAC	1 cut(s)	247
BpmI	CTGGAG	4 cut(s)	29, 981, 1572, 1971
Bpu10I	CCTNAGC	1 cut(s)	1454
BpuEI	CTTGAG	1 cut(s)	896
BpuMI	CCSGG	3 cut(s)	318, 582, 1958
BsaAI	YACGTR	1 cut(s)	190
BsaI	GGTCTC	4 cut(s)	5, 63, 1849, 1947
BsaJI	CCNNGG	6 cut(s)	286, 522, 551, 552, 1473, 1791
BsaWI	WCCGGW	2 cut(s)	95, 330
BsaXI	ACNNNNNCTCC	6 cut(s)	1416, 1446, 1499, 1529, 1944, 1974
Bsc4I	CCNNNNNNNGG	5 cut(s)	162, 218, 469, 1550, 1863
Bse118I	RCCGGY	2 cut(s)	23, 54
Bse1I	ACTGG	6 cut(s)	296, 547, 680, 1159, 1921, 1954
Bse3DI	GCAATG	1 cut(s)	1536
BseBI	CCWGG	3 cut(s)	8, 553, 1637
BseDI	CCNNGG	6 cut(s)	286, 522, 551, 552, 1473, 1791
BseGI	GGATG	1 cut(s)	518
BseLI	CCNNNNNNNGG	5 cut(s)	162, 218, 469, 1550, 1863
BseMI	GCAATG	1 cut(s)	1536
BseMII	CTCAG	4 cut(s)	140, 730, 1232, 1468
BseNI	ACTGG	6 cut(s)	296, 547, 680, 1159, 1921, 1954
BseRI	GAGGAG	2 cut(s)	114, 855
BseSI	GKGCMC	1 cut(s)	552
BseXI	GCAGC	4 cut(s)	42, 502, 913, 1021
Bsh1236I	CGCG	1 cut(s)	1395
BshFI	GGCC	5 cut(s)	54, 136, 285, 534, 636
BshNI	GGYRCC	1 cut(s)	1884
BsiHKAI	GWGCWC	1 cut(s)	1455
BsiSI	CCGG	8 cut(s)	24, 55, 96, 317, 331, 582, 1544, 1958
BslFI	GGGAC	2 cut(s)	507, 1879
BslI	CCNNNNNNNGG	5 cut(s)	162, 218, 469, 1550, 1863
BsmAI	GTCTC	6 cut(s)	5, 63, 1107, 1519, 1849, 1947
BsmFI	GGGAC	2 cut(s)	507, 1879
BsmI	GAATGC	1 cut(s)	1408
BsnI	GGCC	5 cut(s)	54, 136, 285, 534, 636
Bso31I	GGTCTC	4 cut(s)	5, 63, 1849, 1947
Bsp1286I	GDGCHC	2 cut(s)	552, 1455
Bsp143I	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
Bsp19I	CCATGG	2 cut(s)	522, 1791
BspACI	CCGC	5 cut(s)	27, 426, 476, 527, 1395
BspANI	GGCC	5 cut(s)	54, 136, 285, 534, 636
BspCNI	CTCAG	4 cut(s)	139, 729, 1231, 1467
BspFNI	CGCG	1 cut(s)	1395
BspHI	TCATGA	2 cut(s)	205, 484
BspLI	GGNNCC	7 cut(s)	100, 200, 495, 808, 894, 1789, 1886
BspMAI	CTGCAG	1 cut(s)	931
BspPI	GGATC	2 cut(s)	1444, 1820
BspQI	GCTCTTC	1 cut(s)	715
BspT107I	GGYRCC	1 cut(s)	1884
BspTI	CTTAAG	1 cut(s)	1907
BspTNI	GGTCTC	4 cut(s)	5, 63, 1849, 1947
BsrDI	GCAATG	1 cut(s)	1536
BsrFI	RCCGGY	2 cut(s)	23, 54
BsrI	ACTGG	6 cut(s)	296, 547, 680, 1159, 1921, 1954
BssAI	RCCGGY	2 cut(s)	23, 54
BssECI	CCNNGG	6 cut(s)	286, 522, 551, 552, 1473, 1791
BssMI	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
