Rroxscaffold_7G00175960

CSC1-like protein At4g02900

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010682
Physical Location & Seq
Reverse (-)
15430264 .. 15434869
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175960.1

Sequence Viewer

Length: 2415 bp
ATGGCGGATCTTCTAGAAATCTTGACGTCAGCAGGTATTAACCTGGCGACAGCATTGGGGTTCCTTGTGGCGTTTGCTTTGCTGCGGCTTCAGCCCATCAACGACAGAGTCTACTTCCCGAAATGGTACAGAAAAGGGATCAGAGGGAGCCCGACGAGCTCCGGGACTTTTGCCAAGAGGTTTGTCAACTTGGATGCCATGACCTACATCAAGTTTCTGAATTGGATGCCTGCAGCACTGAGGATGCCGGAGCCTGAGCTCATTGATCACGCCGGGCTAGACTCTGCTGCCTACATTCGGATTTATGTACTTGGCTTGAAGATATTCTTTCCCATAACTGTTCTTTCACTTGCGGTTTTGATTCCTCTCAATTACACGAACGACACGCTGGAAAAATCCAAGCAAGTAGAATACAGCGACGTTGATAAAATCTCGATATCCAATGTCCCTAATGCATCTCTTAAGTTCATTGGACATCTCATATTGTCTTATGTCTTCTCAATTTGGACATGCTTTGTTTTATACCACGAGTACAAGACAGTGGCTACTAAAAGGCTTCAATTTTTAGCATCTCAAAAACGCCGTCCAGATCAATTTTCGGTTCTTGTGAGGAATGTACCACCAGATCCTGATGAATCAGTCAGTGAGCATATCGAACACTTCTTTTGCGTAAACCATCCCAATGACTATCTTACACATCAAGTTGTATATAATACAAATAAACTTGCAAAGTTGGTTGCACAGAAGAAGAGTTTGAAAAATAGGTTTGTGTACTACCAAAATAAGTATGAGAGAAATCCAGAGAAACCAAGGCCTACTACAAAGACAGGTTTCTTGGGCCTGTTGGGAGACAAAGTGGACGCAATTGATTACTACACAACTGAGATTGAAAAGTTGAGCGAAGAAATGCATAATAATGTAGCTTTCACGCAGGAGAAAAAAGAAAGAGAAAGAGTTGCGAGTGATCCTGAAGCCATAATGCCAGCAGCATTTGTTTCATTCAAATCGCGATGGGGAGCAGCTGTATGTGCTCAAACTCAGCAATCAAGTAATGCTACCATCTGGTTGACAGAGTGGGCTCCTGAGCCACGTGACATCTATTGGAGAAATCTTTCAATTCCATACGTTGAACTCAATGTGCGGAAACTGCTCATGGCCGTTGCTTTCTTTGTTCTCATTTCCTTTTTTATGATTCCAATAGCATTCGTCCAAACTCTAGCCAACATTGACAATCTTAAGAAGGCCCTTCCTTTCTTGGAGCCAATAATAGAGAAGAAGCAAGTTATGTCTGTCATCCAAGGATTTCTTCCAGGAATTGCATTGAAGATATTCCTTGCTATACTGCCTATGATTCTAATGCTCATGTCAAAGATAGAGGGTTATACATCACTCTCATCTCTGGATAGGAGATCTGCTGCGAAGTATTATTTATTCATACTTATCAACATGTTTCTGGGAAATATTGTTGCAGGAACAGCATTTGAGCAGCTTGAGGAATTTACCAGCCAGGCCTCATCAGAATATTTAAAAACTATTGGGAGGACCATGCCAATGAAAGGCACATTCTTCATCACTTATATAATGGTTGATGGTTGGAGTGGAATTGCCGCAGAAATCATCAGATTGGTTCCGCTAGCTCTATACCACTTCAAAAACACATTTTTGGTTAAGACAGAACAAGATAGGGAGGAAGCAATGAATCCTGGTTCCTTGGCATTTGCTACAAATGAACCTAGAATACAGTTCTATATGTTGCTGGGGCTTGTATATTCTGTTGTCACTCCTTTACTTCTTCCATTTATTCTTATCTTCTTTGCTTTTGCCTATGTGGTTTTCCGTCACCAGATTATTAATGTCTATGATCAGAAATATGAGAGTGGTGCAGCCTTCTGGCCGCGTGTGCATATGCGTGTAACTATTGGCCTAATCGGTCTGCAAGGGTCGTTTCGAACCAGCATTCGTGACATTTCCTTTACAGGTCAGATTGAATTTTCCAGTACAAGTTCCTTTGGTTATACATTTACTTCCGGTTTAGTCATTGCACACACCTTTTGTGTAAACTCTGCTATGATGCGAGTGATATATCTTTGCATGCTTTCCACTTTCCAGTGTGGTGACATCTCTAGAACTTTGCAAAGTCAGTTCCTCTCCAGAGGTTTCTGGAATGCTAATAAAGATGCAATGGTAAAGGATACAGTTGAACGAGCTACTGAACCAACCTTGAATTTGTTGGTCTATCTTAAGGATGCTTATGTACATCCTGACTTCAAGGGAGGGGAGATGTACAAACCCGAAGCCATTGACGAAGAAGAGAGAAATCCTCTTGTTCCTACAAAGAGAACATCTCAAATGGCATCTCAAACGGGTAGTAAACACGAGTCTGAAGGCTCCGAAGGACCTTTTTTGAAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

