Rorug01G0182800
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013458
Physical Location & Seq
Reverse (-)
25190574 .. 25193526
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0182800.1

Sequence Viewer

Length: 1557 bp
ATGAGTTCTTTGGGAGGCCTAGTATTCATTCTTTTAAGCATTCTGTTTTTGTTCCTTCTCTTAGTTCTGATCAAGATCTTTCACAAACTATGGTGGAATCCGACTCGCATACAGAAGCTGATGGCTTTGCAAGGAATCAAAGGTCCTTCTTACAGATTTATCCATGGAAATACTAAAGAAATCTCCAGCATGCAAAAGGAAGCCATGAGCAGGCCCAAAAGTTTGTCACATGATATATTTTCTCATGTTCAACCTCATTTCTACTTATGGAGCAGGGCATATGGGAAGAATTTTCTTCAATGGCATGGTTCTCGAGCTCAATTGGTCATTACAGAACCTGAGTTGTGCAAAGAGATACTAAATAACAAAGACAGAACTTTCTTGAAAACCGATTTCCCAATATTTATGAAGAAGCTATTGGGAGATGGACTTGTTACAACTGAAGGTGAAAAATGGGGAAGAATGCGAAAGCTGGCCAACTCTGCCTTCCATGGAGAGAGCTTAAAGAGTCTGATTCCTGCAACTATAGCTAGTGCTGAGTTGATGCTAGAGAGGTGGAAAAGTCATGAAGAAGGGAAAGAGATTGAGATATTTGAAGAATTTAGGTTGTTGACATCAGAAGTGATTTCCAGAACAGCATTTGGCAGCAGCTACTCAGAAGGGAAGAGCATTTTTGACATGTTGATGAAGTTAGCCTTATTAACATTCAAAAATGCTTTCAAAGCTAGGATTCCTATCATCAGTAACATTTTCAAAACCAGCGATGATATTGAAGCACAGAAGCTTGAGAAAGGAGTACATGACTCCATAATAGAGATTGTCAAGAAAAGAGAACAGAAGGCAATGACTATGAGTGAGGAAGAAAAAAGTTTTGGGAGTGATTTTCTTGGATTACTTATGAAAGCTCATCATGATGTCGATGGCAACCAGAGGATTACAGTGGATGATCTAGTTGATGAGTGCAAGACATTTTACTTTGCTGGACAAGAAACCACCAGCAGTTTGCTAGCTTGGACTGTGTTTCTTCTGGCACTCCATACAGATTGGCAAAAGGAAGCAAGAAAAGAAGTCCTGCAATTATTTGGCAAACAAACTCCAAACCCTGATGGCATTTCCAAATTAAAAACAATGGGTATGATCATCAATGAGTCTCTAAGGTTATATCCTCCTGTTGTGTCCATATATAGGAAAGTCGCACGAGAAGTTAGACTCGGAAAACTCCTTGTTCCAGCTGATCTTGAAATTCTTATTCCATGTTTGGCCCTTCAGCATGAGCCTCAATTCTGGGGACAAGATGTGGAATTGTTCAAACCAGAGAGATTCTCAGAAGGGGTTGCTAAAGCTACCAACAATAACATGGCCACATTCTTACCCTTTGGAATGGGACCTAGAAATTGTGTGGGATACAACTTTGCAACCACTGAAGCCAAGATTGCTCTGGCAATGATTCTACAACGCTACGCCTTCACACTTTCCCCGTGTTATGTTCACTCACCCTTTCGGCTTCTTACAGTTCGGCCACAACATGGAATTCAAGTAATGCTACACTCACTGTGA

Protein Analysis

518

Amino Acids

59.17

Weight (kDa)