BssNAI	GTATAC	1 cut(s)	884
BssT1I	CCWWGG	4 cut(s)	286, 522, 1473, 1791
Bst1107I	GTATAC	1 cut(s)	884
Bst2UI	CCWGG	3 cut(s)	8, 553, 1637
Bst4CI	ACNGT	7 cut(s)	74, 225, 348, 411, 973, 1072, 1133
Bst6I	CTCTTC	2 cut(s)	95, 715
BstAFI	CTTAAG	1 cut(s)	1907
BstAPI	GCANNNNNTGC	1 cut(s)	1175
BstBAI	YACGTR	1 cut(s)	190
BstC8I	GCNNGC	5 cut(s)	25, 566, 634, 657, 924
BstDEI	CTNAG	5 cut(s)	126, 716, 1218, 1271, 1454
BstDSI	CCRYGG	2 cut(s)	522, 1791
BstF5I	GGATG	1 cut(s)	518
BstFNI	CGCG	1 cut(s)	1395
BstH2I	RGCGCY	1 cut(s)	588
BstHHI	GCGC	2 cut(s)	587, 1169
BstKTI	GATC	6 cut(s)	594, 1187, 1315, 1439, 1815, 1901
BstMAI	GTCTC	6 cut(s)	5, 63, 1107, 1519, 1849, 1947
BstMBI	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
BstMWI	GCNNNNNNNGC	3 cut(s)	524, 584, 1175
BstNI	CCWGG	3 cut(s)	8, 553, 1637
BstNSI	RCATGY	4 cut(s)	603, 796, 926, 1178
BstSCI	CCNGG	6 cut(s)	6, 316, 551, 580, 1635, 1956
BstSFI	CTRYAG	1 cut(s)	927
BstSLI	GKGCMC	1 cut(s)	552
BstUI	CGCG	1 cut(s)	1395
BstV1I	GCAGC	4 cut(s)	42, 502, 913, 1021
BstV2I	GAAGAC	1 cut(s)	247
BstX2I	RGATCY	1 cut(s)	1436
BstXI	CCANNNNNNTGG	2 cut(s)	663, 1728
BstYI	RGATCY	1 cut(s)	1436
BstZ17I	GTATAC	1 cut(s)	884
BsuI	GTATCC	1 cut(s)	1625
BsuRI	GGCC	5 cut(s)	54, 136, 285, 534, 636
BtgI	CCRYGG	2 cut(s)	522, 1791
BtrI	CACGTC	1 cut(s)	245
BtsCI	GGATG	1 cut(s)	518
BtsI	GCAGTG	1 cut(s)	936
BtsIMutI	CAGTG	4 cut(s)	936, 1068, 1152, 1721
Cac8I	GCNNGC	5 cut(s)	25, 566, 634, 657, 924
CciI	TCATGA	2 cut(s)	205, 484
CfoI	GCGC	2 cut(s)	587, 1169
Cfr10I	RCCGGY	2 cut(s)	23, 54
Cfr13I	GGNCC	4 cut(s)	428, 494, 532, 635
CpoI	CGGWCCG	1 cut(s)	428
CsiI	ACCWGGT	1 cut(s)	1635
Csp6I	GTAC	7 cut(s)	412, 460, 1234, 1333, 1427, 1885, 1892
CspI	CGGWCCG	1 cut(s)	428
CviQI	GTAC	7 cut(s)	412, 460, 1234, 1333, 1427, 1885, 1892
DdeI	CTNAG	5 cut(s)	126, 716, 1218, 1271, 1454
DpnI	GATC	6 cut(s)	593, 1186, 1314, 1438, 1814, 1900
DpnII	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
EaeI	YGGCCR	2 cut(s)	52, 134
Eam1104I	CTCTTC	2 cut(s)	95, 715
EarI	CTCTTC	2 cut(s)	95, 715
EciI	GGCGGA	2 cut(s)	441, 542
Eco130I	CCWWGG	4 cut(s)	286, 522, 1473, 1791
Eco147I	AGGCCT	1 cut(s)	285