804

Amino Acids

91.51

Weight (kDa)

8.79

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 7 - 177 2.5e-38 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 198 - 370 1.4e-45 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 381 - 644 6.9e-82 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010682)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02900 AT4G02900 AT4G02900 AT4G02900
fragaria_vesca FvH4_2g23940 FvH4_2g23940
malus_domestica MD11G1289500.v1.1
prunus_persica Prupe.8G244700_v2.0.a1 Prupe.8G244700_v2.0.a1
pyrus_communis pycom11g25690
rosa_chinensis RchiOBHm_Chr6g0291571
rosa_laevigata RLG00000012106
rosa_roxburghii Rroxscaffold_7G00175960
rosa_rugosa Rorug06G0220300 Rorug06G0220400 Rorug06G0220400
rosa_samantha Rh6AG332700 Rh6CG345900 Rh6DG333100
rosa_wichuraiana Rw6G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 29
Acc36I ACCTGC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 111
AccII CGCG 2 cut(s) 1009, 1897
AciI CCGC 7 cut(s) 5, 85, 353, 1141, 1608, 1631, 1895
AclWI GGATC 4 cut(s) 15, 146, 620, 959
AcoI YGGCCR 2 cut(s) 1155, 1892
AcsI RAATTY 3 cut(s) 1496, 1988, 2224
AcuI CTGAAG 3 cut(s) 74, 990, 2403
AcvI CACGTG 1 cut(s) 1091
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 8 cut(s) 128, 309, 533, 618, 773, 1999, 2256, 2285
AfiI CCNNNNNNNGG 2 cut(s) 297, 1556
AflII CTTAAG 3 cut(s) 461, 1235, 2240
AflIII ACRYGT 1 cut(s) 1446
AjnI CCWGG 4 cut(s) 42, 1309, 1506, 1702
AjuI GAANNNNNNNTTGG 2 cut(s) 1290, 1322
AluBI AGCT 7 cut(s) 159, 259, 923, 1022, 1489, 1637, 2207
AluI AGCT 7 cut(s) 159, 259, 923, 1022, 1489, 1637, 2207
Alw21I GWGCWC 3 cut(s) 161, 261, 1033
Alw26I GTCTC 1 cut(s) 843
AlwI GGATC 4 cut(s) 15, 146, 620, 959
AlwNI CAGNNNCTG 1 cut(s) 629
AoxI GGCC 7 cut(s) 812, 838, 1155, 1242, 1509, 1892, 1921
ApeKI GCWGC 8 cut(s) 82, 233, 287, 986, 1019, 1415, 1486, 1883
ApoI RAATTY 3 cut(s) 1496, 1988, 2224
ArsI GACNNNNNNTTYG 2 cut(s) 568, 600
AseI ATTAAT 1 cut(s) 1851
Asp700I GAANNNNTTC 3 cut(s) 323, 1111, 1328
AspS9I GGNCC 4 cut(s) 838, 1243, 1542, 2396
AsuC2I CCSGG 2 cut(s) 163, 274
AsuHPI GGTGA 2 cut(s) 1832, 2126
AsuII TTCGAA 1 cut(s) 1948
AsuNHI GCTAGC 1 cut(s) 1633
AvaII GGWCC 2 cut(s) 1542, 2396
BanII GRGCYC 4 cut(s) 152, 161, 261, 1081
