Rroxscaffold_7G00182920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017244
Physical Location & Seq
Reverse (-)
22004734 .. 22005989
1256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182920.1

Sequence Viewer

Length: 1182 bp
ATGTTGCTACAGAATGAACGAGTAGGAGAGGATCCTATTCGTCAGTCAGTGCACCGCAGGCATCCTCGATATTCATCCATTGAAGAAAGATCAGAATCAAAATTAATTCCTATGAATATTGATGATCTGCCTGGCCTTGTATTGGTTGAGATTCTTTATAGACTTCCTTGCTATAAACTTGTTTTTCAGTCCCAGTGTGTCTCCAAGCGTTGTTGCGCTCTCTTCTCAGATCCTTACTTTATTGGCCGCTTTATATGCCATCTCCGAAGTAGTGATATGGAAGCGCCACCAATAATCCGTACTCTGATAAACCGCAAAGGGGAGGAACTGTCATGGTCACCATCGTGGTGGTCGTCCAATTTGCTAGCACCGGTATTCAGAGAACTCATGAGTTTCCATGGATTGAAATATGAGCCAATTGTGAAGGCGACTTATAATGACTTGGTTTTGTGCTGCGAAGACGGGATGGACTTCTACTACATTCAACTGTGGAATGGGTTGCTCTTCCTCCCTCTCCTCGGTCTTATACTTGGCCACAAAGGAAAGCACCAGCAGCAATCATCTGTGATCGTTGTCAGAATACTTCCATTTGTAGGAGAGGATCCACATTATCAACCCTCCAGGTTTACTGTGGAGATCTTTTCTTCCGAGACTGGTGAGTGGAAAGAGTCGGTCGTAGCTTCCCCTGTACCTTTGAATTTGAGCACAATGCGTATTAGCTTTGCTTACAATGGAATGTTGTATTGGTGGGATTCTTTAACCAACCGATATGTTGGGATGGATCCTTTTATGATCAACAGTAATGGTAATGGTGATCATTATAAACTTCGTTTCATGAAATTTGATGATGAAGTTTTAGATCACCTTGGCACCATTAACTGCTTTGGTGTGTGCGGAGGGTGTCTACGTATGTGTTACTACCAGGAGGCAGACGATACTCTATATGTTTGGGACTGGAAAGAAGAACAAGATTGTGATCAGAATGGAGCTGGCAGCAGTGAATTGTGTTGGAAACAGACTGGAGTCTATTCACTGGGATTGAGAATGGCTTCTGTTGGGGAATTGTTAGCTTTTGATCCAAATAATGATGACATGTTGTATCTTGATGTTGATGAAGAAATTTGCCTGTGCAACATTCAGAACGGGAAATTTGTCAGAGCTCGCTGGAAAGAGTCACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

393

Amino Acids

45.49

Weight (kDa)