Eco31I	GGTCTC	4 cut(s)	5, 63, 1849, 1947
Eco32I	GATATC	1 cut(s)	1968
Eco47I	GGWCC	2 cut(s)	428, 494
Eco57I	CTGAAG	1 cut(s)	747
EcoO109I	RGGNCCY	2 cut(s)	494, 532
EcoRII	CCWGG	3 cut(s)	6, 551, 1635
EcoRV	GATATC	1 cut(s)	1968
EcoT14I	CCWWGG	4 cut(s)	286, 522, 1473, 1791
EcoT22I	ATGCAT	2 cut(s)	601, 1627
ErhI	CCWWGG	4 cut(s)	286, 522, 1473, 1791
FalI	AAGNNNNNCTT	6 cut(s)	74, 106, 811, 843, 852, 884
FaqI	GGGAC	2 cut(s)	507, 1879
FauNDI	CATATG	1 cut(s)	1621
FbaI	TGATCA	1 cut(s)	591
FblI	GTMKAC	4 cut(s)	387, 883, 1008, 1290
Fnu4HI	GCNGC	6 cut(s)	28, 31, 477, 516, 927, 1035
FokI	GGATG	1 cut(s)	505
Fsp4HI	GCNGC	6 cut(s)	28, 31, 477, 516, 927, 1035
FspBI	CTAG	2 cut(s)	846, 1562
GlaI	GCGC	2 cut(s)	586, 1168
GluI	GCNGC	6 cut(s)	28, 31, 477, 516, 927, 1035
GsuI	CTGGAG	4 cut(s)	29, 981, 1572, 1971
HaeII	RGCGCY	1 cut(s)	588
HaeIII	GGCC	5 cut(s)	54, 136, 285, 534, 636
HapII	CCGG	8 cut(s)	24, 55, 96, 317, 331, 582, 1544, 1958
HhaI	GCGC	2 cut(s)	587, 1169
Hin6I	GCGC	2 cut(s)	585, 1167
HinP1I	GCGC	2 cut(s)	585, 1167
HinfI	GANTC	7 cut(s)	85, 904, 1211, 1355, 1370, 1547, 1867
HpaII	CCGG	8 cut(s)	24, 55, 96, 317, 331, 582, 1544, 1958
HphI	GGTGA	5 cut(s)	713, 1037, 1057, 1206, 1814
Hpy188III	TCNNGA	9 cut(s)	206, 485, 869, 1367, 1440, 1456, 1551, 1562, 1973
Hpy99I	CGWCG	1 cut(s)	74
HpyAV	CCTTC	5 cut(s)	155, 212, 545, 1858, 1881
HpyCH4III	ACNGT	7 cut(s)	74, 225, 348, 411, 973, 1072, 1133
HpyCH4IV	ACGT	3 cut(s)	39, 189, 244
HpyF10VI	GCNNNNNNNGC	3 cut(s)	524, 584, 1175
HpyF3I	CTNAG	5 cut(s)	126, 716, 1218, 1271, 1454
HpySE526I	ACGT	3 cut(s)	39, 189, 244
HspAI	GCGC	2 cut(s)	585, 1167
KpnI	GGTACC	1 cut(s)	1888
KroI	GCCGGC	1 cut(s)	23
KroNI	GCCGGC	1 cut(s)	25
Ksp22I	TGATCA	1 cut(s)	591
Kzo9I	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
LguI	GCTCTTC	1 cut(s)	715
LmnI	GCTCC	3 cut(s)	583, 868, 1553
Lsp1109I	GCAGC	4 cut(s)	42, 502, 913, 1021
LweI	GCATC	4 cut(s)	527, 565, 1054, 1738
MabI	ACCWGGT	1 cut(s)	1635
MaeI	CTAG	2 cut(s)	846, 1562
MaeII	ACGT	3 cut(s)	39, 189, 244
MaeIII	GTNAC	4 cut(s)	40, 245, 441, 838
MalI	GATC	6 cut(s)	593, 1186, 1314, 1438, 1814, 1900
MboI	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
MboII	GAAGA	9 cut(s)	82, 252, 716, 732, 740, 831, 1606, 1952, 1955
