Rorug01G0089400

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017777
Physical Location & Seq
Forward (+)
14561562 .. 14570469
8908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0089400.1

Sequence Viewer

Length: 3189 bp
ATGGATTCCGGTCGCTATGGTCTTCAGCAAGGATGGGATAATAACAGCGCTCCGGAGGGGTATGGTGCAGTTCATGAGCCAAACTTTCGGGTCGGTGGTTCATATGATGAGAGAAGGTTTATAGAGGAAAGGTACTCAAGGGACAATGTTTATCCAAGAAGCACATTCCACCGTGATGTTCTGGATAGGGATAACTACCCACCACCACCTCATGCTGTTGGTATATGGCCACATTCAAGAAGGAGAAGTTACGAAGAAGAATTTCCCATTGATAGGGAACCTAGGAGACATGAGAAACAGTACATGGATTCATATCATGAGATGGATAACCTCCGTGATCATGAGATTGATACATTTCCGGAATTTGATAAATTACGGGATGGCTATCACAGTGTTGACAGCTATCGGGATCCTGGGTTTGAGAGGACTTCAAGGCTTGGGGGACGTGATCGTGATGATTATGCATATGATGATTTTGACTATAGGTCCGGTATTACCCATAAGACCAGGGGGGACAGTCGTGAAAGGGATTATGACTATGGTCGGCATAGTTATGATTCTGATTATGACAGAGGTAGTAGGAGAGAGGGCAATTGGAGGCGACGTGAATCTCGTGACCGTGAGCGTGACAAGAAATGTTTAAGTAGGGAAAGAGAAATGAGTCCATATAGAAGGCATGAGCGTTCTAGATCTCGATCTCGATCTCGATCTCATGGGCATGATGATCATCCCAGATCAAGGTCACCTAGAAGTCGAAGCCATGGCCGAAGTCATCGAGAGGACAGCTATGATGATGGAAGGTATGAGAGAACTGATAAGCGAAGAGACAGGGATGAGAAACGCCAGAGAGAGCATTATTCTGTGGCCCCATCTGCAACTATTGTTGTGAAGGGTCTTTCACAGAAGACCACTGAGGAAGATCTATACCAGATCCTTGCTGAATGGGGACCTCTTCGTCATGTCCGTGTGATCAAAGAACGTAATTCCGGCATTTCCCGTGGATTTGCTTTTATTGATTTCCCTTCTGTGGGAGCAGCTCGTATTATGATGGACAAACTCGGGGACAATGGGCATGTGGTGGATGGCAGGAAACTATTTTTTGAGTACAGTAGTAAGCCAACTGGGGGTGCAGGTGGATCTTTTGGTCAGGACAGTGCTGGGAAATCTGGCCATGCTAACCATAAAAGCATCACTGTGCCATCTGATTGGATGTGTATCAGCTGTGGATATGTTAATTTTGCACGAAGAACATCCTGCTTTCAGTGTAATGAGGCACGAACTGAGGATGCTCCCGCAGCAGATATTTCTTCATCCAATCAAATGACAGGAAAGAAAGGATCAGAGGCTGGTCCTACTCATGTTTTGGTTGTTCGTGGACTGGATGAAAATGCTGATGAGGAAATGCTGCGCTATGAATTTTCCAAACATGCTCCTATCAAGGATCTACGTCTTGTCAGAGACAAATTCACTCATGTTTCACGGGGATTTGCGTTTGTACACTTCCATTCGGTGGAAGATGCTACGAAAGCTCTTGAAGCAACAAATGGTACACCACTTGAAAGGAATGGGCAGATTTTAAGAGTAGCATATGCAAAAAGCATTCTTGGTCCAGGATCAGGCATGTCAGGATCTTCACAGTCAAGCAGCTTGGCAGCTGCTGCAATTGAGGCAGCAACATTTGCTCAACAGTATGATGCTGTTGGATGGGCACCAAAGGAATATAATCCAGACGACAAACAATTCACGGGAGGGAAGGAGCAAAGTGGCGGGGATTTAAAAGCAGAAAAAGATGGTTTGGCTCCACAAACTGGTTTTGTATGGGATGAGGCATCCGGTTACTACTATGATGCTTCTTCCGGGTTCTACTATGATGGAAATACAGGTCTCTATTATGATGGTAACAATGGGATTTGGTATTCTTATGATCACCAAACTCTGCAGTACATCCCTTGCACTGATCAGAATGGCATCAAAGAATCTGCTAACCAATCTCAGCTTTCGAAGGCATCAGACGGTTCAAGTACCAAAAAAGTAGTCATCTCTGCACCAGCTACAACCTCAGCTGCATCATTACCAGATGCAGTCCAGGCTGCTGCAACAGCAGCGATAGCTGCAGAGAGAAAAGAAAAGGAAAAGTCCAAAGAGATCAAGTTGGCATCAAAGAGCAGTATTTTGGCTAACAAGAAGAAAATGAGTAATGTATTGACTATGTGGAAGCAGAGGAGTCATGAAGGGCAAGTAACCCGTGTAGCTCTAGATGATAACCAGCCAAGTGCACCTGCTAATGATAGACTGGTTTCTTCTGTTCCATCTACAAAGACAAAGTCTAAAATTGATGTACCAACCAAAAATGAGAATACTATATCTAGCTCAGGGGTTACTGCCAATGTTTCTACTGCAGATACTCTTGGTTTAGAGTCTTCAGTCATGCCAAGGCCTGTGGGCAACAGTATAGGAGGGACACTGATGGGAGTGATAAGGGGATCTGGAAGAACTGTAGTAAAATCAGATACATCCTTTTCAGCATCTGGTGGAGTTTCCGCACCTTCAACTTCTGCTGCATGCATAGCAGGTTCTTCTGTATCAACAGATTCGGATATCTCCACTGTTTTGACACCCTTCAGAACTGATGCATCGGCACTAGGTTCTTATACACCACCTGTGGCTGCTGGGAGTGGCAAGAGAAGATTCTCTGAAATGCCAGCTTCTGCTCATAAAGAACCTCAAACTGGATACAGGGATCGTGCGGCTGAAAGGAGGAGCTTGTATGGCTCGTCATCTTCTTTTGGAGATGATTTGCCTGAACATGGTTTTGGGGAGTCAAATCGAGACTCGGCATTGAGGAAGGGTTCTTTTGATTCAATGCCTTTCCCCCCTGGTGTTGGTGGAGGACGTGGGACCGGAGATGCAACCATTGACAGTTACGAGGTTATTACAGCCGACAAAGCAATTGATGAGAGCAATGTGGGCAACAAGATGCTTCGCAGCATGGGCTGGCACGAAGGCTTGGGTTTAGGAAGAGATGGGAGTGGAATGGTGGAACCGGTGCAGGCTCAGAGTGTGGAAAGGAGAGCCGGACTTGGGAGTCAGCAGAAGAAGTTAGATCCGGCTCTTGAGGCTCAGGCTGGAGATAGTTATAAAACTCTCATTCACAAGAAGGCACTGGCTAGGTTCCGGGAGATGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

1062

Amino Acids

117.43

Weight (kDa)

7.95

Isoelectric Point (pI)

56.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 294 - 364 1.7e-11 RNA recognition motif
RRM_1 PF00076 455 - 520 2.7e-17 RNA recognition motif
OCRE PF17780 602 - 650 1.8e-17 OCRE domain
G-patch PF01585 987 - 1028 3.7e-14 G-patch domain
G-patch_2 PF12656 987 - 1032 1.4e-06 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017777)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0330581
rosa_multiflora Rmu_sc0000167.1_g000006 Rmu_sc0000815.1_g000001
rosa_roxburghii Rroxscaffold_4G00319740
rosa_rugosa Rorug01G0089400 Rorug01G0089500 Rorug01G0089600
rosa_samantha Rh1AG107700 Rh1CG103500 Rh1DG113100
rosa_wichuraiana Rw0G004380 Rw1G008920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3138
AarI CACCTGC 2 cut(s) 1121, 2287
AasI GACNNNNNNGTC 2 cut(s) 954, 3084
Acc36I ACCTGC 3 cut(s) 1121, 2287, 2561
AccB1I GGYRCC 1 cut(s) 1708
