Rorug01G0089600

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017777
Physical Location & Seq
Forward (+)
14586903 .. 14587779
877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0089600.1

Sequence Viewer

Length: 840 bp
ATGGGTATAACTGGAAGGTCCTTTACTCAGTCTGCATGGACTTTGATGGTAGTCATTCTTGCTTGTAGCTCTAAGTCGACAGATAGTCTTAAGTACTGTACCCCGTCTTCTTGCGGCAATATCCATAACATAAGCTACCCTTTCCGACTAAAAAATGATCCAAAGCACTGCGGCCACGTAGCCTATACTCTCAATTGTGAGAACAACAGTACTCTCTTAGACTTTTCTTCTGGGAGATACCACGTAAAGGCAATCAACTATCATAACCAAACAATCCGAGTCGTGGATCCTGGCTTTCAGAAGAACAATTGCTCCTCCGTTCCTCTTTATTCCTCACACAATTTTTATGATTGGTTAACGTATGAATTTGATAATATATCCTCTAATTTTTGGGAACCTCTTTCAATCTCTATATCCTTTCTCAAGTGTTCACATCCAGTGAATTCTTCTCTGTATGTGAACACTGCTGCATGCAATGATACTAGTACTAGCAGTACTGGTACTTCTTCTTCTTCTTCTTTGTCCCATCCCAAAAGGTATGGTTATATTTTCTATACATTTCAGTATCCTCCTGCCATGATGTCAGGGGACGTGGAGGAGGGTTGCACCTTAGAGTGGATGAGTTTTGCACCTTCCCGACTTTTTGTAAACACAACCGAAGACCCATCTTTCACATACATAGACATACAAAATGCACTGGTGGATGGCTTTGAGCTTGGATTTGGGAATGATTATATATATGATGATGGTCCATGCGAGGGCTGGTATGATCAATATAATTCTGGCAAATGTTTTCCTCGCACAGTTGCAGCTGCGTTCCGTCATTCTCAAGTACATTAA

Protein Analysis

279

Amino Acids

31.35

Weight (kDa)