MfeI	CAATTG	1 cut(s)	1097
MflI	RGATCY	1 cut(s)	1436
MhlI	GDGCHC	2 cut(s)	552, 1455
MlsI	TGGCCA	1 cut(s)	136
MluCI	AATT	9 cut(s)	660, 811, 979, 989, 1076, 1097, 1113, 1222, 1984
MluNI	TGGCCA	1 cut(s)	136
MlyI	GAGTC	4 cut(s)	898, 1349, 1541, 1861
MmeI	TCCRAC	1 cut(s)	1516
Mox20I	TGGCCA	1 cut(s)	136
Mph1103I	ATGCAT	2 cut(s)	601, 1627
MreI	CGCCGGCG	1 cut(s)	23
MroNI	GCCGGC	1 cut(s)	23
MroXI	GAANNNNTTC	2 cut(s)	826, 1245
MscI	TGGCCA	1 cut(s)	136
MseI	TTAA	5 cut(s)	305, 1379, 1703, 1908, 1962
MslI	CAYNNNNRTG	3 cut(s)	1624, 1726, 1879
Msp20I	TGGCCA	1 cut(s)	136
MspCI	CTTAAG	1 cut(s)	1907
MspI	CCGG	8 cut(s)	24, 55, 96, 317, 331, 582, 1544, 1958
MspR9I	CCNGG	6 cut(s)	8, 318, 553, 582, 1637, 1958
MunI	CAATTG	1 cut(s)	1097
Mva1269I	GAATGC	1 cut(s)	1408
MvaI	CCWGG	3 cut(s)	8, 553, 1637
MvnI	CGCG	1 cut(s)	1395
MwoI	GCNNNNNNNGC	3 cut(s)	524, 584, 1175
NaeI	GCCGGC	1 cut(s)	25
NciI	CCSGG	3 cut(s)	318, 582, 1958
NcoI	CCATGG	2 cut(s)	522, 1791
NdeI	CATATG	1 cut(s)	1621
NdeII	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
NgoMIV	GCCGGC	1 cut(s)	23
NlaIV	GGNNCC	7 cut(s)	100, 200, 495, 808, 894, 1789, 1886
NmuCI	GTSAC	4 cut(s)	40, 245, 441, 838
NsiI	ATGCAT	2 cut(s)	601, 1627
NspI	RCATGY	4 cut(s)	603, 796, 926, 1178
PaeI	GCATGC	1 cut(s)	926
PagI	TCATGA	2 cut(s)	205, 484
PasI	CCCWGGG	1 cut(s)	552
PceI	AGGCCT	1 cut(s)	285
PciI	ACATGT	1 cut(s)	792
PciSI	GCTCTTC	1 cut(s)	715
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	1408
PdiI	GCCGGC	1 cut(s)	25
PdmI	GAANNNNTTC	2 cut(s)	826, 1245
PfeI	GAWTC	3 cut(s)	85, 1211, 1370
PflMI	CCANNNNNTGG	1 cut(s)	469
PkrI	GCNGC	6 cut(s)	29, 32, 478, 517, 928, 1036
PleI	GAGTC	4 cut(s)	898, 1349, 1541, 1861
PpsI	GAGTC	4 cut(s)	898, 1349, 1541, 1861
Ppu21I	YACGTR	1 cut(s)	190
PpuMI	RGGWCCY	1 cut(s)	494
PscI	ACATGT	1 cut(s)	792
PsiI	TTATAA	1 cut(s)	263
Psp5II	RGGWCCY	1 cut(s)	494
Psp6I	CCWGG	3 cut(s)	6, 551, 1635
PspGI	CCWGG	3 cut(s)	6, 551, 1635
PspN4I	GGNNCC	7 cut(s)	100, 200, 495, 808, 894, 1789, 1886
PspPI	GGNCC	4 cut(s)	428, 494, 532, 635
PspPPI	RGGWCCY	1 cut(s)	494
PstI	CTGCAG	1 cut(s)	931
PsuI	RGATCY	1 cut(s)	1436
RsaI	GTAC	7 cut(s)	413, 461, 1235, 1334, 1428, 1886, 1893
