Rw6G028890

CSC1-like protein At4g02900

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010682
Physical Location & Seq
Forward (+)
52944397 .. 52948952
4556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028890.1

Sequence Viewer

Length: 2292 bp
ATGGCGGATCTTCTAGAAATCTTGACGTCAGCAGGTATTAACCTGGCGACAGCATTGGGGTTCCTTGTGGCGTTTGCTTTGCTGCGGCTTCAGCCCATCAACGACAGAGTCTACTTCCCCAAATGGTACCGAAAAGGGATCAGAGGGAGCCCGACGAGCTCCGGGACTTTTGCCAAGAGGTTTGTCAACTTGGATGCCATGACCTACATCAAGTTTCTGAATTGGATGCCTGCAGCACTGAGGATGCCGGAGCCTGAGCTCATTGATCACGCCGGGCTAGACTCTGCTGCCTACATTCGGATTTATGTACTTGGCTTGAAGATATTCTTCCCCATTACTGTTCTTTCACTTGCGGTTTTGATTCCTCTCAATTACACGAACGACACGCTGGAAAAATCCAAGGCAGTAGAATACAGCGACATTGATAAAATCTCGATATCCAATGTCCCTACTGCATCTCTTAAGTTCATTGGACATCTCATATTGTCTTATGTCTTCTCAATTTGGACGTGCTTTGTTTTATACCACGAGTACAAGACAGTGGCTACTAAAAGGCTTCAATTTTTAGCATCTCAAAAACGCCGTCCAGATCAATTTTCGGTCCTTGTGAGGAATGTACCTCCAGATCCTGATGAATCAGTCAGTGAGCATATCGAACACTTCTTTTGCGTAAACCATCCCAACGACTATCTTACACATCAAGTTGTATATAATACAAATAAACTTGCAAAGTTGGTTGCACAGAAGAAGAGTTTGAAAAATAGGTTTGTGTACTACCAAAATAAGTACGAGAGAAATCCAGAGAAATCAAGGCCTACTACAAAGACAGGTTTCTTGGGCCTGTTGGGAGACAAAGTGGACGCAATTGATTACTACACAACTGAGATTGAAAAGTTGAGCGAAGAAGAGAAAAAAGAAAGAGAAAGAGTTGCGAGTGATCCTGAAGCCATAATGCCAGCAGCATTTGTTTCATTCAAATCGCGATGGGGAGCAGCTGTATGTGCTCAAACTCAGCAATCAAGTAATGCTACCATCTGGTTGACAGAGTGGGCTCCTGAGCCACGTGACATCTATTGGAGAAATCTTTCAATTCCATACGTTGAACTCAATGTGCGGAAACTGCTCATGGCTGTTGCTTTCTTTGTTCTCATTTCCTTTTTTATGATTCCAATAGCATTCGTCCAAACTCTAGCCAACATTGACAATCTTACGAAGGCCCTTCCTTTCTTGAAGCCAATAATAGAGAAGAAGCAAGTTAAGTCTGTCATCCAAGGATTTCTTCCAGGAATTGCATTGAAGATATTCCTTGCTATACTTCCTATGATTCTAATGCTCATGTCAAAGATAGAGGGTTATACATCACTCTCATCTCTGGATAGGAGATCTGCTGCGAAGTATTATTTATTCATACTTATCAATATGTTTCTGGGAAATATTGTTGCAGGAACAGCATTTGAGCAGCTTGAGGAATTTACTAGCCAGGCCTCATCAGAATATTTAAAAACTATTGGGAGGACCATGCCAATGAAAGGCACATTTTTCATCACTTATATAATGGTTGATGGTTGGAGTGGAATTGCTGCAGAAATCATCAGATTGGTTCCGCTAGCTCTATACCACTTCAAAAACACATTTTTGGTTAAGACAGAACAAGATAGGGAGGAAGCAATGAATCCTGGTTCCTTGGCATTTGCTACAAATGAACCTAGAATACAGTTCTATATGTTGCTGGGCCTTGTATATTCTGTTGTCACTCCTTTACTTCTTCCATTTATTCTTATCTTCTTTGCTTTTGCCTACGTGGTTTTCCGTCACCAGATTATTAATGTCTATGATCAGAAATATGAGAGTGGTGCAGCCTTTTGGCCGCGTGTGCATATGCGTGTAATTATTGGCCTAATCGTATCCCAAGTCCTTGTAATTGGACTATTTAGCACATTAGATGTGCACTATTCAACATATATTCTCATTCCACAACCTATTTTGACATTCTGGTTCTATAGAGTCTGCAAGGGTCGTTTCGAACCAGCATTCGTGACATTTCCTTTACAGGATGCAATGGTAAAGGATACAGTTGAACGAGCTACTGAACCAACCTTGAATTTGTTGGCCTATCTTAAGGATGCTTATGTACATCCTGACTTCAAGGGAGGGGAGATGTACAAACCCGAAGCCATTGACGAAGAAGAGAGAAATCCTCTTGTTCCTACAAAGAGAACATCTCAAATGGCATCTCAAACTGGTAGTAAACACGAGTCTGAAGGCTCTGAAGGACCTTTTTTGAAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