AccB7I CCANNNNNTGG 2 cut(s) 1510, 1808
AccIII TCCGGA 2 cut(s) 52, 358
AciI CCGC 4 cut(s) 1293, 1767, 2541, 2747
AcoI YGGCCR 3 cut(s) 227, 763, 1168
AcsI RAATTY 4 cut(s) 260, 362, 1415, 1463
AcuI CTGAAG 3 cut(s) 8, 2406, 2605
AfaI GTAC 8 cut(s) 134, 302, 1106, 1497, 1549, 1943, 2023, 2340
AfeI AGCGCT 1 cut(s) 49
AgeI ACCGGT 1 cut(s) 3043
AgsI TTSAA 7 cut(s) 237, 432, 1535, 1559, 2019, 2550, 2862
AhdI GACNNNNNGTC 1 cut(s) 484
AjiI CACGTC 3 cut(s) 446, 605, 2894
AjnI CCWGG 5 cut(s) 412, 506, 1609, 2085, 2875
AjuI GAANNNNNNNTTGG 2 cut(s) 2796, 2828
Alw21I GWGCWC 1 cut(s) 2278
Alw26I GTCTC 5 cut(s) 280, 819, 1452, 1889, 2823
Alw44I GTGCAC 1 cut(s) 2274
AlwNI CAGNNNCTG 4 cut(s) 1346, 1658, 2528, 2708
Ama87I CYCGRG 1 cut(s) 1058
Aor13HI TCCGGA 2 cut(s) 52, 358
Aor51HI AGCGCT 1 cut(s) 49
AoxI GGCC 5 cut(s) 227, 763, 864, 1168, 2435
ApaLI GTGCAC 1 cut(s) 2274
ApoI RAATTY 4 cut(s) 260, 362, 1415, 1463
ArsI GACNNNNNNTTYG 4 cut(s) 2019, 2051, 2309, 2341
AsiGI ACCGGT 1 cut(s) 3043
Asp700I GAANNNNTTC 1 cut(s) 261
AspA2I CCTAGG 1 cut(s) 281
AspLEI GCGC 2 cut(s) 50, 1410
AspS9I GGNCC 6 cut(s) 486, 865, 947, 1349, 1607, 2898
AsuC2I CCSGG 2 cut(s) 1858, 3176
AsuHPI GGTGA 2 cut(s) 735, 1919
AsuII TTCGAA 1 cut(s) 2000
AvaI CYCGRG 1 cut(s) 1058
AvaII GGWCC 5 cut(s) 486, 947, 1349, 1607, 2898
AvrII CCTAGG 1 cut(s) 281
BaeGI GKGCMC 2 cut(s) 1711, 2278
BalI TGGCCA 2 cut(s) 229, 1170
BamHI GGATCC 1 cut(s) 409
BanI GGYRCC 1 cut(s) 1708
BauI CACGAG 1 cut(s) 612
BbsI GAAGAC 3 cut(s) 14, 911, 2412
Bbv12I GWGCWC 1 cut(s) 2278
BbvCI CCTCAGC 1 cut(s) 2059
BciT130I CCWGG 5 cut(s) 414, 508, 1611, 2087, 2877
BciVI GTATCC 1 cut(s) 2726
BclI TGATCA 5 cut(s) 337, 724, 969, 1924, 1957
BcnI CCSGG 2 cut(s) 1858, 3176
BcoDI GTCTC 5 cut(s) 280, 819, 1452, 1889, 2823
BfaI CTAG 7 cut(s) 282, 687, 747, 2255, 2367, 2642, 3168
BfmI CTRYAG 5 cut(s) 481, 1937, 2112, 2397, 2496
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 3 cut(s) 1121, 2287, 2561
BfuI GTATCC 1 cut(s) 2726
BglII AGATCT 2 cut(s) 689, 919
BlnI CCTAGG 1 cut(s) 281
Bme1390I CCNGG 7 cut(s) 414, 508, 1611, 1858, 2087, 2877, 3176
Bme18I GGWCC 5 cut(s) 486, 947, 1349, 1607, 2898
BmeRI GACNNNNNGTC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 1058
