Rorug01G0181500

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0018954
Physical Location & Seq
Forward (+)
25016371 .. 25017636
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0181500.1

Sequence Viewer

Length: 1266 bp
ATGTTTTCAAATGTCAATAACGACACGATGTGCACTAAAAAGAGAAGACCAAGGTCAAGGTGGTCTGAGCTCCCTACTGAACTACTAGAGTTGATCATGAAAAAGCTTGCTTCTGTGGACATCCTTCTCTTTGAAGCTGTCTGTTCTTGTTGGAATCAAGCTGCAAAATCTTATATCTCTGCCCCATATTTCACACTCGACATGCCTCAAACCCCATGGCTTATGATTCCTGGTGGTGAAGAAAACCATGTCCATACTCGCCGCTTCTTCAACCTCGCAGAGTTCAAGTACTACACTATTAAAAACGCGTTTGGAGATATTCCTGATGCCTGGTGTGTCGGTTCGTCACATGGGTGGCTGGTCCTTACGGAGGATAAAGGAACTGCTTGTCTTTTAAATCCGTTTTCTGGAGATAGGATTGAACTCCCATCAATATGGACACTGCATCCAGTCATAAGTAAGGATTATATCGCCAAAGCTGTCACCTCCTGCAAGCCACCATCGTCGTCATCCTCGTCTTCCAGTAAGGACTTCGTTGTTGCAATAATACTCTACTCTAATATGTGGATAACGCAACTTGCTTTCCATAGGCATGGAGACGACGCATGGACTGGCTTCGGCCAGAAGCGTACTGTCTATCATGACGGTATGTTCCACAGTACTAGTGGTCATTTTTTTGCATTGGCAACCGATTGCTCAGTTGAGGTTTGGGACTTGCGGAAATGTTTTCCCATAAAAACACTTCATCTGCAACCTTTTCGACACCGCCAATTAAACAATGACGATGATTTCAAATTTGATATTCAGAATTATTTGGTGGAGTCCTTGGGTGAACTTTTGTTGGTGAGGCGATTTGTAGTGTCGAAGTTTTTTTATCATAAGGACCTATACCCCGATGTTATTCGTCCCTATAGAACCTCTCACTTTTATATCTACAGGCAAAAGTCCACTAGCAAAGGGTTCGAGTGGGAGAAGGTGGAAAGTTTGAAGAGAACTCATTCAATGTCTTTATCTTCCCGGAACTTTACAGAATGTGAAGAGAACTCAATCTACTTCACAGATGATAGGTGGGAAATGAACCCTAATAAATGCTTATCTGGTAAGAAGAGAATTGGCTGTGGGGGTCATGATCTTGGAGTGTACAACGTGAAAGATAATAGTATGAAGCCAATGCCATGTTATTTGACATTTGATCGCTGGATTATAAATCCACCACCCTTTTGGATTGTTCCTGATATAATCCATGGTTTTGGTAATGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

421

Amino Acids

48.79

Weight (kDa)