763

Amino Acids

87.05

Weight (kDa)

8.84

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 7 - 177 1.7e-38 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 198 - 361 5.6e-49 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 372 - 644 4.1e-85 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010682)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02900 AT4G02900 AT4G02900 AT4G02900
fragaria_vesca FvH4_2g23940 FvH4_2g23940
malus_domestica MD11G1289500.v1.1
prunus_persica Prupe.8G244700_v2.0.a1 Prupe.8G244700_v2.0.a1
pyrus_communis pycom11g25690
rosa_chinensis RchiOBHm_Chr6g0291571
rosa_laevigata RLG00000012106
rosa_roxburghii Rroxscaffold_7G00175960
rosa_rugosa Rorug06G0220300 Rorug06G0220400 Rorug06G0220400
rosa_samantha Rh6AG332700 Rh6CG345900 Rh6DG333100
rosa_wichuraiana Rw6G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 29
Acc36I ACCTGC 1 cut(s) 23
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 111
AccII CGCG 2 cut(s) 982, 1870
AciI CCGC 6 cut(s) 5, 85, 353, 1114, 1604, 1868
AclWI GGATC 4 cut(s) 15, 146, 620, 932
AcoI YGGCCR 1 cut(s) 1865
AcsI RAATTY 2 cut(s) 1469, 2101
AcuI CTGAAG 4 cut(s) 74, 963, 2280, 2289
AcvI CACGTG 1 cut(s) 1064
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 8 cut(s) 128, 309, 533, 618, 773, 788, 2133, 2162
AfiI CCNNNNNNNGG 2 cut(s) 297, 1529
AflII CTTAAG 2 cut(s) 461, 2117
AjiI CACGTC 1 cut(s) 510
AjnI CCWGG 4 cut(s) 42, 1282, 1479, 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
AluBI AGCT 6 cut(s) 159, 259, 995, 1462, 1610, 2084
AluI AGCT 6 cut(s) 159, 259, 995, 1462, 1610, 2084
Alw21I GWGCWC 4 cut(s) 161, 261, 1006, 1950
Alw26I GTCTC 1 cut(s) 843
Alw44I GTGCAC 1 cut(s) 1946
AlwI GGATC 4 cut(s) 15, 146, 620, 932
AlwNI CAGNNNCTG 1 cut(s) 629
AoxI GGCC 8 cut(s) 812, 838, 1215, 1482, 1732, 1865, 1894, 2109
ApaLI GTGCAC 1 cut(s) 1946
ApeKI GCWGC 9 cut(s) 82, 233, 287, 959, 992, 1388, 1459, 1580, 1856
ApoI RAATTY 2 cut(s) 1469, 2101
ArsI GACNNNNNNTTYG 2 cut(s) 568, 600
AseI ATTAAT 1 cut(s) 1824
Asp700I GAANNNNTTC 3 cut(s) 323, 1084, 1301
Asp718I GGTACC 1 cut(s) 126
AspS9I GGNCC 6 cut(s) 601, 838, 1216, 1515, 1732, 2273
AsuC2I CCSGG 2 cut(s) 163, 274
AsuHPI GGTGA 1 cut(s) 1805
AsuII TTCGAA 1 cut(s) 2022
AsuNHI GCTAGC 1 cut(s) 1606
AvaII GGWCC 3 cut(s) 601, 1515, 2273
BaeGI GKGCMC 1 cut(s) 1950