5.74

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 96 3.4e-20 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017777)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0330581
rosa_multiflora Rmu_sc0000167.1_g000006 Rmu_sc0000815.1_g000001
rosa_roxburghii Rroxscaffold_4G00319740
rosa_rugosa Rorug01G0089400 Rorug01G0089500 Rorug01G0089600
rosa_samantha Rh1AG107700 Rh1CG103500 Rh1DG113100
rosa_wichuraiana Rw0G004380 Rw1G008920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 77
AciI CCGC 2 cut(s) 114, 171
AclWI GGATC 3 cut(s) 152, 281, 294
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 2 cut(s) 365, 442
AfaI GTAC 7 cut(s) 95, 100, 211, 487, 496, 502, 834
AfiI CCNNNNNNNGG 4 cut(s) 247, 283, 615, 758
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 1 cut(s) 405
AhdI GACNNNNNGTC 1 cut(s) 84
AhlI ACTAGT 1 cut(s) 482
AjiI CACGTC 1 cut(s) 592
AjnI CCWGG 1 cut(s) 289
AloI GAACNNNNNNTCC 2 cut(s) 296, 328
AluBI AGCT 4 cut(s) 69, 135, 715, 812
AluI AGCT 4 cut(s) 69, 135, 715, 812
AlwI GGATC 3 cut(s) 152, 281, 294
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 3 cut(s) 467, 809, 812
ApoI RAATTY 2 cut(s) 365, 442
AspS9I GGNCC 2 cut(s) 18, 749
AvaII GGWCC 2 cut(s) 18, 749
BamHI GGATCC 1 cut(s) 286
BarI GAAGNNNNNNTAC 8 cut(s) 7, 39, 91, 123, 487, 493, 519, 525
BbsI GAAGAC 2 cut(s) 99, 666
BbvI GCAGC 3 cut(s) 454, 799, 821
BccI CCATC 5 cut(s) 40, 534, 673, 698, 740
BciT130I CCWGG 1 cut(s) 291
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 769
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 2 cut(s) 483, 489
BfrI CTTAAG 1 cut(s) 89
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 5 cut(s) 115, 172, 468, 810, 813
BlsI GCNGC 5 cut(s) 116, 173, 469, 811, 814
BmcAI AGTACT 4 cut(s) 95, 211, 487, 496
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 18, 749
BmeRI GACNNNNNGTC 1 cut(s) 84
BmgBI CACGTC 1 cut(s) 592
BmgT120I GGNCC 2 cut(s) 18, 749
BmiI GGNNCC 2 cut(s) 288, 396
BmrFI CCNGG 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 99, 666
BpuEI CTTGAG 2 cut(s) 407, 813
BsaAI YACGTR 2 cut(s) 178, 244
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 283, 615, 758
Bse1I ACTGG 4 cut(s) 16, 437, 502, 702
Bse3DI GCAATG 1 cut(s) 481
BseBI CCWGG 1 cut(s) 291
BseGI GGATG 4 cut(s) 433, 526, 624, 709
BseLI CCNNNNNNNGG 4 cut(s) 247, 283, 615, 758
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 4 cut(s) 16, 437, 502, 702
BseRI GAGGAG 2 cut(s) 304, 611
BseXI GCAGC 3 cut(s) 454, 799, 821
BshFI GGCC 1 cut(s) 174
BslFI GGGAC 2 cut(s) 508, 602
BslI CCNNNNNNNGG 4 cut(s) 247, 283, 615, 758
BsmFI GGGAC 2 cut(s) 508, 602
BsnI GGCC 1 cut(s) 174
Bsp143I GATC 3 cut(s) 157, 286, 769
BspACI CCGC 2 cut(s) 114, 171
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 288, 396
BspPI GGATC 3 cut(s) 152, 281, 294
BspTI CTTAAG 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 481
BsrI ACTGG 4 cut(s) 16, 437, 502, 702
BssMI GATC 3 cut(s) 157, 286, 769
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 98, 209, 805
BstAFI CTTAAG 1 cut(s) 89
BstBAI YACGTR 2 cut(s) 178, 244
BstC8I GCNNGC 1 cut(s) 472
BstDEI CTNAG 4 cut(s) 27, 72, 217, 610
BstF5I GGATG 4 cut(s) 433, 526, 624, 709
BstKTI GATC 3 cut(s) 160, 289, 772
BstMBI GATC 3 cut(s) 157, 286, 769
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 474
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 454, 799, 821
BstV2I GAAGAC 2 cut(s) 99, 666
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 1 cut(s) 174
BtrI CACGTC 1 cut(s) 592
BtsCI GGATG 4 cut(s) 433, 526, 624, 709
BtsI GCAGTG 2 cut(s) 166, 462
BtsIMutI CAGTG 4 cut(s) 166, 444, 462, 695
Cac8I GCNNGC 1 cut(s) 472
Cfr13I GGNCC 2 cut(s) 18, 749
Csp6I GTAC 7 cut(s) 94, 99, 210, 486, 495, 501, 833
CviAII CATG 4 cut(s) 36, 471, 577, 753
CviJI RGCY 9 cut(s) 69, 135, 174, 182, 294, 708, 715, 762, 812
CviKI_1 RGCY 9 cut(s) 69, 135, 174, 182, 294, 708, 715, 762, 812
CviQI GTAC 7 cut(s) 94, 99, 210, 486, 495, 501, 833