5.22

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 204 - 385 1.7e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017244)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19900 FvH4_2g19920
prunus_persica Prupe.1G510400_v2.0.a1
pyrus_communis pycom05g29490
rosa_chinensis RchiOBHm_Chr6g0285591
rosa_laevigata RLG00000012624
rosa_multiflora Rmu_co8292461.1_g000001 Rmu_sc0017770.1_g000007
rosa_roxburghii Rroxscaffold_7G00182920
rosa_rugosa Rorug06G0175900
rosa_samantha Rh6BG288600 Rh6BG288700 Rh6CG289800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 435, 822
AccB1I GGYRCC 1 cut(s) 869
AccI GTMKAC 1 cut(s) 904
AciI CCGC 4 cut(s) 55, 247, 313, 894
AclWI GGATC 8 cut(s) 26, 39, 224, 596, 609, 776, 789, 1070
AcoI YGGCCR 2 cut(s) 244, 532
AcsI RAATTY 4 cut(s) 697, 839, 1119, 1148
AfaI GTAC 2 cut(s) 301, 690
AfiI CCNNNNNNNGG 4 cut(s) 142, 319, 518, 593
AflIII ACRYGT 1 cut(s) 1092
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 4 cut(s) 83, 406, 485, 697
AjnI CCWGG 3 cut(s) 130, 620, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 727, 759
AleI CACNNNNGTG 1 cut(s) 343
AluBI AGCT 5 cut(s) 680, 720, 989, 1070, 1160
AluI AGCT 5 cut(s) 680, 720, 989, 1070, 1160
Alw21I GWGCWC 3 cut(s) 54, 707, 1162
Alw26I GTCTC 2 cut(s) 205, 644
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 8 cut(s) 26, 39, 224, 596, 609, 776, 789, 1070
AoxI GGCC 3 cut(s) 133, 244, 532
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 453, 553, 993
ApoI RAATTY 4 cut(s) 697, 839, 1119, 1148
AseI ATTAAT 1 cut(s) 104
AsiGI ACCGGT 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 1048
AspLEI GCGC 2 cut(s) 218, 286
AsuHPI GGTGA 4 cut(s) 330, 668, 824, 854
AsuNHI GCTAGC 1 cut(s) 364
BaeGI GKGCMC 1 cut(s) 54
BalI TGGCCA 1 cut(s) 534
BamHI GGATCC 3 cut(s) 31, 601, 781
BanI GGYRCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 1162
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 3 cut(s) 54, 707, 1162
BbvI GCAGC 3 cut(s) 440, 565, 1005
BccI CCATC 4 cut(s) 267, 349, 460, 772
BciT130I CCWGG 3 cut(s) 132, 622, 923
BclI TGATCA 3 cut(s) 792, 814, 976
BcoDI GTCTC 2 cut(s) 205, 644
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 8
BfoI RGCGCY 1 cut(s) 287
BglII AGATCT 1 cut(s) 636
BisI GCNGC 4 cut(s) 247, 454, 554, 994
BlsI GCNGC 4 cut(s) 248, 455, 555, 995
Bme1390I CCNGG 3 cut(s) 132, 622, 923
BmiI GGNNCC 4 cut(s) 33, 603, 783, 871
BmrFI CCNGG 3 cut(s) 132, 622, 923
BmrI ACTGGG 2 cut(s) 187, 1043
BmsI GCATC 1 cut(s) 70
BmtI GCTAGC 1 cut(s) 368
BmuI ACTGGG 2 cut(s) 187, 1043
BoxI GACNNNNGTC 1 cut(s) 1022
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 2 cut(s) 604, 1041
BsaAI YACGTR 1 cut(s) 908
BsaBI GATNNNNATC 3 cut(s) 73, 94, 975
BsaJI CCNNGG 3 cut(s) 397, 517, 865
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 319, 518, 593
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 5 cut(s) 193, 658, 959, 1024, 1038
Bse8I GATNNNNATC 3 cut(s) 73, 94, 975
BseBI CCWGG 3 cut(s) 132, 622, 923
BseDI CCNNGG 3 cut(s) 397, 517, 865
BseGI GGATG 4 cut(s) 61, 74, 471, 783
BseJI GATNNNNATC 3 cut(s) 73, 94, 975
BseLI CCNNNNNNNGG 4 cut(s) 142, 319, 518, 593
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 5 cut(s) 193, 658, 959, 1024, 1038
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 3 cut(s) 440, 565, 1005
Bsh1285I CGRYCG 1 cut(s) 675
BshFI GGCC 3 cut(s) 135, 246, 534
BshNI GGYRCC 1 cut(s) 869
BshTI ACCGGT 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 675
BsiHKAI GWGCWC 3 cut(s) 54, 707, 1162
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 2 cut(s) 175, 965