BanI GGYRCC 1 cut(s) 126
BanII GRGCYC 4 cut(s) 152, 161, 261, 1054
BauI CACGAG 2 cut(s) 527, 2252
BbrPI CACGTG 1 cut(s) 1064
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 4 cut(s) 161, 261, 1006, 1950
BbvI GCAGC 9 cut(s) 69, 245, 274, 971, 1004, 1375, 1471, 1567, 1868
BccI CCATC 5 cut(s) 104, 684, 978, 1040, 1556
BceAI ACGGC 1 cut(s) 567
BciT130I CCWGG 4 cut(s) 44, 1284, 1481, 1677
BciVI GTATCC 2 cut(s) 1915, 2062
BclI TGATCA 2 cut(s) 265, 1834
BcnI CCSGG 2 cut(s) 163, 274
BcoDI GTCTC 1 cut(s) 843
BfaI CTAG 6 cut(s) 14, 278, 1190, 1476, 1607, 1707
BfmI CTRYAG 3 cut(s) 231, 1581, 1999
BfrI CTTAAG 2 cut(s) 461, 2117
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 2 cut(s) 1915, 2062
BglII AGATCT 1 cut(s) 1382
Bme1390I CCNGG 6 cut(s) 44, 163, 274, 1284, 1481, 1677
Bme18I GGWCC 3 cut(s) 601, 1515, 2273
BmgBI CACGTC 1 cut(s) 510
BmgT120I GGNCC 6 cut(s) 601, 838, 1216, 1515, 1732, 2273
BmiI GGNNCC 7 cut(s) 62, 128, 149, 252, 1053, 1602, 1681
BmrFI CCNGG 6 cut(s) 44, 163, 274, 1284, 1481, 1677
BmsI GCATC 8 cut(s) 184, 216, 234, 464, 578, 2044, 2113, 2240
BmtI GCTAGC 1 cut(s) 1610
BpiI GAAGAC 1 cut(s) 487
BplI GAGNNNNNCTC 4 cut(s) 2182, 2214, 2206, 2238
BpmI CTGGAG 1 cut(s) 606
Bpu10I CCTNAGC 2 cut(s) 255, 1056
Bpu14I TTCGAA 1 cut(s) 2022
BpuEI CTTGAG 1 cut(s) 1484
BpuMI CCSGG 2 cut(s) 163, 274
BsaAI YACGTR 2 cut(s) 1064, 1801
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 3 cut(s) 399, 1270, 1683
BsaXI ACNNNNNCTCC 4 cut(s) 840, 870, 981, 1011
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 1529
Bse1I ACTGG 1 cut(s) 2245
Bse3DI GCAATG 2 cut(s) 1674, 2064
BseBI CCWGG 4 cut(s) 44, 1284, 1481, 1677
BseDI CCNNGG 3 cut(s) 399, 1270, 1683
BseGI GGATG 8 cut(s) 199, 231, 249, 676, 1266, 2059, 2128, 2134
BseLI CCNNNNNNNGG 2 cut(s) 297, 1529
BseMI GCAATG 2 cut(s) 1674, 2064
BseMII CTCAG 5 cut(s) 230, 246, 873, 1025, 1047
BseNI ACTGG 1 cut(s) 2245
BseSI GKGCMC 1 cut(s) 1950
BseXI GCAGC 9 cut(s) 69, 245, 274, 971, 1004, 1375, 1471, 1567, 1868
BseYI CCCAGC 1 cut(s) 1729
BsgI GTGCAG 1 cut(s) 1875
Bsh1236I CGCG 2 cut(s) 982, 1870
BshFI GGCC 8 cut(s) 814, 840, 1217, 1484, 1734, 1867, 1896, 2111
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 4 cut(s) 161, 261, 1006, 1950
BsiSI CCGG 3 cut(s) 162, 248, 273
BslFI GGGAC 2 cut(s) 178, 431
BslI CCNNNNNNNGG 2 cut(s) 297, 1529
BsmAI GTCTC 1 cut(s) 843
BsmFI GGGAC 2 cut(s) 178, 431
BsmI GAATGC 2 cut(s) 1175, 2030
BsnI GGCC 8 cut(s) 814, 840, 1217, 1484, 1734, 1867, 1896, 2111