DdeI CTNAG 4 cut(s) 27, 72, 217, 610
DpnI GATC 3 cut(s) 159, 288, 771
DpnII GATC 3 cut(s) 157, 286, 769
DriI GACNNNNNGTC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 172
Eam1105I GACNNNNNGTC 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 18, 749
EcoO109I RGGNCCY 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 4 cut(s) 39, 474, 580, 756
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FaqI GGGAC 2 cut(s) 508, 602
FatI CATG 4 cut(s) 35, 470, 576, 752
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 5 cut(s) 115, 172, 468, 810, 813
FokI GGATG 4 cut(s) 420, 513, 631, 716
Fsp4HI GCNGC 5 cut(s) 115, 172, 468, 810, 813
FspBI CTAG 2 cut(s) 483, 489
GluI GCNGC 5 cut(s) 115, 172, 468, 810, 813
HaeIII GGCC 1 cut(s) 174
Hin1II CATG 4 cut(s) 39, 474, 580, 756
HincII GTYRAC 2 cut(s) 78, 357
HindII GTYRAC 2 cut(s) 78, 357
HinfI GANTC 1 cut(s) 279
HpaI GTTAAC 1 cut(s) 357
Hpy166II GTNNAC 5 cut(s) 78, 357, 431, 460, 649
Hpy188I TCNGA 3 cut(s) 146, 278, 300
Hpy188III TCNNGA 1 cut(s) 636
Hpy8I GTNNAC 5 cut(s) 78, 357, 431, 460, 649
HpyAV CCTTC 2 cut(s) 9, 642
HpyCH4III ACNGT 3 cut(s) 98, 209, 805
HpyCH4IV ACGT 4 cut(s) 177, 243, 359, 591
HpyCH4V TGCA 7 cut(s) 35, 470, 474, 606, 629, 695, 809
HpyF3I CTNAG 4 cut(s) 27, 72, 217, 610
HpySE526I ACGT 4 cut(s) 177, 243, 359, 591
Hsp92II CATG 4 cut(s) 39, 474, 580, 756
Ksp22I TGATCA 1 cut(s) 769
KspAI GTTAAC 1 cut(s) 357
Kzo9I GATC 3 cut(s) 157, 286, 769
LmnI GCTCC 1 cut(s) 317
Lsp1109I GCAGC 3 cut(s) 454, 799, 821
MaeI CTAG 2 cut(s) 483, 489
MaeII ACGT 4 cut(s) 177, 243, 359, 591
MalI GATC 3 cut(s) 159, 288, 771
MboI GATC 3 cut(s) 157, 286, 769
MboII GAAGA 9 cut(s) 99, 219, 313, 438, 498, 501, 504, 507, 671
MfeI CAATTG 2 cut(s) 193, 307
MflI RGATCY 1 cut(s) 286
MluCI AATT 7 cut(s) 193, 307, 340, 365, 385, 442, 778
MlyI GAGTC 1 cut(s) 288
MmeI TCCRAC 1 cut(s) 169
MseI TTAA 3 cut(s) 90, 356, 838
MspA1I CMGCKG 1 cut(s) 812
MspCI CTTAAG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 2 cut(s) 193, 307
MvaI CCWGG 1 cut(s) 291
NdeII GATC 3 cut(s) 157, 286, 769
NlaIII CATG 4 cut(s) 39, 474, 580, 756
NlaIV GGNNCC 2 cut(s) 288, 396
NspI RCATGY 1 cut(s) 474
PaeI GCATGC 1 cut(s) 474
PkrI GCNGC 5 cut(s) 116, 173, 469, 811, 814
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
Ppu21I YACGTR 2 cut(s) 178, 244
PpuMI RGGWCCY 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 288, 396
PspPI GGNCC 2 cut(s) 18, 749
PspPPI RGGWCCY 1 cut(s) 18
PsuI RGATCY 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 7 cut(s) 95, 100, 211, 487, 496, 502, 834
RsaNI GTAC 7 cut(s) 94, 99, 210, 486, 495, 501, 833
SalI GTCGAC 1 cut(s) 76
SaqAI TTAA 3 cut(s) 90, 356, 838
SatI GCNGC 5 cut(s) 115, 172, 468, 810, 813
Sau3AI GATC 3 cut(s) 157, 286, 769
Sau96I GGNCC 2 cut(s) 18, 749
ScaI AGTACT 4 cut(s) 95, 211, 487, 496
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 291
SinI GGWCC 2 cut(s) 18, 749
SmlI CTYRAG 3 cut(s) 89, 422, 828
SmoI CTYRAG 3 cut(s) 89, 422, 828
SpeI ACTAGT 1 cut(s) 482
SphI GCATGC 1 cut(s) 474
Sse9I AATT 7 cut(s) 193, 307, 340, 365, 385, 442, 778
SsiI CCGC 2 cut(s) 114, 171
SspMI CTAG 2 cut(s) 483, 489
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 98, 209, 805
TaiI ACGT 4 cut(s) 180, 246, 362, 594
TaqI TCGA 1 cut(s) 77
TasI AATT 7 cut(s) 193, 307, 340, 365, 385, 442, 778
TatI WGTACW 5 cut(s) 93, 209, 485, 494, 832
TauI GCSGC 2 cut(s) 117, 174
Tru1I TTAA 3 cut(s) 90, 356, 838
Tru9I TTAA 3 cut(s) 90, 356, 838
TscAI CASTG 4 cut(s) 173, 444, 469, 702
TseI GCWGC 3 cut(s) 467, 809, 812
TspDTI ATGAA 1 cut(s) 378
TspGWI ACGGA 2 cut(s) 307, 809
TspRI CASTG 4 cut(s) 173, 444, 469, 702
Vha464I CTTAAG 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 18, 749
XapI RAATTY 2 cut(s) 365, 442
XceI RCATGY 1 cut(s) 474
XcmI CCANNNNNNNNNTGG 1 cut(s) 759
XmiI GTMKAC 1 cut(s) 77
XspI CTAG 2 cut(s) 483, 489
ZrmI AGTACT 4 cut(s) 95, 211, 487, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.