8.35

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 89 - 382 2.4e-45 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018954)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0342911 RchiOBHm_Chr4g0433091
rosa_multiflora Rmu_sc0000361.1_g000021 Rmu_sc0001291.1_g000003
rosa_rugosa Rorug01G0181500 Rorug01G0181600
rosa_samantha Rh4AG319100 Rh4DG322500
rosa_wichuraiana Rw5G048190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1205
AccII CGCG 1 cut(s) 308
AciI CCGC 3 cut(s) 262, 718, 766
AcoI YGGCCR 1 cut(s) 619
AcsI RAATTY 1 cut(s) 794
AdeI CACNNNGTG 1 cut(s) 30
AfaI GTAC 4 cut(s) 290, 631, 661, 1142
AfiI CCNNNNNNNGG 2 cut(s) 370, 407
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 8 cut(s) 9, 134, 271, 286, 422, 793, 988, 1002
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 2 cut(s) 229, 329
AleI CACNNNNGTG 1 cut(s) 352
AluBI AGCT 6 cut(s) 70, 106, 137, 161, 479, 1263
AluI AGCT 6 cut(s) 70, 106, 137, 161, 479, 1263
Alw21I GWGCWC 2 cut(s) 35, 72
Alw26I GTCTC 1 cut(s) 591
Alw44I GTGCAC 1 cut(s) 31
AoxI GGCC 1 cut(s) 619
ApaLI GTGCAC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 794
Asp700I GAANNNNTTC 1 cut(s) 997
AspS9I GGNCC 2 cut(s) 361, 883
AsuC2I CCSGG 1 cut(s) 1018
AsuHPI GGTGA 4 cut(s) 248, 475, 842, 856
AvaII GGWCC 2 cut(s) 361, 883
BaeGI GKGCMC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 72
BbsI GAAGAC 2 cut(s) 52, 510
Bbv12I GWGCWC 2 cut(s) 35, 72
BbvI GCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 436, 508
BcgI CGANNNNNNTGC 4 cut(s) 740, 774, 943, 977
BciT130I CCWGG 2 cut(s) 231, 331
BclI TGATCA 1 cut(s) 93
BcnI CCSGG 1 cut(s) 1018
BcoDI GTCTC 1 cut(s) 591
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 3 cut(s) 86, 663, 951
BfmI CTRYAG 2 cut(s) 910, 934
BisI GCNGC 2 cut(s) 162, 262
BlsI GCNGC 2 cut(s) 163, 263
BmcAI AGTACT 2 cut(s) 290, 661
Bme1390I CCNGG 3 cut(s) 231, 331, 1018
Bme18I GGWCC 2 cut(s) 361, 883
BmgT120I GGNCC 2 cut(s) 361, 883
BmrFI CCNGG 3 cut(s) 231, 331, 1018
BmsI GCATC 2 cut(s) 316, 454
BoxI GACNNNNGTC 1 cut(s) 52
BpiI GAAGAC 2 cut(s) 52, 510
BpmI CTGGAG 1 cut(s) 429
BpuMI CCSGG 1 cut(s) 1018
BsaJI CCNNGG 4 cut(s) 50, 215, 825, 1243
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 407
Bse1I ACTGG 3 cut(s) 449, 522, 616
BseBI CCWGG 2 cut(s) 231, 331
BseDI CCNNGG 4 cut(s) 50, 215, 825, 1243
BseGI GGATG 3 cut(s) 120, 445, 509
BseLI CCNNNNNNNGG 2 cut(s) 370, 407
BseMII CTCAG 2 cut(s) 57, 711
BseNI ACTGG 3 cut(s) 449, 522, 616
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 621
BsiHKAI GWGCWC 2 cut(s) 35, 72
BsiSI CCGG 1 cut(s) 1018
BslFI GGGAC 2 cut(s) 725, 891
BslI CCNNNNNNNGG 2 cut(s) 370, 407
BsmAI GTCTC 1 cut(s) 591
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 2 cut(s) 725, 891
BsnI GGCC 1 cut(s) 621
Bsp1286I GDGCHC 2 cut(s) 35, 72
Bsp1407I TGTACA 1 cut(s) 1140
Bsp143I GATC 3 cut(s) 93, 1129, 1192
Bsp19I CCATGG 2 cut(s) 215, 1243
BspACI CCGC 3 cut(s) 262, 718, 766
BspANI GGCC 1 cut(s) 621
BspCNI CTCAG 2 cut(s) 58, 710
BspFNI CGCG 1 cut(s) 308
BspHI TCATGA 3 cut(s) 96, 640, 1126
BsrGI TGTACA 1 cut(s) 1140