RsaNI	GTAC	7 cut(s)	412, 460, 1234, 1333, 1427, 1885, 1892
RseI	CAYNNNNRTG	3 cut(s)	1624, 1726, 1879
Rsr2I	CGGWCCG	1 cut(s)	428
RsrII	CGGWCCG	1 cut(s)	428
SapI	GCTCTTC	1 cut(s)	715
SaqAI	TTAA	5 cut(s)	305, 1379, 1703, 1908, 1962
SatI	GCNGC	6 cut(s)	28, 31, 477, 516, 927, 1035
Sau3AI	GATC	6 cut(s)	591, 1184, 1312, 1436, 1812, 1898
Sau96I	GGNCC	4 cut(s)	428, 494, 532, 635
ScaI	AGTACT	1 cut(s)	1428
SchI	GAGTC	4 cut(s)	898, 1349, 1541, 1861
ScrFI	CCNGG	6 cut(s)	8, 318, 553, 582, 1637, 1958
SduI	GDGCHC	2 cut(s)	552, 1455
SexAI	ACCWGGT	1 cut(s)	1635
SfaNI	GCATC	4 cut(s)	527, 565, 1054, 1738
SfcI	CTRYAG	1 cut(s)	927
SgrAI	CRCCGGYG	1 cut(s)	23
SinI	GGWCC	2 cut(s)	428, 494
SmiMI	CAYNNNNRTG	3 cut(s)	1624, 1726, 1879
SmlI	CTYRAG	2 cut(s)	911, 1907
SmoI	CTYRAG	2 cut(s)	911, 1907
SpeI	ACTAGT	1 cut(s)	845
SphI	GCATGC	1 cut(s)	926
Sse9I	AATT	9 cut(s)	660, 811, 979, 989, 1076, 1097, 1113, 1222, 1984
SseBI	AGGCCT	1 cut(s)	285
SsiI	CCGC	5 cut(s)	27, 426, 476, 527, 1395
SspMI	CTAG	2 cut(s)	846, 1562
StuI	AGGCCT	1 cut(s)	285
StyD4I	CCNGG	6 cut(s)	6, 316, 551, 580, 1635, 1956
StyI	CCWWGG	4 cut(s)	286, 522, 1473, 1791
TaaI	ACNGT	7 cut(s)	74, 225, 348, 411, 973, 1072, 1133
TaiI	ACGT	3 cut(s)	42, 192, 247
TaqI	TCGA	3 cut(s)	69, 1834, 1897
TaqII	GACCGA	1 cut(s)	445
TasI	AATT	9 cut(s)	660, 811, 979, 989, 1076, 1097, 1113, 1222, 1984
TatI	WGTACW	2 cut(s)	1233, 1426
TauI	GCSGC	2 cut(s)	30, 479
TfiI	GAWTC	3 cut(s)	85, 1211, 1370
Tru1I	TTAA	5 cut(s)	305, 1379, 1703, 1908, 1962
Tru9I	TTAA	5 cut(s)	305, 1379, 1703, 1908, 1962
TscAI	CASTG	4 cut(s)	936, 1075, 1159, 1728
TseFI	GTSAC	4 cut(s)	40, 245, 441, 838
TseI	GCWGC	4 cut(s)	30, 515, 926, 1034
Tsp45I	GTSAC	4 cut(s)	40, 245, 441, 838
TspGWI	ACGGA	2 cut(s)	1437, 1593
TspRI	CASTG	4 cut(s)	936, 1075, 1159, 1728
Van91I	CCANNNNNTGG	1 cut(s)	469
Vha464I	CTTAAG	1 cut(s)	1907
VpaK11BI	GGWCC	2 cut(s)	428, 494
XbaI	TCTAGA	1 cut(s)	1561
XceI	RCATGY	4 cut(s)	603, 796, 926, 1178
XmiI	GTMKAC	4 cut(s)	387, 883, 1008, 1290
XmnI	GAANNNNTTC	2 cut(s)	826, 1245
XspI	CTAG	2 cut(s)	846, 1562
ZrmI	AGTACT	1 cut(s)	1428
Zsp2I	ATGCAT	2 cut(s)	601, 1627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.