9.13

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 90 - 493 5.1e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60400
fragaria_vesca FvH4_7g09760
malus_domestica MD00G1041100.v1.1 MD09G1283700.v1.1
prunus_persica Prupe.2G125600_v2.0.a1
pyrus_communis pycom02g18190 pycom09g19160
rosa_chinensis RchiOBHm_Chr1g0346761
rosa_laevigata RLG00000028781
rosa_multiflora Rmu_sc0003232.1_g000007
rosa_roxburghii Rroxscaffold_4G00308420
rosa_rugosa Rorug01G0182700 Rorug01G0182800
rosa_samantha Rh1AG201200 Rh1BG167400 Rh1CG185900 Rh1DG198200
rosa_wichuraiana Rw1G016860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1526
AcoI YGGCCR 3 cut(s) 474, 1359, 1517
AcsI RAATTY 4 cut(s) 289, 599, 1242, 1530
AcuI CTGAAG 3 cut(s) 462, 1250, 1443
AfaI GTAC 1 cut(s) 798
AfiI CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
AflIII ACRYGT 1 cut(s) 678
AjuI GAANNNNNNNTTGG 6 cut(s) 401, 433, 470, 502, 855, 887
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 1155
AlwNI CAGNNNCTG 2 cut(s) 118, 338
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 6 cut(s) 16, 212, 474, 1260, 1359, 1517
ApeKI GCWGC 2 cut(s) 645, 648
ApoI RAATTY 4 cut(s) 289, 599, 1242, 1530
AspS9I GGNCC 4 cut(s) 143, 213, 1261, 1385
AsuHPI GGTGA 2 cut(s) 458, 1485
AsuNHI GCTAGC 1 cut(s) 1006
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 2 cut(s) 143, 1385
BalI TGGCCA 2 cut(s) 476, 1361
BanII GRGCYC 1 cut(s) 319
BauI CACGAG 1 cut(s) 1197
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 657, 660
BccI CCATC 4 cut(s) 115, 419, 914, 1100
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciVI GTATCC 1 cut(s) 1397
BclI TGATCA 2 cut(s) 69, 1137
BcoDI GTCTC 1 cut(s) 1155
BfaI CTAG 7 cut(s) 20, 531, 548, 726, 950, 1007, 1389
BfmI CTRYAG 1 cut(s) 525
BfuI GTATCC 1 cut(s) 1397
BglII AGATCT 1 cut(s) 75
BisI GCNGC 2 cut(s) 646, 649
BlsI GCNGC 2 cut(s) 647, 650
Bme18I GGWCC 2 cut(s) 143, 1385
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 4 cut(s) 143, 213, 1261, 1385
BmiI GGNNCC 1 cut(s) 1386
BmsI GCATC 1 cut(s) 534
BmtI GCTAGC 1 cut(s) 1010
BplI GAGNNNNNCTC 2 cut(s) 1307, 1339
BpmI CTGGAG 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 806
BsaBI GATNNNNATC 2 cut(s) 74, 734
BsaJI CCNNGG 2 cut(s) 163, 490
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
Bse3DI GCAATG 2 cut(s) 849, 1449
Bse8I GATNNNNATC 2 cut(s) 74, 734
BseDI CCNNGG 2 cut(s) 163, 490
BseGI GGATG 1 cut(s) 949
BseJI GATNNNNATC 2 cut(s) 74, 734
BseLI CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
BseMI GCAATG 2 cut(s) 849, 1449
BseMII CTCAG 4 cut(s) 330, 528, 669, 1338
BseXI GCAGC 2 cut(s) 657, 660
BshFI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 312
BslFI GGGAC 2 cut(s) 1302, 1398
BslI CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
BsmAI GTCTC 1 cut(s) 1155
BsmFI GGGAC 2 cut(s) 1302, 1398
BsmI GAATGC 2 cut(s) 39, 468
BsnI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 5 cut(s) 69, 75, 946, 1137, 1234
Bsp19I CCATGG 2 cut(s) 163, 490
BspANI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BspCNI CTCAG 4 cut(s) 331, 529, 668, 1337
BspHI TCATGA 2 cut(s) 565, 910
BspLI GGNNCC 1 cut(s) 1386
BspOI GCTAGC 1 cut(s) 1010