BmgBI CACGTC 3 cut(s) 446, 605, 2894
BmgT120I GGNCC 6 cut(s) 486, 865, 947, 1349, 1607, 2898
BmiI GGNNCC 9 cut(s) 279, 411, 867, 948, 1710, 1800, 2899, 3042, 3173
BmrFI CCNGG 7 cut(s) 414, 508, 1611, 1858, 2087, 2877, 3176
BmrI ACTGGG 1 cut(s) 1131
BmuI ACTGGG 1 cut(s) 1131
BoxI GACNNNNGTC 1 cut(s) 540
BpiI GAAGAC 3 cut(s) 14, 911, 2412
BpmI CTGGAG 1 cut(s) 3147
Bpu10I CCTNAGC 3 cut(s) 2059, 2371, 3120
Bpu14I TTCGAA 1 cut(s) 2000
BpuEI CTTGAG 2 cut(s) 121, 3134
BpuMI CCSGG 2 cut(s) 1858, 3176
BsaBI GATNNNNATC 7 cut(s) 312, 384, 694, 700, 706, 726, 1214
BsaI GGTCTC 1 cut(s) 1889
BsaJI CCNNGG 7 cut(s) 281, 413, 507, 760, 997, 2432, 2875
BsaWI WCCGGW 7 cut(s) 8, 52, 358, 488, 1832, 2900, 3043
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bse118I RCCGGY 1 cut(s) 3043
Bse1I ACTGG 6 cut(s) 1126, 1383, 1813, 2298, 2734, 3168
Bse3DI GCAATG 1 cut(s) 2968
Bse8I GATNNNNATC 7 cut(s) 312, 384, 694, 700, 706, 726, 1214
BseAI TCCGGA 2 cut(s) 52, 358
BseBI CCWGG 5 cut(s) 414, 508, 1611, 2087, 2877
BseDI CCNNGG 7 cut(s) 281, 413, 507, 760, 997, 2432, 2875
BseJI GATNNNNATC 7 cut(s) 312, 384, 694, 700, 706, 726, 1214
BseMI GCAATG 1 cut(s) 2968
BseMII CTCAG 7 cut(s) 903, 1272, 2006, 2073, 2385, 3068, 3134
BseNI ACTGG 6 cut(s) 1126, 1383, 1813, 2298, 2734, 3168
BseRI GAGGAG 2 cut(s) 2236, 2773
BseSI GKGCMC 2 cut(s) 1711, 2278
BseYI CCCAGC 2 cut(s) 1157, 2669
BsgI GTGCAG 4 cut(s) 87, 1149, 2028, 3068
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 5 cut(s) 229, 765, 866, 1170, 2437
BshNI GGYRCC 1 cut(s) 1708
BshTI ACCGGT 1 cut(s) 3043
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 2278
BsiHKCI CYCGRG 1 cut(s) 1058
BslFI GGGAC 7 cut(s) 155, 456, 527, 960, 1076, 2473, 2911
BsmAI GTCTC 5 cut(s) 280, 819, 1452, 1889, 2823
BsmFI GGGAC 7 cut(s) 155, 456, 527, 960, 1076, 2473, 2911
BsmI GAATGC 1 cut(s) 1599
BsnI GGCC 5 cut(s) 229, 765, 866, 1170, 2437
Bso31I GGTCTC 1 cut(s) 1889
BsoBI CYCGRG 1 cut(s) 1058
Bsp119I TTCGAA 1 cut(s) 2000
Bsp1286I GDGCHC 2 cut(s) 1711, 2278
Bsp13I TCCGGA 2 cut(s) 52, 358
Bsp1407I TGTACA 1 cut(s) 1495
Bsp19I CCATGG 1 cut(s) 760
BspACI CCGC 4 cut(s) 1293, 1767, 2541, 2747
BspANI GGCC 5 cut(s) 229, 765, 866, 1170, 2437