BauI CACGAG 2 cut(s) 527, 2375
BbrPI CACGTG 1 cut(s) 1091
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 3 cut(s) 161, 261, 1033
BbvI GCAGC 8 cut(s) 69, 245, 274, 998, 1031, 1402, 1498, 1895
BccI CCATC 5 cut(s) 104, 684, 1005, 1067, 1583
BceAI ACGGC 2 cut(s) 567, 1142
BciT130I CCWGG 4 cut(s) 44, 1311, 1508, 1704
BciVI GTATCC 1 cut(s) 2185
BclI TGATCA 2 cut(s) 265, 1861
BcnI CCSGG 2 cut(s) 163, 274
BcoDI GTCTC 1 cut(s) 843
BfaI CTAG 6 cut(s) 14, 278, 1217, 1634, 1734, 2124
BfmI CTRYAG 1 cut(s) 231
BfrI CTTAAG 3 cut(s) 461, 1235, 2240
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 1 cut(s) 2185
BglII AGATCT 1 cut(s) 1409
Bme1390I CCNGG 6 cut(s) 44, 163, 274, 1311, 1508, 1704
Bme18I GGWCC 2 cut(s) 1542, 2396
BmgT120I GGNCC 4 cut(s) 838, 1243, 1542, 2396
BmiI GGNNCC 8 cut(s) 62, 149, 252, 1080, 1260, 1629, 1708, 2389
BmrFI CCNGG 6 cut(s) 44, 163, 274, 1311, 1508, 1704
BmsI GCATC 9 cut(s) 184, 216, 234, 464, 578, 2061, 2167, 2236, 2363
BmtI GCTAGC 1 cut(s) 1637
BpiI GAAGAC 1 cut(s) 487
BplI GAGNNNNNCTC 4 cut(s) 2305, 2337, 2329, 2361
BpmI CTGGAG 1 cut(s) 2134
Bpu10I CCTNAGC 2 cut(s) 255, 1083
Bpu14I TTCGAA 1 cut(s) 1948
BpuEI CTTGAG 1 cut(s) 1511
BpuMI CCSGG 2 cut(s) 163, 274
BsaAI YACGTR 1 cut(s) 1091
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 3 cut(s) 809, 1297, 1710
BsaWI WCCGGW 1 cut(s) 2027
BsaXI ACNNNNNCTCC 4 cut(s) 840, 870, 1008, 1038
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 1556
Bse1I ACTGG 2 cut(s) 1995, 2107
Bse3DI GCAATG 3 cut(s) 1701, 2037, 2187
BseBI CCWGG 4 cut(s) 44, 1311, 1508, 1704
BseDI CCNNGG 3 cut(s) 809, 1297, 1710
BseGI GGATG 7 cut(s) 199, 231, 249, 676, 1293, 2251, 2257
BseLI CCNNNNNNNGG 2 cut(s) 297, 1556
BseMI GCAATG 3 cut(s) 1701, 2037, 2187
BseMII CTCAG 5 cut(s) 230, 246, 873, 1052, 1074
BseNI ACTGG 2 cut(s) 1995, 2107
BseXI GCAGC 8 cut(s) 69, 245, 274, 998, 1031, 1402, 1498, 1895
BseYI CCCAGC 1 cut(s) 1756
BsgI GTGCAG 1 cut(s) 1902
Bsh1236I CGCG 2 cut(s) 1009, 1897
BshFI GGCC 7 cut(s) 814, 840, 1157, 1244, 1511, 1894, 1923
BsiHKAI GWGCWC 3 cut(s) 161, 261, 1033
BsiSI CCGG 4 cut(s) 162, 248, 273, 2028
BslFI GGGAC 2 cut(s) 178, 431
BslI CCNNNNNNNGG 2 cut(s) 297, 1556
BsmAI GTCTC 1 cut(s) 843
BsmFI GGGAC 2 cut(s) 178, 431
BsmI GAATGC 3 cut(s) 1202, 1956, 2170
BsnI GGCC 7 cut(s) 814, 840, 1157, 1244, 1511, 1894, 1923
Bsp119I TTCGAA 1 cut(s) 1948
Bsp1286I GDGCHC 5 cut(s) 152, 161, 261, 1033, 1081
Bsp1407I TGTACA 2 cut(s) 2254, 2283
Bsp143I GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
