Rorug02G0007300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018698
Physical Location & Seq
Reverse (-)
647629 .. 650401
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0007300.1

Sequence Viewer

Length: 1038 bp
ATGGCTAGTGGAGAAGAAGTGAGGAACAAGCAGGTGATATTCGGAGACTATATCACCGGCTTCCCCAAAGAATCCGACCTCAAATTGACCACTGCCACTACCAAACTCAAGCTTCCAGAAGGTTCGACGGGCCTCCTCGTCAAGAACCTCTACTTGTCCTGCGATCCTTACATGCGAAGCCGTATGACCAAGCACGACAGGCCCACTTATGTCCAATCCTTCACGCCCGGCTCGCCTGTAACTGGTTTGGGAGTGGCTAAAGTCCTGGAATCTGGGGATCCAAAGTTTAAGCCAGGGGACTTGGTTTGGGGTCACACTGGTTGGGAAGAATATAGTGTCATCACCACAGAGTCTCTGATTAAGATTCACCACACTGATTTGCCTCTCTCTTACTATACTGGACTTCTCGGTATGCCTGGAATGACTGCTTATGCTGGTTTTTATGAGATCTGCTCTCCTAAGAAAGGAGAGACAGTCTACATTTCAGCCGCATCTGGAGCGGTAGGTCAGCTTGTTGGCCAATTTGCAAAGTTAACAGGTTGTTATGTTGTTGGGAGTGCTGGAAGCAAGGAAAAGGTTGATCTGCTGAAGAACAAATTTGGATTTGACGAGGCTTTCAACTATAAAGACGAACCTGACCTAGATGCAGCTTTAAGAAGGTACTTTCCAGATGGTATTGACATTTACTTTGAAAATGTGGGGGGAAAGATGCTTGATGCAGTGCTACCAAATATGAGGCTGAAAGGGCGAATAGCTGTGTGTGGGATGATCTCACAGTACAACCTCGAGCGGCCTGAAGGCATACATAATTTGATGTCTCTGATTGTTAAGCAGGTCCGAATGGAAGGTTTCCTGGTCTTCAGTTACTATCATCTGTACGGAAAGTTTCTTGAAACAGTGTTGCCTTACATAAAAGAAGGGAAGATGACATATGTGGAAGATGTAGTTGAAGGCCTTGAGAATGCTCCAGCGGCTCTAATTGGGCTCTTTTCTGGCCGCAATGTGGGAAAGCAGGTGGTTGTAGTTTCCAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

345

Amino Acids

38.12

Weight (kDa)