BspCNI CTCAG 7 cut(s) 904, 1273, 2005, 2072, 2384, 3067, 3133
BspEI TCCGGA 2 cut(s) 52, 358
BspHI TCATGA 4 cut(s) 73, 316, 340, 2227
BspLI GGNNCC 9 cut(s) 279, 411, 867, 948, 1710, 1800, 2899, 3042, 3173
BspMAI CTGCAG 3 cut(s) 1941, 2116, 2401
BspMI ACCTGC 3 cut(s) 1121, 2287, 2561
BspT104I TTCGAA 1 cut(s) 2000
BspT107I GGYRCC 1 cut(s) 1708
BspTNI GGTCTC 1 cut(s) 1889
BsrDI GCAATG 1 cut(s) 2968
BsrFI RCCGGY 1 cut(s) 3043
BsrGI TGTACA 1 cut(s) 1495
BsrI ACTGG 6 cut(s) 1126, 1383, 1813, 2298, 2734, 3168
BssAI RCCGGY 1 cut(s) 3043
BssECI CCNNGG 7 cut(s) 281, 413, 507, 760, 997, 2432, 2875
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 3 cut(s) 281, 760, 2432
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 5 cut(s) 414, 508, 1611, 2087, 2877
Bst6I CTCTTC 3 cut(s) 817, 957, 3013
BstAPI GCANNNNNTGC 2 cut(s) 1658, 1679
BstAUI TGTACA 1 cut(s) 1495
BstBI TTCGAA 1 cut(s) 2000
BstC8I GCNNGC 4 cut(s) 2563, 2703, 2996, 3051
BstDEI CTNAG 7 cut(s) 912, 1281, 1992, 2059, 2371, 3054, 3120
BstDSI CCRYGG 2 cut(s) 760, 997
BstEII GGTNACC 1 cut(s) 741
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 1410
BstMAI GTCTC 5 cut(s) 280, 819, 1452, 1889, 2823
BstMCI CGRYCG 1 cut(s) 13
BstNI CCWGG 5 cut(s) 414, 508, 1611, 2087, 2877
BstNSI RCATGY 4 cut(s) 1076, 1430, 1624, 2565
BstPAI GACNNNNGTC 1 cut(s) 540
BstPI GGTNACC 1 cut(s) 741
BstSCI CCNGG 7 cut(s) 412, 506, 1609, 1856, 2085, 2875, 3174
BstSFI CTRYAG 5 cut(s) 481, 1937, 2112, 2397, 2496
BstSLI GKGCMC 2 cut(s) 1711, 2278
BstV2I GAAGAC 3 cut(s) 14, 911, 2412
BstX2I RGATCY 9 cut(s) 409, 689, 919, 930, 1136, 1441, 1628, 2483, 3103
BstXI CCANNNNNNTGG 1 cut(s) 1206
BstYI RGATCY 9 cut(s) 409, 689, 919, 930, 1136, 1441, 1628, 2483, 3103
BsuI GTATCC 1 cut(s) 2726
BsuRI GGCC 5 cut(s) 229, 765, 866, 1170, 2437
BtgI CCRYGG 2 cut(s) 760, 997
BtrI CACGTC 3 cut(s) 446, 605, 2894
BtsIMutI CAGTG 9 cut(s) 397, 909, 1159, 1191, 1268, 1953, 2462, 2604, 3161
BveI ACCTGC 3 cut(s) 1121, 2287, 2561
Cac8I GCNNGC 4 cut(s) 2563, 2703, 2996, 3051
CaiI CAGNNNCTG 4 cut(s) 1346, 1658, 2528, 2708
CciI TCATGA 4 cut(s) 73, 316, 340, 2227
CfoI GCGC 2 cut(s) 50, 1410
Cfr10I RCCGGY 1 cut(s) 3043
Cfr13I GGNCC 6 cut(s) 486, 865, 947, 1349, 1607, 2898
Csp6I GTAC 8 cut(s) 133, 301, 1105, 1496, 1548, 1942, 2022, 2339