Bsp68I TCGCGA 1 cut(s) 1009
BspACI CCGC 7 cut(s) 5, 85, 353, 1141, 1608, 1631, 1895
BspANI GGCC 7 cut(s) 814, 840, 1157, 1244, 1511, 1894, 1923
BspCNI CTCAG 5 cut(s) 231, 247, 874, 1051, 1075
BspFNI CGCG 2 cut(s) 1009, 1897
BspLI GGNNCC 8 cut(s) 62, 149, 252, 1080, 1260, 1629, 1708, 2389
BspMAI CTGCAG 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 23
BspOI GCTAGC 1 cut(s) 1637
BspPI GGATC 4 cut(s) 15, 146, 620, 959
BspT104I TTCGAA 1 cut(s) 1948
BspTI CTTAAG 3 cut(s) 461, 1235, 2240
BsrDI GCAATG 3 cut(s) 1701, 2037, 2187
BsrGI TGTACA 2 cut(s) 2254, 2283
BsrI ACTGG 2 cut(s) 1995, 2107
BssECI CCNNGG 3 cut(s) 809, 1297, 1710
BssMI GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 2 cut(s) 527, 2375
BssT1I CCWWGG 3 cut(s) 809, 1297, 1710
Bst2BI CACGAG 2 cut(s) 527, 2375
Bst2UI CCWGG 4 cut(s) 44, 1311, 1508, 1704
Bst4CI ACNGT 4 cut(s) 340, 541, 1743, 2197
Bst6I CTCTTC 2 cut(s) 743, 2304
BstACI GRCGYC 1 cut(s) 26
BstAFI CTTAAG 3 cut(s) 461, 1235, 2240
BstAUI TGTACA 2 cut(s) 2254, 2283
BstBAI YACGTR 1 cut(s) 1091
BstBI TTCGAA 1 cut(s) 1948
BstC8I GCNNGC 4 cut(s) 231, 984, 1635, 2093
BstDEI CTNAG 6 cut(s) 239, 255, 882, 1038, 1083, 2412
BstF5I GGATG 7 cut(s) 199, 231, 249, 676, 1293, 2251, 2257
BstFNI CGCG 2 cut(s) 1009, 1897
BstKTI GATC 8 cut(s) 10, 141, 268, 592, 628, 967, 1412, 1864
BstMAI GTCTC 1 cut(s) 843
BstMBI GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
BstMWI GCNNNNNNNGC 6 cut(s) 91, 156, 1028, 1147, 1475, 1900
BstNI CCWGG 4 cut(s) 44, 1311, 1508, 1704
BstNSI RCATGY 3 cut(s) 513, 1450, 2095
BstSCI CCNGG 6 cut(s) 42, 161, 272, 1309, 1506, 1702
BstSFI CTRYAG 1 cut(s) 231
BstUI CGCG 2 cut(s) 1009, 1897
BstV1I GCAGC 8 cut(s) 69, 245, 274, 998, 1031, 1402, 1498, 1895
BstV2I GAAGAC 1 cut(s) 487
BstX2I RGATCY 3 cut(s) 7, 625, 1409
BstYI RGATCY 3 cut(s) 7, 625, 1409
BsuI GTATCC 1 cut(s) 2185
BsuRI GGCC 7 cut(s) 814, 840, 1157, 1244, 1511, 1894, 1923
BtgZI GCGATG 1 cut(s) 1024
BtsCI GGATG 7 cut(s) 199, 231, 249, 676, 1293, 2251, 2257
BtsIMutI CAGTG 4 cut(s) 236, 546, 649, 2114
BtuMI TCGCGA 1 cut(s) 1009
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 4 cut(s) 231, 984, 1635, 2093
CaiI CAGNNNCTG 1 cut(s) 629
Cfr13I GGNCC 4 cut(s) 838, 1243, 1542, 2396
CseI GACGC 1 cut(s) 869
Csp6I GTAC 8 cut(s) 127, 308, 532, 617, 772, 1998, 2255, 2284
CviAII CATG 7 cut(s) 199, 510, 1153, 1363, 1447, 1546, 2092
CviQI GTAC 8 cut(s) 127, 308, 532, 617, 772, 1998, 2255, 2284
DdeI CTNAG 6 cut(s) 239, 255, 882, 1038, 1083, 2412