BslI CCNNNNNNNGG 4 cut(s) 142, 319, 518, 593
BsmAI GTCTC 2 cut(s) 205, 644
BsmFI GGGAC 2 cut(s) 175, 965
BsnI GGCC 3 cut(s) 135, 246, 534
Bsp1286I GDGCHC 3 cut(s) 54, 707, 1162
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 4 cut(s) 55, 247, 313, 894
BspANI GGCC 3 cut(s) 135, 246, 534
BspCNI CTCAG 1 cut(s) 239
BspHI TCATGA 2 cut(s) 387, 834
BspLI GGNNCC 4 cut(s) 33, 603, 783, 871
BspOI GCTAGC 1 cut(s) 368
BspPI GGATC 8 cut(s) 26, 39, 224, 596, 609, 776, 789, 1070
BspQI GCTCTTC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 869
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 5 cut(s) 193, 658, 959, 1024, 1038
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 3 cut(s) 397, 517, 865
BssT1I CCWWGG 2 cut(s) 397, 865
Bst2UI CCWGG 3 cut(s) 132, 622, 923
Bst4CI ACNGT 4 cut(s) 330, 489, 631, 800
Bst6I CTCTTC 2 cut(s) 227, 509
BstBAI YACGTR 1 cut(s) 908
BstC8I GCNNGC 4 cut(s) 59, 366, 991, 1162
BstDEI CTNAG 1 cut(s) 226
BstDSI CCRYGG 1 cut(s) 397
BstEII GGTNACC 1 cut(s) 336
BstF5I GGATG 4 cut(s) 61, 74, 471, 783
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 2 cut(s) 218, 286
BstMAI GTCTC 2 cut(s) 205, 644
BstMCI CGRYCG 1 cut(s) 675
BstMWI GCNNNNNNNGC 3 cut(s) 58, 255, 553
BstNI CCWGG 3 cut(s) 132, 622, 923
BstNSI RCATGY 1 cut(s) 1096
BstPAI GACNNNNGTC 1 cut(s) 1022
BstPI GGTNACC 1 cut(s) 336
BstSCI CCNGG 3 cut(s) 130, 620, 921
BstSFI CTRYAG 1 cut(s) 8
BstSLI GKGCMC 1 cut(s) 54
BstSNI TACGTA 1 cut(s) 908
BstV1I GCAGC 3 cut(s) 440, 565, 1005
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 5 cut(s) 31, 229, 601, 636, 781
BstXI CCANNNNNNTGG 1 cut(s) 348
BstYI RGATCY 5 cut(s) 31, 229, 601, 636, 781
BsuRI GGCC 3 cut(s) 135, 246, 534
BtgI CCRYGG 1 cut(s) 397
BtsCI GGATG 4 cut(s) 61, 74, 471, 783
BtsI GCAGTG 1 cut(s) 1003
BtsIMutI CAGTG 4 cut(s) 54, 200, 1003, 1031
Cac8I GCNNGC 4 cut(s) 59, 366, 991, 1162
CciI TCATGA 2 cut(s) 387, 834
CfoI GCGC 2 cut(s) 218, 286
Cfr10I RCCGGY 1 cut(s) 370
Csp6I GTAC 2 cut(s) 300, 689
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 5 cut(s) 333, 388, 398, 835, 1093
CviQI GTAC 2 cut(s) 300, 689
DdeI CTNAG 1 cut(s) 226
EaeI YGGCCR 2 cut(s) 244, 532
Eam1104I CTCTTC 2 cut(s) 227, 509
EarI CTCTTC 2 cut(s) 227, 509
Ecl136II GAGCTC 1 cut(s) 1160
Eco105I TACGTA 1 cut(s) 908
Eco130I CCWWGG 2 cut(s) 397, 865
Eco24I GRGCYC 1 cut(s) 1162
Eco53kI GAGCTC 1 cut(s) 1160
Eco91I GGTNACC 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 1160
EcoO65I GGTNACC 1 cut(s) 336
EcoRII CCWGG 3 cut(s) 130, 620, 921
EcoT14I CCWWGG 2 cut(s) 397, 865
EcoT38I GRGCYC 1 cut(s) 1162
ErhI CCWWGG 2 cut(s) 397, 865
FaeI CATG 5 cut(s) 336, 391, 401, 838, 1096
FaqI GGGAC 2 cut(s) 175, 965
FatI CATG 5 cut(s) 332, 387, 397, 834, 1092
FbaI TGATCA 3 cut(s) 792, 814, 976
FblI GTMKAC 1 cut(s) 904
Fnu4HI GCNGC 4 cut(s) 247, 454, 554, 994
FokI GGATG 4 cut(s) 48, 61, 478, 790
FriOI GRGCYC 1 cut(s) 1162
Fsp4HI GCNGC 4 cut(s) 247, 454, 554, 994
FspBI CTAG 1 cut(s) 365
GlaI GCGC 2 cut(s) 217, 285
GluI GCNGC 4 cut(s) 247, 454, 554, 994
GsuI CTGGAG 2 cut(s) 604, 1041
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 3 cut(s) 135, 246, 534
HapII CCGG 1 cut(s) 371
HhaI GCGC 2 cut(s) 218, 286
Hin1II CATG 5 cut(s) 336, 391, 401, 838, 1096
Hin6I GCGC 2 cut(s) 216, 284
HinP1I GCGC 2 cut(s) 216, 284
HinfI GANTC 6 cut(s) 95, 151, 668, 752, 1023, 1172
HpaII CCGG 1 cut(s) 371
HphI GGTGA 4 cut(s) 330, 668, 824, 854
Hpy166II GTNNAC 3 cut(s) 52, 627, 905
Hpy188III TCNNGA 3 cut(s) 388, 835, 1103
Hpy8I GTNNAC 3 cut(s) 52, 627, 905
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 4 cut(s) 330, 489, 631, 800