Bsp119I TTCGAA 1 cut(s) 2022
Bsp1286I GDGCHC 6 cut(s) 152, 161, 261, 1006, 1054, 1950
Bsp1407I TGTACA 2 cut(s) 2131, 2160
Bsp143I GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
Bsp68I TCGCGA 1 cut(s) 982
BspACI CCGC 6 cut(s) 5, 85, 353, 1114, 1604, 1868
BspANI GGCC 8 cut(s) 814, 840, 1217, 1484, 1734, 1867, 1896, 2111
BspCNI CTCAG 5 cut(s) 231, 247, 874, 1024, 1048
BspFNI CGCG 2 cut(s) 982, 1870
BspLI GGNNCC 7 cut(s) 62, 128, 149, 252, 1053, 1602, 1681
BspMAI CTGCAG 2 cut(s) 235, 1585
BspMI ACCTGC 1 cut(s) 23
BspOI GCTAGC 1 cut(s) 1610
BspPI GGATC 4 cut(s) 15, 146, 620, 932
BspT104I TTCGAA 1 cut(s) 2022
BspT107I GGYRCC 1 cut(s) 126
BspTI CTTAAG 2 cut(s) 461, 2117
BsrDI GCAATG 2 cut(s) 1674, 2064
BsrGI TGTACA 2 cut(s) 2131, 2160
BsrI ACTGG 1 cut(s) 2245
BssECI CCNNGG 3 cut(s) 399, 1270, 1683
BssMI GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 2 cut(s) 527, 2252
BssT1I CCWWGG 3 cut(s) 399, 1270, 1683
Bst2BI CACGAG 2 cut(s) 527, 2252
Bst2UI CCWGG 4 cut(s) 44, 1284, 1481, 1677
Bst4CI ACNGT 4 cut(s) 340, 541, 1716, 2074
Bst6I CTCTTC 3 cut(s) 743, 900, 2181
BstACI GRCGYC 1 cut(s) 26
BstAFI CTTAAG 2 cut(s) 461, 2117
BstAUI TGTACA 2 cut(s) 2131, 2160
BstBAI YACGTR 2 cut(s) 1064, 1801
BstBI TTCGAA 1 cut(s) 2022
BstC8I GCNNGC 3 cut(s) 231, 957, 1608
BstDEI CTNAG 6 cut(s) 239, 255, 882, 1011, 1056, 2289
BstF5I GGATG 8 cut(s) 199, 231, 249, 676, 1266, 2059, 2128, 2134
BstFNI CGCG 2 cut(s) 982, 1870
BstKTI GATC 8 cut(s) 10, 141, 268, 592, 628, 940, 1385, 1837
BstMAI GTCTC 1 cut(s) 843
BstMBI GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
BstMWI GCNNNNNNNGC 6 cut(s) 91, 156, 1001, 1120, 1448, 1873
BstNI CCWGG 4 cut(s) 44, 1284, 1481, 1677
BstSCI CCNGG 6 cut(s) 42, 161, 272, 1282, 1479, 1675
BstSFI CTRYAG 3 cut(s) 231, 1581, 1999
BstSLI GKGCMC 1 cut(s) 1950
BstUI CGCG 2 cut(s) 982, 1870
BstV1I GCAGC 9 cut(s) 69, 245, 274, 971, 1004, 1375, 1471, 1567, 1868
BstV2I GAAGAC 1 cut(s) 487
BstX2I RGATCY 3 cut(s) 7, 625, 1382
BstYI RGATCY 3 cut(s) 7, 625, 1382
BsuI GTATCC 2 cut(s) 1915, 2062
BsuRI GGCC 8 cut(s) 814, 840, 1217, 1484, 1734, 1867, 1896, 2111
BtgZI GCGATG 1 cut(s) 997
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 8 cut(s) 199, 231, 249, 676, 1266, 2059, 2128, 2134
BtsIMutI CAGTG 3 cut(s) 236, 546, 649
BtuMI TCGCGA 1 cut(s) 982
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 3 cut(s) 231, 957, 1608
CaiI CAGNNNCTG 1 cut(s) 629
Cfr13I GGNCC 6 cut(s) 601, 838, 1216, 1515, 1732, 2273
