Rorug01G0201200

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0016613
Physical Location & Seq
Forward (+)
28068320 .. 28073998
5679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0201200.1

Sequence Viewer

Length: 918 bp
ATGGGTTCCTGCGTCTGCTTACGTCAGCACTGCCGCTACGGTGGTTTGGGCGGTGCATTTCAAAAAGGGGTTGTTGCGCCAGGTTTAACGGCGCCGTTTTATCAGTATCACAGGAACAGAAGAAGAGTGGAGGCCTGTGTTTCTACTGGGTTTAAGGAACAAGAGCTGAGGAGCCCGGACCTTGTGGCCTTGGAATATGCTGATCTTAATCTCACCCACAATGTTTCCCAGGAACTGGGTCACGTCAGAATTAGGCAACATGTCAACCCTCTTAGCTCTTCTTTATCTGTGCCTGTGGAAGTGCCTGATTGGAATCAAGTGTTCAGGGACCCAACATTGCCGCTCATGGTGGACATTGGAAGTGGTAGTGGTAGATTTCTCATGTGGCTGGCGAAAAGAAACTTAGTTATGAGGAATTATTTGGGACTGGAAATAAGGCAGAAACTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCTTACTAATATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACCTACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCACGGACAGGTGTTTATACAGTCCGATGTACTTGACATCGCACTTGAGATGAGAAACCAATTTGATTCTCTGCCTCATGTTCTGAAGCACATAGATGAAATCGAATCCAGCGTGTTGTGTGACAGTGAGGGATGGTTGTTGAGCAACCCAATGGGAAAAAGGACGGAAAGAGAAATTCATGCTGAATTTGAAGGGGCAAAAATTTACAGGAGAGTGTACCAGAAGCAAGTACAGAAGGCTCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

305

Amino Acids

34.76

Weight (kDa)

9.28

Isoelectric Point (pI)