BsrI ACTGG 3 cut(s) 449, 522, 616
BssECI CCNNGG 4 cut(s) 50, 215, 825, 1243
BssMI GATC 3 cut(s) 93, 1129, 1192
BssT1I CCWWGG 4 cut(s) 50, 215, 825, 1243
Bst2UI CCWGG 2 cut(s) 231, 331
Bst4CI ACNGT 3 cut(s) 634, 647, 659
Bst6I CTCTTC 3 cut(s) 983, 1032, 1100
BstAUI TGTACA 1 cut(s) 1140
BstC8I GCNNGC 2 cut(s) 108, 494
BstDEI CTNAG 2 cut(s) 66, 697
BstDSI CCRYGG 2 cut(s) 215, 1243
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 3 cut(s) 120, 445, 509
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 3 cut(s) 96, 1132, 1195
BstMAI GTCTC 1 cut(s) 591
BstMBI GATC 3 cut(s) 93, 1129, 1192
BstNI CCWGG 2 cut(s) 231, 331
BstNSI RCATGY 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 52
BstSCI CCNGG 3 cut(s) 229, 329, 1016
BstSFI CTRYAG 2 cut(s) 910, 934
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 148
BstV2I GAAGAC 2 cut(s) 52, 510
BstXI CCANNNNNNTGG 4 cut(s) 435, 593, 1221, 1250
BsuRI GGCC 1 cut(s) 621
BtgI CCRYGG 2 cut(s) 215, 1243
BtsCI GGATG 3 cut(s) 120, 445, 509
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 1 cut(s) 440
Cac8I GCNNGC 2 cut(s) 108, 494
CciI TCATGA 3 cut(s) 96, 640, 1126
Cfr13I GGNCC 2 cut(s) 361, 883
CseI GACGC 1 cut(s) 611
Csp6I GTAC 4 cut(s) 289, 630, 660, 1141
CviQI GTAC 4 cut(s) 289, 630, 660, 1141
DdeI CTNAG 2 cut(s) 66, 697
DpnI GATC 3 cut(s) 95, 1131, 1194
DpnII GATC 3 cut(s) 93, 1129, 1192
DraI TTTAAA 1 cut(s) 396
DraIII CACNNNGTG 1 cut(s) 30
EaeI YGGCCR 1 cut(s) 619
Eam1104I CTCTTC 3 cut(s) 983, 1032, 1100
EarI CTCTTC 3 cut(s) 983, 1032, 1100
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 4 cut(s) 50, 215, 825, 1243
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 361, 883
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 883
EcoRII CCWGG 2 cut(s) 229, 329
EcoT14I CCWWGG 4 cut(s) 50, 215, 825, 1243
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 4 cut(s) 50, 215, 825, 1243
Esp3I CGTCTC 1 cut(s) 591
FaqI GGGAC 2 cut(s) 725, 891
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 2 cut(s) 162, 262
FokI GGATG 3 cut(s) 107, 432, 496
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 162, 262
FspBI CTAG 3 cut(s) 86, 663, 951
GluI GCNGC 2 cut(s) 162, 262
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 1 cut(s) 621
HapII CCGG 1 cut(s) 1018
HgaI GACGC 1 cut(s) 611
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 3 cut(s) 154, 226, 821
HpaII CCGG 1 cut(s) 1018
HphI GGTGA 4 cut(s) 248, 475, 842, 856
Hpy166II GTNNAC 5 cut(s) 33, 118, 833, 948, 1141
Hpy188I TCNGA 2 cut(s) 67, 807
Hpy188III TCNNGA 6 cut(s) 97, 323, 408, 641, 1127, 1232
Hpy8I GTNNAC 5 cut(s) 33, 118, 833, 948, 1141
Hpy99I CGWCG 2 cut(s) 508, 605
HpyAV CCTTC 2 cut(s) 134, 967
HpyCH4III ACNGT 3 cut(s) 634, 647, 659
HpyCH4IV ACGT 1 cut(s) 1146
HpyCH4V TGCA 7 cut(s) 33, 164, 445, 492, 542, 680, 751
HpyF3I CTNAG 2 cut(s) 66, 697
HpySE526I ACGT 1 cut(s) 1146
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 3 cut(s) 93, 1129, 1192
LmnI GCTCC 1 cut(s) 75
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 316, 454
MaeI CTAG 3 cut(s) 86, 663, 951