CseI GACGC 1 cut(s) 869
Csp6I GTAC 8 cut(s) 127, 308, 532, 617, 772, 787, 2132, 2161
CviAII CATG 4 cut(s) 199, 1126, 1336, 1519
CviQI GTAC 8 cut(s) 127, 308, 532, 617, 772, 787, 2132, 2161
DdeI CTNAG 6 cut(s) 239, 255, 882, 1011, 1056, 2289
DpnI GATC 8 cut(s) 9, 140, 267, 591, 627, 939, 1384, 1836
DpnII GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
DraI TTTAAA 1 cut(s) 1500
EaeI YGGCCR 1 cut(s) 1865
Eam1104I CTCTTC 3 cut(s) 743, 900, 2181
EarI CTCTTC 3 cut(s) 743, 900, 2181
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 159, 259
Eco130I CCWWGG 3 cut(s) 399, 1270, 1683
Eco147I AGGCCT 2 cut(s) 814, 1484
Eco24I GRGCYC 4 cut(s) 152, 161, 261, 1054
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 3 cut(s) 601, 1515, 2273
Eco53kI GAGCTC 2 cut(s) 159, 259
Eco57I CTGAAG 4 cut(s) 74, 963, 2280, 2289
Eco72I CACGTG 1 cut(s) 1064
EcoICRI GAGCTC 2 cut(s) 159, 259
EcoO109I RGGNCCY 2 cut(s) 1216, 2273
EcoRII CCWGG 4 cut(s) 42, 1282, 1479, 1675
EcoRV GATATC 1 cut(s) 438
EcoT14I CCWWGG 3 cut(s) 399, 1270, 1683
EcoT38I GRGCYC 4 cut(s) 152, 161, 261, 1054
ErhI CCWWGG 3 cut(s) 399, 1270, 1683
FaeI CATG 4 cut(s) 202, 1129, 1339, 1522
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 1263, 1295
FaqI GGGAC 2 cut(s) 178, 431
FatI CATG 4 cut(s) 198, 1125, 1335, 1518
FauNDI CATATG 1 cut(s) 1878
FbaI TGATCA 2 cut(s) 265, 1834
FblI GTMKAC 1 cut(s) 111
FokI GGATG 8 cut(s) 206, 238, 256, 663, 1253, 2066, 2121, 2135
FriOI GRGCYC 4 cut(s) 152, 161, 261, 1054
FspBI CTAG 6 cut(s) 14, 278, 1190, 1476, 1607, 1707
GsaI CCCAGC 1 cut(s) 1733
GsuI CTGGAG 1 cut(s) 606
HaeIII GGCC 8 cut(s) 814, 840, 1217, 1484, 1734, 1867, 1896, 2111
HapII CCGG 3 cut(s) 162, 248, 273
HgaI GACGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 4 cut(s) 202, 1129, 1339, 1522
HincII GTYRAC 2 cut(s) 187, 1041
HindII GTYRAC 2 cut(s) 187, 1041
HinfI GANTC 9 cut(s) 108, 281, 361, 635, 1165, 1324, 1672, 2004, 2255
HpaII CCGG 3 cut(s) 162, 248, 273
HphI GGTGA 1 cut(s) 1805
Hpy166II GTNNAC 8 cut(s) 112, 187, 673, 772, 859, 1041, 1948, 2249
Hpy188I TCNGA 8 cut(s) 143, 219, 300, 1492, 1595, 1839, 2260, 2269
Hpy8I GTNNAC 8 cut(s) 112, 187, 673, 772, 859, 1041, 1948, 2249
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 4 cut(s) 1207, 1229, 2255, 2264
HpyCH4III ACNGT 4 cut(s) 340, 541, 1716, 2074
HpyCH4IV ACGT 5 cut(s) 26, 509, 1063, 1098, 1800
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 156, 1001, 1120, 1448, 1873