54.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 114 - 251 4.8e-31 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016613)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60650
fragaria_vesca FvH4_7g11140
malus_domestica MD07G1116200.v1.1
prunus_persica Prupe.2G138500_v2.0.a1
rosa_laevigata RLG00000028599
rosa_multiflora Rmu_sc0003385.1_g000027 Rmu_ssc0000150.1_g000013
rosa_roxburghii Rroxscaffold_4G00306050
rosa_rugosa Rorug01G0201200
rosa_samantha Rh1AG218600 Rh1BG184000 Rh1CG203100 Rh1DG213800
rosa_wichuraiana Rw1G018570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 1 cut(s) 235
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 542
AciI CCGC 3 cut(s) 34, 51, 341
AclWI GGATC 1 cut(s) 581
AcsI RAATTY 3 cut(s) 843, 854, 870
AcuI CTGAAG 1 cut(s) 773
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 4 cut(s) 627, 699, 887, 900
AfiI CCNNNNNNNGG 2 cut(s) 235, 676
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 3 cut(s) 62, 573, 860
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 79, 228, 550
AluBI AGCT 4 cut(s) 166, 276, 478, 665
AluI AGCT 4 cut(s) 166, 276, 478, 665
Alw26I GTCTC 1 cut(s) 610
AlwI GGATC 1 cut(s) 581
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 2 cut(s) 132, 186
ApoI RAATTY 3 cut(s) 843, 854, 870
AspLEI GCGC 2 cut(s) 79, 94
AspS9I GGNCC 3 cut(s) 178, 328, 554
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 205
AvaII GGWCC 3 cut(s) 178, 328, 554
BaeGI GKGCMC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 176
BbvCI CCTCAGC 1 cut(s) 167
BccI CCATC 1 cut(s) 795
BceAI ACGGC 2 cut(s) 79, 105
BciT130I CCWGG 3 cut(s) 81, 230, 552
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 1 cut(s) 610
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 2 cut(s) 34, 341
BlsI GCNGC 2 cut(s) 35, 342
Bme1390I CCNGG 4 cut(s) 81, 176, 230, 552
Bme18I GGWCC 3 cut(s) 178, 328, 554
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 3 cut(s) 178, 328, 554
BmiI GGNNCC 5 cut(s) 7, 93, 173, 329, 330
BmrFI CCNGG 4 cut(s) 81, 176, 230, 552
BmrI ACTGGG 2 cut(s) 156, 245
BmsI GCATC 2 cut(s) 552, 616
BmuI ACTGGG 2 cut(s) 156, 245
Bpu10I CCTNAGC 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 176
BsaBI GATNNNNATC 2 cut(s) 207, 312
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 4 cut(s) 189, 228, 550, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 676
Bse1I ACTGG 4 cut(s) 151, 240, 432, 540
Bse3DI GCAATG 1 cut(s) 335
Bse8I GATNNNNATC 2 cut(s) 207, 312
BseBI CCWGG 3 cut(s) 81, 230, 552
BseDI CCNNGG 4 cut(s) 189, 228, 550, 670
BseGI GGATG 1 cut(s) 806
BseJI GATNNNNATC 2 cut(s) 207, 312
BseLI CCNNNNNNNGG 2 cut(s) 235, 676
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 4 cut(s) 151, 240, 432, 540
BseRI GAGGAG 1 cut(s) 184
BseSI GKGCMC 1 cut(s) 584
BseYI CCCAGC 1 cut(s) 478
BshFI GGCC 2 cut(s) 134, 188
BshNI GGYRCC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 176
BslFI GGGAC 2 cut(s) 341, 438
BslI CCNNNNNNNGG 2 cut(s) 235, 676
BsmAI GTCTC 1 cut(s) 610
BsmBI CGTCTC 1 cut(s) 610
BsmFI GGGAC 2 cut(s) 341, 438
BsnI GGCC 2 cut(s) 134, 188
Bsp1286I GDGCHC 2 cut(s) 176, 584
Bsp1407I TGTACA 1 cut(s) 625
Bsp143I GATC 2 cut(s) 202, 586
BspACI CCGC 3 cut(s) 34, 51, 341
BspANI GGCC 2 cut(s) 134, 188
BspCNI CTCAG 1 cut(s) 159
BspLI GGNNCC 5 cut(s) 7, 93, 173, 329, 330
BspPI GGATC 1 cut(s) 581
BspQI GCTCTTC 1 cut(s) 283
BspT107I GGYRCC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 335
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 4 cut(s) 151, 240, 432, 540
BssECI CCNNGG 4 cut(s) 189, 228, 550, 670
BssMI GATC 2 cut(s) 202, 586
BssNI GRCGYC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 81, 230, 552
Bst4CI ACNGT 3 cut(s) 41, 690, 794
Bst6I CTCTTC 2 cut(s) 118, 283
BstACI GRCGYC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 3 cut(s) 167, 272, 403
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 1 cut(s) 806
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 2 cut(s) 79, 94
BstKTI GATC 2 cut(s) 205, 589
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 2 cut(s) 202, 586
BstNI CCWGG 3 cut(s) 81, 230, 552
BstNSI RCATGY 1 cut(s) 263
BstSCI CCNGG 4 cut(s) 79, 174, 228, 550
BstSLI GKGCMC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 2 cut(s) 134, 188
BtgI CCRYGG 1 cut(s) 670
BtgZI GCGATG 1 cut(s) 691
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 806
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 3 cut(s) 28, 557, 799
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 1 cut(s) 235
CfoI GCGC 2 cut(s) 79, 94
Cfr13I GGNCC 3 cut(s) 178, 328, 554
Csp6I GTAC 4 cut(s) 626, 698, 886, 899
CviAII CATG 6 cut(s) 260, 346, 382, 502, 746, 848
CviQI GTAC 4 cut(s) 626, 698, 886, 899
DdeI CTNAG 3 cut(s) 167, 272, 403