DpnI GATC 8 cut(s) 9, 140, 267, 591, 627, 966, 1411, 1863
DpnII GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
DraI TTTAAA 1 cut(s) 1527
EaeI YGGCCR 2 cut(s) 1155, 1892
Eam1104I CTCTTC 2 cut(s) 743, 2304
EarI CTCTTC 2 cut(s) 743, 2304
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 159, 259
Eco130I CCWWGG 3 cut(s) 809, 1297, 1710
Eco147I AGGCCT 2 cut(s) 814, 1511
Eco24I GRGCYC 4 cut(s) 152, 161, 261, 1081
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 1542, 2396
Eco53kI GAGCTC 2 cut(s) 159, 259
Eco57I CTGAAG 3 cut(s) 74, 990, 2403
Eco72I CACGTG 1 cut(s) 1091
EcoICRI GAGCTC 2 cut(s) 159, 259
EcoO109I RGGNCCY 2 cut(s) 1243, 2396
EcoRII CCWGG 4 cut(s) 42, 1309, 1506, 1702
EcoRV GATATC 1 cut(s) 438
EcoT14I CCWWGG 3 cut(s) 809, 1297, 1710
EcoT22I ATGCAT 2 cut(s) 457, 912
EcoT38I GRGCYC 4 cut(s) 152, 161, 261, 1081
ErhI CCWWGG 3 cut(s) 809, 1297, 1710
FaeI CATG 7 cut(s) 202, 513, 1156, 1366, 1450, 1549, 2095
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 1290, 1322
FaqI GGGAC 2 cut(s) 178, 431
FatI CATG 7 cut(s) 198, 509, 1152, 1362, 1446, 1545, 2091
FauNDI CATATG 1 cut(s) 1905
FbaI TGATCA 2 cut(s) 265, 1861
FblI GTMKAC 1 cut(s) 111
FokI GGATG 7 cut(s) 206, 238, 256, 663, 1280, 2244, 2258
FriOI GRGCYC 4 cut(s) 152, 161, 261, 1081
FspBI CTAG 6 cut(s) 14, 278, 1217, 1634, 1734, 2124
GsaI CCCAGC 1 cut(s) 1760
GsuI CTGGAG 1 cut(s) 2134
HaeIII GGCC 7 cut(s) 814, 840, 1157, 1244, 1511, 1894, 1923
HapII CCGG 4 cut(s) 162, 248, 273, 2028
HgaI GACGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 7 cut(s) 202, 513, 1156, 1366, 1450, 1549, 2095
HincII GTYRAC 2 cut(s) 187, 1068
HindII GTYRAC 2 cut(s) 187, 1068
HinfI GANTC 8 cut(s) 108, 281, 361, 635, 1192, 1351, 1699, 2378
HpaII CCGG 4 cut(s) 162, 248, 273, 2028
HphI GGTGA 2 cut(s) 1832, 2126
Hpy166II GTNNAC 8 cut(s) 112, 187, 673, 772, 859, 1068, 2059, 2372
Hpy188I TCNGA 9 cut(s) 143, 219, 300, 1519, 1622, 1866, 1983, 2383, 2392
Hpy8I GTNNAC 8 cut(s) 112, 187, 673, 772, 859, 1068, 2059, 2372
Hpy99I CGWCG 2 cut(s) 157, 422
HpyAV CCTTC 5 cut(s) 1234, 1256, 1897, 2378, 2387
HpyCH4III ACNGT 4 cut(s) 340, 541, 1743, 2197
HpyCH4IV ACGT 4 cut(s) 26, 420, 1090, 1125
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 156, 1028, 1147, 1475, 1900
HpyF3I CTNAG 6 cut(s) 239, 255, 882, 1038, 1083, 2412
HpySE526I ACGT 4 cut(s) 26, 420, 1090, 1125
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 7 cut(s) 202, 513, 1156, 1366, 1450, 1549, 2095
Ksp22I TGATCA 2 cut(s) 265, 1861
Kzo9I GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