CspAI ACCGGT 1 cut(s) 3043
CspCI CAANNNNNGTGG 2 cut(s) 2421, 2456
CviQI GTAC 8 cut(s) 133, 301, 1105, 1496, 1548, 1942, 2022, 2339
DdeI CTNAG 7 cut(s) 912, 1281, 1992, 2059, 2371, 3054, 3120
DraI TTTAAA 1 cut(s) 1776
DrdI GACNNNNNNGTC 2 cut(s) 954, 3084
DriI GACNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 2 cut(s) 954, 3084
EaeI YGGCCR 3 cut(s) 227, 763, 1168
Eam1104I CTCTTC 3 cut(s) 817, 957, 3013
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 3 cut(s) 817, 957, 3013
Eco130I CCWWGG 3 cut(s) 281, 760, 2432
Eco147I AGGCCT 1 cut(s) 2437
Eco31I GGTCTC 1 cut(s) 1889
Eco32I GATATC 1 cut(s) 2599
Eco47I GGWCC 5 cut(s) 486, 947, 1349, 1607, 2898
Eco47III AGCGCT 1 cut(s) 49
Eco57I CTGAAG 3 cut(s) 8, 2406, 2605
Eco88I CYCGRG 1 cut(s) 1058
Eco91I GGTNACC 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 947
EcoO65I GGTNACC 1 cut(s) 741
EcoRII CCWGG 5 cut(s) 412, 506, 1609, 2085, 2875
EcoRV GATATC 1 cut(s) 2599
EcoT14I CCWWGG 3 cut(s) 281, 760, 2432
EcoT22I ATGCAT 3 cut(s) 466, 2567, 2635
ErhI CCWWGG 3 cut(s) 281, 760, 2432
FaqI GGGAC 7 cut(s) 155, 456, 527, 960, 1076, 2473, 2911
FauI CCCGC 2 cut(s) 1300, 1760
FauNDI CATATG 3 cut(s) 103, 466, 1588
FbaI TGATCA 5 cut(s) 337, 724, 969, 1924, 1957
FspBI CTAG 7 cut(s) 282, 687, 747, 2255, 2367, 2642, 3168
GlaI GCGC 2 cut(s) 49, 1409
GsaI CCCAGC 2 cut(s) 1161, 2673
GsuI CTGGAG 1 cut(s) 3147
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 5 cut(s) 229, 765, 866, 1170, 2437
HhaI GCGC 2 cut(s) 50, 1410
Hin6I GCGC 2 cut(s) 48, 1408
HinP1I GCGC 2 cut(s) 48, 1408
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HphI GGTGA 2 cut(s) 735, 1919
Hpy166II GTNNAC 5 cut(s) 397, 1376, 1498, 1550, 2276
Hpy8I GTNNAC 5 cut(s) 397, 1376, 1498, 1550, 2276
Hpy99I CGWCG 1 cut(s) 606
HpyCH4IV ACGT 5 cut(s) 445, 604, 979, 1447, 2893
HpyF3I CTNAG 7 cut(s) 912, 1281, 1992, 2059, 2371, 3054, 3120
HpySE526I ACGT 5 cut(s) 445, 604, 979, 1447, 2893
HspAI GCGC 2 cut(s) 48, 1408
Kpn2I TCCGGA 2 cut(s) 52, 358
Ksp22I TGATCA 5 cut(s) 337, 724, 969, 1924, 1957
LmnI GCTCC 7 cut(s) 55, 1031, 1294, 1435, 1756, 1804, 2760
MaeI CTAG 7 cut(s) 282, 687, 747, 2255, 2367, 2642, 3168
MaeII ACGT 5 cut(s) 445, 604, 979, 1447, 2893