DinI GGCGCC 1 cut(s) 93
DpnI GATC 2 cut(s) 204, 588
DpnII GATC 2 cut(s) 202, 586
Eam1104I CTCTTC 2 cut(s) 118, 283
EarI CTCTTC 2 cut(s) 118, 283
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 3 cut(s) 178, 328, 554
Eco57I CTGAAG 1 cut(s) 773
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 3 cut(s) 79, 228, 550
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 176
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 610
FaeI CATG 6 cut(s) 263, 349, 385, 505, 749, 851
FaqI GGGAC 2 cut(s) 341, 438
FatI CATG 6 cut(s) 259, 345, 381, 501, 745, 847
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 34, 341
FokI GGATG 1 cut(s) 813
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 34, 341
GlaI GCGC 2 cut(s) 78, 93
GluI GCNGC 2 cut(s) 34, 341
GsaI CCCAGC 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 2 cut(s) 134, 188
HapII CCGG 1 cut(s) 176
HhaI GCGC 2 cut(s) 79, 94
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 6 cut(s) 263, 349, 385, 505, 749, 851
Hin6I GCGC 2 cut(s) 77, 92
HinP1I GCGC 2 cut(s) 77, 92
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 4 cut(s) 313, 644, 734, 773
HpaII CCGG 1 cut(s) 176
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 4 cut(s) 265, 352, 543, 886
Hpy188I TCNGA 3 cut(s) 248, 694, 753
Hpy8I GTNNAC 4 cut(s) 265, 352, 543, 886
HpyAV CCTTC 2 cut(s) 854, 898
HpyCH4III ACNGT 3 cut(s) 41, 690, 794
HpyCH4IV ACGT 3 cut(s) 22, 243, 454
HpyCH4V TGCA 2 cut(s) 56, 509
HpyF3I CTNAG 3 cut(s) 167, 272, 403
HpySE526I ACGT 3 cut(s) 22, 243, 454
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 6 cut(s) 263, 349, 385, 505, 749, 851
HspAI GCGC 2 cut(s) 77, 92
KasI GGCGCC 1 cut(s) 91
KflI GGGWCCC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 202, 586
LguI GCTCTTC 1 cut(s) 283
LmnI GCTCC 2 cut(s) 171, 670
LweI GCATC 2 cut(s) 552, 616
MaeII ACGT 3 cut(s) 22, 243, 454
MaeIII GTNAC 2 cut(s) 239, 788
MalI GATC 2 cut(s) 204, 588
MbiI CCGCTC 1 cut(s) 343
MboI GATC 2 cut(s) 202, 586
MboII GAAGA 3 cut(s) 132, 135, 270
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 2 cut(s) 176, 584
MluCI AATT 7 cut(s) 249, 415, 658, 728, 843, 854, 870
Mly113I GGCGCC 1 cut(s) 92
MnlI CCTC 6 cut(s) 124, 162, 279, 405, 753, 790
MseI TTAA 6 cut(s) 86, 153, 207, 471, 528, 597
MslI CAYNNNNRTG 1 cut(s) 762
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 4 cut(s) 81, 176, 230, 552
MvaI CCWGG 3 cut(s) 81, 230, 552
NarI GGCGCC 1 cut(s) 92
NciI CCSGG 1 cut(s) 176
NdeII GATC 2 cut(s) 202, 586
NlaIII CATG 6 cut(s) 263, 349, 385, 505, 749, 851
NlaIV GGNNCC 5 cut(s) 7, 93, 173, 329, 330
NmuCI GTSAC 2 cut(s) 239, 788
NspI RCATGY 1 cut(s) 263
PceI AGGCCT 1 cut(s) 134
PciI ACATGT 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 777
PfeI GAWTC 4 cut(s) 313, 644, 734, 773
PflMI CCANNNNNTGG 1 cut(s) 235
PkrI GCNGC 2 cut(s) 35, 342
PluTI GGCGCC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 328
PscI ACATGT 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 3 cut(s) 79, 228, 550
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 3 cut(s) 79, 228, 550
PspN4I GGNNCC 5 cut(s) 7, 93, 173, 329, 330
PspPI GGNCC 3 cut(s) 178, 328, 554
PspPPI RGGWCCY 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 235
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 4 cut(s) 627, 699, 887, 900
RsaNI GTAC 4 cut(s) 626, 698, 886, 899
RseI CAYNNNNRTG 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 283
SaqAI TTAA 6 cut(s) 86, 153, 207, 471, 528, 597
SatI GCNGC 2 cut(s) 34, 341
Sau3AI GATC 2 cut(s) 202, 586
Sau96I GGNCC 3 cut(s) 178, 328, 554
ScrFI CCNGG 4 cut(s) 81, 176, 230, 552
SduI GDGCHC 2 cut(s) 176, 584
SfaNI GCATC 2 cut(s) 552, 616
SfoI GGCGCC 1 cut(s) 93
SinI GGWCC 3 cut(s) 178, 328, 554
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 7 cut(s) 249, 415, 658, 728, 843, 854, 870
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 3 cut(s) 34, 51, 341
SspDI GGCGCC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 4 cut(s) 79, 174, 228, 550
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 3 cut(s) 41, 690, 794
TaiI ACGT 3 cut(s) 25, 246, 457
TaqI TCGA 1 cut(s) 771
TasI AATT 7 cut(s) 249, 415, 658, 728, 843, 854, 870
TatI WGTACW 3 cut(s) 625, 697, 898
TauI GCSGC 2 cut(s) 36, 343
TfiI GAWTC 4 cut(s) 313, 644, 734, 773
Tru1I TTAA 6 cut(s) 86, 153, 207, 471, 528, 597
Tru9I TTAA 6 cut(s) 86, 153, 207, 471, 528, 597
TscAI CASTG 3 cut(s) 35, 564, 799
TseFI GTSAC 2 cut(s) 239, 788
Tsp45I GTSAC 2 cut(s) 239, 788
TspDTI ATGAA 4 cut(s) 490, 513, 780, 836
TspGWI ACGGA 3 cut(s) 579, 687, 848
TspRI CASTG 3 cut(s) 35, 564, 799
Van91I CCANNNNNTGG 1 cut(s) 235
VpaK11BI GGWCC 3 cut(s) 178, 328, 554
XapI RAATTY 3 cut(s) 843, 854, 870
XceI RCATGY 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.