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 2 cut(s) 345, 481
MalI GATC 3 cut(s) 95, 1131, 1194
MboI GATC 3 cut(s) 93, 1129, 1192
MboII GAAGA 8 cut(s) 57, 251, 259, 510, 1000, 1005, 1049, 1117
MhlI GDGCHC 2 cut(s) 35, 72
MluCI AATT 4 cut(s) 770, 794, 808, 1110
MluI ACGCGT 1 cut(s) 306
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 1 cut(s) 131
MnlI CCTC 8 cut(s) 216, 284, 364, 496, 523, 697, 840, 928
MroXI GAANNNNTTC 1 cut(s) 997
MseI TTAA 3 cut(s) 300, 395, 773
MslI CAYNNNNRTG 3 cut(s) 352, 433, 591
MspI CCGG 1 cut(s) 1018
MspR9I CCNGG 3 cut(s) 231, 331, 1018
MvaI CCWGG 2 cut(s) 231, 331
MvnI CGCG 1 cut(s) 308
NciI CCSGG 1 cut(s) 1018
NcoI CCATGG 2 cut(s) 215, 1243
NdeII GATC 3 cut(s) 93, 1129, 1192
NmuCI GTSAC 2 cut(s) 345, 481
NspI RCATGY 1 cut(s) 205
OliI CACNNNNGTG 1 cut(s) 352
PagI TCATGA 3 cut(s) 96, 640, 1126
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PdmI GAANNNNTTC 1 cut(s) 997
PfeI GAWTC 2 cut(s) 154, 226
PfoI TCCNGGA 1 cut(s) 1016
PkrI GCNGC 2 cut(s) 163, 263
PleI GAGTC 1 cut(s) 829
PpsI GAGTC 1 cut(s) 829
PpuMI RGGWCCY 1 cut(s) 883
PshAI GACNNNNGTC 1 cut(s) 52
PsiI TTATAA 1 cut(s) 1205
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 883
Psp6I CCWGG 2 cut(s) 229, 329
PspGI CCWGG 2 cut(s) 229, 329
PspPI GGNCC 2 cut(s) 361, 883
PspPPI RGGWCCY 1 cut(s) 883
PsrI GAACNNNNNNTAC 2 cut(s) 1034, 1066
RsaI GTAC 4 cut(s) 290, 631, 661, 1142
RsaNI GTAC 4 cut(s) 289, 630, 660, 1141
RseI CAYNNNNRTG 3 cut(s) 352, 433, 591
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 300, 395, 773
SatI GCNGC 2 cut(s) 162, 262
Sau3AI GATC 3 cut(s) 93, 1129, 1192
Sau96I GGNCC 2 cut(s) 361, 883
ScaI AGTACT 2 cut(s) 290, 661
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 3 cut(s) 231, 331, 1018
SduI GDGCHC 2 cut(s) 35, 72
SfaNI GCATC 2 cut(s) 316, 454
SfcI CTRYAG 2 cut(s) 910, 934
SinI GGWCC 2 cut(s) 361, 883
SmiMI CAYNNNNRTG 3 cut(s) 352, 433, 591
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 4 cut(s) 770, 794, 808, 1110
SsiI CCGC 3 cut(s) 262, 718, 766
SspMI CTAG 3 cut(s) 86, 663, 951
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 229, 329, 1016
StyI CCWWGG 4 cut(s) 50, 215, 825, 1243
TaaI ACNGT 3 cut(s) 634, 647, 659
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 4 cut(s) 198, 760, 863, 963
TasI AATT 4 cut(s) 770, 794, 808, 1110
TatI WGTACW 3 cut(s) 288, 659, 1140
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 2 cut(s) 154, 226
Tru1I TTAA 3 cut(s) 300, 395, 773
Tru9I TTAA 3 cut(s) 300, 395, 773
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 2 cut(s) 345, 481
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 2 cut(s) 345, 481
TspDTI ATGAA 4 cut(s) 113, 734, 1091, 1178
TspGWI ACGGA 2 cut(s) 383, 390
TspRI CASTG 1 cut(s) 447
VneI GTGCAC 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 361, 883
XagI CCTNNNNNAGG 1 cut(s) 368
XapI RAATTY 1 cut(s) 794
XceI RCATGY 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 3 cut(s) 57, 662, 1218
XmnI GAANNNNTTC 1 cut(s) 997
XspI CTAG 3 cut(s) 86, 663, 951
ZrmI AGTACT 2 cut(s) 290, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.