BspQI GCTCTTC 1 cut(s) 659
BsrDI GCAATG 2 cut(s) 849, 1449
BssECI CCNNGG 2 cut(s) 163, 490
BssMI GATC 5 cut(s) 69, 75, 946, 1137, 1234
BssSI CACGAG 1 cut(s) 1197
BssT1I CCWWGG 2 cut(s) 163, 490
Bst2BI CACGAG 1 cut(s) 1197
Bst4CI ACNGT 4 cut(s) 940, 1018, 1513, 1554
Bst6I CTCTTC 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 191, 212, 474, 1008
BstDEI CTNAG 6 cut(s) 61, 339, 537, 655, 1154, 1324
BstDSI CCRYGG 2 cut(s) 163, 490
BstF5I GGATG 1 cut(s) 949
BstKTI GATC 5 cut(s) 72, 78, 949, 1140, 1237
BstMAI GTCTC 1 cut(s) 1155
BstMBI GATC 5 cut(s) 69, 75, 946, 1137, 1234
BstMWI GCNNNNNNNGC 4 cut(s) 482, 527, 722, 1433
BstNSI RCATGY 2 cut(s) 193, 682
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 657, 660
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuI GTATCC 1 cut(s) 1397
BsuRI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BtgI CCRYGG 2 cut(s) 163, 490
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 1 cut(s) 949
BtsIMutI CAGTG 3 cut(s) 945, 1419, 1550
Cac8I GCNNGC 4 cut(s) 191, 212, 474, 1008
CaiI CAGNNNCTG 2 cut(s) 118, 338
CciI TCATGA 2 cut(s) 565, 910
Cfr13I GGNCC 4 cut(s) 143, 213, 1261, 1385
Csp6I GTAC 1 cut(s) 797
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 6 cut(s) 61, 339, 537, 655, 1154, 1324
DpnI GATC 5 cut(s) 71, 77, 948, 1139, 1236
DpnII GATC 5 cut(s) 69, 75, 946, 1137, 1234
EaeI YGGCCR 3 cut(s) 474, 1359, 1517
Eam1104I CTCTTC 1 cut(s) 659
EarI CTCTTC 1 cut(s) 659
Ecl136II GAGCTC 1 cut(s) 317
Eco130I CCWWGG 2 cut(s) 163, 490
Eco147I AGGCCT 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 2 cut(s) 143, 1385
Eco53kI GAGCTC 1 cut(s) 317
Eco57I CTGAAG 3 cut(s) 462, 1250, 1443
Eco88I CYCGRG 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 317
EcoO109I RGGNCCY 2 cut(s) 143, 1385
EcoRI GAATTC 1 cut(s) 1530
EcoT14I CCWWGG 2 cut(s) 163, 490
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 163, 490
FalI AAGNNNNNCTT 2 cut(s) 680, 712
FaqI GGGAC 2 cut(s) 1302, 1398
FauNDI CATATG 1 cut(s) 280
FbaI TGATCA 2 cut(s) 69, 1137
Fnu4HI GCNGC 2 cut(s) 646, 649
FokI GGATG 1 cut(s) 956
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 2 cut(s) 646, 649
FspBI CTAG 7 cut(s) 20, 531, 548, 726, 950, 1007, 1389
GluI GCNGC 2 cut(s) 646, 649
GsuI CTGGAG 1 cut(s) 169
HaeIII GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 782
HphI GGTGA 2 cut(s) 458, 1485
Hpy166II GTNNAC 2 cut(s) 612, 1489
Hpy188I TCNGA 7 cut(s) 69, 102, 513, 619, 658, 1214, 1327
Hpy188III TCNNGA 8 cut(s) 73, 312, 382, 566, 630, 823, 911, 1238
Hpy8I GTNNAC 2 cut(s) 612, 1489
HpyCH4III ACNGT 4 cut(s) 940, 1018, 1513, 1554
HpyCH4V TGCA 7 cut(s) 130, 193, 348, 521, 963, 1075, 1415
HpyF10VI GCNNNNNNNGC 4 cut(s) 482, 527, 722, 1433
HpyF3I CTNAG 6 cut(s) 61, 339, 537, 655, 1154, 1324
Ksp22I TGATCA 2 cut(s) 69, 1137
Kzo9I GATC 5 cut(s) 69, 75, 946, 1137, 1234
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 1 cut(s) 270
Lsp1109I GCAGC 2 cut(s) 657, 660
LweI GCATC 1 cut(s) 534
MaeI CTAG 7 cut(s) 20, 531, 548, 726, 950, 1007, 1389
MaeIII GTNAC 3 cut(s) 225, 433, 743
MalI GATC 5 cut(s) 71, 77, 948, 1139, 1236
MboI GATC 5 cut(s) 69, 75, 946, 1137, 1234