MaeIII GTNAC 9 cut(s) 248, 614, 626, 741, 1835, 1898, 2239, 2377, 2921
MfeI CAATTG 3 cut(s) 592, 1662, 2949
MflI RGATCY 9 cut(s) 409, 689, 919, 930, 1136, 1441, 1628, 2483, 3103
MhlI GDGCHC 2 cut(s) 1711, 2278
MlsI TGGCCA 2 cut(s) 229, 1170
MluNI TGGCCA 2 cut(s) 229, 1170
MlyI GAGTC 6 cut(s) 670, 2233, 2426, 2825, 2828, 3094
MmeI TCCRAC 1 cut(s) 1681
Mox20I TGGCCA 2 cut(s) 229, 1170
Mph1103I ATGCAT 3 cut(s) 466, 2567, 2635
MroI TCCGGA 2 cut(s) 52, 358
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 2 cut(s) 229, 1170
MseI TTAA 4 cut(s) 641, 1233, 1577, 1775
MslI CAYNNNNRTG 5 cut(s) 174, 552, 717, 963, 1193
Msp20I TGGCCA 2 cut(s) 229, 1170
MspA1I CMGCKG 3 cut(s) 1221, 1655, 2063
MspR9I CCNGG 7 cut(s) 414, 508, 1611, 1858, 2087, 2877, 3176
MunI CAATTG 3 cut(s) 592, 1662, 2949
Mva1269I GAATGC 1 cut(s) 1599
MvaI CCWGG 5 cut(s) 414, 508, 1611, 2087, 2877
NciI CCSGG 2 cut(s) 1858, 3176
NcoI CCATGG 1 cut(s) 760
NdeI CATATG 3 cut(s) 103, 466, 1588
NlaIV GGNNCC 9 cut(s) 279, 411, 867, 948, 1710, 1800, 2899, 3042, 3173
NmeAIII GCCGAG 1 cut(s) 2813
NmuCI GTSAC 3 cut(s) 614, 626, 741
NsiI ATGCAT 3 cut(s) 466, 2567, 2635
NspI RCATGY 4 cut(s) 1076, 1430, 1624, 2565
NspV TTCGAA 1 cut(s) 2000
PaeI GCATGC 1 cut(s) 2565
PagI TCATGA 4 cut(s) 73, 316, 340, 2227
PaqCI CACCTGC 2 cut(s) 1121, 2287
PceI AGGCCT 1 cut(s) 2437
PcsI WCGNNNNNNNCGW 2 cut(s) 610, 961
PctI GAATGC 1 cut(s) 1599
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 8 cut(s) 5, 308, 557, 608, 1976, 2591, 2688, 2858
PflFI GACNNNGTC 1 cut(s) 2323
PflMI CCANNNNNTGG 2 cut(s) 1510, 1808
PfoI TCCNGGA 2 cut(s) 1609, 3174
PinAI ACCGGT 1 cut(s) 3043
PleI GAGTC 6 cut(s) 669, 2232, 2425, 2825, 2827, 3093
PpsI GAGTC 6 cut(s) 669, 2232, 2425, 2825, 2827, 3093
PpuMI RGGWCCY 1 cut(s) 947
PshAI GACNNNNGTC 1 cut(s) 540
PsiI TTATAA 1 cut(s) 3138
Psp5II RGGWCCY 1 cut(s) 947
Psp6I CCWGG 5 cut(s) 412, 506, 1609, 2085, 2875
PspEI GGTNACC 1 cut(s) 741
PspFI CCCAGC 2 cut(s) 1157, 2669
PspGI CCWGG 5 cut(s) 412, 506, 1609, 2085, 2875
PspN4I GGNNCC 9 cut(s) 279, 411, 867, 948, 1710, 1800, 2899, 3042, 3173
PspPI GGNCC 6 cut(s) 486, 865, 947, 1349, 1607, 2898
PspPPI RGGWCCY 1 cut(s) 947
PstI CTGCAG 3 cut(s) 1941, 2116, 2401