6.62

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 10 - 122 4.1e-27 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 167 - 291 3.2e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 340 2.2e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 22, 1003
Acc36I ACCTGC 3 cut(s) 22, 823, 1003
AccBSI CCGCTC 2 cut(s) 500, 790
AccI GTMKAC 1 cut(s) 477
AciI CCGC 5 cut(s) 489, 500, 790, 971, 997
AclWI GGATC 3 cut(s) 158, 272, 285
AcoI YGGCCR 2 cut(s) 517, 994
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 3 cut(s) 608, 816, 844
AfaI GTAC 3 cut(s) 662, 779, 878
AfiI CCNNNNNNNGG 3 cut(s) 242, 464, 1003
AgsI TTSAA 4 cut(s) 619, 692, 893, 950
AjnI CCWGG 4 cut(s) 264, 292, 415, 852
AluBI AGCT 4 cut(s) 112, 511, 650, 755
AluI AGCT 4 cut(s) 112, 511, 650, 755
Alw26I GTCTC 4 cut(s) 39, 357, 464, 822
AlwI GGATC 3 cut(s) 158, 272, 285
Ama87I CYCGRG 1 cut(s) 785
AoxI GGCC 6 cut(s) 130, 200, 517, 791, 952, 994
ApeKI GCWGC 1 cut(s) 647
ApoI RAATTY 1 cut(s) 596
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AspS9I GGNCC 3 cut(s) 130, 201, 835
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 4 cut(s) 46, 46, 334, 359
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 1 cut(s) 835
BalI TGGCCA 1 cut(s) 519
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 987
BbsI GAAGAC 1 cut(s) 850
BbvI GCAGC 1 cut(s) 659
BccI CCATC 1 cut(s) 665
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 4 cut(s) 266, 294, 417, 854
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 4 cut(s) 39, 357, 464, 822
BfaI CTAG 2 cut(s) 6, 641
BfuAI ACCTGC 3 cut(s) 22, 823, 1003
BglII AGATCT 1 cut(s) 447
BisI GCNGC 5 cut(s) 489, 648, 791, 972, 997
BlsI GCNGC 5 cut(s) 490, 649, 792, 973, 998
Bme1390I CCNGG 5 cut(s) 228, 266, 294, 417, 854
Bme18I GGWCC 1 cut(s) 835
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 3 cut(s) 130, 201, 835
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 5 cut(s) 228, 266, 294, 417, 854
BmsI GCATC 4 cut(s) 500, 634, 699, 706
BpiI GAAGAC 1 cut(s) 850
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 2 cut(s) 516, 951
BpuEI CTTGAG 2 cut(s) 92, 977
BpuMI CCSGG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 293
BsaXI ACNNNNNCTCC 3 cut(s) 33, 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 242, 464, 1003
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 3 cut(s) 247, 322, 403
Bse3DI GCAATG 1 cut(s) 1006
BseBI CCWGG 4 cut(s) 266, 294, 417, 854
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 1 cut(s) 771
BseLI CCNNNNNNNGG 3 cut(s) 242, 464, 1003
BseMI GCAATG 1 cut(s) 1006
BseNI ACTGG 3 cut(s) 247, 322, 403
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 659
BshFI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 2 cut(s) 57, 228
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 3 cut(s) 242, 464, 1003
BsmAI GTCTC 4 cut(s) 39, 357, 464, 822
BsmFI GGGAC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 967
BsnI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BsoBI CYCGRG 1 cut(s) 785
Bsp1286I GDGCHC 1 cut(s) 987
Bsp143I GATC 5 cut(s) 163, 277, 447, 580, 768
BspACI CCGC 5 cut(s) 489, 500, 790, 971, 997
BspANI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 3 cut(s) 22, 823, 1003
BspPI GGATC 3 cut(s) 158, 272, 285
BsrBI CCGCTC 2 cut(s) 500, 790
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 3 cut(s) 247, 322, 403
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 5 cut(s) 163, 277, 447, 580, 768
Bst2UI CCWGG 4 cut(s) 266, 294, 417, 854
Bst4CI ACNGT 3 cut(s) 475, 777, 898
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 459
BstENI CCTNNNNNAGG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 771
BstKTI GATC 5 cut(s) 166, 280, 450, 583, 771
BstMAI GTCTC 4 cut(s) 39, 357, 464, 822
BstMBI GATC 5 cut(s) 163, 277, 447, 580, 768
BstMWI GCNNNNNNNGC 5 cut(s) 199, 232, 497, 745, 971
BstNI CCWGG 4 cut(s) 266, 294, 417, 854
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 5 cut(s) 226, 264, 292, 415, 852
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 277, 447
BstYI RGATCY 2 cut(s) 277, 447
BsuRI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BtsCI GGATG 1 cut(s) 771
BtsI GCAGTG 2 cut(s) 90, 726
BtsIMutI CAGTG 5 cut(s) 90, 315, 372, 726, 903
BveI ACCTGC 3 cut(s) 22, 823, 1003
Cac8I GCNNGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 130, 201, 835
Csp6I GTAC 3 cut(s) 661, 778, 877
CviAII CATG 1 cut(s) 172
CviQI GTAC 3 cut(s) 661, 778, 877
DdeI CTNAG 1 cut(s) 459
DpnI GATC 5 cut(s) 165, 279, 449, 582, 770
DpnII GATC 5 cut(s) 163, 277, 447, 580, 768