MboII GAAGA 9 cut(s) 287, 298, 421, 471, 581, 608, 676, 872, 1016
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 2 cut(s) 476, 1361
MluNI TGGCCA 2 cut(s) 476, 1361
MlyI GAGTC 5 cut(s) 97, 517, 797, 1157, 1203
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 7 cut(s) 8, 264, 546, 850, 924, 1176, 1287
Mox20I TGGCCA 2 cut(s) 476, 1361
MscI TGGCCA 2 cut(s) 476, 1361
MseI TTAA 4 cut(s) 35, 503, 701, 1121
MslI CAYNNNNRTG 2 cut(s) 683, 912
Msp20I TGGCCA 2 cut(s) 476, 1361
MspA1I CMGCKG 1 cut(s) 1232
MunI CAATTG 1 cut(s) 320
Mva1269I GAATGC 2 cut(s) 39, 468
MwoI GCNNNNNNNGC 4 cut(s) 482, 527, 722, 1433
NcoI CCATGG 2 cut(s) 163, 490
NdeI CATATG 1 cut(s) 280
NdeII GATC 5 cut(s) 69, 75, 946, 1137, 1234
NheI GCTAGC 1 cut(s) 1006
NlaIV GGNNCC 1 cut(s) 1386
NmuCI GTSAC 1 cut(s) 225
NspI RCATGY 2 cut(s) 193, 682
PaeI GCATGC 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 312
PagI TCATGA 2 cut(s) 565, 910
PceI AGGCCT 1 cut(s) 18
PciI ACATGT 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 659
PctI GAATGC 2 cut(s) 39, 468
PfeI GAWTC 6 cut(s) 97, 135, 514, 730, 1320, 1447
PflMI CCANNNNNTGG 1 cut(s) 1526
PkrI GCNGC 2 cut(s) 647, 650
PleI GAGTC 5 cut(s) 97, 516, 797, 1156, 1203
PpsI GAGTC 5 cut(s) 97, 516, 797, 1156, 1203
PpuMI RGGWCCY 2 cut(s) 143, 1385
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 319
Psp5II RGGWCCY 2 cut(s) 143, 1385
PspN4I GGNNCC 1 cut(s) 1386
PspPI GGNCC 4 cut(s) 143, 213, 1261, 1385
PspPPI RGGWCCY 2 cut(s) 143, 1385
PstNI CAGNNNCTG 2 cut(s) 118, 338
PsuI RGATCY 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 1232
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 683, 912
SacI GAGCTC 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 4 cut(s) 35, 503, 701, 1121
SatI GCNGC 2 cut(s) 646, 649
Sau3AI GATC 5 cut(s) 69, 75, 946, 1137, 1234
Sau96I GGNCC 4 cut(s) 143, 213, 1261, 1385
SchI GAGTC 5 cut(s) 97, 517, 797, 1157, 1203
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 1 cut(s) 525
Sfr274I CTCGAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 143, 1385
SlaI CTCGAG 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 683, 912
SmlI CTYRAG 2 cut(s) 312, 785
SmoI CTYRAG 2 cut(s) 312, 785
SphI GCATGC 1 cut(s) 193
SseBI AGGCCT 1 cut(s) 18
SspI AATATT 1 cut(s) 402
SspMI CTAG 7 cut(s) 20, 531, 548, 726, 950, 1007, 1389
SstI GAGCTC 1 cut(s) 319
StuI AGGCCT 1 cut(s) 18
StyI CCWWGG 2 cut(s) 163, 490
TaaI ACNGT 4 cut(s) 940, 1018, 1513, 1554
TaqI TCGA 2 cut(s) 313, 918
TatI WGTACW 1 cut(s) 796
TfiI GAWTC 6 cut(s) 97, 135, 514, 730, 1320, 1447
Tru1I TTAA 4 cut(s) 35, 503, 701, 1121
Tru9I TTAA 4 cut(s) 35, 503, 701, 1121
TscAI CASTG 3 cut(s) 945, 1426, 1557
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 2 cut(s) 645, 648
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 5 cut(s) 16, 422, 582, 701, 914
TspRI CASTG 3 cut(s) 945, 1426, 1557
Van91I CCANNNNNTGG 1 cut(s) 1526
VpaK11BI GGWCC 2 cut(s) 143, 1385
XapI RAATTY 4 cut(s) 289, 599, 1242, 1530
XceI RCATGY 2 cut(s) 193, 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 1354, 1435
XhoI CTCGAG 1 cut(s) 312
XspI CTAG 7 cut(s) 20, 531, 548, 726, 950, 1007, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.