HpyF3I CTNAG 6 cut(s) 239, 255, 882, 1011, 1056, 2289
HpySE526I ACGT 5 cut(s) 26, 509, 1063, 1098, 1800
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 4 cut(s) 202, 1129, 1339, 1522
KpnI GGTACC 1 cut(s) 130
Ksp22I TGATCA 2 cut(s) 265, 1834
Kzo9I GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
LmnI GCTCC 5 cut(s) 147, 164, 250, 989, 1057
Lsp1109I GCAGC 9 cut(s) 69, 245, 274, 971, 1004, 1375, 1471, 1567, 1868
LweI GCATC 8 cut(s) 184, 216, 234, 464, 578, 2044, 2113, 2240
MaeI CTAG 6 cut(s) 14, 278, 1190, 1476, 1607, 1707
MaeII ACGT 5 cut(s) 26, 509, 1063, 1098, 1800
MaeIII GTNAC 4 cut(s) 1064, 1750, 1811, 2035
MalI GATC 8 cut(s) 9, 140, 267, 591, 627, 939, 1384, 1836
MboI GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 3 cut(s) 7, 625, 1382
MhlI GDGCHC 6 cut(s) 152, 161, 261, 1006, 1054, 1950
MlyI GAGTC 4 cut(s) 117, 275, 2013, 2264
MmeI TCCRAC 1 cut(s) 1547
MroXI GAANNNNTTC 3 cut(s) 323, 1084, 1301
MseI TTAA 7 cut(s) 39, 462, 1257, 1499, 1641, 1824, 2118
MslI CAYNNNNRTG 2 cut(s) 1523, 1881
MspA1I CMGCKG 1 cut(s) 995
MspCI CTTAAG 2 cut(s) 461, 2117
MspI CCGG 3 cut(s) 162, 248, 273
MspR9I CCNGG 6 cut(s) 44, 163, 274, 1284, 1481, 1677
MunI CAATTG 1 cut(s) 864
Mva1269I GAATGC 2 cut(s) 1175, 2030
MvaI CCWGG 4 cut(s) 44, 1284, 1481, 1677
MvnI CGCG 2 cut(s) 982, 1870
MwoI GCNNNNNNNGC 6 cut(s) 91, 156, 1001, 1120, 1448, 1873
NciI CCSGG 2 cut(s) 163, 274
NdeI CATATG 1 cut(s) 1878
NdeII GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
NheI GCTAGC 1 cut(s) 1606
NlaIII CATG 4 cut(s) 202, 1129, 1339, 1522
NlaIV GGNNCC 7 cut(s) 62, 128, 149, 252, 1053, 1602, 1681
NmuCI GTSAC 4 cut(s) 1064, 1750, 1811, 2035
NruI TCGCGA 1 cut(s) 982
NspV TTCGAA 1 cut(s) 2022
PceI AGGCCT 2 cut(s) 814, 1484
PctI GAATGC 2 cut(s) 1175, 2030
PdmI GAANNNNTTC 3 cut(s) 323, 1084, 1301
PfeI GAWTC 5 cut(s) 361, 635, 1165, 1324, 1672
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 2 cut(s) 161, 1282
PleI GAGTC 4 cut(s) 116, 275, 2012, 2263
PmaCI CACGTG 1 cut(s) 1064
PmlI CACGTG 1 cut(s) 1064
PpsI GAGTC 4 cut(s) 116, 275, 2012, 2263
Ppu21I YACGTR 2 cut(s) 1064, 1801
PpuMI RGGWCCY 1 cut(s) 2273
PshBI ATTAAT 1 cut(s) 1824
Psp124BI GAGCTC 2 cut(s) 161, 261
Psp5II RGGWCCY 1 cut(s) 2273
Psp6I CCWGG 4 cut(s) 42, 1282, 1479, 1675
PspCI CACGTG 1 cut(s) 1064
PspFI CCCAGC 1 cut(s) 1729
PspGI CCWGG 4 cut(s) 42, 1282, 1479, 1675
PspN4I GGNNCC 7 cut(s) 62, 128, 149, 252, 1053, 1602, 1681
PspPI GGNCC 6 cut(s) 601, 838, 1216, 1515, 1732, 2273