EaeI YGGCCR 2 cut(s) 517, 994
Eco147I AGGCCT 1 cut(s) 954
Eco24I GRGCYC 1 cut(s) 987
Eco47I GGWCC 1 cut(s) 835
Eco57I CTGAAG 3 cut(s) 608, 816, 844
Eco88I CYCGRG 1 cut(s) 785
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoRII CCWGG 4 cut(s) 264, 292, 415, 852
EcoT38I GRGCYC 1 cut(s) 987
FaeI CATG 1 cut(s) 175
FaqI GGGAC 1 cut(s) 311
FatI CATG 1 cut(s) 171
FauNDI CATATG 1 cut(s) 931
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 5 cut(s) 489, 648, 791, 972, 997
FokI GGATG 1 cut(s) 778
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 5 cut(s) 489, 648, 791, 972, 997
FspBI CTAG 2 cut(s) 6, 641
GluI GCNGC 5 cut(s) 489, 648, 791, 972, 997
GsuI CTGGAG 2 cut(s) 516, 951
HaeIII GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
HapII CCGG 2 cut(s) 57, 228
Hin1II CATG 1 cut(s) 175
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 4 cut(s) 71, 269, 350, 364
HpaI GTTAAC 1 cut(s) 534
HpaII CCGG 2 cut(s) 57, 228
HphI GGTGA 4 cut(s) 46, 46, 334, 359
Hpy166II GTNNAC 2 cut(s) 478, 534
Hpy188I TCNGA 5 cut(s) 44, 76, 357, 822, 839
Hpy188III TCNNGA 6 cut(s) 116, 142, 495, 668, 890, 1029
Hpy8I GTNNAC 2 cut(s) 478, 534
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 7 cut(s) 113, 229, 651, 791, 839, 911, 944
HpyCH4III ACNGT 3 cut(s) 475, 777, 898
HpyCH4V TGCA 3 cut(s) 527, 647, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 232, 497, 745, 971
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 534
Kzo9I GATC 5 cut(s) 163, 277, 447, 580, 768
LmnI GCTCC 2 cut(s) 497, 970
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 4 cut(s) 500, 634, 699, 706
MaeI CTAG 2 cut(s) 6, 641
MaeIII GTNAC 3 cut(s) 238, 311, 863
MalI GATC 5 cut(s) 165, 279, 449, 582, 770
MbiI CCGCTC 2 cut(s) 500, 790
MboI GATC 5 cut(s) 163, 277, 447, 580, 768
MboII GAAGA 6 cut(s) 26, 338, 601, 850, 934, 950
MflI RGATCY 2 cut(s) 277, 447
MhlI GDGCHC 1 cut(s) 987
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 5 cut(s) 83, 521, 596, 808, 978
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 15, 89, 143, 146, 158, 393, 604, 729, 794
Mox20I TGGCCA 1 cut(s) 519
MscI TGGCCA 1 cut(s) 519
MseI TTAA 5 cut(s) 288, 360, 533, 653, 828
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 2 cut(s) 57, 228
MspR9I CCNGG 5 cut(s) 228, 266, 294, 417, 854
Mva1269I GAATGC 1 cut(s) 967
MvaI CCWGG 4 cut(s) 266, 294, 417, 854
MwoI GCNNNNNNNGC 5 cut(s) 199, 232, 497, 745, 971
NciI CCSGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 931
NdeII GATC 5 cut(s) 163, 277, 447, 580, 768
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 1 cut(s) 175
PaeR7I CTCGAG 1 cut(s) 785
PaqCI CACCTGC 2 cut(s) 22, 1003
PceI AGGCCT 1 cut(s) 954
PctI GAATGC 1 cut(s) 967
PfeI GAWTC 3 cut(s) 71, 269, 364
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 5 cut(s) 490, 649, 792, 973, 998
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 4 cut(s) 264, 292, 415, 852
PspGI CCWGG 4 cut(s) 264, 292, 415, 852
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 3 cut(s) 130, 201, 835
PspXI VCTCGAGB 1 cut(s) 785
PsuI RGATCY 2 cut(s) 277, 447
RsaI GTAC 3 cut(s) 662, 779, 878
RsaNI GTAC 3 cut(s) 661, 778, 877
SaqAI TTAA 5 cut(s) 288, 360, 533, 653, 828
SatI GCNGC 5 cut(s) 489, 648, 791, 972, 997
Sau3AI GATC 5 cut(s) 163, 277, 447, 580, 768
Sau96I GGNCC 3 cut(s) 130, 201, 835
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 5 cut(s) 228, 266, 294, 417, 854
SduI GDGCHC 1 cut(s) 987
SfaNI GCATC 4 cut(s) 500, 634, 699, 706
Sfr274I CTCGAG 1 cut(s) 785
SinI GGWCC 1 cut(s) 835
SlaI CTCGAG 1 cut(s) 785
SmlI CTYRAG 3 cut(s) 107, 785, 956
SmoI CTYRAG 3 cut(s) 107, 785, 956
Sse9I AATT 5 cut(s) 83, 521, 596, 808, 978
SseBI AGGCCT 1 cut(s) 954
SsiI CCGC 5 cut(s) 489, 500, 790, 971, 997
SspMI CTAG 2 cut(s) 6, 641
StuI AGGCCT 1 cut(s) 954
StyD4I CCNGG 5 cut(s) 226, 264, 292, 415, 852
TaaI ACNGT 3 cut(s) 475, 777, 898
TaqI TCGA 2 cut(s) 125, 786
TasI AATT 5 cut(s) 83, 521, 596, 808, 978
TatI WGTACW 1 cut(s) 777
TauI GCSGC 4 cut(s) 491, 793, 974, 999
TfiI GAWTC 3 cut(s) 71, 269, 364
Tru1I TTAA 5 cut(s) 288, 360, 533, 653, 828
Tru9I TTAA 5 cut(s) 288, 360, 533, 653, 828
TscAI CASTG 5 cut(s) 97, 322, 379, 726, 903
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 1 cut(s) 311
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 5 cut(s) 97, 322, 379, 726, 903
VpaK11BI GGWCC 1 cut(s) 835
XagI CCTNNNNNAGG 1 cut(s) 462
XapI RAATTY 1 cut(s) 596
XceI RCATGY 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 2 cut(s) 6, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.