LmnI GCTCC 7 cut(s) 147, 164, 250, 1016, 1084, 1258, 2393
Lsp1109I GCAGC 8 cut(s) 69, 245, 274, 998, 1031, 1402, 1498, 1895
LweI GCATC 9 cut(s) 184, 216, 234, 464, 578, 2061, 2167, 2236, 2363
MaeI CTAG 6 cut(s) 14, 278, 1217, 1634, 1734, 2124
MaeII ACGT 4 cut(s) 26, 420, 1090, 1125
MaeIII GTNAC 6 cut(s) 1091, 1777, 1838, 1912, 1961, 2114
MalI GATC 8 cut(s) 9, 140, 267, 591, 627, 966, 1411, 1863
MboI GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 3 cut(s) 7, 625, 1409
MhlI GDGCHC 5 cut(s) 152, 161, 261, 1033, 1081
MlyI GAGTC 3 cut(s) 117, 275, 2387
MmeI TCCRAC 1 cut(s) 1574
Mph1103I ATGCAT 2 cut(s) 457, 912
MroXI GAANNNNTTC 3 cut(s) 323, 1111, 1328
MseI TTAA 7 cut(s) 39, 462, 1236, 1526, 1668, 1851, 2241
MslI CAYNNNNRTG 4 cut(s) 681, 915, 1550, 1908
MspA1I CMGCKG 1 cut(s) 1022
MspCI CTTAAG 3 cut(s) 461, 1235, 2240
MspI CCGG 4 cut(s) 162, 248, 273, 2028
MspR9I CCNGG 6 cut(s) 44, 163, 274, 1311, 1508, 1704
MunI CAATTG 1 cut(s) 864
Mva1269I GAATGC 3 cut(s) 1202, 1956, 2170
MvaI CCWGG 4 cut(s) 44, 1311, 1508, 1704
MvnI CGCG 2 cut(s) 1009, 1897
MwoI GCNNNNNNNGC 6 cut(s) 91, 156, 1028, 1147, 1475, 1900
NciI CCSGG 2 cut(s) 163, 274
NdeI CATATG 1 cut(s) 1905
NdeII GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
NheI GCTAGC 1 cut(s) 1633
NlaIII CATG 7 cut(s) 202, 513, 1156, 1366, 1450, 1549, 2095
NlaIV GGNNCC 8 cut(s) 62, 149, 252, 1080, 1260, 1629, 1708, 2389
NmuCI GTSAC 5 cut(s) 1091, 1777, 1838, 1961, 2114
NruI TCGCGA 1 cut(s) 1009
NsiI ATGCAT 2 cut(s) 457, 912
NspI RCATGY 3 cut(s) 513, 1450, 2095
NspV TTCGAA 1 cut(s) 1948
PaeI GCATGC 1 cut(s) 2095
PceI AGGCCT 2 cut(s) 814, 1511
PciI ACATGT 1 cut(s) 1446
PctI GAATGC 3 cut(s) 1202, 1956, 2170
PdmI GAANNNNTTC 3 cut(s) 323, 1111, 1328
PfeI GAWTC 5 cut(s) 361, 635, 1192, 1351, 1699
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 2 cut(s) 161, 1309
PleI GAGTC 3 cut(s) 116, 275, 2386
PmaCI CACGTG 1 cut(s) 1091
PmlI CACGTG 1 cut(s) 1091
PpsI GAGTC 3 cut(s) 116, 275, 2386
Ppu21I YACGTR 1 cut(s) 1091
PpuMI RGGWCCY 1 cut(s) 2396
PscI ACATGT 1 cut(s) 1446
PshBI ATTAAT 1 cut(s) 1851
Psp124BI GAGCTC 2 cut(s) 161, 261
Psp5II RGGWCCY 1 cut(s) 2396
Psp6I CCWGG 4 cut(s) 42, 1309, 1506, 1702
PspCI CACGTG 1 cut(s) 1091
PspFI CCCAGC 1 cut(s) 1756
PspGI CCWGG 4 cut(s) 42, 1309, 1506, 1702
PspN4I GGNNCC 8 cut(s) 62, 149, 252, 1080, 1260, 1629, 1708, 2389
PspPI GGNCC 4 cut(s) 838, 1243, 1542, 2396
PspPPI RGGWCCY 1 cut(s) 2396
PsrI GAACNNNNNNTAC 2 cut(s) 1722, 1754