HpyCH4IV ACGT 2 cut(s) 907, 1177
HpyCH4V TGCA 2 cut(s) 52, 1131
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 255, 553
HpyF3I CTNAG 1 cut(s) 226
HpySE526I ACGT 2 cut(s) 907, 1177
Hsp92II CATG 5 cut(s) 336, 391, 401, 838, 1096
HspAI GCGC 2 cut(s) 216, 284
Ksp22I TGATCA 3 cut(s) 792, 814, 976
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 1 cut(s) 986
Lsp1109I GCAGC 3 cut(s) 440, 565, 1005
LweI GCATC 1 cut(s) 70
MaeI CTAG 1 cut(s) 365
MaeII ACGT 2 cut(s) 907, 1177
MaeIII GTNAC 3 cut(s) 336, 914, 1173
MboII GAAGA 7 cut(s) 95, 214, 470, 496, 636, 974, 1127
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 5 cut(s) 31, 229, 601, 636, 781
MhlI GDGCHC 3 cut(s) 54, 707, 1162
MlsI TGGCCA 1 cut(s) 534
MluNI TGGCCA 1 cut(s) 534
MlyI GAGTC 3 cut(s) 677, 1032, 1181
MmeI TCCRAC 1 cut(s) 989
Mox20I TGGCCA 1 cut(s) 534
MroXI GAANNNNTTC 1 cut(s) 1048
MscI TGGCCA 1 cut(s) 534
MseI TTAA 4 cut(s) 104, 758, 876, 1180
MslI CAYNNNNRTG 2 cut(s) 343, 346
Msp20I TGGCCA 1 cut(s) 534
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 3 cut(s) 132, 622, 923
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 3 cut(s) 132, 622, 923
MwoI GCNNNNNNNGC 3 cut(s) 58, 255, 553
NcoI CCATGG 1 cut(s) 397
NheI GCTAGC 1 cut(s) 364
NlaIII CATG 5 cut(s) 336, 391, 401, 838, 1096
NlaIV GGNNCC 4 cut(s) 33, 603, 783, 871
NmuCI GTSAC 2 cut(s) 336, 1173
NspI RCATGY 1 cut(s) 1096
OliI CACNNNNGTG 1 cut(s) 343
PagI TCATGA 2 cut(s) 387, 834
PciI ACATGT 1 cut(s) 1092
PciSI GCTCTTC 1 cut(s) 509
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 1048
PfeI GAWTC 3 cut(s) 95, 151, 752
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 4 cut(s) 248, 455, 555, 995
PleI GAGTC 3 cut(s) 676, 1031, 1180
PpsI GAGTC 3 cut(s) 676, 1031, 1180
Ppu21I YACGTR 1 cut(s) 908
PscI ACATGT 1 cut(s) 1092
PshAI GACNNNNGTC 1 cut(s) 1022
PshBI ATTAAT 1 cut(s) 104
PsiI TTATAA 2 cut(s) 435, 822
Psp124BI GAGCTC 1 cut(s) 1162
Psp6I CCWGG 3 cut(s) 130, 620, 921
PspEI GGTNACC 1 cut(s) 336
PspGI CCWGG 3 cut(s) 130, 620, 921
PspN4I GGNNCC 4 cut(s) 33, 603, 783, 871
PsuI RGATCY 5 cut(s) 31, 229, 601, 636, 781
RsaI GTAC 2 cut(s) 301, 690
RsaNI GTAC 2 cut(s) 300, 689
RseI CAYNNNNRTG 2 cut(s) 343, 346
SacI GAGCTC 1 cut(s) 1162
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 4 cut(s) 104, 758, 876, 1180
SatI GCNGC 4 cut(s) 247, 454, 554, 994
SchI GAGTC 3 cut(s) 677, 1032, 1181
ScrFI CCNGG 3 cut(s) 132, 622, 923
SduI GDGCHC 3 cut(s) 54, 707, 1162
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 343, 346
SnaBI TACGTA 1 cut(s) 908
SsiI CCGC 4 cut(s) 55, 247, 313, 894
SspI AATATT 1 cut(s) 118
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 1 cut(s) 1162
StyD4I CCNGG 3 cut(s) 130, 620, 921
StyI CCWWGG 2 cut(s) 397, 865
TaaI ACNGT 4 cut(s) 330, 489, 631, 800
TaiI ACGT 2 cut(s) 910, 1180
TaqI TCGA 1 cut(s) 67
TaqII GACCGA 2 cut(s) 509, 661
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 3 cut(s) 95, 151, 752
Tru1I TTAA 4 cut(s) 104, 758, 876, 1180
Tru9I TTAA 4 cut(s) 104, 758, 876, 1180
TscAI CASTG 4 cut(s) 54, 200, 1003, 1038
TseFI GTSAC 2 cut(s) 336, 1173
TseI GCWGC 3 cut(s) 453, 553, 993
Tsp45I GTSAC 2 cut(s) 336, 1173
TspDTI ATGAA 7 cut(s) 30, 63, 128, 823, 851, 864, 1128
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 4 cut(s) 54, 200, 1003, 1038
VneI GTGCAC 1 cut(s) 50
VspI ATTAAT 1 cut(s) 104
XapI RAATTY 4 cut(s) 697, 839, 1119, 1148
XceI RCATGY 1 cut(s) 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XmiI GTMKAC 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 1048
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.