PspPPI RGGWCCY 1 cut(s) 2273
PsrI GAACNNNNNNTAC 2 cut(s) 1695, 1727
PstI CTGCAG 2 cut(s) 235, 1585
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 3 cut(s) 7, 625, 1382
PsyI GACNNNGTC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 995
RruI TCGCGA 1 cut(s) 982
RsaI GTAC 8 cut(s) 128, 309, 533, 618, 773, 788, 2133, 2162
RsaNI GTAC 8 cut(s) 127, 308, 532, 617, 772, 787, 2132, 2161
RseI CAYNNNNRTG 2 cut(s) 1523, 1881
SacI GAGCTC 2 cut(s) 161, 261
SaqAI TTAA 7 cut(s) 39, 462, 1257, 1499, 1641, 1824, 2118
Sau3AI GATC 8 cut(s) 7, 138, 265, 589, 625, 937, 1382, 1834
Sau96I GGNCC 6 cut(s) 601, 838, 1216, 1515, 1732, 2273
SchI GAGTC 4 cut(s) 117, 275, 2013, 2264
ScrFI CCNGG 6 cut(s) 44, 163, 274, 1284, 1481, 1677
SduI GDGCHC 6 cut(s) 152, 161, 261, 1006, 1054, 1950
SfaNI GCATC 8 cut(s) 184, 216, 234, 464, 578, 2044, 2113, 2240
SfcI CTRYAG 3 cut(s) 231, 1581, 1999
SfuI TTCGAA 1 cut(s) 2022
SinI GGWCC 3 cut(s) 601, 1515, 2273
SmiMI CAYNNNNRTG 2 cut(s) 1523, 1881
SmlI CTYRAG 3 cut(s) 461, 1463, 2117
SmoI CTYRAG 3 cut(s) 461, 1463, 2117
SseBI AGGCCT 2 cut(s) 814, 1484
SsiI CCGC 6 cut(s) 5, 85, 353, 1114, 1604, 1868
SspI AATATT 2 cut(s) 1435, 1496
SspMI CTAG 6 cut(s) 14, 278, 1190, 1476, 1607, 1707
SstI GAGCTC 2 cut(s) 161, 261
StuI AGGCCT 2 cut(s) 814, 1484
StyD4I CCNGG 6 cut(s) 42, 161, 272, 1282, 1479, 1675
StyI CCWWGG 3 cut(s) 399, 1270, 1683
TaaI ACNGT 4 cut(s) 340, 541, 1716, 2074
TaiI ACGT 5 cut(s) 29, 512, 1066, 1101, 1803
TaqI TCGA 3 cut(s) 434, 654, 2022
TaqII GACCGA 1 cut(s) 589
TatI WGTACW 5 cut(s) 307, 531, 771, 2131, 2160
TauI GCSGC 2 cut(s) 88, 1870
TfiI GAWTC 5 cut(s) 361, 635, 1165, 1324, 1672
Tru1I TTAA 7 cut(s) 39, 462, 1257, 1499, 1641, 1824, 2118
Tru9I TTAA 7 cut(s) 39, 462, 1257, 1499, 1641, 1824, 2118
TscAI CASTG 3 cut(s) 243, 546, 649
TseFI GTSAC 4 cut(s) 1064, 1750, 1811, 2035
TseI GCWGC 9 cut(s) 82, 233, 287, 959, 992, 1388, 1459, 1580, 1856
Tsp45I GTSAC 4 cut(s) 1064, 1750, 1811, 2035
TspDTI ATGAA 8 cut(s) 457, 648, 960, 1396, 1531, 1541, 1685, 1716
TspGWI ACGGA 1 cut(s) 1799
TspRI CASTG 3 cut(s) 243, 546, 649
Tth111I GACNNNGTC 1 cut(s) 107
Vha464I CTTAAG 2 cut(s) 461, 2117
VneI GTGCAC 1 cut(s) 1946
VpaK11BI GGWCC 3 cut(s) 601, 1515, 2273
VspI ATTAAT 1 cut(s) 1824
XapI RAATTY 2 cut(s) 1469, 2101
XbaI TCTAGA 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 3 cut(s) 323, 1084, 1301
XspI CTAG 6 cut(s) 14, 278, 1190, 1476, 1607, 1707
ZraI GACGTC 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.