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 3 cut(s) 7, 625, 1409
PsyI GACNNNGTC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 1022
RruI TCGCGA 1 cut(s) 1009
RsaI GTAC 8 cut(s) 128, 309, 533, 618, 773, 1999, 2256, 2285
RsaNI GTAC 8 cut(s) 127, 308, 532, 617, 772, 1998, 2255, 2284
RseI CAYNNNNRTG 4 cut(s) 681, 915, 1550, 1908
SacI GAGCTC 2 cut(s) 161, 261
SaqAI TTAA 7 cut(s) 39, 462, 1236, 1526, 1668, 1851, 2241
Sau3AI GATC 8 cut(s) 7, 138, 265, 589, 625, 964, 1409, 1861
Sau96I GGNCC 4 cut(s) 838, 1243, 1542, 2396
SchI GAGTC 3 cut(s) 117, 275, 2387
ScrFI CCNGG 6 cut(s) 44, 163, 274, 1311, 1508, 1704
SduI GDGCHC 5 cut(s) 152, 161, 261, 1033, 1081
SfaNI GCATC 9 cut(s) 184, 216, 234, 464, 578, 2061, 2167, 2236, 2363
SfcI CTRYAG 1 cut(s) 231
SfuI TTCGAA 1 cut(s) 1948
SinI GGWCC 2 cut(s) 1542, 2396
SmiMI CAYNNNNRTG 4 cut(s) 681, 915, 1550, 1908
SmlI CTYRAG 4 cut(s) 461, 1235, 1490, 2240
SmoI CTYRAG 4 cut(s) 461, 1235, 1490, 2240
SphI GCATGC 1 cut(s) 2095
SseBI AGGCCT 2 cut(s) 814, 1511
SsiI CCGC 7 cut(s) 5, 85, 353, 1141, 1608, 1631, 1895
SspI AATATT 2 cut(s) 1462, 1523
SspMI CTAG 6 cut(s) 14, 278, 1217, 1634, 1734, 2124
SstI GAGCTC 2 cut(s) 161, 261
StuI AGGCCT 2 cut(s) 814, 1511
StyD4I CCNGG 6 cut(s) 42, 161, 272, 1309, 1506, 1702
StyI CCWWGG 3 cut(s) 809, 1297, 1710
TaaI ACNGT 4 cut(s) 340, 541, 1743, 2197
TaiI ACGT 4 cut(s) 29, 423, 1093, 1128
TaqI TCGA 3 cut(s) 434, 654, 1948
TaqII GACCGA 1 cut(s) 1919
TatI WGTACW 6 cut(s) 307, 531, 771, 1997, 2254, 2283
TauI GCSGC 3 cut(s) 88, 1610, 1897
TfiI GAWTC 5 cut(s) 361, 635, 1192, 1351, 1699
Tru1I TTAA 7 cut(s) 39, 462, 1236, 1526, 1668, 1851, 2241
Tru9I TTAA 7 cut(s) 39, 462, 1236, 1526, 1668, 1851, 2241
TscAI CASTG 4 cut(s) 243, 546, 649, 2114
TseFI GTSAC 5 cut(s) 1091, 1777, 1838, 1961, 2114
TseI GCWGC 8 cut(s) 82, 233, 287, 986, 1019, 1415, 1486, 1883
Tsp45I GTSAC 5 cut(s) 1091, 1777, 1838, 1961, 2114
TspDTI ATGAA 8 cut(s) 457, 648, 987, 1423, 1558, 1568, 1712, 1743
TspGWI ACGGA 1 cut(s) 1826
TspRI CASTG 4 cut(s) 243, 546, 649, 2114
Tth111I GACNNNGTC 1 cut(s) 107
Vha464I CTTAAG 3 cut(s) 461, 1235, 2240
VpaK11BI GGWCC 2 cut(s) 1542, 2396
VspI ATTAAT 1 cut(s) 1851
XapI RAATTY 3 cut(s) 1496, 1988, 2224
XbaI TCTAGA 2 cut(s) 13, 2123
XceI RCATGY 3 cut(s) 513, 1450, 2095
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 3 cut(s) 323, 1111, 1328
XspI CTAG 6 cut(s) 14, 278, 1217, 1634, 1734, 2124
ZraI GACGTC 1 cut(s) 27
Zsp2I ATGCAT 2 cut(s) 457, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.