PstNI CAGNNNCTG 4 cut(s) 1346, 1658, 2528, 2708
PsuI RGATCY 9 cut(s) 409, 689, 919, 930, 1136, 1441, 1628, 2483, 3103
PsyI GACNNNGTC 1 cut(s) 2323
PvuII CAGCTG 3 cut(s) 1221, 1655, 2063
RsaI GTAC 8 cut(s) 134, 302, 1106, 1497, 1549, 1943, 2023, 2340
RsaNI GTAC 8 cut(s) 133, 301, 1105, 1496, 1548, 1942, 2022, 2339
RseI CAYNNNNRTG 5 cut(s) 174, 552, 717, 963, 1193
SaqAI TTAA 4 cut(s) 641, 1233, 1577, 1775
Sau96I GGNCC 6 cut(s) 486, 865, 947, 1349, 1607, 2898
SchI GAGTC 6 cut(s) 670, 2233, 2426, 2825, 2828, 3094
ScrFI CCNGG 7 cut(s) 414, 508, 1611, 1858, 2087, 2877, 3176
SduI GDGCHC 2 cut(s) 1711, 2278
SfcI CTRYAG 5 cut(s) 481, 1937, 2112, 2397, 2496
SfuI TTCGAA 1 cut(s) 2000
SinI GGWCC 5 cut(s) 486, 947, 1349, 1607, 2898
SmiMI CAYNNNNRTG 5 cut(s) 174, 552, 717, 963, 1193
SmlI CTYRAG 2 cut(s) 136, 3113
SmoI CTYRAG 2 cut(s) 136, 3113
SphI GCATGC 1 cut(s) 2565
SseBI AGGCCT 1 cut(s) 2437
SsiI CCGC 4 cut(s) 1293, 1767, 2541, 2747
SspMI CTAG 7 cut(s) 282, 687, 747, 2255, 2367, 2642, 3168
StuI AGGCCT 1 cut(s) 2437
StyD4I CCNGG 7 cut(s) 412, 506, 1609, 1856, 2085, 2875, 3174
StyI CCWWGG 3 cut(s) 281, 760, 2432
TaiI ACGT 5 cut(s) 448, 607, 982, 1450, 2896
TaqI TCGA 7 cut(s) 694, 700, 706, 754, 775, 2000, 2827
TatI WGTACW 4 cut(s) 300, 1104, 1495, 1941
TauI GCSGC 1 cut(s) 2750
TfiI GAWTC 8 cut(s) 5, 308, 557, 608, 1976, 2591, 2688, 2858
Tru1I TTAA 4 cut(s) 641, 1233, 1577, 1775
Tru9I TTAA 4 cut(s) 641, 1233, 1577, 1775
TscAI CASTG 9 cut(s) 397, 916, 1159, 1198, 1268, 1960, 2469, 2611, 3168
TseFI GTSAC 3 cut(s) 614, 626, 741
Tsp45I GTSAC 3 cut(s) 614, 626, 741
TspDTI ATGAA 7 cut(s) 62, 90, 300, 1299, 1398, 1428, 2244
TspGWI ACGGA 2 cut(s) 323, 953
TspRI CASTG 9 cut(s) 397, 916, 1159, 1198, 1268, 1960, 2469, 2611, 3168
Tth111I GACNNNGTC 1 cut(s) 2323
Van91I CCANNNNNTGG 2 cut(s) 1510, 1808
VneI GTGCAC 1 cut(s) 2274
VpaK11BI GGWCC 5 cut(s) 486, 947, 1349, 1607, 2898
XapI RAATTY 4 cut(s) 260, 362, 1415, 1463
XbaI TCTAGA 2 cut(s) 686, 2254
XceI RCATGY 4 cut(s) 1076, 1430, 1624, 2565
XmaJI CCTAGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 7 cut(s) 282, 687, 747, 2255, 2367, 2642, 3168
Zsp2I ATGCAT 3 cut(s) 466, 2567, 2635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.